Next Article in Journal
Heart rate and systolic blood pressure response during the early exercise test and cardiovascular mortality after myocardial infarction
Previous Article in Journal
Surgical treatment of Graves’ disease: Subtotal thyroidectomy might still be the preferred option
Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Immune system alterations in patients with inflammatory bowel disease during remission

1
Department of Gastroenterology
2
Institute of Endocrinology, Kaunas University of Medicine
3
Laboratory of Immunology, Institute of Biotechnology
4
Department of Biochemistry, Kaunas University of Medicine
5
Department of Zoology, Vilnius Pedagogical University, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2008, 44(1), 27; https://doi.org/10.3390/medicina44010005
Received: 2 December 2007 / Accepted: 11 January 2008 / Published: 16 January 2008
Objective. Perturbed immune homeostasis elicited by misbalanced production of proinflammatory and anti-inflammatory cytokines is characteristic of inflammatory bowel disease. The aim of this study was to evaluate cytokine profile in patients with different forms of inflammatory bowel disease – ulcerative colitis and Crohn’s disease – during clinical remission phase.
Material and methods. Production of proinflammatory Th1 cytokines (tumor necrosis factoralpha (TNF-a), interferon-gamma (IFN-g)) and anti-inflammatory Th2 cytokines (interleukin- 10 (IL-10) and interleukin-13 (IL-13)) was analyzed in peripheral blood mononuclear cells of patients with inflammatory bowel disease (9 with ulcerative colitis and 9 with Crohn’s disease) and control subjects (n=11) by enzyme-linked immunosorbent assay (two-site ELISA).
Results
. The results of the study revealed that the level of TNF-a after stimulation with phytohemagglutinin in patients with Crohn’s disease was significantly higher in comparison to both patients with ulcerative colitis and controls (P<0.001 and P<0.01, respectively). The secretion of IFN-g both in patients with Crohn’s disease and ulcerative colitis was lower than that in controls (P=0.05 and P<0.01, respectively), but it normalized after stimulation with phytohemagglutinin. The levels of IL-10 and IL-13 were significantly (P<0.01) higher in patients with Crohn’s disease than in patients with ulcerative colitis and control group before and after stimulation with phytohemagglutinin.
Conclusions
. The results of our study provide evidence that in patients with inflammatory bowel disease, the imbalance between production of proinflammatory Th1 and anti-inflammatory Th2 cytokines persists even during remission of the disease, and disturbances of immune homeostasis are significantly more expressed in patients with Crohn’s disease than in patients with ulcerative colitis.
Keywords: Th1 and Th2 cytokines; inflammatory bowel disease; Crohn’s disease, ulcerative colitis Th1 and Th2 cytokines; inflammatory bowel disease; Crohn’s disease, ulcerative colitis
MDPI and ACS Style

Šventoraitytė, J.; Žvirblienė, A.; Kiudelis, G.; Žalinkevičius, R.; Žvirblienė, A.; Praškevičius, A.; Kupčinskas, L.; Tamošiūnas, V. Immune system alterations in patients with inflammatory bowel disease during remission. Medicina 2008, 44, 27.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop