A Novel Approach for Nucleic Acid Delivery Into Cancer Cells
Material and Methods. Reporter plasmid pcDNA3.1LacZ DNA was used in combination with Lipofectamine2000 reagent and superparamagnetic nanoparticles CombiMag. The effects of incubation time under a dynamic magnetic field and a rotation frequency of magnets on transfection efficiency for PC3 cell line were determined. Alternatively, lipofection and liposomal magnetofection were carried out. Transfection efficiency of delivery methods was estimated by β-galactosidase staining; cell viability, by acridine orange/ethidium bromide staining.
Results. Liposomal magnetofection under a dynamic gradient magnetic field demonstrated the highest transfection efficiency: it was greater by almost 21% and 42% in comparison with liposomal magnetofection and lipofection, respectively. The optimal incubation time under dynamic magnetic field and the optimal magnet rotation frequency were 5 minutes and 5 rpm, respectively. Liposomal magnetofection under a dynamic gradient magnetic field was less cytotoxic (7%) than that under a permanent magnetic field (17%) and lipofection (11%).
Conclusions. Our new approach, based on the use of a dynamic gradient magnetic field, enhanced the transfection efficiency and had a less cytotoxic effect on prostate cancer cells in comparison with the standard magnetofection and lipofection.
Vainauska, D.; Kozireva, S.; Karpovs, A.; Čistjakovs, M.; Bariševs, M. A Novel Approach for Nucleic Acid Delivery Into Cancer Cells. Medicina 2012, 48, 48.
Vainauska D, Kozireva S, Karpovs A, Čistjakovs M, Bariševs M. A Novel Approach for Nucleic Acid Delivery Into Cancer Cells. Medicina. 2012; 48(6):48.Chicago/Turabian Style
Vainauska, Dace; Kozireva, Svetlana; Karpovs, Andrejs; Čistjakovs, Maksims; Bariševs, Mihails. 2012. "A Novel Approach for Nucleic Acid Delivery Into Cancer Cells." Medicina 48, no. 6: 48.