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Open AccessArticle Finite Element Computation of Transient Parameters of a Salient-Pole Synchronous Machine
Energies 2017, 10(7), 1015; doi:10.3390/en10071015
Received: 26 June 2017 / Revised: 15 July 2017 / Accepted: 16 July 2017 / Published: 17 July 2017
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Abstract
This paper presents a novel method for calculating the transient parameters of a saturated salient-pole synchronous machine (SPSM) with damper cage using finite element analysis. All detailed leakage inductances in a modified d-q model are discussed and separately extracted. In addition; the frozen
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This paper presents a novel method for calculating the transient parameters of a saturated salient-pole synchronous machine (SPSM) with damper cage using finite element analysis. All detailed leakage inductances in a modified d-q model are discussed and separately extracted. In addition; the frozen permeability method is used in a 2-D finite element analysis to consider saturation and skin effects for determining the inductances. The terminal reactance parameters are obtained from all elements of the d- and q-axis equivalent circuits in all the chosen time constants during the transient process. The variation of leakage inductances of the SPSM in transient processes is also investigated and discussed. To determine transient time constant parameters; the Prony algorithm is applied in the presented method. A program developed to automatically solve the simulation and computation with the proposed method is described. This method is applied to a prototype and validated by experimental results. Some discussion about the relationship between rotor geometric design and the transient parameters of SPSMs is provided. The variation of leakage magnetic field of SPSMs in transient processes is also investigated. This method can be applied in transient parameter estimation and optimization of SPSMs in the design stage. Full article
(This article belongs to the Section Energy Fundamentals and Conversion)
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Open AccessArticle Individual and Interactive Effects of Socio-Ecological Factors on Dengue Fever at Fine Spatial Scale: A Geographical Detector-Based Analysis
Int. J. Environ. Res. Public Health 2017, 14(7), 795; doi:10.3390/ijerph14070795
Received: 29 May 2017 / Revised: 4 July 2017 / Accepted: 12 July 2017 / Published: 17 July 2017
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Abstract
Background: Large spatial heterogeneity was observed in the dengue fever outbreak in Guangzhou in 2014, however, the underlying reasons remain unknown. We examined whether socio-ecological factors affected the spatial distribution and their interactive effects. Methods: Moran’s I was applied to first
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Background: Large spatial heterogeneity was observed in the dengue fever outbreak in Guangzhou in 2014, however, the underlying reasons remain unknown. We examined whether socio-ecological factors affected the spatial distribution and their interactive effects. Methods: Moran’s I was applied to first examine the spatial cluster of dengue fever in Guangzhou. Nine socio-ecological factors were chosen to represent the urbanization level, economy, accessibility, environment, and the weather of the 167 townships/streets in Guangzhou, and then the geographical detector was applied to analyze the individual and interactive effects of these factors on the dengue outbreak. Results: Four clusters of dengue fever were identified in Guangzhou in 2014, including one hot spot in the central area of Guangzhou and three cold spots in the suburban districts. For individual effects, the temperature (q = 0.33) was the dominant factor of dengue fever, followed by precipitation (q = 0.24), road density (q = 0.24), and water body area (q = 0.23). For the interactive effects, the combination of high precipitation, high temperature, and high road density might result in increased dengue fever incidence. Moreover, urban villages might be the dengue fever hot spots. Conclusions: Our study suggests that some socio-ecological factors might either separately or jointly influence the spatial distribution of dengue fever in Guangzhou. Full article
(This article belongs to the Section Environmental Engineering and Public Health)
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Open AccessArticle An Efficient Compensation Method for Limited-View Photoacoustic Imaging Reconstruction Based on Gerchberg–Papoulis Extrapolation
Appl. Sci. 2017, 7(5), 505; doi:10.3390/app7050505
Received: 29 March 2017 / Revised: 8 May 2017 / Accepted: 9 May 2017 / Published: 17 May 2017
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Abstract
The reconstruction for limited-view scanning, though often the case in practice, has remained a difficult issue for photoacoustic imaging (PAI). The incompleteness of sampling data will cause serious artifacts and fuzziness in those missing views and it will heavily affect the quality of
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The reconstruction for limited-view scanning, though often the case in practice, has remained a difficult issue for photoacoustic imaging (PAI). The incompleteness of sampling data will cause serious artifacts and fuzziness in those missing views and it will heavily affect the quality of the image. To solve the problem of limited-view PAI, a compensation method based on the Gerchberg–Papoulis (GP) extrapolation is applied into PAI. Based on the known data, missing detectors elements are estimated and the image in the missing views is then compensated using the Fast Fourier Transform (FFT). To accelerate the convergence speed of the algorithm, the total variation (TV)-based iterative algorithm is incorporated into the GP extrapolation-based FFT-utilized compensation method (TV-GPEF). The effective variable splitting and Barzilai–Borwein based method is adopted to solve the optimization problem. Simulations and in vitro experiments for both limited-angle circular scanning and straight-line scanning are conducted to validate the proposed algorithm. Results show that the proposed algorithm can greatly suppress the artifacts caused by the missing views and enhance the edges and the details of the image. It can be indicated that the proposed TV-GPEF algorithm is efficient for limited-view PAI. Full article
(This article belongs to the Special Issue Biomedical Photoacoustic and Thermoacoustic Imaging)
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Open AccessArticle Performance of a Novel Fertilizer-Drawn Forward Osmosis Aerobic Membrane Bioreactor (FDFO-MBR): Mitigating Salinity Build-Up by Integrating Microfiltration
Water 2017, 9(1), 21; doi:10.3390/w9010021
Received: 4 October 2016 / Revised: 14 December 2016 / Accepted: 15 December 2016 / Published: 4 January 2017
Cited by 2 | Viewed by 702 | PDF Full-text (1995 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
In this paper, three different fertilizer draw solutions were tested in a novel forward osmosis-microfiltration aerobic membrane bioreactor (MF-FDFO-MBR) hybrid system and their performance were evaluated in terms of water flux and reverse salt diffusion. Results were also compared with a standard solution.
