Burns can cause tremendous economic problems associated with irreparable harm to patients and their families. To characterize marine collagen peptides (MCPs) from the skin of Nile tilapia (Oreochromis niloticus), molecular weight distribution and amino acid composition of MCPs were determined, and Fourier transform infrared spectroscopy (FTIR) was used to analyze the chemical structure. Meanwhile, to evaluate the wound healing activity, in vitro and in vivo experiments were carried out. The results showed that MCPs prepared from the skin of Nile tilapia by composite enzymatic hydrolysis were composed of polypeptides with different molecular weights and the contents of polypeptides with molecular weights of less than 5 kDa accounted for 99.14%. From the amino acid composition, the majority of residues, accounting for over 58% of the total residues in MCPs, were hydrophilic. FTIR indicated that the main molecular conformations inside MCPs were random coil. In vitro scratch assay showed that there were significant effects on the scratch closure by the treatment of MCPs with the concentration of 50.0 μg/mL. In the experiments of deep partial-thickness scald wound in rabbits, MCPs could enhance the process of wound healing. Therefore, MCPs from the skin of Nile tilapia (O. niloticus) have promising applications in wound care. Full article

Background: A previous study suggested that activin A inhibited myocardial cell apoptosis. This study thus aimed to explore the effects of the activin A–follistatin system on myocardial cell apoptosis in heart failure (HF) rats in order to determine whether or not the mechanism operates through the endoplasmic reticulum stress (ERS) pathway. Methods: Myocardial infarction (MI) by vascular deprivation was used to induce HF. The enzyme-linked immunosorbent assay was used to detect activin A, follistatin and brain natriuretic peptide (BNP) contents in serum. Immunohistochemical staining for activin A, follistatin, CCAAT-enhancer-binding protein (C/EBP) homologous protein (CHOP) and caspase-3 was performed on the myocardial tissue. The activin A-stimulated apoptosis of H9c2 cells was tested by flow cytometry. Western blot was used to detect the expression levels of activin A, follistatin and ERS-related proteins. Results: It was found that the high expression of activin A could cause activin A–follistatin system imbalance, inducing myocardial cell apoptosis via ERS in vivo. When HF developed to a certain stage, the expression of follistatin was upregulated to antagonize the expression of activin A. Activin A inhibited cardiomyocyte apoptosis with a low concentration and promoted apoptosis with a high concentration in vitro, also via ERS. Conclusion: Activin A–follistatin system participated in ERS-mediated myocardial cell apoptosis in HF. Full article

Lung cancer is the deadliest cancer in the United States, killing roughly one of four cancer patients in 2016. While it is well-established that lung cancer is caused primarily by environmental effects (particularly tobacco smoking), there is evidence for genetic susceptibility. Lung cancer has been shown to aggregate in families, and segregation analyses have hypothesized a major susceptibility locus for the disease. Genetic association studies have provided strong evidence for common risk variants of small-to-moderate effect. Rare and highly penetrant alleles have been identified by linkage studies, including on 6q23–25. Though not common, some germline mutations have also been identified via sequencing studies. Ongoing genomics studies aim to identify additional high penetrance germline susceptibility alleles for this deadly disease. Full article

Taxifolin is a ubiquitous bioactive constituent of foods and herbs. To thoroughly explore its metabolism in vivo, an HPLC-ESI-IT-TOF-MSⁿ method combined with specific metabolite detection strategy was used to detect and identify the metabolites of taxifolin in rats. Of the 191 metabolites tentatively identified, 154 were new metabolites, 69 were new compounds and 32 were dimers. This is the first report of the in vivo biotransformation of a single compound into more than 100 metabolites. Furthermore, acetylamination and pyroglutamic acid conjugation were identified as new metabolic reactions. Seventeen metabolites were found to have various taxifolin-related bioactivities. The potential targets of taxifolin and 63 metabolites were predicted using PharmMapper, with results showing that more than 60 metabolites have the same five targets. Metabolites with the same fragment pattern may have the same pharmacophore. Thus these metabolites may exert the same pharmacological effects as taxifolin through an additive effect on the same drug targets. This observation indicates that taxifolin is bioactive not only in the parent form, but also through its metabolites. These findings enhance understanding of the metabolism and effective forms of taxifolin and may provide further insight of the beneficial effects of taxifolin and its derivatives. Full article

A noble-metal free photocatalyst consisting of WS₂ and TiSi₂ being used for hydrogen evolution under visible light irradiation, has been successfully prepared by in-situ formation of WS₂ on the surface of TiSi₂ in a thermal reaction. The obtained samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The results demonstrate that WS₂ moiety has been successfully deposited on the surface of TiSi₂ and some kind of chemical bonds, such as Ti-S-W and Si-S-W, might have formed on the interface of the TiSi₂ and WS₂ components. Optical and photoelectrochemical investigations reveal that WS₂/TiSi₂ composite possesses lower hydrogen evolution potential and enhanced photogenerated charge separation and transfer efficiency. Under 6 h of visible light (λ > 420 nm) irradiation, the total amount of hydrogen evolved from the optimal WS₂/TiSi₂ catalyst is 596.4 μmol·g⁻¹, which is around 1.5 times higher than that of pure TiSi₂ under the same reaction conditions. This study shows a paradigm of developing the effective, scalable and inexpensive system for photocatalytic hydrogen generation. Full article

