Next Article in Journal
Expression of matrix metalloproteinases (MMP-2 and MMP-9) in vocal fold polyps
Previous Article in Journal
Sinoorbital gunshot injuries Endoscopic diagnostics and management
Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Tympanic membrane changes in experimental acute otitis media and myringotomy

1
Department of Otorhinolaryngology, Kaunas University of Medicine, Lithuania
2
Department of Otorhinolaryngology, Head and Neck Surgery, Lund University, Sweden
3
Department of Otorhinolaryngology, Head and Neck Surgery, Gentofte University Hospital of Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Medicina 2008, 44(4), 313; https://doi.org/10.3390/medicina44040041
Received: 14 February 2008 / Accepted: 15 April 2008 / Published: 20 April 2008
Objective. The present experimental study explored pathomorphological changes and calcium depositions in the tympanic membrane during experimental acute otitis media caused by nontypeable Haemophilus influenzae in myringotomized and nonmyringotomized ears.
Material and methods
. A rat model of experimental acute otitis media caused by nontypeable Haemophilus influenzae was employed. Sixteen Sprague-Dawley rats were used. Four days following middle ear inoculation, a bilateral myringotomy was performed in six randomly selected animals. Another group of 10 animals was inoculated only. On days 4, 7, 14, and 28 after inoculation, two animals from each group were sacrificed. The temporal bones were removed and the tympanic membranes were dissected, followed by paraffin embedding. Adjacent sections were stained with PAS-alcian blue for basic histopathological observations and by von Kossa method for determination of calcium phosphate depositions.
Results. Particularly intense invasion of polymorphonuclear neutrophil leukocytes was seen on day 4 after inoculation. The highest infiltration of macrophages was observed on day 7. The peak number of lymphocytes was seen on day 14. No difference occurred in the number of polymorphonuclear leukocytes in myringotomized and nonmyringotomized tympanic membranes. The infiltration with lymphocytes and activated macrophages in all parts of the myringotomized tympanic membranes was statistically significantly higher than in the nonmyringotomized animals. The total amount of interstitial calcium phosphate depositions during days 7, 14, and 28 of study was statistically higher in the sections of pars tensa from myringotomized membranes compared to the nonmyringotomized membranes.
Conclusion
. Nontypeable Haemophilus influenzae-induced acute otitis media and myringotomy provoke more extensive inflammatory reaction with microcalcification in the tympanic membranes.
Keywords: acute otitis media; myringotomy; myringosclerosis; animal model acute otitis media; myringotomy; myringosclerosis; animal model
MDPI and ACS Style

Alzbutienė, G.; Hermansson, A.; Cayè-Thomasen, P.; Kinduris, V. Tympanic membrane changes in experimental acute otitis media and myringotomy. Medicina 2008, 44, 313.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop