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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

1
Scientific Research Center, Institute of Oncology, Vilnius University
2
Department of Biology, Vytautas Magnus University
3
Department of Biochemistry and Biophysics, Vilnius University, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2009, 45(5), 372; https://doi.org/10.3390/medicina45050047
Received: 17 November 2008 / Accepted: 4 May 2009 / Published: 9 May 2009
The aim of our study was to determine if electroporation could improve the efficacy of photodynamic tumor therapy. A disadvantage of photodynamic therapy is a slow and in some cases insufficient accumulation of photosensitizer in tumor tissue, which could restrict the achievement of an efficient dose. Under the action of electric pulses, cells undergo membrane electroporation, which results in an increased permeability to various exogenous molecules. In this study, murine hepatoma MH22A cells were exposed to light in vitro in the presence of a photosensitizer, either chlorin e6 or aluminum phthalocyanine tetrasulfonate, following electroporation. Accumulation of the photosensitizers was registered by fluorescence microscopy. Cell viability was determined by the MTT assay. Our results demonstrate that electroporation improves an access of chlorin e6 and aluminum phthalocyanine tetrasulfonate to MH22A cells. Electroporation in combination with photosensitization significantly reduces viability of the treated cells even at low doses of photosensitizers.
Keywords: electroporation; photodynamic tumor therapy; chlorin e6; aluminum phthalocyanine tetrasulfonate electroporation; photodynamic tumor therapy; chlorin e6; aluminum phthalocyanine tetrasulfonate
MDPI and ACS Style

Labanauskienė, J.; Šatkauskas, S.; Kirvelienė, V.; Venslauskas, M.; Atkočius, V.; Didžiapetrienė, J. Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro. Medicina 2009, 45, 372.

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