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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Evaluation of phenolic acids and phenylpropanoids in the crude drugs

Department of Analytical and Toxicological Chemistry
Department of Pharmaceutical Chemistry and Pharmacognosy, Kaunas University of Medicine
Laboratory of Economical Botany, Institute of Botany, Vilnius
Department of Pharmaceutical Technology and Social Pharmacy, Kaunas University of Medicine, Lithuania
Author to whom correspondence should be addressed.
Medicina 2008, 44(1), 48;
Received: 21 November 2007 / Accepted: 5 December 2007 / Published: 10 December 2007
Phenolic acids and phenylpropanoids have an important biological activity and are therapeutic agents of crude drugs. Development of validated analysis techniques of these phytotherapeutic agents (fingerprinting and assay procedures) is an important practice for efficacy, safety, and quality control of herbal drug preparations. The aim of the present work was to study analytical capabilities of the evaluation of selected phenolic acids and phenylpropanoids: caffeic acid, chlorogenic acid, cinnamic acid, coumaric acid, ferulic acid, gallic acid, protocatechuic (3,4-dihydroxybenzoic) acid, rosmarinic acid, vanillic acid, and vanillin. Optimization and validation procedures of rapid and simple method of reversed-phase high-performance liquid chromatography were carried out. The mobile phase of the optimized chromatographic method consisted of methanol and 0.5% acetic acid solvent in water. For the application of method, two kinds of raw materials were chosen: propolis and the Herba Origani. Coumaric acid is the dominating phenolic acid of propolis (2785 mg/g). Results of analysis of Herba Origani demonstrated high quantities (6376 mg/g) of rosmarinic and protocatechuic (1485 mg/g) acids in the samples.
Keywords: phenolic acids; phenylpropanoids; high-performance liquid chromatography; crude drug phenolic acids; phenylpropanoids; high-performance liquid chromatography; crude drug
MDPI and ACS Style

Ivanauskas, L.; Jakštas, V.; Radušienė, J.; Lukošius, A.; Baranauskas, A. Evaluation of phenolic acids and phenylpropanoids in the crude drugs. Medicina 2008, 44, 48.

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