Next Article in Journal
Role of BRAF in Hepatocellular Carcinoma: A Rationale for Future Targeted Cancer Therapies
Previous Article in Journal
Perfusion Index Analysis in Patients Presenting to the Emergency Department Due to Synthetic Cannabinoid Use
Open AccessArticle

The Role of C21orf91 in Herpes Simplex Virus Keratitis

1
Department of Ophthalmology, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania
2
Department of Immunology and Allergology, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania
3
Department of Dental and Oral Pathology, Medical Academy, Lithuanian University of Health Sciences, LT-44307 Kaunas, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2019, 55(12), 753; https://doi.org/10.3390/medicina55120753
Received: 10 July 2019 / Revised: 10 October 2019 / Accepted: 19 November 2019 / Published: 20 November 2019
Background and Objectives: This paper aims to describe the single nucleotide polymorphisms (SNPs) of C21orf91 rs1062202 and rs10446073 in patients with herpetic keratitis by evaluating corneal sub-basal nerves, as well as the density of Langerhans cells (LC) and endothelium cells (EC) during the acute phase of the disease. Materials and Methods: A prospective clinical study included 260 subjects: 70 with herpetic eye disease, 101 with previous history of herpes labialis—but no history of herpetic eye disease—and 89 with no history of any herpes simplex virus (HSV) diseases. All subjects underwent a complete ophthalmological examination including in vivo laser scanning confocal microscopy (LSCM) of the central cornea. C21orf91 rs1062202 and rs10446073 were genotyped using the real-time polymerase chain reaction (PCR) method with the Rotor-Gene Q real-time PCR quantification system. SNPs were determined using TaqMan genotyping assay, according to the manufacturer’s manual. Results: The C21orf91 rs10446073 genotype GT was more frequent in the HSV keratitis group, compared with healthy controls (20.0% vs. 7.9%), OR 2.929[1.11–7.716] (p < 0.05). The rs10446073 genotype TT was more frequent in healthy controls (12.4% vs. 1.4%), OR 22.0[2.344–260.48] (p < 0.05). The rs10446073 genotype GT increased the risk of EC density being less than 2551.5 cell/mm2, OR 2.852[1.248–6.515] (p < 0.05). None of the SNPs and their genotypes influenced the LC density and corneal sub-basal nerve parameters (p > 0.05). Conclusions: Our study reports a new association between herpetic keratitis and human gene C21orf91, with the rs10446073 genotype GT being more common in herpetic keratitis patients and increasing the risk for the disease by a factor of 2.9. View Full-Text
Keywords: herpetic keratitis; C21orf91; confocal microscopy herpetic keratitis; C21orf91; confocal microscopy
Show Figures

Figure 1

MDPI and ACS Style

Danileviciene, V.; Zemaitiene, R.; Gintauskiene, V.M.; Nedzelskiene, I.; Zaliuniene, D. The Role of C21orf91 in Herpes Simplex Virus Keratitis. Medicina 2019, 55, 753.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop