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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Effect of anoxia and Polyscias filicifolia Bailey biomass tincture on the activity of tRNA and aminoacyl-tRNA synthetases in isolated pig heart

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Department of Biochemistry
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Department of Drug Chemistry
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Institute for Biomedical Research
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Department of Physiology, Kaunas University of Medicine, Lithuania
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Author to whom correspondence should be addressed.
Medicina 2009, 45(6), 486; https://doi.org/10.3390/medicina45060064
Received: 8 July 2008 / Accepted: 4 June 2009 / Published: 9 June 2009
Objective. The aim of this study was to investigate effect of anoxia and Polyscias filicifolia Bailey biomass tincture on the activities of different tRNA and aminoacyl-tRNA synthetases in isolated pig heart.
Material and methods. The isolated pig heart was perfused according to the modified method of Langendorf, using an artificial blood circulation apparatus. Anoxia 20 min in duration was performed by perfusion of isolated heart with Krebs-Henseleit bicarbonate buffer saturated with gas mixture (95% N2 and 5% CO2). Control heart was perfused with the same buffer saturated with gas mixture (95% O2 and 5% CO2). Effect of Polyscias filicifolia Bailey biomass tincture was evaluated by perfusion of isolated heart with a buffer containing tincture. Total tRNA and aminoacyl-tRNA synthetases were isolated from pig heart. Activities of tRNA and aminoacyltRNA synthetases were measured by the aminoacylation reaction using C14-amino acids.
Results
. Anoxia 20 min in duration has caused a decrease in the acceptor activity of tRNA and increase in the activities of aminacyl-tRNA synthetases. Polyscias filicifolia Bailey tincture did not affect the acceptor activity of tRNA and activities aminacyl-tRNA synthetases. After 20-min anoxic perfusion with the buffer containing Polyscias filicifolia Bailey biomass tincture, the acceptor activities of tRNA increased to the control value and activities of aminacyl-tRNA synthetases reached the control value.
Conclusions
. The acceptor activity of tRNA from isolated pig heart decreased and activities of aminacyl-tRNA synthetases increased under anoxia. Perfusion with buffer containing tincture of Polyscias filicifolia Bailey biomass restored acceptor activities of tRNA and activities of aminacyl-tRNA synthetases.
Keywords: anoxia; pig heart; Polyscias filicifolia Bailey; tRNA; aminoacyl-tRNA synthetase anoxia; pig heart; Polyscias filicifolia Bailey; tRNA; aminoacyl-tRNA synthetase
MDPI and ACS Style

Kašauskas, A.; Rodovičius, H.; Vieželienė, D.; Lažauskas, R. Effect of anoxia and Polyscias filicifolia Bailey biomass tincture on the activity of tRNA and aminoacyl-tRNA synthetases in isolated pig heart. Medicina 2009, 45, 486.

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