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In this paper, three different fertilizer draw solutions were tested in a novel forward osmosis-microfiltration aerobic membrane bioreactor (MF-FDFO-MBR) hybrid system and their performance were evaluated in terms of water flux and reverse salt diffusion. Results were also compared with a standard solution. Results showed that ammonium sulfate is the most suitable fertilizer for this hybrid system since it has a relatively high water flux (6.85 LMH) with a comparatively low reverse salt flux (3.02 gMH). The performance of the process was also studied by investigating different process parameters: draw solution concentration, FO draw solution flow rate and MF imposed flux. It was found that the optimal conditions for this hybrid system were: draw solution concentration of 1 M, FO draw solution flow rate of 200 mL/min and MF imposed flux of 10 LMH. The salt accumulation increased from 834 to 5400 μS/cm during the first four weeks but after integrating MF, the salinity dropped significantly from 5400 to 1100 μS/cm suggesting that MF is efficient in mitigating the salinity build up inside the reactor. This study demonstrated that the integration of the MF membrane could effectively control the salinity and enhance the stable FO flux in the OMBR. Full article
(This article belongs to the Special Issue Advanced Membranes for Water Treatment)
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Open AccessArticle Using the Job Burden-Capital Model of Occupational Stress to Predict Depression and Well-Being among Electronic Manufacturing Service Employees in China
Int. J. Environ. Res. Public Health 2016, 13(8), 819; doi:10.3390/ijerph13080819
Received: 11 May 2016 / Revised: 28 July 2016 / Accepted: 30 July 2016 / Published: 12 August 2016
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Abstract
Background: This study aimed to identify the association between occupational stress and depression-well-being by proposing a comprehensive and flexible job burden-capital model with its corresponding hypotheses. Methods: For this research, 1618 valid samples were gathered from the electronic manufacturing service industry
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Background: This study aimed to identify the association between occupational stress and depression-well-being by proposing a comprehensive and flexible job burden-capital model with its corresponding hypotheses. Methods: For this research, 1618 valid samples were gathered from the electronic manufacturing service industry in Hunan Province, China; self-rated questionnaires were administered to participants for data collection after obtaining their written consent. The proposed model was fitted and tested through structural equation model analysis. Results: Single-factor correlation analysis results indicated that coefficients between all items and dimensions had statistical significance. The final model demonstrated satisfactory global goodness of fit (CMIN/DF = 5.37, AGFI = 0.915, NNFI = 0.945, IFI = 0.952, RMSEA = 0.052). Both the measurement and structural models showed acceptable path loadings. Job burden and capital were directly associated with depression and well-being or indirectly related to them through personality. Multi-group structural equation model analyses indicated general applicability of the proposed model to basic features of such a population. Gender, marriage and education led to differences in the relation between occupational stress and health outcomes. Conclusions: The job burden-capital model of occupational stress-depression and well-being was found to be more systematic and comprehensive than previous models. Full article
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Open AccessArticle Design and Application of Wuhan Ionospheric Oblique Backscattering Sounding System with the Addition of an Antenna Array (WIOBSS-AA)
Sensors 2016, 16(6), 887; doi:10.3390/s16060887
Received: 14 March 2016 / Revised: 8 June 2016 / Accepted: 13 June 2016 / Published: 15 June 2016
Cited by 2 | Viewed by 588 | PDF Full-text (16073 KB) | HTML Full-text | XML Full-text
Abstract
The Wuhan Ionospheric Oblique Backscattering Sounding System with the addition of an antenna array (WIOBSS-AA) is the newest member of the WIOBSS family. It is a multi-channel radio system using phased-array antenna technology. The transmitting part of this radio system applies an array
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The Wuhan Ionospheric Oblique Backscattering Sounding System with the addition of an antenna array (WIOBSS-AA) is the newest member of the WIOBSS family. It is a multi-channel radio system using phased-array antenna technology. The transmitting part of this radio system applies an array composed of five log-periodic antennas to form five beams that span an area to the northwest of the radar site. The hardware and the antenna array of the first multi-channel ionosonde in the WIOBSS family are introduced in detail in this paper. An ionospheric detection experiment was carried out in Chongyang, Hubei province, China on 16 March 2015 to examine the performance of WIOBSS-AA. The radio system demonstrated its ability to obtain ionospheric electron density information over a wide area. The observations indicate that during the experiment, the monitored large-area ionospheric F2-layer was calm and electron density increased with decreasing latitude. Full article
(This article belongs to the Section Remote Sensors)
Open AccessArticle GC×GC-TOFMS Analysis of Essential Oils Composition from Leaves, Twigs and Seeds of Cinnamomum camphora L. Presl and Their Insecticidal and Repellent Activities
Molecules 2016, 21(4), 423; doi:10.3390/molecules21040423
Received: 21 January 2016 / Revised: 18 March 2016 / Accepted: 23 March 2016 / Published: 28 March 2016
Cited by 3 | Viewed by 1046 | PDF Full-text (778 KB) | HTML Full-text | XML Full-text
Abstract
Interest in essential oils with pesticidal activity against insects and pests is growing. In this study, essential oils from different parts (leaves, twigs and seeds) of Cinnamomum camphora L. Presl were investigated for their chemical composition, and insecticidal and repellent activities against the
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Interest in essential oils with pesticidal activity against insects and pests is growing. In this study, essential oils from different parts (leaves, twigs and seeds) of Cinnamomum camphora L. Presl were investigated for their chemical composition, and insecticidal and repellent activities against the cotton aphid. The essential oils, obtained by hydrodistillation, were analyzed by GC×GC-TOFMS. A total of 96 components were identified in the essential oils and the main constituents found in the leaves and twigs were camphor, eucalyptol, linalool and 3,7-dimethyl-1,3,7-octatriene. The major components found in the seeds were eucalyptol (20.90%), methyleugenol (19.98%), linalool (14.66%) and camphor (5.5%). In the contact toxicity assay, the three essential oils of leaves, twigs and seeds exhibited a strong insecticidal activity against cotton aphids with LC50 values of 245.79, 274.99 and 146.78 mg/L (after 48 h of treatment), respectively. In the repellent assay, the highest repellent rate (89.86%) was found in the seed essential oil at the concentration of 20 μL/mL after 24 h of treatment. Linalool was found to be a significant contributor to the insecticidal and repellent activities. The results indicate that the essential oils of C. camphora might have the potential to be developed into a natural insecticide or repellent for controlling cotton aphids. Full article
