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Medicina is published by MDPI from Volume 54 Issue 1 (2018). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Lithuanian Medical Association, Lithuanian University of Health Sciences, and Vilnius University.
Open AccessArticle

Study of diversity of anthocyanin composition in bilberry (Vaccinium myrtillus L.) fruits

1
Department of Pharmaceutical Chemistry and Pharmacognosy
2
Department of Analytical and Toxicological Chemistry, Kaunas University of Medicine
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Lithuanian Institute of Agriculture, Vokė branch, Lithuania
*
Author to whom correspondence should be addressed.
Medicina 2007, 43(12), 971; https://doi.org/10.3390/medicina43120127
Received: 30 October 2007 / Accepted: 20 November 2007 / Published: 25 November 2007
Qualitative and quantitative composition of anthocyanins in bilberry (Vaccinium myrtillus L.) fruits was assayed. The aim of our study was to evaluate total anthocyanin content and their composition in bilberries collected from various regions and at different time. For the quantification of total anthocyanins in frozen fruits, the spectrophotometrical assay was performed. The highest amount of anthocyanins in bilberry fruits, collected in Lithuania, was found in samples from Šilutė (0.399%), the lowest one – from Valkininkai region (0.264%), but higher amounts of anthocyanins were found in the samples collected in Russia (Archangelsk region) and Sweden (Stockholm region). High-performance liquid chromatography was applied for qualitative evaluation of individual anthocyanins in the different material. Quantification of anthocyanidin content was performed after acidic hydrolysis of anthocyanin glycosides. Chromatographic analysis has shown that there are no differences in qualitative composition of anthocyanidins. In all samples, cyanidin was found in the highest quantities (mean amount 0.053 µg/mL). Delphinidin and petunidin was found in quantities 2.5 fold lower than cyanidin, and malvidin and peonidin were found in the smallest quantities. Only in the blueberries collected in Sweden, malvidin was found in the highest amount. It was 1.5 fold higher than amounts of petunidin and delphinidin.
Keywords: blueberries; anthocyanins; anthocyanidins; spectrophotometry; high-performance liquid chromatography blueberries; anthocyanins; anthocyanidins; spectrophotometry; high-performance liquid chromatography
MDPI and ACS Style

Burdulis, D.; Ivanauskas, L.; Dirsė, V.; Kazlauskas, S.; Ražukas, A. Study of diversity of anthocyanin composition in bilberry (Vaccinium myrtillus L.) fruits. Medicina 2007, 43, 971.

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