With the rapid development of microgrids (MGs) in recent years, it is anticipated that combinations of multiple microgrids—multi-microgrids (MMGs)—will gradually become a new form of power grid. A safe and efficient black start strategy for MMGs is in urgent demand because of their complicated structure and control systems. In this paper, first, we analyze the topology and control system of residential-type MMGs with three-phase/single-phase (TP/SP) architecture. Second, a black start strategy based on a hierarchical control scheme is presented, including the selection strategy for the main power supply and master microgrid, the stand-alone operation strategy, and the grid-connected operation strategy. After the selection of the main power supplies, the master MG is determined. Hereby, all sub-microgrids (SMGs) execute the stand-alone algorithm. When the synchronous connection condition is satisfied, the slave SMGs connect to the master MG who provides the voltage and frequency support. Meanwhile, the control algorithm transfers to the grid-connected algorithm, with the grid dispatching value set to zero. Finally, experimental results from the MMG experimental setup in the Clean Energy Technology Laboratory (CETLAB) are presented to verify the effectiveness and feasibility of the proposed black start strategy. Full article

In the present work, different concentrations of Cu ion (1, 2, 5 and 10 wt %) were doped in the TiO₂ film by a sol-gel method and dip coating process. The morphology of the Cu-doped TiO₂ films were characterized by scanning electron microscopy (SEM) and the results showed that the doped Cu made no change to the TiO₂ films. The nitric oxide (NO) release experiment showed that these Cu-doped surfaces showed the ability of catalytic decomposition of exogenous donor S-nitroso-N-acetyl-penicillamine (SNAP) to generate (NO). Based on fluorescence analysis and CCK-8 quantitative results, such films had the ability to inhibit smooth muscle cells adhesion, proliferation and migration with SNAP in vitro. The macrophage adhesion assay and anti-bacterial test proved that such Cu-doped TiO₂ films also possessed anti-inflammatory and anti-bacterial abilities. All the abilities above showed positive correlation with the amounts of the doped Cu. This study suggested that the Cu-doped TiO₂ films were capable of generating physiological levels of NO in the presence of endogenous donor S-nitrosothiols (RSNO), endowing the TiO₂ films with anti-hyperplasia, anti-inflammatory and anti-bacterial abilities. Full article

This paper presents a technique to generate cirrus optical depth and particle effective size estimates from the cloud emissivities at 8.5, 11 and 12 μm contained in the Collection-6 (C6) MYD06 cloud product. This technique employs the latest scattering models and scattering radiative transfer approximations to estimate cloud optical depth and particle effective size using efficient analytical formulae. Two scattering models are tested. The first is the same scattering model as that used in the C6 MYD06 solar reflectance products. The second model is an empirical model derived from radiometric consistency. Both models are shown to generate optical depths that compare well to those from constrained CALIPSO retrievals and MYD06. In terms of effective radius retrievals, the results from the radiometric empirical model agree more closely with MYD06 than those from the C6 model. This analysis is applied to AQUA/MODIS data collocated with CALIPSO/CALIOP during January 2010. Full article

Lung cancer is the leading cause of cancer-related death in the United States. Here, we evaluated the potential clinical utility of soluble human epidermal growth factor receptor 2 (sHER2) for the risk assessment, screening, and diagnosis of non-small cell lung cancer (NSCLC) using an unmatched case-control study design. Serum sHER2 concentrations were measured by immunoassay in 244 primary NSCLC cases and 218 healthy controls. Wilcoxon rank-sum tests, logistic regression models, and receiver operating characteristic plots were used to assess whether sHER2 is associated with lung cancer. Median serum sHER2 concentrations are higher in patients with adenocarcinoma than squamous cell carcinoma regardless of gender, and sHER2 is a weak, independent biomarker of adenocarcinoma, but not of squamous cell carcinoma, adjusted for age and gender. The age-adjusted relative risk (odds) of adenocarcinoma is 3.95 (95% CI: 1.22, 12.81) and 7.93 (95% CI: 2.26, 27.82) greater for women and men with high sHER2 concentrations (≥6.60 ng/mL) vs. low sHER2 concentrations (≤1.85 ng/mL), respectively. When adjusted for each other, sHER2, age, and gender discern healthy controls from patients with primary adenocarcinomas of the lung with 85.9% accuracy. We conclude that even though serum sHER2 is not a strong, stand-alone discriminatory biomarker of adenocarcinoma, sHER2 may be a useful, independent covariate in multivariate risk assessment, screening, and diagnostic models of lung cancer. Full article

Retrievals of ice cloud properties using infrared measurements at 3.7, 6.7, 7.3, 8.5, 10.8, and 12.0 mm can provide consistent results regardless of solar illumination, but are limited to cloud optical thicknesses t < ~6. This paper investigates the variations in radiances at these wavelengths over a deep convective cloud system for their potential to extend retrievals of t and ice particle size D_e to optically thick clouds. Measurements from an imager, an interferometer, the Cloud Physics Lidar (CPL), and the Cloud Radar System (CRS) aboard the NASA ER-2 aircraft during the NASA TC⁴ (Tropical Composition, Cloud and Climate Coupling) experiment flight during 5 August 2007, are used to examine the retrieval potential of infrared radiances over optically thick ice clouds. Simulations based on coincident in situ measurements and combined cloud t from CRS and CPL measurements are comparable to the observations. They reveal that brightness temperatures at these bands and their differences (BTD) are sensitive to t up to ~20 and that for ice clouds having t > 20, the 3.7–10.8 µm and 3.7–6.7 µm BTDs are the most sensitive to D_e. Satellite imagery appears to be consistent with these results suggesting that t and D_e could be retrieved for greater optical thicknesses than previously assumed. But, because of sensitivity of the BTDs to uncertainties in the atmospheric profiles of temperature, humidity, and ice water content, and sensor noise, exploiting the small BTD signals in retrieval algorithms will be very challenging. Full article