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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
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An In Vitro and In Vivo Study on the Intensity of Adhesion and Colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on Originally Synthesized Biomaterials With Different Chemical Composition and Modified Surfaces and Their Effect on Expression of TNF-α, β-Defensin 2 and IL-10 in Tissues

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Department of Biology and Microbiology, Riga Stradins University
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Institute of Anatomy and Anthropology, Riga Stradins University
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Centre of Development and Innovation of Biomaterials, Riga Technical University
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Institute of Stomatology, Riga Stradins University
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Faculty of Medicine, Riga Stradins University, Latvia
*
Author to whom correspondence should be addressed.
Medicina 2011, 47(10), 80; https://doi.org/10.3390/medicina47100080
Received: 22 August 2011 / Accepted: 31 October 2011 / Published: 5 November 2011
The aim of this study was to determine adhesion and colonization of bacteria on the surface of originally synthesized glass-ceramic biomaterials and their effect on inflammation reactions in tissues surrounding the implant.
Materials and Methods.
Biomaterial discs were contaminated with bacterial suspensions of 10, 102, and 103 colony forming units (CFU)/mL (P. aeruginosa ATCC 27853 and S. epidermidis ATCC 12228), and after 2 hours of cultivation, the intensity of bacterial adhesion was determined. For in vivo tests, the samples were contaminated with 102 and 103 CFU/mL cultivated at 37oC for 2 h to ensure bacterial adhesion. Contaminated biomaterial samples were implanted in the interscapular area of chinchilla rabbits for 2 and 4 weeks. The biomaterials were removed, and using plate count and sonification methods, bacterial colonization on the surface of biomaterials was determined. Moreover, the expression of TNF-α, β-defensin 2, and IL-10 in the surrounding tissues was assessed by using immunohistochemistry methods.
Results. P. aeruginosa more intensively colonized biomaterials in the in vivo study as compared with S. epidermidis. Il-10 is a regulatory cytokine, which reduces the intensity of inflammatory cell activity, thus reducing nonspecific resistance of the organism.
Conclusions
. The expression of TNF-α and IL-10 was not affected by short (2 and 4 weeks) biomaterial implantation. Pronounced cytokine expression in tissues around implanted biomaterials contaminated with P. aeruginosa was observed.
Keywords: biomaterials; Staphylococcus epidermidis; Pseudomonas aeruginosa; interleukin 10; tumor necrosis factor α; β-defensin 2 biomaterials; Staphylococcus epidermidis; Pseudomonas aeruginosa; interleukin 10; tumor necrosis factor α; β-defensin 2
MDPI and ACS Style

Reinis, A.; Pilmane, M.; Stunda, A.; Vētra, J.; Kroiča, J.; Rostoka, D.; Šalms, Ģ.; Vostroilovs, A.; Dons, A.; Bērziņa-Cimdiņa, L. An In Vitro and In Vivo Study on the Intensity of Adhesion and Colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on Originally Synthesized Biomaterials With Different Chemical Composition and Modified Surfaces and Their Effect on Expression of TNF-α, β-Defensin 2 and IL-10 in Tissues. Medicina 2011, 47, 80.

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