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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Analysis of content of phenolic acids in Lithuanian propolis using high-performance liquid chromatography technique

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Department of Drug Technology and Social Pharmacy
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Department of Analytical and Toxicological Chemistry, Kaunas University of Medicine
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Department of Plant Protection, Lithuanian University of Agriculture, Lithuaniaa
*
Author to whom correspondence should be addressed.
Medicina 2009, 45(9), 712; https://doi.org/10.3390/medicina45090093
Received: 13 December 2007 / Accepted: 4 September 2009 / Published: 9 September 2009
The aim of the study was to analyze phenolic acids in Lithuanian propolis and to compare it with the composition of propolis in neighboring countries (Latvia and Poland) according to the predominant flora in the collecting places. The study was also aimed at the evaluation of the effect of the layer thickness (mm) of the harvested propolis on the quality of the raw material in determining the amount of phenolic acids.
Materials and methods.
The object of the study was propolis collected in Lithuania, Poland, and Latvia in late July of 2006 and 2007. The qualitative and quantitative analysis of phenolic acids was performed using the high-performance liquid chromatography technique (HPLC).
Results. The results of the study showed that the quantitative and qualitative composition of phenolic acids in propolis depended on the plants from which the bees in the area collected substances for the raw material of propolis. The predominant phenolic acids were determined to be ferulic and coumaric acids, and they may be among the main indicators of quality in the standardization of the raw material and preparations of propolis.
Conclusion
. We created an HPLC-based analysis method for the identification and quantification of phenolic acids in propolis. The variety of phenolic acids in propolis depends on the vegetation predominating in the harvesting area. Studies have shown that the highest amount of phenolic acids is observed in propolis harvested in areas characterized by the predominance of deciduous trees and meadows. Results have also shown that ferulic and coumaric acids are the predominant phenolic acids in propolis. The thickness of the layer of the collected propolis in the hive also influences its chemical composition.
Keywords: propolis; high-performance liquid chromatography; phytochemical compounds; phenolic acids propolis; high-performance liquid chromatography; phytochemical compounds; phenolic acids
MDPI and ACS Style

Ramanauskienė, K.; Savickas, A.; Inkėnienė, A.; Vitkevičius, K.; Kasparavičienė, G.; Briedis, V.; Amšiejus, A. Analysis of content of phenolic acids in Lithuanian propolis using high-performance liquid chromatography technique. Medicina 2009, 45, 712.

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