The influence of optical parameters on the performance of closed-loop optical voltage sensors (OVSs) based on Pockels effect is analyzed and a control algorithm is proposed to suppress the nonlinearity caused by the unideal parameters of optical devices for optimizing the detection precision of OVSs. First, a quantified model of the feedback phase demonstrates how the optical parameters of optical devices (including light source, polarizer, 45° fusion point, Faraday rotator and half-wave plate) result in the nonlinearity of closed-loop OVSs. Then, the parameter indexes of different optical devices are put forward to instruct the manufacturing process of the optical system. Furthermore, a closed-loop control algorithm is investigated to improve the measurement accuracy of nonlinear OVSs considering the unideal parameters. The experiment results indicate that additional bias caused by undesirable optical parameters is obviously decreased so that the measurement accuracy of OVSs satisfies the demand of IEC60044-3 for 0.1 level measurement accuracy, which verifies the effectiveness and correctness of the methods for suppressing the impact of unideal optical parameters on OVSs. Full article

Understanding household livelihood strategies is pivotal to minimize rural poverty in the least developed countries like Nepal. This study is an attempt to assess livelihood strategies pursued by rural households, investigate the most remunerative strategy, and identify the factors that influence a household’s choice of better strategies in rural Nepal. Primary data collected in 453 households from three villages of central Nepal are analyzed quantitatively within a sustainable livelihood framework. This study categorized households into five main livelihood strategy groups. The results showed that the majority (61%) of the households diversified their income to non-farm sources. Livelihood diversification to business/enterprise strategies adopted by 16% of the households is the most remunerative strategy followed by commercial farming that includes 13% of the sample and are more relevant to poverty reduction. Land holding, education, agriculture and skill training, access to credit, and proximity to the road and market center are the major influencing factors on the adoption of higher returning livelihood strategies. Stimulating poor households to follow market-oriented farm and non-farm activities by improving access to education, vocational training, rural credit, and rural infrastructures is momentous for reducing poverty in the rural areas of central Nepal. Full article

The developmentally regulated brain protein drebrin (Dbn) is a functional protein involved with long-term memory formation and is widely distributed in brain neurons, especially in the dendritic spines. A noticeable decline of this protein has been found in the hippocampus and cortex of patients with Alzheimer’s disease (AD), yet the relationship between Dbn and AD has not been fully understood. In the present study, we examined how down-regulation of Dbn impacts the progression of AD in experimental animals. Accordingly, we injected Dbn interference vector (rAAV-mDbn1 ShRNA) into the hippocampus of three-month old APP(swe)/PS1(ΔE9) mice (APP/PS1 mice) and then successfully down-regulated Dbn expression in this brain region. Behavioral tests, including the Morris water maze test, the open field test, and the novel object test were conducted when the animals were nine months old. Subsequently, MicroPET/CT imaging to monitor glucose metabolism was done. We then investigated Aβ, GFAP, PSD-95, MAP2, vimentin, Cox43, and Syn1 expressions in the brain of the experimental animals via immunohistochemical or immunofluorescence methods. We found that AD mice with a low expression of Dbn performed poorly in the behavioral tests and showed decreased glucose utilization. In the brains of these animals, we detected a slight increase of Aβ, GFAP and vimentin and a significant decline of PSD-95. Altogether our data warrant further studies to elucidate the effect of Dbn on the development and progression of AD. Full article