(This article belongs to the Section Natural Products)
Open AccessFeature PaperArticle Influence of Nitrogen Doping on Device Operation for TiO2-Based Solid-State Dye-Sensitized Solar Cells: Photo-Physics from Materials to Devices
Nanomaterials 2016, 6(3), 35; doi:10.3390/nano6030035
Received: 19 December 2015 / Revised: 28 January 2016 / Accepted: 13 February 2016 / Published: 23 February 2016
Cited by 2 | Viewed by 1359 | PDF Full-text (2760 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Solid-state dye-sensitized solar cells (ssDSSC) constitute a major approach to photovoltaic energy conversion with efficiencies over 8% reported thanks to the rational design of efficient porous metal oxide electrodes, organic chromophores, and hole transporters. Among the various strategies used to push the performance
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Solid-state dye-sensitized solar cells (ssDSSC) constitute a major approach to photovoltaic energy conversion with efficiencies over 8% reported thanks to the rational design of efficient porous metal oxide electrodes, organic chromophores, and hole transporters. Among the various strategies used to push the performance ahead, doping of the nanocrystalline titanium dioxide (TiO2) electrode is regularly proposed to extend the photo-activity of the materials into the visible range. However, although various beneficial effects for device performance have been observed in the literature, they remain strongly dependent on the method used for the production of the metal oxide, and the influence of nitrogen atoms on charge kinetics remains unclear. To shed light on this open question, we synthesized a set of N-doped TiO2 nanopowders with various nitrogen contents, and exploited them for the fabrication of ssDSSC. Particularly, we carefully analyzed the localization of the dopants using X-ray photo-electron spectroscopy (XPS) and monitored their influence on the photo-induced charge kinetics probed both at the material and device levels. We demonstrate a strong correlation between the kinetics of photo-induced charge carriers probed both at the level of the nanopowders and at the level of working solar cells, illustrating a direct transposition of the photo-physic properties from materials to devices. Full article
(This article belongs to the Special Issue Nanostructured Solar Cells)
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Open AccessArticle Design, Synthesis and Biological Evaluation of Novel 5H-Chromenopyridines as Potential Anti-Cancer Agents
Molecules 2015, 20(9), 17152-17165; doi:10.3390/molecules200917152
Received: 20 July 2015 / Revised: 10 September 2015 / Accepted: 11 September 2015 / Published: 17 September 2015
Cited by 5 | Viewed by 1095 | PDF Full-text (889 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
A novel series of 5H-chromenopyridines was identified as anticancer agents in our continuing effort to discover and develop new small molecule anti-proliferative agents. Based on our initial lead SP-6-27 compound, we designed and synthesized novel tricyclic 5H-thiochromenopyridine and 5
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A novel series of 5H-chromenopyridines was identified as anticancer agents in our continuing effort to discover and develop new small molecule anti-proliferative agents. Based on our initial lead SP-6-27 compound, we designed and synthesized novel tricyclic 5H-thiochromenopyridine and 5H-chromenopyridine analogs to evaluate the impact of an additional ring, as well as conformational flexibility on cytotoxic activity against human melanoma and glioma cell lines. All of the 5H-thiochromenopyridines have been achieved in good yields (89%–93%) using a single-step, three-component cyclization without the need for purification. The 5H-chromenopyridine analog of the potent 5H-thiochromenopyride was obtained in a good yield upon purification. All newly-prepared 5H-thiochromenopyridines showed good to moderate cytotoxicity against three melanoma and two glioma cell lines (3–15 μM). However, the 5H-chromenopyridine analogue that we prepared in our laboratory lost cytotoxic activity. The moderate cytotoxic activity of 5H-thiochromenopyridines shows the promise of developing chromenopyridines as potential anticancer agents. Full article
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Open AccessArticle Synthesis, Larvicidal Activities and Antifungal Activities of Novel Chlorantraniliprole Derivatives and Their Target in the Ryanodine Receptor
Molecules 2015, 20(3), 3854-3867; doi:10.3390/molecules20033854
Received: 21 January 2015 / Revised: 15 February 2015 / Accepted: 24 February 2015 / Published: 2 March 2015
Cited by 3 | Viewed by 1524 | PDF Full-text (852 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
In order to identify novel chlorantraniliprole derivatives as potential insecticides or fungicides, 25 analogues of chlorantraniliprole were synthesized. The insecticidal activities against oriental armyworm and the antifungal activities against five typical fungi of these derivatives were tested. Compounds 2u, 2x and 2y
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In order to identify novel chlorantraniliprole derivatives as potential insecticides or fungicides, 25 analogues of chlorantraniliprole were synthesized. The insecticidal activities against oriental armyworm and the antifungal activities against five typical fungi of these derivatives were tested. Compounds 2u, 2x and 2y exhibited good activities against oriental armyworm, especially compounds 2u and 2x which showed higher larvicidal activities than indoxacarb. Moreover, all of the tested compounds exhibited activities against five typical fungi. The Ki values of all synthesized compounds were calculated using AutoDock4. The relationship between the Ki values and the results of insecticidal activities against oriental armyworm further indicated that the membrane-spanning domain protein of the ryanodine receptor might contain chlorantraniliprole binding sites. Full article
(This article belongs to the Section Medicinal Chemistry)
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Open AccessArticle Predicted Trans-Acting siRNAs in the Human Brain
Int. J. Mol. Sci. 2015, 16(2), 3377-3390; doi:10.3390/ijms16023377
Received: 27 October 2014 / Revised: 10 December 2014 / Accepted: 25 December 2014 / Published: 3 February 2015
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Abstract
Endogenous small non-coding RNAs play pivotal roles in regulating gene expression in eukaryotes. Many studies have investigated the function and molecular mechanism of microRNAs in the development and disease of various organisms via mRNA repression of protein-coding genes. Recent findings indicate microRNAs might
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Endogenous small non-coding RNAs play pivotal roles in regulating gene expression in eukaryotes. Many studies have investigated the function and molecular mechanism of microRNAs in the development and disease of various organisms via mRNA repression of protein-coding genes. Recent findings indicate microRNAs might trigger the generation of trans-acting small interfering RNAs (ta-siRNAs). The interaction among different types of small RNA molecules reveals an even more complicated and elaborate pattern of RNA regulation during gene expression than previously thought. We developed a method for mining ta-siRNA sequences and evaluated the performance of our novel method using data from Arabidopsis thaliana. Additionally, using small RNA and degradome data for the human brain, we identified 155 small RNAs that satisfied ta-siRNA characteristics. The DRAXIN and ATCAY genes, which are preferentially expressed in the human brain, were predicted to be the targets of 12 potential ta-siRNAs. Full article
