Matrix-assisted laser desorption/ionization (MALDI), a soft ionization method, coupling with time-of-flight mass spectrometry (TOF MS) has become an indispensible tool for analyzing macromolecules, such as peptides, proteins, nucleic acids and polymers. However, the application of MALDI for the analysis of small molecules (<700 Da) has become the great challenge because of the interference from the conventional matrix in low mass region. To overcome this drawback, more attention has been paid to explore interference-free methods in the past decade. The technique of applying nanomaterials as matrix of laser desorption/ionization (LDI), also called nanomaterial-assisted laser desorption/ionization (nanomaterial-assisted LDI), has attracted considerable attention in the analysis of low-molecular weight compounds in TOF MS. This review mainly summarized the applications of different types of nanomaterials including carbon-based, metal-based and metal-organic frameworks as assisted matrices for LDI in the analysis of small biological molecules, environmental pollutants and other low-molecular weight compounds. Full article

There are numerous studies showing that there is a constant increase in the ocean ambient noise level and the ever-growing demand for developing algorithms for detecting weak signals in ambient noise. In this study, we utilize dynamical and statistical complexity to detect the presence of weak ship noise embedded in ambient noise. The ambient noise and ship noise were recorded in the South China Sea. The multiscale entropy (MSE) method and the complexity-entropy causality plane (C-H plane) were used to quantify the dynamical and statistical complexity of the measured time series, respectively. We generated signals with varying signal-to-noise ratio (SNR) by varying the amplification of a ship signal. The simulation results indicate that the complexity is sensitive to change in the information in the ambient noise and the change in SNR, a finding that enables the detection of weak ship signals in strong background ambient noise. The simulation results also illustrate that complexity is better than the traditional spectrogram method, particularly effective for detecting low SNR signals in ambient noise. In addition, complexity-based MSE and C-H plane methods are simple, robust and do not assume any underlying dynamics in time series. Hence, complexity should be used in practical situations. Full article

Phenylboronic acids can form tight covalent bonds with diol-containing biomolecules. In this work, water-dispersed bifunctional gold nanoparticles (AuNPs) modified with ferrecene (Fc)-derivatized peptides and 4-mercaptophenylboronic acids (MBA) (denoted as Fc–MBA–AuNPs) were synthesized and characterized by UV/vis spectrometry and transmission electron microscopy. To demonstrate the application and the analytical merits of the nanoparticles in biosensing, glycoprotein avidin was tested as a model analyte. Specifically, avidin was captured by the biotin-covered gold electrode via the strong biotin-avidin interaction. Then, Fc–MBA–AuNPs were attached by the captured avidin through the formation of tight covalent bonds between the boronic acid moieties of Fc–MBA–AuNPs and the oligosaccharides of avidin. As a result, a detection limit of 0.2 pM was achieved. We believe that the bifunctional nanoparticles would found many applications in amplified detection of diol-containing species by rational design of the surface chemistry of electrode. Full article