To solve the problem of air hole coating and analyte filling in microstructured optical fiber-based surface plasmon resonance (SPR) sensors, we designed an exposed-core grapefruit fiber (EC-GFs)-based SPR sensor. The exposed section of the EC-GF is coated with a SPR, supporting thin silver film, which can sense the analyte in the external environment. The asymmetrically coated fiber can support two separate resonance peaks (x- and y-polarized peaks) with orthogonal polarizations and x-polarized peak, providing a much higher peak loss than y-polarized, also the x-polarized peak has higher wavelength and amplitude sensitivities. A large analyte refractive index (RI) range from 1.33 to 1.42 is calculated to investigate the sensing performance of the sensor, and an extremely high wavelength sensitivity of 13,500 nm/refractive index unit (RIU) is obtained. The silver layer thickness, which may affect the sensing performance, is also discussed. This work can provide a reference for developing a high sensitivity, real-time, fast-response, and distributed SPR RI sensor. Full article

We propose a temperature sensor design based on surface plasmon resonances (SPRs) supported by filling the holes of a six-hole photonic crystal fiber (PCF) with a silver nanowire. A liquid mixture (ethanol and chloroform) with a large thermo-optic coefficient is filled into the PCF holes as sensing medium. The filled silver nanowires can support resonance peaks and the peak will shift when temperature variations induce changes in the refractive indices of the mixture. By measuring the peak shift, the temperature change can be detected. The resonance peak is extremely sensitive to temperature because the refractive index of the filled mixture is close to that of the PCF material. Our numerical results indicate that a temperature sensitivity as high as 4 nm/K can be achieved and that the most sensitive range of the sensor can be tuned by changing the volume ratios of ethanol and chloroform. Moreover, the maximal sensitivity is relatively stable with random filled nanowires, which will be very convenient for the sensor fabrication. Full article

The miR-221/222 cluster has been demonstrated to function as oncomiR in human cancers. miR-221/222 promotes epithelial-to-mesenchymal transition (EMT) and confers tamoxifen resistance in breast cancer. However, the effects and mechanisms by which miR-221/222 regulates breast cancer aggressiveness remain unclear. Here we detected a much higher expression of miR-221/222 in highly invasive basal-like breast cancer (BLBC) cells than that in non-invasive luminal cells. A microRNA dataset from breast cancer patients indicated an elevated expression of miR-221/222 in BLBC subtype. S-phase entry of the cell cycle was associated with the induction of miR-221/222 expression. miRNA inhibitors specially targeting miR-221 or miR-222 both significantly suppressed cellular migration, invasion and G₁/S transition of the cell cycle in BLBC cell types. Proteomic analysis demonstrated the down-regulation of two tumor suppressor genes, suppressor of cytokine signaling 1 (SOCS1) and cyclin-dependent kinase inhibit 1B (CDKN1B), by miR-221/222. This is the first report to reveal miR-221/222 regulation of G₁/S transition of the cell cycle. These findings demonstrate that miR-221/222 contribute to the aggressiveness in control of BLBC. Full article

In this paper, a reflective photonic crystal fiber (PCF) sensor probe for temperature measurement has been demonstrated both theoretically and experimentally. The performance of the device depends on the intensity modulation of the optical signal by liquid mixtures infiltrated into the air holes of commercial LMA-8 PCFs. The effective mode field area and the confinement loss of the probe are both proved highly temperature-dependent based on the finite element method (FEM). The experimental results show that the reflected power exhibits a linear response with a temperature sensitivity of about 1 dB/°C. The sensor probe presents a tunable temperature sensitive range due to the concentration of the mixture components. Further research illustrates that with appropriate mixtures of liquids, the probe could be developed as a cryogenic temperature sensor. The temperature sensitivity is about 0.75 dB/°C. Such a configuration is promising for a portable, low-power and all-in-fiber device for temperature or refractive index monitoring in chemical or biosensing applications. Full article

A large-mode-area polymer photonic crystal fiber made of polymethyl methacrylate with the cladding having only one layer of air holes near the edge of the fiber is designed and proposed to be used in surface plasmon resonance sensors. In such sensor, a nanoscale metal film and analyte can be deposited on the outer side of the fiber instead of coating or filling in the holes of the conventional PCF, which make the real time detection with high sensitivity easily to realize. Moreover, it is relatively stable to changes of the amount and the diameter of air holes, which is very beneficial for sensor fabrication and sensing applications. Numerical simulation results show that under the conditions of the similar spectral and intensity sensitivity of 8.3 × 10⁻⁵–9.4 × 10⁻⁵ RIU, the confinement loss can be increased dramatically. Full article

A kind of surface plasmon resonance sensor based on grapefruit photonic crystal fiber (PCF) filled with different numbers of silver nanowires has been studied in this paper. The surface plasmon resonance modes and the sensing properties are investigated comprehensively using the finite element method (FEM). The simulation results show that the intensity sensitivity is related to nanowire numbers and the distance between two nanowires. The optimum value obtained is 2,400 nm/RIU, corresponding to a resolution of 4.51 × 10⁻⁵ RIU with a maximum distance of 2 μm. To a certain extent, the PCF filled with more nanowires is better than with just one. Furthermore, the air holes of grapefruit PCF are large enough to operate in practice. Moreover, the irregularity of the filled nanowires has no effect on sensitivity, which will be very convenient for the implementation of experiments. Full article