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Open AccessArticle

Molecular Characterization of Uropathogenic Escherichia coli Reveals Emergence of Drug Resistant O15, O22 and O25 Serogroups

1
Life Sciences Centre, Vilnius University, Vilnius 10257, Lithuania
2
Institute for Infectious Diseases and Pathogenic Microbiology, Prienai 59115, Lithuania
3
Institute for Microbiology and Virology, Lithuanian University of Health Sciences, Kaunas 47181, Lithuania
4
Biological Research Center, Lithuanian University of Health Sciences, Kaunas 47181, Lithuania
5
Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, NY 10065, USA
6
National Laboratory for Public Health Surveillance, Kaunas 44153, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2019, 55(11), 733; https://doi.org/10.3390/medicina55110733
Received: 4 October 2019 / Revised: 7 November 2019 / Accepted: 8 November 2019 / Published: 11 November 2019
Background and Objectives: Uropathogenic Escherichia coli (UPEC) are common pathogens causing urinary tract infections (UTIs). We aimed to investigate the relationship among clinical manifestation, serogroups, phylogenetic groups, and antimicrobial resistance among UPEC. Materials and Methods: One-hundred Escherichia coli isolates recovered from urine and ureteral scrapings were used for the study. The prevalence of antimicrobial resistance was determined by using European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommendations. E. coli serogroups associated with UTI, as well as phylogenetic diversity were analyzed using multiplex PCR reactions. Results: Eighty-seven strains (87%) were isolated from females, while 13 (13%) from males. A high frequency of resistance to cephalosporins (43%) and fluoroquinolones (31%) was observed. Among UTI-associated serogroups O15 (32.8%), O22 (23.4%), and O25 (15.6%) were dominant and demonstrated elevated resistance rates. The E. coli phylogenetic group B2 was most common. These observations extended to pregnant patients with asymptomatic bacteriuria. Conclusions: Due to high rates of resistance, strategies using empirical therapy of second-generation cephalosporins and fluoroquinolones should be reconsidered in this population. View Full-Text
Keywords: E. coli; Lithuania; serogroups; UPEC; urinary tract infections E. coli; Lithuania; serogroups; UPEC; urinary tract infections
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Prakapaite, R.; Saab, F.; Planciuniene, R.; Petraitis, V.; Walsh, T.J.; Petraitiene, R.; Semoskaite, R.; Baneviciene, R.; Kalediene, L.; Kavaliauskas, P. Molecular Characterization of Uropathogenic Escherichia coli Reveals Emergence of Drug Resistant O15, O22 and O25 Serogroups. Medicina 2019, 55, 733.

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