Different Dose-Dependent Modes of Action of C-Type Natriuretic Peptide on Pseudomonas aeruginosa Biofilm Formation
Abstract
:1. Introduction
2. Results
2.1. Effect of CNP on P. aeruginosa Biofilm Formation in Dynamic Conditions
2.2. Involvement of AmiC in the CNP Effect on P. aeruginosa
2.3. Effect of CNP on P. aeruginosa Adhesion Properties
2.4. Effect of CNP on P. aeruginosa Di-Rhamnolipid Production
2.5. Effect of CNP on P. aeruginosa Membrane Fluidity
3. Discussion
4. Materials and Methods
4.1. Bacterial Cultures and Tested Molecules
4.2. Kinetics of Bacterial Growth
4.3. Biofilms Formation on Glass Slides Under Dynamic Conditions and Adhesion Analysis
4.4. Fluorescence Anisotropy Microplate Assays
4.5. Rhamnolipid Quantification
5. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Desriac, F.; Clamens, T.; Rosay, T.; Rodrigues, S.; Tahrioui, A.; Enault, J.; Roquigny, L.; Racine, P.-J.; Taupin, L.; Bazire, A.; et al. Different Dose-Dependent Modes of Action of C-Type Natriuretic Peptide on Pseudomonas aeruginosa Biofilm Formation. Pathogens 2018, 7, 47. https://doi.org/10.3390/pathogens7020047
Desriac F, Clamens T, Rosay T, Rodrigues S, Tahrioui A, Enault J, Roquigny L, Racine P-J, Taupin L, Bazire A, et al. Different Dose-Dependent Modes of Action of C-Type Natriuretic Peptide on Pseudomonas aeruginosa Biofilm Formation. Pathogens. 2018; 7(2):47. https://doi.org/10.3390/pathogens7020047
Chicago/Turabian StyleDesriac, Florie, Thomas Clamens, Thibaut Rosay, Sophie Rodrigues, Ali Tahrioui, Jérémy Enault, Lucille Roquigny, Pierre-Jean Racine, Laure Taupin, Alexis Bazire, and et al. 2018. "Different Dose-Dependent Modes of Action of C-Type Natriuretic Peptide on Pseudomonas aeruginosa Biofilm Formation" Pathogens 7, no. 2: 47. https://doi.org/10.3390/pathogens7020047