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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Effect of (1→3),(1→6)-β-Glucan on In Vitro Production of Cytokines by Leukocytes of Patients With Periodontitis

1
Department of Microbiology, Medical Academy, Lithuanian University of Health Sciences
2
Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences
3
Department of Dental and Maxillofacial Orthopedics, Medical Academy, Lithuanian University of Health Sciences
4
Department of Maxillofacial Surgery, Medical Academy, Lithuanian University of Health Sciences
*
Author to whom correspondence should be addressed.
Medicina 2012, 48(4), 27; https://doi.org/10.3390/medicina48040027
Received: 27 February 2012 / Accepted: 30 April 2012 / Published: 5 May 2012
Background. The aim of the study was to investigate the effect of (1→3), (1→6)-β-glucan on the production of interleukin 10 (IL-10) and tumor necrosis factor α (TNF-α) in vitro by peripheral blood leukocytes of patients with periodontitis.
Material and Methods
. In total, 20 patients suffering from untreated severe chronic generalized periodontitis were enrolled in this study. Periodontitis was confirmed by clinical and radiologic examination. Besides, 20 periodontally healthy patients served as a control group. Peripheral venous blood was sampled from the patients, and isolated leukocytes were treated with (1→3),(1→6)-β-glucan from yeast at different concentrations. The levels of IL-10 and TNF-α secreted by the leukocytes unstimulated and stimulated with unopsonized E. coli in vitro were determined by the enzyme amplified sensitivity immunoassay method.
Results.
Our data showed that (1→3),(1→6)-β-glucan induced a significant decrease (P<0.05) in the TNF-α level and a significant increase (P<0.001) in the IL-10 level in the media of unstimulated and stimulated leukocytes of the patients with periodontitis in comparison with those of the healthy subjects.
Conclusions
. The present in vitro study showed that (1→3),(1→6)-β-glucan modulated the response of leukocytes of the patients with periodontitis differently in comparison with those of the healthy subjects. It increased the release of IL-10, which is protective of the tooth-supporting tissues in patients with periodontal disease, but decreased the release of TNF-α, which is mainly responsible for the destruction of the tooth-supporting tissues during periodontal disease.
Keywords: periodontal disease; (1→3),(1→6)-β-glucan; interleukin 10; tumor necrosis factor α periodontal disease; (1→3),(1→6)-β-glucan; interleukin 10; tumor necrosis factor α
MDPI and ACS Style

Akramas, L.; Akramienė, D.; Sakalauskienė, J.; Kubilius, R.; Gleiznys, A. Effect of (1→3),(1→6)-β-Glucan on In Vitro Production of Cytokines by Leukocytes of Patients With Periodontitis. Medicina 2012, 48, 27.

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