In response to the call of the Chinese government to support low-carbon development, the issue has come to the view gradually as to whether the behaviors of banks’ green credit will contribute to easing their own credit risk. To reflect the behaviors of green credit of banks in detail, an indicator, named the carbon intensity of loans (CIL), is first proposed in this paper to measure the carbon emissions with association of the loans for commercial banks, on basis of the series of the input–output table. Then, a panel data model is used to explore the relationship between CIL and non-performing loan ratio, which measures the credit risk of banks. Based on the data of China’s commercial banks from 2007 to 2014, an empirical study has been conducted to investigate the impacts of CIL upon the non-performing loan ratio from a microscopic-level perspective. The result indicates that CIL has a positive effect on the non-performing loan ratio of banks. Since CIL is considered a significant indicator for the banks’ green credit, this paper comes to a conclusion that the green credit policy not only contributes to achieving of the emission-reduction targets for the society, but also promotes the development of banks’ credit risk. Full article

Many species of endoparasitoid wasps provide biological control services in agroecosystems. Although there is a great deal of information on the ecology and physiology of host/parasitoid interactions, relatively little is known about the protein composition of venom and how specific venom proteins influence physiological systems within host insects. This is a crucial gap in our knowledge because venom proteins act in modulating host physiology in ways that favor parasitoid development. Here, we identified 37 possible venom proteins from the polydnavirus-carrying endoparasitoid Cotesia chilonis by combining transcriptomic and proteomic analyses. The most abundant proteins were hydrolases, such as proteases, peptidases, esterases, glycosyl hydrolase, and endonucleases. Some components are classical parasitoid venom proteins with known functions, including extracellular superoxide dismutase 3, serine protease inhibitor and calreticulin. The venom contains novel proteins, not recorded from any other parasitoid species, including tolloid-like proteins, chitooligosaccharidolytic β-N-acetylglucosaminidase, FK506-binding protein 14, corticotropin-releasing factor-binding protein and vascular endothelial growth factor receptor 2. These new data generate hypotheses and provide a platform for functional analysis of venom components. Full article

Reducing carbon emissions is a major concern for China’s future. This paper explores the embodied carbon footprint of Chinese final demand from the point of view of industries. It uses the Matrix Transformation Technique (MTT) to update the input-output table series from 1992 to 2020 in China. Then, we measure the embodied carbon emissions for the period 1992–2020 from 29 industry producers to the final demand, covering urban and rural residential consumption, government consumption, fixed capital formation, and net exports. The results show that construction, other services, wholesale, retail trade, accommodation and catering, industrial machinery and equipment, transport, storage and postal services, and manufacture of foods and tobacco are the industries with the greatest carbon emissions from producers, while fixed capital formation and urban consumption are the largest emitters from the perspective of final demand. The embodied carbon emission multipliers for most of the industries are decreasing, while the total carbon emissions are increasing each year. The ratio of emissions from residential consumption in terms of total emissions is decreasing. Each industry has a different main final demand-driven influencing factor on emission and, for each type of final demand, there are different industries with higher emissions. Full article

In this work we directly synthesized molybdenum disulfide (MoS₂) nanosheets on carbon nanotube film (MoS₂@CNT) via a two-step chemical vapor deposition method (CVD). By etching the obtained MoS₂@CNT into 10% wt HNO₃, the morphology of MoS₂ decorated on CNT bundles was modulated, resulting in more catalytic active MoS₂ edges being exposed for significantly enhanced electrochemical performance. Our results revealed that an 8 h acid etching sample exhibited the best performance for the oxygen evolution reaction, i.e., the current density reached 10 mA/cm² under 375 mV over-potential, and the tafel slope was as low as 94 mV/dec. The enhanced behavior was mainly originated from the more catalytic sites in MoS₂ induced by the acid etching treatment and the higher conductivity from the supporting CNT films. Our study provides a new route to produce two-dimensional layers on CNT films with tunable morphology, and thus may open a window for exploring its promising applications in the fields of catalytic-, electronic-, and electrochemical-related fields. Full article