The flotation and adsorption of a new polysaccharide konjac gum (KG) on pyrite and talc in the presence of pre-adsorbed potassium butyl xanthate (PBX) is investigated. The micro-flotation results show that KG is a quality depressant for talc and that conditioning the minerals initially with PBX before KG will increase the recovery difference between pyrite and talc. The results of artificially mixing the minerals show that compared with adding KG before PBX, when minerals are pre-adsorbed with PBX, the grade and the recovery of sulfur (S) increases by 1.96% and 5.44%, respectively. The contact angle results show that the addition of PBX before KG will increase the contact angles of pyrite, but the addition order of KG/PBX has little influence on the contact angles of talc. The adsorption tests show that KG can adsorb on pyrite and talc surfaces, while PBX can only adsorb on the pyrite surface. The addition order of KG/PBX affects the adsorption of KG and PBX on the pyrite surface but not on the talc surface. Fourier transform infrared (FTIR) spectra analysis further demonstrates the chemical adsorption of KG on pyrite and talc surfaces, while PBX chemisorbs on the pyrite surface. Based on these analyses, a schematic illustration of the reagent adsorption forms on pyrite and talc surfaces is drawn to explain the competitive adsorption of KG and PBX on mineral surfaces. Full article

The nickel(II) catalyst has manifested higher catalytic activity compared to that of other late transition metal catalysts for norbornene polymerization. Therefore, several structurally similar trans-nickel(II) compounds of N,O-chelate bidentate ligands were synthesized and characterized. Both the electronic effect and the steric hindrance influence polymerization. The molecular structures of 2, 4 and 5 were further confirmed by single-crystal X-ray diffraction. Full article

An improved temperature-insensitive optical voltage sensor (OVS) with a reciprocal dual-crystal sensing method is proposed. The inducing principle of OVS reciprocity degradation is expounded by taking the different temperature fields of two crystals and the axis-errors of optical components into consideration. The key parameters pertaining to the system reciprocity degeneration in the dual-crystal sensing unit are investigated in order to optimize the optical sensing model based on the Maxwell's electromagnetic theory. The influencing principle of axis-angle errors on the system nonlinearity in the Pockels phase transfer unit is analyzed. Moreover, a novel axis-angle compensation method is proposed to improve the OVS measurement precision according to the simulation results. The experiment results show that the measurement precision of OVS is superior to ±0.2% in the temperature range from −40 °C to +60 °C, which demonstrates the excellent temperature stability of the designed voltage sensing system. Full article

An interprovincial boundary region is a new subject of economic disparity study in China. This study explored the multi-scale spatio-temporal dynamics of economic development from 1995 to 2010 in the interprovincial boundary region of Sichuan-Yunnan-Guizhou, a mountain area and also the junction area of Tibetan Plateau, Hengduan Mountain, Yungui Plateau and Sichuan Basin in southwestern China. A quantitative study on county GDP per capita for different scales of administrative regions was conducted using the Theil index, Markov chains, a geographic information system and exploratory spatial data analysis. Results indicated that the economic disparity was closely related with geographical unit scale in the study area: the smaller the unit, the bigger the disparity, and the regional inequality gradually weakened over time. Moreover, significant positive spatial autocorrelation and clustering of economic development were also found. The spatial pattern of economic development presented approximate circle structure with two cores in the southwest and northeast. The Panxi region in the southwest core and a part of Hilly Sichuan Basin in the northeast core were considered to be hot spots of economic development. Most areas in the east and central region were persistently trapped in the low level of a balanced development state, with a poverty trap being formed in the central and south part. Geographical conditions and location, administrative barriers and the lack of effective growth poles may be the main reasons for the entire low level of balanced development. Our findings suggest that in order to achieve a high level of balanced development, attention should be paid beyond developing transportation and other infrastructure. Breaking down the rigid shackles of administrative districts that hinder trans-provincial cooperation and promoting new regional poles in the Yunnan-Guizhou region may have great significance for the study area. Full article

Male and female patients with schizophrenia show significant differences in a number of important clinical features, yet the neural substrates of these differences are still poorly understood. Here we explored the sex differences in the brain functional aberrations in 124 treatment-naïve patients with first-episode schizophrenia (61 males), compared with 102 age-matched healthy controls (50 males). Maps of degree centrality (DC) and amplitude of low-frequency fluctuations (ALFF) were constructed using resting-state functional magnetic resonance imaging data and compared between groups. We found that: (1) Selective DC reduction was observed in the right putamen (Put_R) in male patients and the left middle frontal gyrus (MFG) in female patients; (2) Functional connectivity analysis (using Put_R and MFG as seeds) found that male and female patients have disturbed functional integration in two separate networks, i.e., the sensorimotor network and the default mode network; (3) Significant ALFF alterations were also observed in these two networks in both genders; (4) Sex specific brain functional alterations were associated with various symptoms in patients. These results suggested that sex-specific patterns of functional aberration existed in schizophrenia, and these patterns were associated with the clinical features both in male and female patients. Full article

