Authors: Danna Carolina Arenas Eljach Javier Hernando Albarracin Navas Luz Zoraya Beatriz Duarte Rodriguez Daniel Leonardo Cala-Delgado
Persistent Müllerian duct syndrome (PMDS) is a rare disorder of sex development characterized by the persistence of Müllerian duct derivatives in genetically and phenotypically male animals. This report describes the clinical presentation, diagnostic work-up, surgical management, histopathological findings, and cytogenetic characterization of a 9-year-old intact male Schnauzer dog presented with a 3-day history of progressively worsening vomiting, diarrhea, unilateral testicular enlargement, oliguria, and dysuria. Diagnostic evaluation included abdominal radiography, ultrasonography, exploratory laparotomy, histopathology, and cytogenetic analysis using R-replicative banding after bromodeoxyuridine incorporation. Imaging and surgical exploration revealed cryptorchidism, persistent Müllerian duct derivatives, including uterine horn-like structures, and an abnormal gonadal structure. Histopathological examination confirmed severe seminiferous tubular atrophy, persistence of Müllerian-derived tissues, and a unilateral Sertoli cell tumor. Cytogenetic analysis demonstrated a normal male karyotype (78,XY) without evidence of chromosomal mosaicism or structural abnormalities, supporting the diagnosis of a 78,XY disorder of sex development and excluding major chromosomal defects. This case highlights the importance of integrating clinical evaluation, diagnostic imaging, histopathology, and cytogenetic analysis to identify findings compatible with PMDS and emphasizes that this condition should be considered in the differential diagnosis of cryptorchid male dogs presenting with reproductive tract abnormalities or associated testicular neoplasia.
]]>Authors: Amanda N. Samuels Véronique A. Lacombe
Donkeys and horses diverged approximately 4 million years ago and exhibit substantial genetic, chromosomal, and physiologic differences. Although mostly studied in horses, metabolic syndrome represents a broader metabolic dysfunction with systemic consequences, such as obesity and laminitis, that can impact the quality of life of equids. This review summarizes the current knowledge of glucose and insulin regulation in healthy horses and donkeys and examines the pathophysiology of equine metabolic syndrome (EMS) in comparison with donkey metabolic syndrome (DMS), including insulin dysregulation, impaired glucose transport, adipose tissue dysfunction, and emerging evidence of systemic meta-inflammation and oxidative stress. Compared with horses, donkeys demonstrate distinct patterns of adipose deposition, different metabolic profiles, and altered glucose and insulin dynamics following oral soluble carbohydrate intake. While our understanding of donkey medicine has expanded in recent years, gaps in knowledge remain regarding mechanistic insights into glucose metabolism and dysregulation in donkeys. Finally, we evaluate current and emerging therapeutic strategies, including dietary management, exercise, nutraceuticals, and pharmacologic interventions such as metformin and sodium–glucose cotransporter 2 inhibitors (SGLT2is). While these therapeutic approaches have demonstrated efficacy in horses, evidence in donkeys remains extremely limited. This highlights a critical need for species-specific research to elucidate the pathophysiological mechanisms and optimize therapeutic strategies for donkeys.
]]>Authors: Laura V. Holmes Joseph Cyrus Parambeth Lisa J. Thompson
(1) Background: Migrating grass awns can cause significant inflammation and tissue damage. Endoscopy is well known to be an effective tool for both diagnosis and treatment of respiratory tract and upper gastrointestinal foreign bodies; however, its safety and efficacy for the removal of grass awns has not been evaluated. This study aims to describe the characteristics of dogs affected by grass awn foreign bodies, identify common sites of awn embedment, describe the outcomes of endoscopic procedures, and identify any complications associated with endoscopic removal of grass awn foreign bodies from the nasal cavity and gastrointestinal (GI) tract. Endoscopy is hypothesized to be a safe and effective means for both diagnosis and removal of migrating grass awns from the nasal cavity and upper GI tract. (2) Methods: This is a retrospective observational case series of 18 client-owned dogs undergoing endoscopy for removal of grass awns in the nasal cavity or gastrointestinal tract. Cases for inclusion were identified by reviewing the medical records of all animals that underwent rhinoscopy or endoscopy for suspected foreign bodies. Cases were included if one or more grass awns were identified on endoscopy. (3) Results: Most grass awns were found in the nasal cavity, tonsillar crypts, and at the upper esophageal sphincter, each representing 23% of identified awns. Sneezing and nasal discharge were correlated with awns in the nasal cavity, whereas other clinical signs were less indicative of awn location. Seventy-two percent of cases (13/18) had complete removal of all visible awns, while 28% (5/18) had incomplete removal. Long-term follow-up was limited; however, all dogs with follow-up data available showed resolution of clinical signs. No significant endoscopic complications were reported. (4) Conclusions: Endoscopy appears to be a safe and effective diagnostic and therapeutic modality for the identification and removal of grass awns from the nasal cavity and upper GI tract. Clinical signs may help predict awn location and guide endoscopic evaluation. Additional studies with standardized follow-up are needed to better define clinical outcomes and factors associated with successful retrieval.
