Accumulation and biological effects of cobalt ferrite nanoparticles in human pancreatic and ovarian cancer cells
Materials and methods: Co-SPIONs were produced by Massart's co-precipitation method. The cells were treated with Co-SPIONs at three different concentrations (0.095, 0.48, and 0.95 μg/mL) for 24 and 48 h. Cell viability and proliferation were analyzed after treatment. The stemlike properties of cells were assessed by investigating the cell clonogenicity and expression of cancer stem cell-associated markers, including CD24/ESA in A2780 cell line and CD44/ALDH1 in MiaPaCa2 cell line. Magnetically activated cell sorting was used for the separation of magnetically labeled and unlabeled cells.
Results: Both cancer cell lines accumulated Co-SPIONs, however differences in response to nanoparticles were observed between MiaPaCa2 and A2780 cell. In particular, A2780 cells were more sensitive to exposition to Co-SPIONs than MiaPaCa2 cells, indicating that a safe concentration of nanoparticles must be estimated individually for a particular cell type. Higher doses of Co-SPIONs decreased both the clonogenicity and ESA marker expression in A2780 cells.
Conclusions: Co-SPIONs are not cytotoxic to cancer cells, at least when used at a concentration of up to 0.95 μg/mL. Co-SPIONs have a dose-dependent effect on the clonogenic potential and ESA marker expression in A2780 cells. Magnetic detection of low concentrations of Co-SPIONS in cancer cells is a promising tool for further applications of these nanoparticles in cancer diagnosis and treatment; however, extensive research in this field is needed.
Pašukonienė, V.; Mlynska, A.; Steponkienė, S.; Poderys, V.; Matulionytė, M.; Karabanovas, V.; Statkutė, U.; Purvinienė, R.; Kraśko, J.A.; Jagminas, A.; Kurtinaitienė, M.; Strioga, M.; Rotomskis, R. Accumulation and biological effects of cobalt ferrite nanoparticles in human pancreatic and ovarian cancer cells. Medicina 2014, 50, 237-244.
Pašukonienė V, Mlynska A, Steponkienė S, Poderys V, Matulionytė M, Karabanovas V, Statkutė U, Purvinienė R, Kraśko JA, Jagminas A, Kurtinaitienė M, Strioga M, Rotomskis R. Accumulation and biological effects of cobalt ferrite nanoparticles in human pancreatic and ovarian cancer cells. Medicina. 2014; 50(4):237-244.Chicago/Turabian Style
Pašukonienė, Vita; Mlynska, Agata; Steponkienė, Simona; Poderys, Vilius; Matulionytė, Marija; Karabanovas, Vitalijus; Statkutė, Urtė; Purvinienė, Rasa; Kraśko, Jan Aleksander; Jagminas, Arūnas; Kurtinaitienė, Marija; Strioga, Marius; Rotomskis, Ričardas. 2014. "Accumulation and biological effects of cobalt ferrite nanoparticles in human pancreatic and ovarian cancer cells." Medicina 50, no. 4: 237-244.