Cell phones and smart phones can be reconfigured as biomedical sensor devices but this requires specialized add-ons. In this paper we present a simple cell phone-based portable bioassay platform, which can be used with fluorescent assays in solution. The system consists of a tablet, a polarizer, a smart phone (camera) and a box that provides dark readout conditions. The assay in a well plate is placed on the tablet screen acting as an excitation source. A polarizer on top of the well plate separates excitation light from assay fluorescence emission enabling assay readout with a smartphone camera. The assay result is obtained by analysing the intensity of image pixels in an appropriate colour channel. With this device we carried out two assays, for collagenase and trypsin using fluorescein as the detected fluorophore. The results of collagenase assay with the lowest measured concentration of 3.75 µg/mL and 0.938 µg in total in the sample were comparable to those obtained by a microplate reader. The lowest measured amount of trypsin was 930 pg, which is comparable to the low detection limit of 400 pg for this assay obtained in a microplate reader. The device is sensitive enough to be used in point-of-care medical diagnostics of clinically relevant conditions, including arthritis, cystic fibrosis and acute pancreatitis. Full article

Automatically identifying the new equipment after its integration and adjusting operation strategy to realize “plug and play” functionality are becoming essential for micro-grid operations. In order to improve and perfect the micro-grid “plug and play” function with the increased amount of equipment with different information protocols and more diverse system applications, this paper presents a solution for adaptive micro-grid operation based on IEC 61850, and proposes the design and specific implementation methods of micro-grid “plug and play” function and system operation mode conversion in detail, by using the established IEC 61850 information model of a micro-grid. Actual operation tests based on the developed IED and micro-grid test platform are performed to verify the feasibility and validity of the proposed solution. The tests results show that the solution can automatically identify the IEC 61850 information model of equipment after its integration, intelligently adjust the operation strategies to adapt to new system states and achieves a reliable system operation mode conversion. Full article

Evapotranspiration (ET) plays a key role in water resource management. It is important to understand the ET spatio-temporal pattern of South Asia for understanding and anticipating serious water resource shortages. In this study, daily ET in 2008 was estimated over South Asia by using MODerate Resolution Imaging Spectroradiometer (MODIS) products combined with field observations and Global Land Data Assimilation System (GLDAS) product through Surface Energy Balance System (SEBS) model. Monthly ET data were calculated based on daily ET and evaluated by the GLDAS ET data. Good agreements were found between two datasets for winter months (October to February) with R² from 0.5 to 0.7. Spatio-temporal analysis of ET was conducted. Ten specific sites with different land cover types at typical climate regions were selected to analyze the ET temporal change pattern, and the result indicated that the semi-arid or arid areas in the northwest had the lowest average daily ET (around 0.3 mm) with a big fluctuation in the monsoon season, while the sites in the Indo-Gangetic Plain and in southern India has bigger daily ET (more than 3 mm) due to a large water supplement. It is suggested that the monsoon climate has a large impact on ET spatio-temporal variation in the whole region. Full article