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Open AccessArticle Antioxidant Activity of Leaf Extracts from Different Hibiscus sabdariffa Accessions and Simultaneous Determination Five Major Antioxidant Compounds by LC-Q-TOF-MS
Molecules 2014, 19(12), 21226-21238; doi:10.3390/molecules191221226
Received: 19 October 2014 / Revised: 27 November 2014 / Accepted: 12 December 2014 / Published: 17 December 2014
Cited by 12 | Viewed by 1721 | PDF Full-text (704 KB) | HTML Full-text | XML Full-text
Abstract
Hibiscus sabdariffa has gained attention for its antioxidant activity. There are many accessions of H. sabdariffa in the world. However, information on the quantification of antioxidant compounds in different accessions is rather limited. In this paper, a liquid chromatography/quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) method
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Hibiscus sabdariffa has gained attention for its antioxidant activity. There are many accessions of H. sabdariffa in the world. However, information on the quantification of antioxidant compounds in different accessions is rather limited. In this paper, a liquid chromatography/quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) method for simultaneous determination of five antioxidant compounds (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, and isoquercitrin) in H. sabdariffa leaves was developed. The method was validated for linearity, sensitivity, precision, repeatability and accuracy. The validated method has been successfully applied for determination of the five analytes in eight accessions of H. sabdariffa. The eight accessions of H. sabdariffa were evaluated for their antioxidant activities by DPPH free radical scavenging assay. The investigated accessions of H. sabdariffa were rich in rutin and exhibited strong antioxidant activity. The two accessions showing the highest antioxidant activities were from Cuba (No. 2) and Taiwan (No. 5). The results indicated that H. sabdariffa leaves could be considered as a potential antioxidant source for the food industry. The developed LC-Q-TOF-MS method is helpful for quality control of H. sabdariffa. Full article
(This article belongs to the Section Natural Products)
Open AccessArticle Towards a Fair and Efficient Packet Scheduling Scheme in Inter-Flow Network Coding
J. Sens. Actuator Netw. 2014, 3(4), 274-296; doi:10.3390/jsan3040274
Received: 23 June 2014 / Revised: 29 September 2014 / Accepted: 22 October 2014 / Published: 4 November 2014
Cited by 1 | Viewed by 1771 | PDF Full-text (251 KB) | HTML Full-text | XML Full-text
Abstract
Network coding techniques are usually applied upon network-layer protocols to improve throughput in wireless networks. In scenarios with multiple unicast sessions, fairness is also an important factor. Therefore, a network coding-aware packet-scheduling algorithm is required. A packet-scheduling algorithm determines which packet to send
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Network coding techniques are usually applied upon network-layer protocols to improve throughput in wireless networks. In scenarios with multiple unicast sessions, fairness is also an important factor. Therefore, a network coding-aware packet-scheduling algorithm is required. A packet-scheduling algorithm determines which packet to send next from a node’s packet backlog. Existing protocols mostly employ a basic round-robin scheduling algorithm to give “equal” opportunities to different packet flows. In fact, this “equal”-opportunity scheduling is neither fair, nor efficient. This paper intends to accentuate the importance of a coding-aware scheduling scheme. With a good scheduling scheme, we can gain more control over the per-flow throughput and fairness. Specifically, we first formulate a static scheduling problem and propose an algorithm to find the optimal scheduling scheme. We then extend the technique to a dynamic setting and, later, to practical routing protocols. Results show that the algorithm is comparatively scalable, and it can improve the throughput gain when the network is not severely saturated. The fairness among flows is drastically improved as a result of this scheduling scheme. Full article
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Open AccessArticle An Energy Efficient Distance-Aware Routing Algorithm with Multiple Mobile Sinks for Wireless Sensor Networks
Sensors 2014, 14(8), 15163-15181; doi:10.3390/s140815163
Received: 20 June 2014 / Revised: 7 August 2014 / Accepted: 13 August 2014 / Published: 18 August 2014
Cited by 7 | Viewed by 1766 | PDF Full-text (479 KB) | HTML Full-text | XML Full-text
Abstract
Traffic patterns in wireless sensor networks (WSNs) usually follow a many-to-one model. Sensor nodes close to static sinks will deplete their limited energy more rapidly than other sensors, since they will have more data to forward during multihop transmission. This will cause network
[...] Read more.
Traffic patterns in wireless sensor networks (WSNs) usually follow a many-to-one model. Sensor nodes close to static sinks will deplete their limited energy more rapidly than other sensors, since they will have more data to forward during multihop transmission. This will cause network partition, isolated nodes and much shortened network lifetime. Thus, how to balance energy consumption for sensor nodes is an important research issue. In recent years, exploiting sink mobility technology in WSNs has attracted much research attention because it can not only improve energy efficiency, but prolong network lifetime. In this paper, we propose an energy efficient distance-aware routing algorithm with multiple mobile sink for WSNs, where sink nodes will move with a certain speed along the network boundary to collect monitored data. We study the influence of multiple mobile sink nodes on energy consumption and network lifetime, and we mainly focus on the selection of mobile sink node number and the selection of parking positions, as well as their impact on performance metrics above. We can see that both mobile sink node number and the selection of parking position have important influence on network performance. Simulation results show that our proposed routing algorithm has better performance than traditional routing ones in terms of energy consumption. Full article
(This article belongs to the Section Sensor Networks)
Open AccessArticle Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44
Int. J. Mol. Sci. 2014, 15(5), 8473-8490; doi:10.3390/ijms15058473
Received: 22 March 2014 / Revised: 5 May 2014 / Accepted: 5 May 2014 / Published: 13 May 2014
Cited by 7 | Viewed by 2296 | PDF Full-text (798 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Abscisic acid (ABA) signaling plays important roles in plant growth, development and adaptation to various stresses. RCAR1/PYL9 has been known as a cytoplasm and nuclear ABA receptor in Arabidopsis. To obtain further insight into the regulatory mechanism of RCAR1/PYL9, a yeast two-hybrid
[...] Read more.
