Next Article in Journal
Impact of Left Ventricular Diastolic Dysfunction and Biomarkers on Pulmonary Hypertension in Patients with Severe Aortic Stenosis
Previous Article in Journal
Current Research on the Safety of Pyrethroids Used as Insecticides
Article Menu
Issue 4 (September) cover image

Export Article

Open AccessArticle
Medicina 2018, 54(4), 62;

Impact of Gender and Age on Hyperthermia-Induced Changes in Respiration of Liver Mitochondria

Department of Biochemistry, Faculty of Natural Sciences, Vytautas Magnus University, LT-44404 Kaunas, Lithuania
Author to whom correspondence should be addressed.
Received: 5 July 2018 / Revised: 28 August 2018 / Accepted: 29 August 2018 / Published: 31 August 2018
Full-Text   |   PDF [1862 KB, uploaded 31 August 2018]   |  


Aim: This study aimed to compare hyperthermia-induced changes in respiration and generation of reactive oxygen species (ROS) in liver mitochondria derived from animals of different gender and age. Methods: The effects of hyperthermia (40–47 °C) on oxidation of different substrates and ROS production were estimated in mitochondria isolated from the liver of male and female rats of the 1–1.5, 3–4, or 6–7 months age. Results: Gender-dependent differences in response of respiration to hyperthermia were the highest at 3–4 months of age, less so at 6–7 months of age, and only minor at juvenile age. Mild hyperthermia (40–42 °C) stimulated pyruvate + malate oxidation in mitochondria of females, but inhibited in mitochondria of males in the 3–4 month age group. The resistance of mitochondrial membrane to hyperthermia was the highest at 3–4 month males, and the lowest in the 6–7 month age group. Inhibition of glutamate + malate oxidation by hyperthermia was caused by thermal inactivation of glutamate dehydrogenase. ROS generation at 37 °C was higher at 1–1.5 month of age, but the increase in ROS generation with rise in temperature in this age group was the smallest, and the strongest in 6–7 month old animals of both genders. Conclusions: The response to hyperthermia varies during the first 6–7 months of life of experimental animals: stronger gender dependence is characteristic at 3–4 months of age, while mitochondria from 6–7 months animals are less resistant to hyperthermia. View Full-Text
Keywords: age; gender; hyperthermia; liver mitochondria; reactive oxygen species; respiration age; gender; hyperthermia; liver mitochondria; reactive oxygen species; respiration

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Šilkūnienė, G.; Žūkienė, R.; Naučienė, Z.; Degutytė-Fomins, L.; Mildažienė, V. Impact of Gender and Age on Hyperthermia-Induced Changes in Respiration of Liver Mitochondria. Medicina 2018, 54, 62.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics



[Return to top]
Medicina EISSN 1010-660X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top