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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Peripheral Blood Th17 Cells and Neutrophils in Dermatophagoides pteronyssinus-induced Early- and Late-Phase Asthmatic Response

1
Department of Pulmonology and Immunology, Medical Academy, Lithuanian University of Health Sciences
2
Laboratory of Pulmonology, Department of Pulmonology and Immunology, Medical Academy, Lithuanian University of Health Sciences
3
Department of Laboratory Medicine, Medical Academy, Lithuanian University of Health Sciences, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2012, 48(9), 66; https://doi.org/10.3390/medicina48090066
Received: 19 December 2011 / Accepted: 17 July 2012 / Published: 22 July 2012
Background and Objective. Biphasic cellular immune reactions, which follow allergen inhalation, are a specific feature of inflammation in allergic asthma. The aim of this study was to determine the changes in the percentage of peripheral blood Th17 cells and neutrophil functions after Dermatophagoides pteronyssinus-induced early- and late-phase asthmatic response in patients with allergic asthma.
Material and Methods.
A total of 19 patients with allergic asthma were examined. Eleven patients developed an isolated early-phase asthmatic response (EAR), whereas 8 developed both earlyand late-phase (dual) asthmatic responses (DAR) after the bronchial challenge with Dermatophagoides pteronyssinus. The control group included 15 healthy subjects. Peripheral blood collection was performed 24 hours before as well as 7 and 24 hours after the bronchial challenge. The percentage of Th17 cells, and chemotaxis and apoptosis of neutrophils were analyzed by flow cytometry. The serum IL-8 and IL-17 levels were determined by ELISA.
Results
. After the bronchial challenge, the percentage of Th17 and IL-17 levels increased considerably 7 and 24 hours after the challenge in both groups of patients. Moreover, 24 hours after the challenge, the percentage of Th17 cells and IL-17 levels were significantly higher in the patients with the DAR than those with the EAR or healthy controls. Seven and 24 hours after the challenge, neutrophil chemotaxis was greater in the patients with the DAR as compared with those with the EAR and healthy controls as well. The apoptotic activity of neutrophils was lower 24 hours after the challenge in the patients with the DAR than those with the EAR.
Conclusions
. Dermatophagoides pteronyssinus-induced early- and late-phase asthmatic response in patients with allergic asthma was found to be accompanied by an increased percentage of peripheral blood Th17 cells and elevated serum IL-17 levels as well as altered neutrophil functions.
Keywords: allergic asthma; early-phase asthmatic response; late-phase asthmatic response; dual asthmatic response; Th17 cells; neutrophil functions allergic asthma; early-phase asthmatic response; late-phase asthmatic response; dual asthmatic response; Th17 cells; neutrophil functions
MDPI and ACS Style

Bajoriūnienė, I.; Malakauskas, K.; Lavinskienė, S.; Jeroch, J.; Gasiūnienė, E.; Vitkauskienė, A.; Sakalauskas, R. Peripheral Blood Th17 Cells and Neutrophils in Dermatophagoides pteronyssinus-induced Early- and Late-Phase Asthmatic Response. Medicina 2012, 48, 66.

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