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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

The Effect of Polyscias filicifolia Bailey Biomass Tincture on the Protein Synthesis Process in the Heterogeneous System From the Isolated Pig Heart

1
Department of Biochemistry, Lithuanian University of Health Sciences
2
Laboratory of Molecular Cardiology, Institute of Cardiology, Lithuanian University of Health Sciences, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2013, 49(6), 44; https://doi.org/10.3390/medicina49060044
Received: 7 December 2012 / Accepted: 30 May 2013 / Published: 4 June 2013
Background and Objective. An insufficient supply of oxygen to the heart influences the process of protein synthesis. The aim of this study was to determine the effect of the Polyscias filicifolia Bailey biomass tincture on the protein synthesis process in a heterogeneous translation system from the isolated pig heart.
Materials and Methods
. The effect of anoxia was evaluated after 20- and 90-minute anoxia. With the aim to determine the effect of Polyscias, the pig hearts were perfused with a buffer containing the Polyscias filicifolia Bailey biomass tincture. To determine the rate and the level of translation, the incorporation of [14C]-leucine into translational products in a cell-free system was measured.
Results
. The protein synthesis level decreased by 23%–42% when the translation system containing cytosol from the anoxic heart was used. When the translation system containing a ribosomal fraction after 20-minutes anoxia was used, the protein synthesis level was the same as in the control. In the case of 90-minute anoxia, it decreased by 16%. The protein synthesis rate and the level in the translation system containing cytosol from the heart after 20-minute anoxic perfusion with the buffer containing Polyscias was the same as in the control.
Conclusions
. A decrease in the protein synthesis rate and the level after 20-minute anoxia was determined by changes in cytosol. On the other hand, 90-minute anoxia caused changes in cytosol and the ribosomal fraction. The Polyscias filicifolia Bailey biomass tincture restored the protein synthesis process acting on the components of the translation system in cytosol and the ribosomal fraction.
Keywords: anoxia; protein synthesis; pig heart; Polyscias filicifolia Bailey anoxia; protein synthesis; pig heart; Polyscias filicifolia Bailey
MDPI and ACS Style

Kašauskas, A.; Mongirdienė, A. The Effect of Polyscias filicifolia Bailey Biomass Tincture on the Protein Synthesis Process in the Heterogeneous System From the Isolated Pig Heart. Medicina 2013, 49, 44.

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