Insect host/parasitoid interactions are co-evolved systems in which host defenses are balanced by parasitoid mechanisms to disable or hide from host immune effectors. Here, we report that Pteromalus puparum venom impairs the antimicrobial activity of its host Pieris rapae. Inhibition zone results showed that bead injection induced the antimicrobial activity of the host hemolymph but that venom inhibited it. The cDNAs encoding cecropin and lysozyme were screened. Relative quantitative PCR results indicated that all of the microorganisms and bead injections up-regulated the transcript levels of the two genes but that venom down-regulated them. At 8 h post bead challenge, there was a peak in the transcript level of the cecropin gene, whereas the peak of lysozyme gene occurred at 24 h. The transcripts levels of the two genes were higher in the granulocytes and fat body than in other tissues. RNA interference decreased the transcript levels of the two genes and the antimicrobial activity of the pupal hemolymph. Venom injections similarly silenced the expression of the two genes during the first 8 h post-treatment in time- and dose-dependent manners, after which the silence effects abated. Additionally, recombinant cecropin and lysozyme had no significant effect on the emergence rate of pupae that were parasitized by P. puparum females. These findings suggest one mechanism of impairing host antimicrobial activity by parasitoid venom. Full article

Chitin-binding proteins (CBPs) are present in many species and they act in a variety of biological processes. We analyzed a Pteromalus puparum venom apparatus proteome and transcriptome and identified a partial gene encoding a possible CBP. Here, we report cloning a full-length cDNA of a sequence encoding a chitin-binding-like protein (PpCBP) from P. puparum, a pupal endoparasitoid of Pieris rapae. The cDNA encoded a 96-amino-acid protein, including a secretory signal peptide and a chitin-binding peritrophin-A domain. Phylogenetic analysis of chitin binding domains (CBDs) of cuticle proteins and peritrophic matrix proteins in selected insects revealed that the CBD of PpCBP clustered with the CBD of Nasonia vitripennis. The PpCBP is specifically expressed in the venom apparatus of P. puparum, mostly in the venom gland. PpCBP expression was highest at day one after adult eclosion and much lower for the following five days. We produced a recombinant PpCBP and binding assays showed the recombinant protein selectively binds chitin but not cellulose in vitro. We infer that PpCBP serves a structural role in the venom reservoir, or may be injected into the host to help wound healing of the host exoskeleton. Full article

This research was supported by grants from the National Program on Key Basic Research Projects (973 Program, 2013CB127600), the National Natural Science Foundation of China (Grant Nos. 31272098, 31472038, 31402018), the Research Fund for the Doctoral Program of Higher Education of China (Grant Number 2012010113004), the National Science Fund for Innovative Research Groups of Biological Control (Grant No. 31321063), the Zhejiang Provincial Natural Science Foundation of China (Grant Number Y14C140006) and the Fundamental Research Funds for the Central Universities (Grant Number 2014FZA6014). [...] Full article

Two cDNA sequences of Kazal-type serine protease inhibitors (KSPIs) in Nasonia vitripennis, NvKSPI-1 and NvKSPI-2, were characterized and their open reading frames (ORFs) were 198 and 264 bp, respectively. Both NvKSPI-1 and NvKSPI-2 contained a typical Kazal-type domain. Real-time quantitative PCR (RT-qPCR) results revealed that NvKSPI-1 and NvKSPI-2 mRNAs were mostly detected specifically in the venom apparatus, while they were expressed at lower levels in the ovary and much lower levels in other tissues tested. In the venom apparatus, both NvKSPI-1 and NvKSPI-2 transcripts were highly expressed on the fourth day post eclosion and then declined gradually. The NvKSPI-1 and NvKSPI-2 genes were recombinantly expressed utilizing a pGEX-4T-2 vector, and the recombinant products fused with glutathione S-transferase were purified. Inhibition of recombinant GST-NvKSPI-1 and GST-NvKSPI-2 to three serine protease inhibitors (trypsin, chymotrypsin, and proteinase K) were tested and results showed that only NvKSPI-1 could inhibit the activity of trypsin. Meanwhile, we evaluated the influence of the recombinant GST-NvKSPI-1 and GST-NvKSPI-2 on the phenoloxidase (PO) activity and prophenoloxidase (PPO) activation of hemolymph from a host pupa, Musca domestica. Results showed PPO activation in host hemolymph was inhibited by both recombinant proteins; however, there was no significant inhibition on the PO activity. Our results suggested that NvKSPI-1 and NvKSPI-2 could inhibit PPO activation in host hemolymph and trypsin activity in vitro. Full article