Distribution of human papillomavirus type 16 variants in Lithuanian women with cervical cancer
Materials and methods: A total of 122 HPV 16 positive cervical samples (invasive cancer and cervical intraepithelial neoplasia) were investigated and sequenced to identify different variants. HPV 16 was detected using type specific PCR, exact sequence of the virus was obtained by viral DNA sequencing.
Results: Adequate HPV sequence was detected in 106 cases from 122 (86.9% of all cases). After histological confirmation, 96 cases were included in the final analysis. In 33 cases (34.4%) HPV 16 prototype was detected; in 50 cases (52.1%), L83V variant; and in remaining 13 cases (13.5%), multivariant of HPV 16. The frequency of L83V variant in invasive cancer and carcinoma in situ samples was the same (66.7% and 62.0%, respectively; P = 0.696). Of analyzed multivariants, 10 were attributed to the European phylogenetic line; 1, to the North American, and 1, to the Asian-American. One sample was not attributed to any of the known phylogenetic lines.
Conclusions: The European HPV 16 L83V variant is usually associated with high risk of cervical cancer among women. However, statistically significant difference was not achieved when comparing difference of L83V variants between investigated groups and in HPV 16 L83V variant and prototype distribution in CIN3/Ca in situ and cancer.
Gudlevičienė, Ž.; Stumbrytė, A.; Juknė, G.; Simanavičienė, V.; Žvirblienė, A. Distribution of human papillomavirus type 16 variants in Lithuanian women with cervical cancer. Medicina 2015, 51, 328-335.
Gudlevičienė Ž, Stumbrytė A, Juknė G, Simanavičienė V, Žvirblienė A. Distribution of human papillomavirus type 16 variants in Lithuanian women with cervical cancer. Medicina. 2015; 51(6):328-335.Chicago/Turabian Style
Gudlevičienė, Živilė; Stumbrytė, Aušra; Juknė, Gabrielė; Simanavičienė, Vaida; Žvirblienė, Aurelija. 2015. "Distribution of human papillomavirus type 16 variants in Lithuanian women with cervical cancer." Medicina 51, no. 6: 328-335.