Abscisic acid (ABA) signaling plays important roles in plant growth, development and adaptation to various stresses. RCAR1/PYL9 has been known as a cytoplasm and nuclear ABA receptor in Arabidopsis. To obtain further insight into the regulatory mechanism of RCAR1/PYL9, a yeast two-hybrid approach was performed to screen for RCAR1/PYL9-interacting proteins and an R2R3-type MYB transcription factor, AtMYB44, was identified. The interaction between RCAR1/PYL9 and AtMYB44 was further confirmed by glutathione S-transferase (GST) pull-down and bimolecular fluorescence complementation (BiFC) assays. Gene expression analysis showed that AtMYB44 negatively regulated the expression of ABA-responsive gene RAB18, in contrast to the opposite role reported for RCAR1/PYL9. Competitive GST pull-down assay and analysis of phosphatase activity demonstrated that AtMYB44 and ABI1 competed for binding to RCAR1/PYL9 and thereby reduced the inhibitory effect of RCAR1/PYL9 on ABI1 phosphatase activity in the presence of ABA in vitro. Furthermore, transient activation assay in protoplasts revealed AtMYB44 probably also decreased RCAR1/PYL9-mediated inhibition of ABI1 activity in vivo. Taken together, our work provides a reasonable molecular mechanism of AtMYB44 in ABA signaling. Full article
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
Open AccessReview Tumor-Associated Circulating MicroRNAs as Biomarkers of Cancer
Molecules 2014, 19(2), 1912-1938; doi:10.3390/molecules19021912
Received: 10 December 2013 / Revised: 24 January 2014 / Accepted: 29 January 2014 / Published: 10 February 2014
Cited by 69 | Viewed by 4100 | PDF Full-text (308 KB) | HTML Full-text | XML Full-text
Abstract
MicroRNAs (miRNAs), the 17- to 25-nucleotide long noncoding RNAs that modulate the expression of mRNAs and proteins, have emerged as critical players in cancer initiation and progression processes. Deregulation of tissue miRNA expression levels associated with specific genetic alterations has been demonstrated in
[...] Read more.
MicroRNAs (miRNAs), the 17- to 25-nucleotide long noncoding RNAs that modulate the expression of mRNAs and proteins, have emerged as critical players in cancer initiation and progression processes. Deregulation of tissue miRNA expression levels associated with specific genetic alterations has been demonstrated in cancer, where miRNAs function either as oncogenes or as tumor-suppressor genes and are shed from cancer cells into circulation. The present review summarizes and evaluates recent advances in our understanding of the characteristics of tumor tissue miRNAs, circulating miRNAs, and the stability of miRNAs in tissues and their varying expression profiles in circulating tumor cells, and body fluids including blood plasma. These advances in knowledge have led to intense efforts towards discovery and validation of differentially expressing tumor-associated miRNAs as biomarkers and therapeutic targets of cancer. The development of tumor-specific miRNA signatures as cancer biomarkers detectable in malignant cells and body fluids should help with early detection and more effective therapeutic intervention for individual patients. Full article
(This article belongs to the Special Issue miRNAs as Probes to Monitor Cancer and Neurodegenerative Disorders)
Open AccessArticle DNA Repair Gene XRCC4 Codon 247 Polymorphism Modified Diffusely Infiltrating Astrocytoma Risk and Prognosis
Int. J. Mol. Sci. 2014, 15(1), 250-260; doi:10.3390/ijms15010250
Received: 28 October 2013 / Revised: 1 December 2013 / Accepted: 23 December 2013 / Published: 27 December 2013
Cited by 7 | Viewed by 1591 | PDF Full-text (348 KB) | HTML Full-text | XML Full-text
Abstract
The DNA repair gene X-ray cross-complementary group 4 (XRCC4), an important caretaker of the overall genome stability, is thought to play a major role in human tumorigenesis. We investigated the association between an important polymorphic variant of this gene at codon 247 (rs373409)
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The DNA repair gene X-ray cross-complementary group 4 (XRCC4), an important caretaker of the overall genome stability, is thought to play a major role in human tumorigenesis. We investigated the association between an important polymorphic variant of this gene at codon 247 (rs373409) and diffusely infiltrating astrocytoma (DIA) risk and prognosis. This hospital-based case-control study investigated this association in the Guangxi population. In total, 242 cases with DIA and 358 age-, sex-, and race-matched healthy controls were genotyped using TaqMan-PCR technique. We found a significant difference in the frequency of XRCC4 genotypes between cases and controls. Compared with the homozygote of XRCC4 codon 247 Ala alleles (XRCC4-AA), the genotypes of XRCC4 codon 247 Ser alleles (namely XRCC4-AS or -SS) increased DIA risk (odds ratios [OR], 1.82 and 2.89, respectively). Furthermore, XRCC4 polymorphism was correlated with tumor dedifferentiation of DIA (r = 0.261, p < 0.01). Additionally, this polymorphism modified the overall survival of DIA patients (the median survival times were 26, 14, and 8 months for patients with XRCC4-AA, -AS, and -SS, respectively). Like tumor grade, XRCC4 codon 247 polymorphism was an independent prognostic factor influencing the survival of DIA. These results suggest that XRCC4 codon 247 polymorphism may be associated with DIA risk and prognosis among the Guangxi population. Full article
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Open AccessArticle Block-to-Point Fine Registration in Terrestrial Laser Scanning
Remote Sens. 2013, 5(12), 6921-6937; doi:10.3390/rs5126921
Received: 23 October 2013 / Revised: 3 December 2013 / Accepted: 5 December 2013 / Published: 11 December 2013
Cited by 5 | Viewed by 1546 | PDF Full-text (1659 KB) | HTML Full-text | XML Full-text
Abstract
Fine registration of point clouds plays an important role in data analysis in Terrestrial Laser Scanning (TLS). This work proposes a block-to-point fine registration approach to correct the errors of point clouds from TLS and of geodetic networks observed using total stations. Based
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Fine registration of point clouds plays an important role in data analysis in Terrestrial Laser Scanning (TLS). This work proposes a block-to-point fine registration approach to correct the errors of point clouds from TLS and of geodetic networks observed using total stations. Based on a reference coordinate system, the block-to-point estimation is performed to obtain representative points. Then, fine registration with a six-parameter transformation is performed with the help of an Iterative Closest Point (ICP) method. For comparisons, fine registration with a seven-parameter transformation is introduced by applying a Singular Value Decomposition (SVD) algorithm. The proposed method not only corrects the registration errors between a geodetic network and the scans, but also considers the errors among the scans. The proposed method was tested on real TLS data of a dam surface, and the results showed that distance discrepancies of estimated representative points between scans were reduced by approximately 60%. Full article