]]>Authors: Vincenzo Tufarelli
Chronic kidney disease (CKD) is common in older cats, and nutritional management remains the intervention with the strongest clinical evidence. Interest has expanded from conventional control of phosphorus and uremic signs to the gut–kidney axis, in which renal dysfunction may alter the intestinal environment while microbial metabolism generates solutes that accumulate as kidney function declines. This PRISMA-ScR-guided scoping review and critical narrative synthesis evaluates feline evidence on CKD-associated dysbiosis, gut-derived uremic solutes, bile acid metabolism, and microbiome-directed nutrition. The original search covered January 2021 to April 2026, with inclusion of earlier studies and targeted source verification through July 2026. Eligibility was organized into two evidence strata: core feline evidence and contextual evidence. The final revised evidence map comprised 49 unique sources (28 empirical feline sources and 21 contextual sources spanning comparative, methodological, guideline, regulatory, or safety evidence); non-feline evidence was used only for mechanistic, methodological, safety, or translational interpretation and was not pooled with feline clinical outcomes. Cats with CKD have shown lower fecal microbial richness and diversity and higher circulating indoxyl sulfate in small cross-sectional cohorts, but causality remains unproven. Recent work also identified altered secondary bile acids and lower fecal ursodeoxycholic acid; however, Peptacetobacter hiranonis is principally linked to bai-mediated 7α-dehydroxylation, whereas ursodeoxycholic acid formation requires distinct hydroxysteroid dehydrogenase reactions. Complete therapeutic renal diets improve clinical outcomes, but their benefits reflect multiple simultaneous modifications and cannot yet be decomposed into a microbiome-specific effect. Evidence for isolated prebiotics, probiotics, postbiotics, synbiotics, fecal microbiota transplantation, and precision nutrition algorithms is preliminary or absent in feline CKD. The field is constrained by small cohorts, confounding, reliance on 16S rRNA sequencing, compositional data, and limited interlaboratory reproducibility. Current clinical practice should therefore prioritize a palatable complete renal diet, adequate energy and protein intake, muscle condition monitoring, hydration, and constipation management, while microbiome-directed products remain adjunctive or investigational.
]]>Authors: Laura Ann Reese
Rationale: This research explores the connections between shelter and rescue revenues and expenses and live release outcomes, specifically focusing on the implications of a nonprofit having a government contract to provide animal control and welfare services. Methods: Two data sources were used for the project: nonprofit I990s as reported to the Internal Revenue Service (for financial data) and live release data from Shelter Animals Count (to measure shelter outcomes). The project focuses on financing for nonprofit shelters and rescues and 252 organizations from the Shelter Animals Count database, classified as nonprofits with and without government contracts, were used in the analysis. Data on revenue and expenditures were pulled from the nonprofit 990s using the GuideStar website. Results: Two variables—relinquishment rate and diversification of revenue—account for 40% of the variation in live release rates. Organizations with greater diversification of revenue have significantly higher live release rates and those with higher numbers of relinquished animals have lower ones. Conclusions: The study concludes that: a more even distribution of revenue between donations, programs, and government contracts for service is related to better outcomes; it is not so much whether a shelter or rescue has a government contract, but the portion of its revenue provided by the contract that is related to live release rates; and, overall, there are few significant relationships between revenue, expenditures, and outcomes.
