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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

mTHPC-mediated photodynamic treatment of Lewis lung carcinoma in vitro and in vivo

1
Department of Biochemistry and Biophysics, Vilnius University
2
Institute of Oncology, Vilnius University, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2010, 46(5), 345; https://doi.org/10.3390/medicina46050049
Received: 10 February 2009 / Accepted: 7 May 2010 / Published: 12 May 2010
Background and objective. The ongoing search for the enhancement of efficacy of photodynamic therapy stimulates the interest in molecular mechanisms of the response to the treatment. Looking for the cell line suitable for investigation of cellular response both in vivo and in vitro, we evaluated phototoxicity of m-tetrakis-(3-hydroxyphenyl)-chlorin (mTHPC) on viability of Lewis lung carcinoma (LLC1) cells in vitro, growth of murine transplantable tumor, and mice survival.
Material and methods
. LLC1 cell culture and male C57BL/6 mice bearing Lewis lung carcinoma were used for the experiments. Photodynamic treatment was mediated by m-tetrakis-(3- hydroxyphenyl)-chlorin as a photosensitizer. Light emitting diode array was used for illumination. The effect of the photodynamic treatment was evaluated by comparison of viability of control and treated cells, growth of tumors, and survival of the control and treated mice.
Results
. In vitro, a cytotoxic dose inducing a reduction in viability of LLC1 cells by 50% was achieved at 60 mJ/cm2 and approximately 400 ng/mL of the photosensitizer, or 30 mJ/cm2 and 600 ng/mL of mTHPC. Both the concentration of the photosensitizer and duration of light exposure were significant determinants of cytotoxic effect. In vivo, an injection of 0.25 mg/kg of mTHPC to mice bearing Lewis lung tumor and illumination at 120 J/cm2 taking place after 24 h significantly inhibited tumor growth and prolonged mice survival. However, the tumors regained their growth potential after 9 days.
Conclusions
. Photodynamic treatment mediated by m-tetrakis-(3-hydroxyphenyl)-chlorin had a significant effect on LLC1 cells in vitro and growth of Lewis lung carcinoma in vivo.
Keywords: photodynamic treatment; LLC1 cell line; Lewis lung carcinoma; photosensitizer; mTHPC photodynamic treatment; LLC1 cell line; Lewis lung carcinoma; photosensitizer; mTHPC
MDPI and ACS Style

Dabkevičienė, D.; Stankevičius, V.; Graželienė, G.; Markuckas, A.; Didžiapetrienė, J.; Kirvelienė, V. mTHPC-mediated photodynamic treatment of Lewis lung carcinoma in vitro and in vivo. Medicina 2010, 46, 345.

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