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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Anaphylaxis of small arteries: putative role of nitric oxide and prostanoids

Department of Physiology, Kaunas University of Medicine, Lithuania
Author to whom correspondence should be addressed.
Medicina 2010, 46(1), 38;
Received: 30 March 2009 / Accepted: 4 January 2010 / Published: 9 January 2010
This study investigated possible implication of nitric oxide and prostanoids in anaphylactic reaction in small mesenteric and coronary arteries.
Material and methods. Isolated arteries from guinea pigs, sensitized with 0.5 mL of horse serum or sham-sensitized, were challenged with 1% of horse serum in vitro. Contractile responses of arteries (normalized diameter, 350–450 μm) were recorded by a small blood vessel wire myograph. For inhibition of the release of NO or prostanoids, vessels were pretreated with N(G)-nitro-L-arginine methyl ester (30 μM) or indomethacin (10 μM), respectively.
Results. Antigen challenge was followed by contraction of both coronary and mesenteric vessels. Two patterns of contraction were observed: 1) peak contraction – an immediate transient contraction of relatively high amplitude; this was the most common pattern; 2) biphasic: the initial peak contraction was followed by a slow growing contraction with low amplitude. Biphasic pattern was observed in 60% of the mesenteric vessels and 40% of the coronary vessels. Inhibition of NO synthase significantly increased the peak contraction in the coronary vessels and the second-phase contraction in the mesenteric vessels. Inhibition of cyclooxygenase caused a decrease in the peak and second-phase contraction of both the coronary and mesenteric vessels.
Conclusions. Despite anaphylactic contraction, nitric oxide seems to be released from the endothelium following antigen challenge in the small coronary and mesenteric arteries. This may contribute to the development of hypotension during anaphylaxis. Prostanoids are playing a different role – the contracting products of cyclooxygenase pathway are important for the development of anaphylactic contraction of the small isolated arteries.
Keywords: anaphylaxis; guinea pig; endothelial relaxing factors; nitric oxide; indomethacin anaphylaxis; guinea pig; endothelial relaxing factors; nitric oxide; indomethacin
MDPI and ACS Style

Laukevičienė, A.; Uginčius, P.; Korotkich, I.; Lažauskas, R.; Kėvelaitis, E. Anaphylaxis of small arteries: putative role of nitric oxide and prostanoids. Medicina 2010, 46, 38.

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