Scattering mechanism (SM) analysis is a promising technique for ship detection and classification in polarimetric SAR (PolSAR) images. In this paper, a four-component model-based decomposition method incorporating surface, double-bounce, volume and cross-polarized components is proposed for analyzing the SMs of ships. A novel cross-polarized scattering component capable of discriminating between the HV scattering power generated by the oriented scatterers on ships from that by volume scatterers is proposed as a means to address the problem of volume scattering power overestimation. In the decomposition stage, by taking into account both the real and imaginary parts of the elements ${\langle \left[T\right]\rangle }^{HV}(1,3)$ and ${\langle \left[T\right]\rangle }^{HV}(2,3)$ of the observed coherency matrix, the proposed cross-polarized component can preserve the reflection asymmetry information completely, which is an essential property of man-made targets, such as ships. Based on the proposed decomposition method and an analysis of the different SMs between ships and sea clutter, a novel ship detection metric defined as $\mathbb{M}=ln\frac{P_d+P_c}{P_s}$ is proposed. Experimental results conducted on RadarSat-2 quad-polarimetric data validate the proposed four-component decomposition method as being more suitable for analyzing the SMs of ship targets than the existing helix matrix-based decomposition methods. Additionally, we find that the proposed ship detection metric can effectively enhance the signal-to-clutter ratio (SCR) and improve ship detection performance. Full article

Based on land use and land cover (LULC) datasets in the late 1970s, the early 1990s, 2004 and 2012, we analyzed characteristics of LULC change in the headwaters of the Yangtze River and Yellow River over the past 30 years contrastively, using the transition matrix and LULC change index. The results showed that, in 2012, the LULC in the headwaters of the Yellow River were different compared to those of the headwaters of the Yangtze River, with more grassland and wet- and marshland. In the past 30 years, the grassland and wet- and marshland increasing at the expense of sand, gobi, and bare land and desert were the main LULC change types in the headwaters of the Yangtze River, with the macro-ecological situation experiencing a process of degeneration, slight melioration, and continuous melioration, in that order. In the headwaters of the Yellow River, severe reduction of grassland coverage, shrinkage of wet- and marshland and the consequential expansion of sand, gobi and bare land were noticed. The macro-ecological situation experienced a process of degeneration, obvious degeneration, and slight melioration, in that order, and the overall change in magnitude was more dramatic than that in the headwaters of the Yangtze River. These different LULC change courses were jointly driven by climate change, grassland-grazing pressure, and the implementation of ecological construction projects. Full article

As the causal agent of pine wilt disease (PWD), the pine wood nematode (PWN), Bursaphelenchus xylophilus, causes huge economic losses by devastating pine forests worldwide. The pectate lyase gene is essential for successful invasion of their host plants by plant-parasitic nematodes. To demonstrate the role of pectate lyase gene in the PWD process, RNA interference (RNAi) is used to analyze the function of the pectate lyase 1 gene in B. xylophilus (Bxpel1). The efficiency of RNAi was detected by real-time PCR. The result demonstrated that the quantity of B. xylophilus propagated with control solution treatment was 62 times greater than that soaking in double-stranded RNA (dsRNA) after B. xylophilus inoculation in Botrytis cinerea for the first generation (F1). The number of B. xylophilus soaking in control solution was doubled compared to that soaking in Bxpel1 dsRNA four days after inoculation in Pinus thunbergii. The quantity of B. xylophilus was reduced significantly (p < 0.001) after treatment with dsRNAi compared with that using a control solution treatment. Bxpel1 dsRNAi reduced the migration speed and reproduction of B. xylophilus in pine trees. The pathogenicity to P. thunbergii seedling of B. xylophilus was weaker after soaking in dsRNA solution compared with that after soaking in the control solution. Our results suggest that Bxpel1 gene is a significant pathogenic factor in the PWD process and this basic information may facilitate a better understanding of the molecular mechanism of PWD. Full article

