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Open AccessArticle

HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of Cucumis melo Seeds in Gentamicin-Induced Renal Damage

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Department of Pharmacology, University College of Pharmacy, University of the Punjab, Lahore, Punjab 54000, Pakistan
2
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Punjab 38000, Pakistan
3
Department of Pathology, Aziz Fatima Medical and Dental College, Faisalabad, Punjab 38000, Pakistan
*
Authors to whom correspondence should be addressed.
Medicina 2019, 55(4), 107; https://doi.org/10.3390/medicina55040107
Received: 6 February 2019 / Revised: 2 March 2019 / Accepted: 10 April 2019 / Published: 15 April 2019
Background and objectives: Cucumis melo, of family Cucurbitaceae, has traditionally been used to treat variety of kidney disorders. However to best of our knowledge there is no scientific study available that validates its renaoprotective uses. Therefore, this study aimed to evaluate nephroprotective effects of hydroalcoholic extract of Cucumis melo seeds (CMHE) and to identify its phytoconstituents. Materials and Methods: HPLC was performed to identify key phytochemicals of CMHE. Gentamicin (100 mg/kg/day, i.p) was administered to induce nephrotoxicity in Swiss albino mice for 8 days. Gentamicin (100 mg/kg/day, i.p) and oral CMHE were co-administered to mice at doses of 250 and 500 mg/kg to evaluate protective effects of CMHE. Normal control group mice were administered normal saline. Changes in body weights, biochemical and histopathological studies were conducted to establish nephroprotective effects of CMHE. Results: HPLC analysis indicated presence of quercetin, m-coumaric acid, gallic acid, chlorogenic acid, and trans-4-hydroxy-3-methoxy cinnamic acid in CMHE. Mice treated with CMHE showed significant increase in body weight and decrease in kidney weight as compared with toxic control group. Dose-dependent significant decrease in total blood urea nitrogen, serum creatinine, serum urea, and uric acid levels were observed in CMHE-treated groups as compared with toxic control group. Histopathological analysis of CMHE-treated groups showed improvement in kidney structures as compared with toxic control group. Conclusions: Biochemical, histopathological, and phytochemical screening of hydroalcoholic extract of Cucumis melo seeds suggest that it has nephroprotective potential. Furthermore, standardization of extract against identified phytochemicals, as well as long-term toxicological studies are suggested before commencement of clinical trials. View Full-Text
Keywords: nephroprotective effect; Cucumis melo seeds; hydroalcoholic extract; gentamicin; HPLC analysis nephroprotective effect; Cucumis melo seeds; hydroalcoholic extract; gentamicin; HPLC analysis
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Saleem, M.; Javed, F.; Asif, M.; Kashif Baig, M.; Arif, M. HPLC Analysis and In Vivo Renoprotective Evaluation of Hydroalcoholic Extract of Cucumis melo Seeds in Gentamicin-Induced Renal Damage. Medicina 2019, 55, 107.

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