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Helicobacter pylori genotypes in Lithuanian patients with chronic gastritis and duodenal ulcer
by
Jolanta Miciulevičienė 1, Henrikas Čalkauskas 2, Laimas Jonaitis 1, Gediminas Kiudelis 1, Vytas Tamošiūnas 3, Antanas Praškevičius 4, 
Limas Kupčinskas 1,* and Duglas Berg 5
Limas Kupčinskas 1,* and Duglas Berg 5
1
Department of Gastroenterology, Kaunas University of Medicine
2
Medical Center “Euroclinics”
3
Department of Zoology, Vilnius Pedagogical University
4
Department of Biochemistry, Kaunas University of Medicine, Lithuania
5
Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri, USA
*
Author to whom correspondence should be addressed.
Medicina 2008, 44(6), 449; https://doi.org/10.3390/medicina44060058
Received: 14 April 2008 / Accepted: 12 June 2008 / Published: 17 June 2008
Objective. Clinical outcome of Helicobacter pylori (H. pylori) infection might be associated with specific virulence-associated bacterial genotypes. The distribution of different bacterial genotypes varies geographically. The aim of this study was to assess the relationship between cagPAI, vacA, and iceA status and severity of the disease in patients from Lithuania, infected by H. pylori. Material and methods. H. pylori from 81 patients (37 with duodenal ulcer and 44 with chronic gastritis) was isolated from gastric biopsy specimens and cultured. Bacterial genotypes cagPAI, vacA (s and m subtypes) and iceA were analyzed by polymerase chain reaction using specific primers.
Results. The cagPAI was identified in 59.3% of Lithuanian H. pylori strains investigated. H. pylori strains cultured from duodenal ulcer (DU) patients more frequently (P<0.01) contained cagPAI and vacA s1 genotypes (75.7% and 75.7%, respectively) in comparison to isolates from chronic gastritis (CG) patients (45.5% and 40.9%, respectively). Evaluation of nucleotide sequence of the vacA middle-region revealed that vacA s2/m2 genotype was more frequent in CG than in DU patients (56.8% and 24.3%, respectively; P<0.05). We have not found any differences in the frequency of iceA1 genotype between the DU and CG patients (46.0% and 40.9%, respectively; P>0.05).
Conclusion. Our study suggests that cagPAI and vacA s1 genotypes are associated with peptic ulceration in Lithuanian patients infected by H. pylori.
Results. The cagPAI was identified in 59.3% of Lithuanian H. pylori strains investigated. H. pylori strains cultured from duodenal ulcer (DU) patients more frequently (P<0.01) contained cagPAI and vacA s1 genotypes (75.7% and 75.7%, respectively) in comparison to isolates from chronic gastritis (CG) patients (45.5% and 40.9%, respectively). Evaluation of nucleotide sequence of the vacA middle-region revealed that vacA s2/m2 genotype was more frequent in CG than in DU patients (56.8% and 24.3%, respectively; P<0.05). We have not found any differences in the frequency of iceA1 genotype between the DU and CG patients (46.0% and 40.9%, respectively; P>0.05).
Conclusion. Our study suggests that cagPAI and vacA s1 genotypes are associated with peptic ulceration in Lithuanian patients infected by H. pylori.
Keywords:
Helicobacter pylori; duodenal ulcer; chronic gastritis; virulence factors; cagPAI; vacA; iceA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Miciulevičienė, J.; Čalkauskas, H.; Jonaitis, L.; Kiudelis, G.; Tamošiūnas, V.; Praškevičius, A.; Kupčinskas, L.; Berg, D. Helicobacter pylori genotypes in Lithuanian patients with chronic gastritis and duodenal ulcer. Medicina 2008, 44, 449.
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