Ovarian carcinoma (OC) is one of the most common gynecological malignancies, with a poor prognosis for patients at advanced stage. Danusertib (Danu) is a pan-inhibitor of the Aurora kinases with unclear anticancer effect and underlying mechanisms in OC treatment. This study aimed to examine the cancer cell killing effect and explore the possible mechanisms with a focus on proliferation, cell cycle progression, apoptosis, autophagy, and epithelial to mesenchymal transition (EMT) in human OC cell lines C13 and A2780cp. The results showed that Danu remarkably inhibited cell proliferation, induced apoptosis and autophagy, and suppressed EMT in both cell lines. Danu arrested cells in G₂/M phase and led to an accumulation of polyploidy through the regulation of the expression key cell cycle modulators. Danu induced mitochondria-dependent apoptosis and autophagy in dose and time-dependent manners. Danu suppressed PI3K/Akt/mTOR signaling pathway, evident from the marked reduction in the phosphorylation of PI3K/Akt/mTOR, contributing to the autophagy inducing effect of Danu in both cell lines. In addition, Danu inhibited EMT. In aggregate, Danu exerts potent inducing effect on cell cycle arrest, apoptosis, and autophagy, but exhibits a marked inhibitory effect on EMT. PI3K/Akt/mTOR signaling pathway contributes, partially, to the cancer cell killing effect of Danu in C13 and A2780cp cells. Full article

Large-scale deforestation may affect the surface energy budget and consequently climate by changing the physical properties of the land surface, namely biophysical effects. This study presents the potential energy budget change caused by deforestation in Northeastern China and its climate implications, which was evaluated by quantifying the differences in MODIS-observed surface physical properties between cropland and forest. We used the MODIS land products for the period of 2001–2010 in 112 cells of 0.75° × 0.75° each, within which only best quality satellite pixels over the pure forest and cropland pixels are selected for comparison. It is estimated that cropland has a winter (summer) mean albedo of 0.38 (0.16), which is 0.15 (0.02) higher than that of forest. Due to the higher albedo, cropland absorbs 16.84 W∙m⁻² (3.08 W∙m⁻²) less shortwave radiation than forest. Compared to forest, cropland also absorbs 8.79 W∙m⁻² more longwave radiation in winter and 8.12 W∙m⁻² less longwave radiation in summer. In total, the surface net radiation of cropland is 7.53 W∙m⁻² (11.2 W∙m⁻²) less than that of forest in winter (summer). Along with these radiation changes, the latent heat flux through evapotranspiration over cropland is less than that over forest, especially in summer (−19.12 W∙m⁻²). Average sensible heat flux increases in summer (7.92 W∙m⁻²) and decreases in winter (−8.17 W∙m⁻²), suggesting that conversion of forest to cropland may lead to warming in summer and cooling in winter in Northeastern China. However, the annual net climate effect is not notable because of the opposite sign of the energy budget change in summer and winter. Full article

A large decrease in the land surface albedo of the Loess Plateau was observed from 2000 to 2010, as measured using satellite imagery. In particular, ecological restoration program regions experienced a decrease in peak season land surface albedo exceeding 0.05. In this study, we examined the spatial and temporal patterns of variation during the peak season albedo in the Loess Plateau and analyzed its relationships with changes of anthropogenic and natural factors at the pixel level. Our analysis revealed that increasing grassland coverage due to returning rangeland to grassland could lead to a maximum albedo decrease of 0.030 in peak season. This result highlighted the human-induced land use change in driving the decreasing albedo on an annual scale. There was no significant correlation between precipitation change and albedo reduction. Precipitation could influence the spatial pattern of albedo in drought years by influencing the natural vegetation water requirement. However, the role of precipitation was not obvious in the ecological restoration program regions. This article demonstrates the substantial role that land use change could play in regional-scale albedo change and climate. Finally, some implications for the radiative forcing of land use change are discussed. Full article

Bursaphelenchus xylophilus, the causal agent of pine wilt disease, causes huge economic losses in pine forests. The high expression of cytochrome P450 genes in B. xylophilus during infection in P. thunbergii indicated that these genes had a certain relationship with the pathogenic process of B. xylophilus. Thus, we attempted to identify the molecular characterization and functions of cytochrome P450 genes in B. xylophilus. In this study, full-length cDNA of three cytochrome P450 genes, BxCYP33C9, BxCYP33C4 and BxCYP33D3 were first cloned from B. xylophilus using 3' and 5' RACE PCR amplification. Sequence analysis showed that all of them contained a highly-conserved cytochrome P450 domain. The characteristics of the three putative proteins were analyzed with bioinformatic methods. RNA interference (RNAi) was used to assess the functions of BxCYP33C9, BxCYP33C4 and BxCYP33D3. The results revealed that these cytochrome P450 genes were likely to be associated with the vitality, dispersal ability, reproduction, pathogenicity and pesticide metabolism of B. xylophilus. This discovery confirmed the molecular characterization and functions of three cytochrome P450 genes from B. xylophilus and provided fundamental information in elucidating the molecular interaction mechanism between B. xylophilus and its host plant. Full article

