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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

CpG Island Methylation of the MLH1, MGMT, DAPK, and CASP8 Genes in Cancerous and Adjacent Noncancerous Stomach Tissues

1
Institute of Oncology, Medical Academy, Lithuanian University of Health Sciences
2
Institute for Digestive Research, Medical Academy, Lithuanian University of Health Sciences
3
Department of Surgery, Medical Academy, Hospital of Lithuanian University of Health Sciences
4
Department of Pathologic Anatomy, Medical Academy, Lithuanian University of Health Sciences
5
Department of Anaesthesiology, Medical Academy, Lithuanian University of Health Sciences, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2013, 49(8), 56; https://doi.org/10.3390/medicina49080056
Received: 18 April 2013 / Accepted: 30 August 2013 / Published: 4 September 2013
Background and Objective. Many factors are involved in the development of gastric adenocarcinoma. The CpG island methylation of apoptosis and mismatch repair genes by the loss of their function is important in gastric adenocarcinoma. The aim of this study was to determine the methylation frequency of MLH1, MGMT, CASP8, and DAPK in cancerous and adjacent noncancerous stomach tissues, to determine possible associations with the selected clinicopathological characteristics, and to identify possible correlation between the methylation of individual genes.
Material and Methods. The methylation status of MLH1, MGMT, DAPK, and CASP8 was investigated in 69 patients with gastric adenocarcinoma by using methylation-specific polymerase chain reaction. The associations between patients’ clinical characteristics and methylation status were assessed.
Results
. The methylation frequency of the MLH1, DAPK, MGMT, and CASP8 gene promoters in cancerous and adjacent noncancerous tissues was 31.9% and 27.5%; 47.8% and 46.4%; 36.2% and 44.9%; and 5.8% and 5.8%, respectively, but the differences were not significant. There was no significant association between the methylation status of the mentioned genes and clinicopathological characteristics, such as age, sex, tumor type by the Lauren classification, degree of differentiation G, and TNM staging. An inverse correlation between the methylation of the DAPK and MLH1 gene promoters in cancerous and surrounding noncancerous tissues was found.
Conclusions. The methylation of the MLH1, MGMT, DAPK, and CASP8 genes was found to occur both in cancerous and noncancerous stomach tissues. These findings provide additional insights into gene methylation patterns in gastric adenocarcinoma.
Keywords: stomach cancer; methylation; MLH1; DAPK; MGMT; CASP8 stomach cancer; methylation; MLH1; DAPK; MGMT; CASP8
MDPI and ACS Style

Kupčinskaitė-Noreikienė, R.; Skiecevičienė, J.; Jonaitis, L.; Ugenskienė, R.; Kupčinskas, J.; Markelis, R.; Baltrėnas, V.; Linas; Semakina, I.; Grižas, S.; Juozaitytė, E. CpG Island Methylation of the MLH1, MGMT, DAPK, and CASP8 Genes in Cancerous and Adjacent Noncancerous Stomach Tissues. Medicina 2013, 49, 56.

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