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Open AccessArticle Harmonic Loss Analysis of the Traction Transformer of High-Speed Trains Considering Pantograph-OCS Electrical Contact Properties
Energies 2013, 6(11), 5826-5846; doi:10.3390/en6115826
Received: 22 August 2013 / Revised: 31 October 2013 / Accepted: 31 October 2013 / Published: 7 November 2013
Cited by 2 | Viewed by 1904 | PDF Full-text (450 KB) | HTML Full-text | XML Full-text
Abstract
The traction transformer of the traction drive system of a high-speed train is one of the main equipments for energy conversion. The transformer loss will be increased by load harmonics and pantograph arcs at high speed. It is very important to predict losses
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The traction transformer of the traction drive system of a high-speed train is one of the main equipments for energy conversion. The transformer loss will be increased by load harmonics and pantograph arcs at high speed. It is very important to predict losses for the improvement of traction transformer design. In this paper, a dynamic model of the pantograph-catenary system is established using the MSC.Marc software based on the finite element method to analyze disconnection events in different speeds. Then the pantograph arc, traction transformer and four-quadrant converter model is set up. Resistance variations with the change of harmonic frequency have been considered in the calculation formulae of harmonic losses. Traction transformer losses can be calculated based on the harmonic T-equivalent circuit and superposition principle. Considering the harmonic losses variations, the effects of arc voltage on harmonic copper loss and harmonic core loss are analyzed, respectively. The average loss at different disconnection ratios is also calculated. This method could be used to estimate the increment of transformer harmonic losses with poor current conditions at high speed. Full article
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Open AccessArticle An Energy Efficient Stable Election-Based Routing Algorithm for Wireless Sensor Networks
Sensors 2013, 13(11), 14301-14320; doi:10.3390/s131114301
Received: 9 September 2013 / Revised: 18 October 2013 / Accepted: 18 October 2013 / Published: 24 October 2013
Cited by 19 | Viewed by 2441 | PDF Full-text (336 KB) | HTML Full-text | XML Full-text
Abstract
Sensor nodes usually have limited energy supply and they are impractical to recharge. How to balance traffic load in sensors in order to increase network lifetime is a very challenging research issue. Many clustering algorithms have been proposed recently for wireless sensor networks
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Sensor nodes usually have limited energy supply and they are impractical to recharge. How to balance traffic load in sensors in order to increase network lifetime is a very challenging research issue. Many clustering algorithms have been proposed recently for wireless sensor networks (WSNs). However, sensor networks with one fixed sink node often suffer from a hot spots problem since nodes near sinks have more traffic burden to forward during a multi-hop transmission process. The use of mobile sinks has been shown to be an effective technique to enhance network performance features such as latency, energy efficiency, network lifetime, etc. In this paper, a modified Stable Election Protocol (SEP), which employs a mobile sink, has been proposed for WSNs with non-uniform node distribution. The decision of selecting cluster heads by the sink is based on the minimization of the associated additional energy and residual energy at each node. Besides, the cluster head selects the shortest path to reach the sink between the direct approach and the indirect approach with the use of the nearest cluster head. Simulation results demonstrate that our algorithm has better performance than traditional routing algorithms, such as LEACH and SEP. Full article
(This article belongs to the Section Sensor Networks)
Open AccessArticle Sexual and Reproductive Health among Unmarried Rural-Urban Female Migrants in Shanghai China: A Comparative Analysis
Int. J. Environ. Res. Public Health 2013, 10(8), 3578-3589; doi:10.3390/ijerph10083578
Received: 24 May 2013 / Revised: 12 July 2013 / Accepted: 31 July 2013 / Published: 9 August 2013
Cited by 3 | Viewed by 1918 | PDF Full-text (236 KB) | HTML Full-text | XML Full-text
Abstract
We compared sexual and reproductive health (SRH)-related knowledge, attitude and behavior among unmarried rural-urban female migrants in Shanghai coming from different regions of China. A total of 944 unmarried rural-urban female migrants were recruited from three districts of Shanghai. We used an interviewer-administered
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We compared sexual and reproductive health (SRH)-related knowledge, attitude and behavior among unmarried rural-urban female migrants in Shanghai coming from different regions of China. A total of 944 unmarried rural-urban female migrants were recruited from three districts of Shanghai. We used an interviewer-administered structured questionnaire to collect information from each participant and a multivariate logistic regression to examine the association between premarital sex and risk factors. We found the rates of premarital sex, pregnancy and abortion among unmarried rural-urban female migrants were 28.2%, 5.2% and 5.0%, respectively. Participants from the east of China were more likely to engage in premarital sex than those from the mid-west (p < 0.001). The analysis showed premarital sex was associated with age, hometown, education, current residential type, knowledge of sexual physiology and safe sex, attitude to SRH and safe sex, and permissive attitude to sex. Unmarried rural-urban female migrants lack SRH related knowledge and the data suggests high levels of occurrence of premarital sex. The results indicate that programs to promote safe sex, especially to those migrants coming from eastern China, should be a priority. Full article
Open AccessArticle Cascading cis-Cleavage on Transcript from trans-Acting siRNA-Producing Locus 3
Int. J. Mol. Sci. 2013, 14(7), 14689-14699; doi:10.3390/ijms140714689
Received: 2 May 2013 / Revised: 24 June 2013 / Accepted: 4 July 2013 / Published: 12 July 2013
Cited by 8 | Viewed by 2678 | PDF Full-text (642 KB) | HTML Full-text | XML Full-text
Abstract
