Nevomelanocytic atypia detection by in vivo reflectance confocal microscopy
Materials and methods: A total of 138 melanocytic skin lesions comprising 25 melanocytic nevi, 69 dysplastic nevi and 44 melanomas were analyzed by means of dermoscopy, in vivo RCM and routine histopathology. In vivo RCM images were analyzed for the arrangement of keratinocytes in epidermis, pagetoid cells and junctional melanocytic nests and correlated refractivity aspects of nests with histopathology.
Results: Separately and all together taken the in vivo RCM features of melanocytic atypia were significant in differential diagnosis of benign and malignant melanocytic skin lesions, though none of the features was significant in discriminating nevi without cytologic atypia of dysplastic nevi. In vivo RCM feature of dense cell clusters corresponded with melanin containing nevomelanocytes on histopathology though exact correspon- dence of non-homogeneous and atypical sparse cell clusters remained questionable.
Conclusions: Nevus with histopathologically confirmed nevomelanocytic atypia (dysplastic nevus) could not be distinguished from nevus without atypia using analyzed in vivo RCM features of melanocytic atypia. More accurate diagnostics by means of in vivo RCM needs further investigation on reflectance of single and nested cutaneous melanocytes in benign and malignant skin lesions.
Vaišnorienė, I.; Rotomskis, R.; Kulvietis, V.; Eidukevičius, R.; Žalgevičienė, V.; Laurinavičienė, A.; Venius, J.; Didžiapetrienė, J. Nevomelanocytic atypia detection by in vivo reflectance confocal microscopy. Medicina 2014, 50, 209-215.
Vaišnorienė I, Rotomskis R, Kulvietis V, Eidukevičius R, Žalgevičienė V, Laurinavičienė A, Venius J, Didžiapetrienė J. Nevomelanocytic atypia detection by in vivo reflectance confocal microscopy. Medicina. 2014; 50(4):209-215.Chicago/Turabian Style
Vaišnorienė, Ingrida; Rotomskis, Ričardas; Kulvietis, Vytautas; Eidukevičius, Rimantas; Žalgevičienė, Violeta; Laurinavičienė, Aida; Venius, Jonas; Didžiapetrienė, Janina. 2014. "Nevomelanocytic atypia detection by in vivo reflectance confocal microscopy." Medicina 50, no. 4: 209-215.