]]>Authors: Epameinondas Loukopoulos Vasiliki Siatra Leonidas Folias Panagiota Tyrnenopoulou Konstantina Karagianni Mandalena Markou Vassiliki Tsioli Eugenia Flouraki
Locoregional anaesthesia is vital in modern equine veterinary practice. It effectively manages acute pain and may reduce the anaesthetic requirements and adverse effects associated with general anaesthesia. This review summarises the currently described local anaesthetic techniques used in horses, with emphasis on epidural approaches, peripheral nerve blocks, and fascial plane techniques. The relevant literature was identified through searches of PubMed, Web of Science, and Scopus databases. The review discusses the pharmacological properties of commonly used local anaesthetics, differences between short- and long-acting agents, and the potential role of adjuvants in prolonging analgesia. In addition, the available evidence regarding ultrasound-guided and nerve stimulator-guided techniques is presented, including their applications in thoracic, abdominal, and reproductive procedures. Attention is given to recently described fascial plane blocks and to the growing use of ultrasound guidance for improving anatomical identification and needle placement. The available evidence suggests that locoregional anaesthesia can contribute to multimodal analgesia in horses while potentially reducing perioperative systemic analgesic requirements. However, the clinical evidence supporting several techniques remains limited, and further controlled studies are required to evaluate their efficacy, safety, and reproducibility under clinical conditions.
]]>Authors: Sara Crimi Davide Danieli Graziana Gambino Michela Amadori Cristina Vercelli Giovanni Re
Skin diseases are among the most frequently diagnosed conditions in companion animals and have historically been treated empirically with antibiotics, contributing to the emergence and persistence of antimicrobial resistance (AMR). In recent years, greater awareness of AMR has led to a reduction in antibiotic use in veterinary medicine, particularly in dermatology. To assess AMR trends in skin infections and evaluate the use of diagnostic tools to guide antimicrobial prescriptions, we conducted a retrospective study of medical records from a veterinary hospital in northwestern Italy between 2013 and 2024. A total of 447 antimicrobial susceptibility tests from dogs and cats with clinical signs of skin disease were analyzed. Of these, 391 yielded positive bacterial cultures, and 56 were negative. Most positive samples came from dogs (83.4%), with cats accounting for 16.6%. The most frequently isolated bacterial families were Staphylococcaceae, Enterobacteriaceae, and Pseudomonadaceae. Amoxicillin-clavulanic acid was prescribed in 36.6% of patients, fluoroquinolones in 25.9%, while 17% received only detergent–disinfectant products. Among first-line drugs, ampicillin showed the lowest susceptibility, with resistance exceeding 60%, whereas imipenem (used as an indicator of carbapenemase production but avoided in veterinary medicine) retained high activity, with susceptibility rates of 77.2% in dogs and 90.3% in cats. These findings are clinically relevant, but continuous AMR surveillance remains essential within a One Health framework to limit the spread of resistant bacteria and resistance genes to cohabiting humans.
]]>Authors: Cláudia M. Ferreira Ana C. Silvestre-Ferreira
Trap–neuter–return (TNR) programs are the leading non-lethal approach to managing free-roaming cat populations. When cats are anesthetized for surgical sterilization, veterinarians have a brief opportunity to obtain blood samples for pre-surgical screening, infectious-disease surveillance, and contribution to population-level reference-interval research. Field conditions, however, make collecting good-quality blood samples difficult: cats are often dehydrated and stressed, the procedure is kept brief, and their medical history is unknown. This narrative review draws on the American Society for Veterinary Clinical Pathology (ASVCP) reference-interval guidelines, feline anesthesia and the blood-collection literature, and operational experience from Portuguese TNR programs to describe seven points in the sampling workflow at which technique most often fails: anesthetic depth verification, controlled aspiration speed, immediate EDTA-tube mixing, post-venipuncture hemostasis, hemolysis recognition, precise FIV/FeLV point-of-care test timing, and adherence to a maximum-attempts limit. Three recurrent failure modes—collapsed veins from excessive aspiration, clotted EDTA samples from inadequate mixing, and hematoma formation from insufficient hemostasis—account for the majority of poor samples observed in routine practice. The framework presented here is intended to support reproducible, welfare-compliant sample collection during TNR operations, giving veterinary teams a single, structured reference that supports both animal welfare and the diagnostic value of the samples they collect.