The production of small RNAs (sRNAs) from phased positions set by microRNA-directed cleavage of trans-acting-siRNA-producing locus (TAS) transcript has been characterized extensively; however, the production of sRNAs from non-phased positions remains unknown. We report three cis-cleavages that occurred in TAS3 transcripts
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The production of small RNAs (sRNAs) from phased positions set by microRNA-directed cleavage of trans-acting-siRNA-producing locus (TAS) transcript has been characterized extensively; however, the production of sRNAs from non-phased positions remains unknown. We report three cis-cleavages that occurred in TAS3 transcripts in Vitis vinifera, by combining high-throughput sRNA deep sequencing information with evolutional conservation and genome-wide RNA degradome analysis. The three cis-cleavages can be deciphered to generate an orderly cleavage cascade, and can also produce distinct phasing patterns. Each of the patterns, either upstream or downstream of the cis-cleaved position, had a set of sRNAs arranged in 21-nucleotide increments. Part of the cascading cis-cleavages was also conserved in Arabidopsis thaliana. Our results will enhance the understanding of the production of sRNAs from non-phased positions that are not set by microRNA-directed cleavage. Full article
(This article belongs to the Special Issue Regulation by non-coding RNAs 2013) Printed Edition available
Open AccessArticle Protective Effect of Ginsenoside Rb1 Against Lung Injury Induced by Intestinal Ischemia-Reperfusion in Rats
Molecules 2013, 18(1), 1214-1226; doi:10.3390/molecules18011214
Received: 13 December 2012 / Revised: 5 January 2013 / Accepted: 11 January 2013 / Published: 17 January 2013
Cited by 16 | Viewed by 2195 | PDF Full-text (988 KB)
Abstract
Intestinal ischemia-reperfusion (I/R) is a critical event in the pathogenesis of multiple organ dysfunction syndromes (MODS). The lungs are some of the most vulnerable organs that are impacted by intestinal I/R. The aim of this study is to investigate whether ginsenoside Rb1 can
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Intestinal ischemia-reperfusion (I/R) is a critical event in the pathogenesis of multiple organ dysfunction syndromes (MODS). The lungs are some of the most vulnerable organs that are impacted by intestinal I/R. The aim of this study is to investigate whether ginsenoside Rb1 can ameliorate remote lung injury induced by intestinal I/R. Adult male Wistar rats were randomly divided into four groups: (1) a control, sham-operated group (sham group); (2) an intestinal I/R group subjected to 1 h intestinal ischemia and 2 h reperfusion (I/R group); (3) a group treated with 20 mg/kg ginsenoside Rb1 before reperfusion (Rb1-20 group); and (4) a group treated with 40 mg/kg ginsenoside Rb1 before reperfusion (Rb1-40 group). Intestinal and lung histology was observed. The malondialdehyde (MDA) levels in intestinal tissues were measured. Myeloperoxidase (MPO), TNF-α, MDA levels, wet/dry weight ratio and immunohistochemical expression of intracellular adhesion molecule-1 (ICAM-1) in lung tissues were assayed. In addition, a western blot of lung NF-kB was performed. Results indicated that intestinal I/R induced intestinal and lung injury, which was characterized by increase of MDA levels and pathological scores in intestinal tissues and MPO, TNF-α , MDA levels, wet/dry weight ratio and ICAM-1, NF-kB expression in the lung tissues. Ginsenoside Rb1 (20, 40 mg/kg) ameliorated intestinal and lung injury, decreased MPO, TNF-α, MDA levels, wet/dry weight ratio, ICAM-1 and NF-kB expression in lung tissues. In conclusion, ginsenoside Rb1 ameliorated the lung injuries by decreasing the NF-kB activation-induced inflammatory response. Full article
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Open AccessArticle TLC Screening for Antioxidant Activity of Extracts from Fifteen Bamboo Species and Identification of Antioxidant Flavone Glycosides from Leaves of Bambusa. textilis McClure
Molecules 2012, 17(10), 12297-12311; doi:10.3390/molecules171012297
Received: 10 September 2012 / Revised: 19 September 2012 / Accepted: 12 October 2012 / Published: 19 October 2012
Cited by 19 | Viewed by 2782 | PDF Full-text (317 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Interest in the antioxidant activity of bamboo leaves is growing. To discover new sources of natural antioxidants, a TLC bioautography method combined with a new image processing method was developed to evaluate the antioxidant activity of leaf extracts from 15 different species of
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Interest in the antioxidant activity of bamboo leaves is growing. To discover new sources of natural antioxidants, a TLC bioautography method combined with a new image processing method was developed to evaluate the antioxidant activity of leaf extracts from 15 different species of bamboo. The results showed that the methanolic extract of Bambusa. textilis McClure possessed the highest antioxidant activity among the selected bamboo species. To rapidly identify the antioxidant compounds, the crude extract of B. textilis McClure was analysed by HPLC-UV, and HPLC-micro-fractionation of the extract was carried out. Based on TLC bioautography-guided fractionation, three antioxidant fractions were isolated from B. textilis McClure by preparative chromatography. These three antioxidant compounds were identified as isoorientin 4''-O β-D-xylopyranoside (1), isoorientin 2''-O-α-L-rhamnoside (2) and isoorientin (3) according to their UV, MS, and NMR data. The proposed TLC screening method could therefore be an easy way to evaluate the antioxidant activity of bamboo leaves, and the results achieved should prove very helpful for promoting their utilization, as B. textilis McClure can be considered a promising plant source of natural antioxidants. Full article
(This article belongs to the Special Issue Antioxidants 2012)
Open AccessArticle Screening and Analysis of the Potential Bioactive Components in Rabbit Plasma after Oral Administration of Hot-Water Extracts from Leaves of Bambusa textilis McClure
Molecules 2012, 17(8), 8872-8885; doi:10.3390/molecules17088872
Received: 8 June 2012 / Revised: 17 July 2012 / Accepted: 18 July 2012 / Published: 26 July 2012
Cited by 7 | Viewed by 2473 | PDF Full-text (243 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Bambusa textilis McClure is a traditional Chinese medicinal plant belonging to the Bambusoideae subfamily and used to treat chronic fever and infectious diseases. To investigate the bioactive compounds absorbed in the rabbit blood after oral administration of hot-water extracts from the
