Next Article in Journal
Associations between schoolchildren’s educational load and health, and the day’s routine
Previous Article in Journal
Changes of vision and refraction in patients with thyroid pathology
Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Investigation of contribution of individual constituents to antioxidant activity in herbal drugs using postcolumn HPLC method

1
Department of Pharmacognosy
2
Department of Pharmaceutical Chemistry, Kaunas University of Medicine, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2009, 45(5), 382; https://doi.org/10.3390/medicina45050049
Received: 8 May 2008 / Accepted: 4 May 2009 / Published: 9 May 2009
The most important attention is paid to the search of natural antioxidants and their evaluation in medicinal and food raw materials of plant origin. A number of plants, their extracts, food products, and medicinal preparations appear to be the objects of scientific research. Effectiveness and informative character of research, undoubtedly, depend on relevance, sensitivity, and efficiency of the methods chosen. The aim of this work was to develop and validate the postcolumn high-performance liquid chromatography (HPLC)-DPPH method as well as its application in the evaluation of antioxidant activity of known and unknown compounds scavenging free radicals and existing in medicinal plant raw materials. HPLC-separated compounds were identified at the wavelength of 275 nm, and then the mobile phase with analytes flowed through a mixing tee to the reaction coil, where DPPH reagent solution was supplied. The solution flow rate was 0.4 mL/min. The reaction coil was connected with UV/VIS type detector, which measured absorption of flowing solution at the wavelength of 520 nm. It was determined that vitexin rhamnoside, the dominant compound in the leaves of Crataegus monogyna, was not a significant radical scavenger. The most active antioxidant in the leaves and flowers of Crataegus monogyna was chlorogenic acid. The most active antioxidant in Origanum vulgare raw material was rosmarinic acid. Identified analytes in the extracts of Achillea millefolium that possessed radical-scavenging properties were chlorogenic acid, luteolin-7-Oglucoside, rutin, and luteolin.
Keywords: antioxidants; postcolumn high-performance liquid chromatography; DPPH radical; herbal drug antioxidants; postcolumn high-performance liquid chromatography; DPPH radical; herbal drug
MDPI and ACS Style

Raudonis, R.; Jakštas, V.; Burdulis, D.; Benetis, R.; Janulis, V. Investigation of contribution of individual constituents to antioxidant activity in herbal drugs using postcolumn HPLC method. Medicina 2009, 45, 382.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop