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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Histology of human glioblastoma transplanted on chicken chorioallantoic membrane

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Department of Neurosurgery
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Institute for Biomedical Research
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Department of Histology and Embryology
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Department of Physics, Mathematics, and Biophysics
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Department of Pathological Anatomy, Kaunas University of Medicine, Lithuania
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Institute of Neuropathology, RWTH Aachen University Clinics, Germany
*
Author to whom correspondence should be addressed.
Medicina 2009, 45(2), 123; https://doi.org/10.3390/medicina45020016
Received: 8 April 2008 / Accepted: 5 February 2009 / Published: 10 February 2009
Glioblastoma is the most malignant tumor in the range of cerebral astrocytic gliomas. A lot of experimental models are used to evaluate various properties of glioblastoma. Chicken chorioallantoic membrane model is one of them.
Objective. To evaluate histology and survival of glioblastoma tumors taken immediately from operating theatre and transplanted on chicken chorioallantoic membrane.
Materials and methods. Glioblastoma samples obtained from 10 patients were transplanted onto 200 eggs. Overall, we used 15 tumors; only 5 of them were not glioblastomas as it was revealed later.
Results
. The transplanted tumors survive up to 6 days. Transplants do not survive longer because during embryo’s development the nourishing membrane dries. Transplanted glioblastomas exhibited the same features as original glioblastomas – necrosis, endothelium proliferation, cellular polymorphism – while transplanted glioblastomas also showed glial fibrillary acidic protein (GFAP), vimentin, Ki67, S100 protein, neurofilament immunoreactivity, and infiltration of macrophages (CD68) and T cells (CD3+, CD8+). Transplanted glioblastomas did not show any immunoreactivity of p53. Invasion of vessels from the chicken into transplanted tumor is not observed. Chicken erythrocytes did not appear within the transplants, and tumor cells invade chicken tissue at the minimum.
Conclusion. Our data show that transplanted pieces of glioblastoma survive with all cytological features. The presence of macrophages (marker CD68) and T cells (markers CD3+ and CD8+) can be registered in the transplant. The data revealed that transplanted glioblastoma remains as insulated unit, which survives from nourishment of the chorioallantoic membrane apparently only by diffusion. The features of original tumor-host reaction of the patient remained too.
Keywords: glioblastoma; chicken embryo; chorioallantoic membrane; immunohistochemistry; inflammatory cells glioblastoma; chicken embryo; chorioallantoic membrane; immunohistochemistry; inflammatory cells
MDPI and ACS Style

Balčiūnienė, N.; Tamašauskas, A.; Valančiūtė, A.; Deltuva, V.; Vaitiekaitis, G.; Gudinavičienė, I.; Weis, J.; Von Keyserlingk, D.G. Histology of human glioblastoma transplanted on chicken chorioallantoic membrane. Medicina 2009, 45, 123.

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