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Bambusa textilis McClure is a traditional Chinese medicinal plant belonging to the Bambusoideae subfamily and used to treat chronic fever and infectious diseases. To investigate the bioactive compounds absorbed in the rabbit blood after oral administration of hot-water extracts from the leaves of B. textilis McClure, a validated chromatographic fingerprint method was established using LC-Q-TOF-MS. Twenty compounds in bamboo leaves and three potential bioactive compounds in rabbit plasma were detected. Of the twenty detected compounds in vitro, fifteen of which were tentatively identified either by comparing the retention time and mass spectrometry data with that of reference compounds or by reviewing the literature. Three potential bioactive compounds, including (E)-p-coumaric acid, (Z)-p-coumaric acid, and apigenin-8-C-β-D-(2"-O-α-L-rhamnosyl)-gluco-pyranoside, were detected in both the leaves of B. textilis McClure and rabbit plasma. Of the three compounds, apigenin-8-C-β-D-(2"-O-α-L-rhamnosyl)glucopyranoside was identified based on its UV, MS, and NMR spectra. This study provides helpful chemical information for further pharmacology and active mechanism research on B. textilis McClure. Full article
(This article belongs to the Section Natural Products)
Open AccessArticle Robust Feedback Zoom Tracking for Digital Video Surveillance
Sensors 2012, 12(6), 8073-8099; doi:10.3390/s120608073
Received: 9 April 2012 / Revised: 31 May 2012 / Accepted: 1 June 2012 / Published: 11 June 2012
Cited by 3 | Viewed by 2450 | PDF Full-text (3480 KB) | HTML Full-text | XML Full-text
Abstract
Zoom tracking is an important function in video surveillance, particularly in traffic management and security monitoring. It involves keeping an object of interest in focus during the zoom operation. Zoom tracking is typically achieved by moving the zoom and focus motors in lenses
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Zoom tracking is an important function in video surveillance, particularly in traffic management and security monitoring. It involves keeping an object of interest in focus during the zoom operation. Zoom tracking is typically achieved by moving the zoom and focus motors in lenses following the so-called “trace curve”, which shows the in-focus motor positions versus the zoom motor positions for a specific object distance. The main task of a zoom tracking approach is to accurately estimate the trace curve for the specified object. Because a proportional integral derivative (PID) controller has historically been considered to be the best controller in the absence of knowledge of the underlying process and its high-quality performance in motor control, in this paper, we propose a novel feedback zoom tracking (FZT) approach based on the geometric trace curve estimation and PID feedback controller. The performance of this approach is compared with existing zoom tracking methods in digital video surveillance. The real-time implementation results obtained on an actual digital video platform indicate that the developed FZT approach not only solves the traditional one-to-many mapping problem without pre-training but also improves the robustness for tracking moving or switching objects which is the key challenge in video surveillance. Full article
(This article belongs to the Section Physical Sensors)
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Open AccessArticle A Distance-Based Energy Aware Routing Algorithm for Wireless Sensor Networks
Sensors 2010, 10(10), 9493-9511; doi:10.3390/s101009493
Received: 4 August 2010 / Revised: 20 September 2010 / Accepted: 19 October 2010 / Published: 21 October 2010
Cited by 14 | Viewed by 6108 | PDF Full-text (389 KB) | HTML Full-text | XML Full-text
Abstract
Energy efficiency and balancing is one of the primary challenges for wireless sensor networks (WSNs) since the tiny sensor nodes cannot be easily recharged once they are deployed. Up to now, many energy efficient routing algorithms or protocols have been proposed with techniques
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Energy efficiency and balancing is one of the primary challenges for wireless sensor networks (WSNs) since the tiny sensor nodes cannot be easily recharged once they are deployed. Up to now, many energy efficient routing algorithms or protocols have been proposed with techniques like clustering, data aggregation and location tracking etc. However, many of them aim to minimize parameters like total energy consumption, latency etc., which cause hotspot nodes and partitioned network due to the overuse of certain nodes. In this paper, a Distance-based Energy Aware Routing (DEAR) algorithm is proposed to ensure energy efficiency and energy balancing based on theoretical analysis of different energy and traffic models. During the routing process, we consider individual distance as the primary parameter in order to adjust and equalize the energy consumption among involved sensors. The residual energy is also considered as a secondary factor. In this way, all the intermediate nodes will consume their energy at similar rate, which maximizes network lifetime. Simulation results show that the DEAR algorithm can reduce and balance the energy consumption for all sensor nodes so network lifetime is greatly prolonged compared to other routing algorithms. Full article
Open AccessArticle Micro Semiconductor CO Sensors Based on Indium-Doped Tin Dioxide Nanocrystalline Powders
Sensors 2006, 6(5), 526-535; doi:10.3390/s6050526
Received: 10 March 2006 / Accepted: 10 May 2006 / Published: 10 May 2006
Cited by 9 | Viewed by 6354 | PDF Full-text (436 KB) | HTML Full-text | XML Full-text
Abstract
The precursors of SnO2 or In2O3/SnO2 nanocrystlline powders have been prepared bythe sol-precipitation method. The precursors were calcined at different temperatures to prepareSnO2 or In2O3/SnO2 nanocrystalline powders with different particle sizes.
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The precursors of SnO2 or In2O3/SnO2 nanocrystlline powders have been prepared bythe sol-precipitation method. The precursors were calcined at different temperatures to prepareSnO2 or In2O3/SnO2 nanocrystalline powders with different particle sizes. The nanocrystalliteswere examined by differential thermal analysis (DTA), X-ray diffraction (XRD) andtransmission electron microscopy (TEM). And then thick film CO sensors were fabricated usingprepared SnO2 or In2O3/SnO2 nanocrystlline powders loaded with PdOx. The composition thatgave the highest sensitivity for CO was in the weight% ratio of 5 wt.% In2O3/SnO2:PdOx as99:1(wt %). The composite material was found sensitive against CO at the working temperature200 °C. It was found that the sensors based on In2O3/SnO2 nanocrystalline system exhibitedvery short response time to CO at ppm level. These characteristics make the sensor to be apromising candidate for detecting low concentrations of CO. Full article
(This article belongs to the Special Issue Gas Sensors)

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