Photoacoustic imaging (PAI) and thermoacoustic imaging (TAI) are two emerging biomedical imaging techniques that both utilize ultrasonic signals as an information carrier. Unique advantages of PAI and TAI are their abilities to provide high resolution functional information such as hemoglobin and blood oxygenation and tissue dielectric properties relevant to physiology and pathology. These two methods, however, may have a limited detection depth and lack of endogenous contrast. An exogenous contrast agent is often needed to effectively resolve these problems. Such agents are able to greatly enhance the imaging contrast and potentially break through the imaging depth limit. Furthermore, a receptor-targeted contrast agent could trace the molecular and cellular biological processes in tissues. Thus, photoacoustic and thermoacoustic molecular imaging can be outstanding tools for early diagnosis, precise lesion localization, and molecular typing of various diseases. The agents also could be used for therapy in conjugation with drugs or in photothermal therapy, where it functions as an enhancer for the integration of diagnosis and therapy. In this article, we present a detailed review about various exogenous contrast agents for photoacoustic and thermoacoustic molecular imaging. In addition, challenges and future directions of photoacoustic and thermoacoustic molecular imaging in the field of translational medicine are also discussed. Full article

The present study was designed to examine the effects of heat acclimation on enzymatic activity, transcription levels, the photosynthesis processes associated with thermostability in orchardgrass (Dactylis glomerata L.).The stomatal conductance (Gs), net photosynthetic rate (Pn), and transpiration rates (Tr) of both heat-acclimated (HA) and non-acclimated (NA) plants were drastically reduced during heat treatment [using a 5-day heat stress treatment (38/30 °C ‒ day/night) followed by a 3-day recovery under control conditions (25/20 °C ‒ day/night), in order to consolidate the second cycle was permitted]. Water use efficiency increased more steeply in the HA (4.9 times) versus the NA (1.8 times) plants, and the intercellular CO₂ concentration decreased gently in NA (10.9%) and HA (25.3%) plants after 20 d of treatments compared to 0 days’. Furthermore, heat-acclimated plants were able to maintain significant activity levels of superoxide disumutase (SOD), catalase (CAT), guaiacol peroxidase (POD), and transcription levels of genes encoding these enzymes; in addition, HA plants displayed lower malondialdehyde content and lower electrolyte leakage than NA plants. These results suggest that maintenance of activity and transcription levels of antioxidant enzymes as well as photosynthesis are associated with variable thermostability in HA and NA plants. This likely occurs through cellular membrane stabilization and improvements in water use efficiency in the photosynthetic process during heat stress. The association between antioxidant enzyme activity and gene expression, both of which may vary with genetic variation in heat tolerance, is important to further understand the molecular mechanisms that contribute to heat tolerance. Full article

The pine wood nematode, Bursaphelenchus xylophilus, is the causal agent of pine wilt disease. Accurately differentiating B. xylophilus from other nematodes species, especially its related species B. mucronatus, is important for pine wood nematode detection. Thus, we attempted to identify a specific protein in the pine wood nematode using proteomics technology. Here, we compared the proteomes of B. xylophilus and B. mucronatus using Two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization -time-of-flight/time-of-flight (MALDI-TOF/TOF-MS) technologies. In total, 15 highly expressed proteins were identified in B. xylophilus compared with B. mucronatus. Subsequently, the specificity of the proteins identified was confirmed by PCR using the genomic DNA of other nematode species. Finally, a gene encoding a specific protein (Bx-Prx) was obtained. This gene was cloned and expressed in E. coli. The in situ hybridisation pattern of Bx-Prx showed that it was expressed strongly in the tail of B. xylophilus. RNAi was used to assess the function of Bx-Prx, the results indicated that the gene was associated with the reproduction and pathogenicity of B. xylophilus. This discovery provides fundamental information for identifying B. xylophilus via a molecular approach. Moreover, the purified recombinant protein has potential as a candidate diagnostic antigen of pine wilt disease, which may lead to a new immunological detection method for the pine wood nematode. Full article