Recovery of Infarcted Myocardium in an In Vivo Experiment
Material and Methods. As a model for myocardium restoration in vivo, experimental rabbit heart infarct was used. Autologic adult myogenic stem cells were isolated from skeletal muscle and propagated in culture. Before transplantation, the cells were labeled with 4´,6-diamidino-2-phenylindole and then, during heart surgery, introduced into the rabbit acutely infarcted myocardium. Postoperative cardiac function was monitored by recording electrocardiograms and echocardiograms. At the end of the experiment, the efficiency of cell integration was evaluated histologically.
Results. Rabbit cardiac function recovered after 1 month after the induction of experimental infarction both in the control and experimental groups. Therefore, the first month after the infarction was the most significant for the assessment of cell transplantation efficacy. Transplanted cell integration into infarcted myocardium was time- and individual-dependent. Evaluation of changes in left ventricular ejection fraction after the induction of myocardial infarction revealed better recovery in the experimental group; however, the difference among animals in the experimental and control groups varied and was not significant.
Conclusions. Autologous myogenic stem cells repopulated infarcted myocardium with different efficiency in each individual. This variability may account for the observed difference in postoperative cardiac recovery in a rabbit model.
Širmenis, R.; Kraniauskas, A.; Jarašienė, R.; Baltriukienė, D.; Kalvelytė, A.; Bukelskienė, V. Recovery of Infarcted Myocardium in an In Vivo Experiment. Medicina 2011, 47, 88.
Širmenis R, Kraniauskas A, Jarašienė R, Baltriukienė D, Kalvelytė A, Bukelskienė V. Recovery of Infarcted Myocardium in an In Vivo Experiment. Medicina. 2011; 47(11):88.Chicago/Turabian Style
Širmenis, Raimondas; Kraniauskas, Antanas; Jarašienė, Rasa; Baltriukienė, Daiva; Kalvelytė, Audronė; Bukelskienė, Virginija. 2011. "Recovery of Infarcted Myocardium in an In Vivo Experiment." Medicina 47, no. 11: 88.