Heterophyllin: A New Adenia Toxic Lectin with Peculiar Biological Properties
Abstract
:1. Introduction
2. Results
2.1. Purification and Characterization of Adenia heterophylla Lectin
2.2. Enzymatic Activities of the Lectin from A. heterophylla Caudex
2.3. Immunological Properties of Heterophyllin
2.4. Biological Effects of Heterophyllin on Three Human-Derived Cell Lines
2.4.1. Heterophyllin Was Able to Completely Abolish Cell Viability
2.4.2. Heterophyllin Induced Apoptosis but Not Necrosis
2.4.3. Heterophyllin Cytotoxicity Was Strongly Reduced by ROS Scavengers and Cell Death Inhibitors
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Purification and Characterization of Adenia heterophylla Toxic Lectin
5.2. Heterophyllin Hemagglutinating and Enzymatic Activities
5.3. Heterophyllin Immunological Properties
5.4. Heterophyllin Cytotoxicity Mechanisms
5.5. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Purification Step | Protein (mg/mL) | Total Protein (mg) | Total Protein (%) | IC50 (µg/mL) 1 | Agglutinating Activity (µg/mL) 2 | Specific Activity (U/mg) 3 | Total Activity (U) | Yield (%) | |
---|---|---|---|---|---|---|---|---|---|
Non-Red. | Red. | ||||||||
Raw material | 2.17 | 650.2 | 100 | 42.2 | 9.15 | n.a. 4 | 109.3 | 71,051 | 100 |
Not retained | 1.39 | 583.0 | 89.7 | 117.0 | 112.0 | n.a. 4 | 8.9 | 5214 | 7.34 |
Purified protein | 0.173 | 5.622 | 0.86 | 2.11 | 0.142 | 43.25 | 7042 | 39,595 | 55.7 |
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Bortolotti, M.; Biscotti, F.; Zanello, A.; Polito, L.; Bolognesi, A. Heterophyllin: A New Adenia Toxic Lectin with Peculiar Biological Properties. Toxins 2024, 16, 1. https://doi.org/10.3390/toxins16010001
Bortolotti M, Biscotti F, Zanello A, Polito L, Bolognesi A. Heterophyllin: A New Adenia Toxic Lectin with Peculiar Biological Properties. Toxins. 2024; 16(1):1. https://doi.org/10.3390/toxins16010001
Chicago/Turabian StyleBortolotti, Massimo, Francesco Biscotti, Andrea Zanello, Letizia Polito, and Andrea Bolognesi. 2024. "Heterophyllin: A New Adenia Toxic Lectin with Peculiar Biological Properties" Toxins 16, no. 1: 1. https://doi.org/10.3390/toxins16010001