Thickness of cerebral cortex measured using anatomical mesoscopic imaging and magnetic resonance imaging
Material and methods. The thickness of cerebral cortex was measured in symmetrical Brodmann’s areas of both hemispheres. The anatomical mesoscopic imaging technique was used for the examination of 2×2-cm cortex samples obtained during autopsy and fixed for 4 weeks in 10% paraformaldehyde. In these samples, cortex thickness was measured in sections perpendicular to the convolution, using an operative microscope, in a mesoscopic image at ×16 magnification and with an accuracy of 0.01 mm. Using cerebral magnetic resonance imaging, the thickness of cerebral cortex in live subjects was measured on T1-weighted images of patients examined at the Clinic of Radiology, Kaunas University of Medicine Hospital. The measured cortical field image was magnified to the smallest element of digital image – the pixel – and measured with an accuracy of 0.01 mm. Each of the two techniques was applied for the examination of 20 men and women who were divided into age groups of 20–60 years (n=10) and older than 60 years (n=10).
Results and conclusions. Both examination methods yielded a statistically significant difference in the thickness of cerebral cortex between Brodmann’s areas 1, 4, and 19. No significant difference in cortex thickness was found between different age and sex groups; however, the findings showed that the difference in cortex thickness between the different age male groups was 4.6% and female – 1.6%. No significant difference using different techniques was found, but the cortex thickness in the fixed samples was reduced by 0.5 cm on average.
Janauskaitë, L.; Kaèerauskienë, J.; Jaðinskaitë, U.; Gedrimas, V.; Stropus, R. Thickness of cerebral cortex measured using anatomical mesoscopic imaging and magnetic resonance imaging. Medicina 2008, 44, 126.
Janauskaitë L, Kaèerauskienë J, Jaðinskaitë U, Gedrimas V, Stropus R. Thickness of cerebral cortex measured using anatomical mesoscopic imaging and magnetic resonance imaging. Medicina. 2008; 44(2):126.Chicago/Turabian Style
Janauskaitë, Liuda; Kaèerauskienë, Justina; Jaðinskaitë, Ugnë; Gedrimas, Vytautas; Stropus, Rimvydas. 2008. "Thickness of cerebral cortex measured using anatomical mesoscopic imaging and magnetic resonance imaging." Medicina 44, no. 2: 126.