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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Evaluation of low-dose proton beam radiation efficiency in MIA PaCa-2 pancreatic cancer cell line vitality and H2AX formation

1
Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania
2
Laboratory of Immunology, National Cancer Institute, Vilnius, Lithuania
3
Department of Nuclear Research, Institute of Physics, Center for Physical Sciences and Technology, Vilnius, Lithuania
4
Laboratory of Molecular Oncology, National Cancer Institute, Vilnius, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2015, 51(5), 302-306; https://doi.org/10.1016/j.medici.2015.10.002
Received: 30 April 2014 / Accepted: 13 October 2015 / Published: 6 November 2015
Background and objective: The aim of this study was to evaluate the efficiency of proton beam irradiation in pancreatic cancer cell line MIA PaCa-2 and its role in the cell cycle, apoptosis, and formation of histone gH2AX in different reparation times (72-h follow-up).
Material and methods: The MIA PaCa-2 pancreatic carcinoma cell line was irradiated with 1.6- Gy proton beam. After irradiation, cell viability was measured colorimetrically, and the cell cycle, apoptosis, and gH2AX expression were evaluated on a FACScan cytometer.
Results: Low-dose proton beam irradiation had an effect on the MIA PaCa-2 tumor cell line already 1 h after exposure, but maximal lethality was reached after 72 h postirradiation with a cell viability rate of 24%. The cell cycle went into partial G1/0 arrest, and was released after 72 h. The expression of gH2AX was strong and its levels were significantly elevated as late as 48 h post radiation. The apoptosis levels increased with post radiation incubation time to reach 79% after 72 h.
Conclusions: Our data demonstrate that low-doses proton beam irradiation had an effect on MIA PaCa-2 pancreatic carcinoma cell line. Full extent of irradiation had an impact only 24 h postirradiation, triggering DNA arrested cell cycle in G1/0 phase. Formed DNA DSBs were found to be repaired via the NHEJ pathway mechanism within 72 h. Unsuccessful repaired DSBs induced apoptotic cell death. After 72 h reparation processes were completed, and cell cycle was released from arrest in G1/0 phase.
Keywords: Proton irradiation; MIA PaCa-2; Gamma H2AX; Non-homologous end joining; Double strand breaks Proton irradiation; MIA PaCa-2; Gamma H2AX; Non-homologous end joining; Double strand breaks
MDPI and ACS Style

Liubavičiūtė, A.; Kraśko, J.A.; Mlynska, A.; Lagzdina, J.; Sužiedėlis, K.; Pašukonienė, V. Evaluation of low-dose proton beam radiation efficiency in MIA PaCa-2 pancreatic cancer cell line vitality and H2AX formation. Medicina 2015, 51, 302-306.

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