Mitochondrial Membrane Barrier Function as a Target of Hyperthermia
Material and Methods. The isolated mitochondria from rat heart and liver (of both genders) were used for experiments, as well as mitochondria isolated from the perfused male rat liver. Changes in the membrane permeability were evaluated by mitochondrial respiration in state 2 or by estimation of the modular kinetics of the membrane leak.
Results. The inner membrane of isolated mitochondria from healthy tissues was found to be an extremely sensitive target of hyperthermia that exerted the response even in the febrile range. More severe hyperthermia compromised the inner mitochondrial membrane function; however, this response was tissue-specific and, to some extent, gender-dependent (for liver mitochondria). The data obtained by direct heating of isolated mitochondria were validated by experiments on the perfused liver.
Conclusions. The obtained results imply a crucial importance of the evaluation of the tissue- and gender-specific differences while developing or improving the protocols for hyperthermic treatment or combinatory therapy.
Naučienė, Z.; Žūkienė, R.; Degutytė-Fomins, L.; Mildažienė, V. Mitochondrial Membrane Barrier Function as a Target of Hyperthermia. Medicina 2012, 48, 36.
Naučienė Z, Žūkienė R, Degutytė-Fomins L, Mildažienė V. Mitochondrial Membrane Barrier Function as a Target of Hyperthermia. Medicina. 2012; 48(5):36.Chicago/Turabian Style
Naučienė, Zita; Žūkienė, Rasa; Degutytė-Fomins, Laima; Mildažienė, Vida. 2012. "Mitochondrial Membrane Barrier Function as a Target of Hyperthermia." Medicina 48, no. 5: 36.