Working memory deficit is the core neurocognitive disorder in schizophrenia patients. To identify the factors underlying working memory deficit in schizophrenia patients and to explore the implication of possible genes in the working memory using genome-wide association study (GWAS) of schizophrenia, computerized delay-matching-to-sample (DMS) and whole genome genotyping data were obtained from 100 first-episode, treatment-naïve patients with schizophrenia and 140 healthy controls from the Mental Health Centre of the West China Hospital, Sichuan University. A composite score, delay-matching-to-sample total correct numbers (DMS-TC), was found to be significantly different between the patients and control. On associating quantitative DMS-TC with interactive variables of groups × genotype, one SNP (rs1411832), located downstream of YWHAZP5 in chromosome 10, was found to be associated with the working memory deficit in schizophrenia patients with lowest p-value (p = 2.02 × 10⁻⁷). ConsensusPathDB identified that genes with SNPs for which p values below the threshold of 5 × 10⁻⁵ were significantly enriched in GO:0007155 (cell adhesion, p < 0.001). This study indicates that working memory, as an endophenotype of schizophrenia, could improve the efficacy of GWAS in schizophrenia. However, further study is required to replicate the results from our study. Full article

Diabetes mellitus (DM) has been recognized as a major health problem. Emodin (Emo) has been reported to exhibit protective effects against diabetic nephropathy. However, little has been known about the effect of Emo on diabetic cardiomyopathy (DCM). A type 2 DM model was induced in rats by low dose streptozotocin (STZ) combined with high energy intake. We found that Emo-treated groups displayed significantly higher body weight (BW) and lower heart weight (HW)/BW. Furthermore, Emo could significantly decrease blood glucose, total cholesterol (TG) levels, and triglyceride (TC) levels in diabetic rats. Moreover, the Emo-treated group showed a marked increase in heart rate (HR) and showed lower left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular posterior wall thickness (LWPWT), and interventricular septal diastolic wall thickness (IVSD). Emo induced a significant increase in phosphorylation of Akt and GSK-3β in myocardium. These results suggest that Emo may have great therapeutic potential in the treatment of DCM by Akt/GSK-3β signaling pathway. Full article

Our previous studies show that adenosine-induced apoptosis is involved in endoplasmic reticulum stress in HepG2 cells. In this study, we have investigated whether knockdown of GRP78 by short hairpin RNA (shRNA) increases the cytotoxic effects of adenosine in HepG2 cells. The adenovirus vector-delivered shRNA targeting GRP78 (Ad-shGRP78) was constructed and transfected into HepG2 cells. RT-PCR assay was used to determine RNA interference efficiency. Effects of knockdown of GRP78 on adenosine-induced cell viabilities, cell-cycle distribution and apoptosis, as well as relative protein expressions were determined by flow cytometry and/or Western blot analysis. The intracellular Ca²⁺ concentration was detected by laser scanning confocal microscope. Mitochondrial membrane potential (ΔΨm) was measured by a fluorospectrophotometer. The results revealed that GRP78 mRNA was significantly downregulated by Ad-shGRP78 transfection. Knockdown of GRP78 enhanced HepG2 cell sensitivity to adenosine by modulating G0/G1 arrest and stimulating Bax, Bak, m-calpain, caspase-4 and CHOP protein levels. Knockdown of GRP78 worsened cytosolic Ca²⁺ overload and ΔΨm loss. Knockdown of caspase-4 by shRNA decreased caspase-3 mRNA expression and cell apoptosis. These findings indicate that GRP 78 plays a protective role in ER stress-induced apoptosis and show that the combination of chemotherapy drug and RNA interference adenoviruses provides a new treatment strategy against malignant tumors. Full article

The pattern of vegetation change in response to global change still remains a controversial issue. A Normalized Difference Vegetation Index (NDVI) dataset compiled by the Global Inventory Modeling and Mapping Studies (GIMMS) was used for analysis. For the period 1982–2006, GIMMS-NDVI analysis indicated that monthly NDVI changes show homogenous trends in middle and high latitude areas in the northern hemisphere and within, or near, the Tropic of Cancer and Capricorn; with obvious spatio-temporal heterogeneity on a global scale over the past two decades. The former areas featured increasing vegetation activity during growth seasons, and the latter areas experienced an even greater amplitude in places where precipitation is adequate. The discussion suggests that one should be cautious of using the NDVI time-series to analyze local vegetation dynamics because of its coarse resolution and uncertainties. Full article