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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Treatment of Lewis lung carcinoma by photodynamic therapy and glucan from barley

1
Department of Physiology, Kaunas University of Medicine
2
Institute of Oncology, Vilnius University, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2009, 45(6), 480; https://doi.org/10.3390/medicina45060063
Received: 22 May 2009 / Accepted: 3 June 2009 / Published: 8 June 2009
Objective. During the photodynamic treatment, complement system is activated and tumor cells are opsonized with iC3b fragment. β-glucans can enhance cytotoxicity of iC3bopsonized cells due to their specific interaction with complement receptor 3 (CR3; CD11b/CD18) on the surface of the effector cells. In contrast to microorganisms, tumor cells lack β-glucan as a surface component and cannot trigger complement receptor 3-dependent cellular cytotoxicity and initiate tumor-killing activity. This mechanism could be induced in the presence of β-glucans. This study aimed at determining the influence of coadministration of β-glucan from barley on the efficacy of photodynamic tumor therapy (PDT).
Material and methods
. C57 Bl/6 female mice bearing Lewis lung carcinoma were used throughout the study. Mice were randomized into groups (15 in each group) and exposed to the treatment with intravenous Photofrin injection (dose, 10 mg/kg) and after 24 h following laser illumination, or with oral administration of β-glucan from barley at a dose of 400 μg/mouse per day up to 5 days, or with their combination. Tumor growth dynamics and survival of the treated and untreated mice were monitored.
Results
. Tumor volume in all treated groups was significantly lower (P<0.001) than that in the control group. The most effective tumor growth suppression (P=0.033) was achieved in mice treated with combination of PDT and β-glucan from barley as compared with PDT alone. The best survival was achieved in the same group, but difference was not significant as compared to the control group (P=0.143) and to PDT alone group (P=0.319).
Conclusions. The present study demonstrates that coadministration of β-glucan from barley can enhance efficacy of photodynamic therapy.
Keywords: β-glucan; complement receptor; photodynamic tumor therapy; Lewis lung carcinoma β-glucan; complement receptor; photodynamic tumor therapy; Lewis lung carcinoma
MDPI and ACS Style

Akramienė, D.; Graželienė, G.; Didžiapetrienė, J.; Kėvelaitis, E. Treatment of Lewis lung carcinoma by photodynamic therapy and glucan from barley. Medicina 2009, 45, 480.

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