]]>Authors: Vinícius Proença da Silveira Diéssy Kipper Vagner Reinaldo Zingali Bueno Pereira Rafael Sartori Flores Weslei de Oliveira Santana Laura Pancich Diesel Raquel Redaelli Nilo Ikuta André Salvador Kazantzi Fonseca André Felipe Streck Vagner Ricardo Lunge
Feline panleukopenia is a viral disease of domestic cats caused by Protoparvovirus carnivoran 1. This virus species is generally classified into feline parvovirus (FPV) and canine parvovirus type 2 (CPV-2) according to phylogenetic characteristics and association with diseases in the animal hosts. Despite the availability of vaccines, feline panleukopenia has been reported in cats from Brazil. This study aimed to detect and characterize Protoparvovirus carnivoran 1 in blood samples from 368 domestic cats in southern Brazil. Total DNA was extracted from these samples, and FPV/CPV-2 detection was performed using a specific real-time polymerase chain reaction (qPCR) assay. The viral protein 2 (VP2) gene was further sequenced to identify the FPV/CPV-2 genotype. The results showed nine blood samples (2.45%) testing positive for Protoparvovirus carnivoran 1. Partial VP2 gene was successfully sequenced in six of these positive samples. A phylogenetic analysis with a large dataset classified all protoparvoviruses into three main clades: A, B, and CPV. Four of the viruses sequenced in this study (IDs 9917, 9919, 9927, and 9936) were classified in clade A, one (ID 9944) in clade B, and one (ID 9930) in clade CPV. Furthermore, three FPVs from clade A showed total nucleotide identity among themselves and also with field FPV sequences, while two of them (IDs 9936 and 9944) showed high nucleotide identity with two different modified live virus vaccines (from Clades A and B, respectively). These findings suggest that domestic cats are infected by field-like and/or vaccine-related FPV strains, as well as CPV-2 in southern Brazil.
]]>Authors: Maria Soares Carolina Barroso Tiago Aires António J. M. Fonseca Ana R. J. Cabrita
Industrial processing of tomato generates large amounts of tomato pomace (TP), whose disposal and stabilization are challenging due to its high moisture content. This study evaluated, for the first time, the effects of increasing inclusion levels of enzymatic pre-treatment, vacuum paddle-dehydrated TP (ETP), in extruded diets for adult dogs. Three diets containing 0%, 2% or 4% ETP, replacing wheat bran and sunflower meal, were produced. Three two-bowl tests assessed palatability, and a feeding trial was performed using a four 3 × 3 Latin square design with 12 healthy adult Beagle dogs across three 28-day periods. Inclusion of ETP had negligible effects on the chemical composition of diets and kept unaffected palatability, body weight, food intake, fecal consistency and output. Fecal pH was lower, and valerate proportion was higher, in dogs fed the 2% ETP diet. All diets exhibited high digestibility (>90%) without effects of dietary treatments. Overall, ETP, a locally sourced co-product of the food industry, may represent a more sustainable alternative to imported raw materials, although further studies are needed to explore its effects on fecal microbiota and health-related parameters.
]]>Authors: Luca Bellini
Intraoperative anaesthetic monitoring is essential in rabbits due to their high perioperative morbidity and mortality and the limited availability of species-specific evidence, despite their increasing role as companion animals. This narrative review summarises the available scientific literature on intraoperative monitoring in anaesthetised rabbits, focusing on central nervous system assessment, cardiovascular and respiratory monitoring, and temperature management during general anaesthesia. Findings indicate that anaesthetic depth assessment based solely on ocular reflexes is unreliable and should be integrated with jaw tone, reflex responses, and respiratory patterns. Cardiovascular monitoring relies on heart rate, electrocardiography, and arterial pressure measurement, although invasive and non-invasive techniques have limitations in accuracy and practicability in small-size patients. Pulse oximetry and capnography assess oxygenation and ventilation but may be affected by peripheral perfusion, equipment dead space, and technical limitations. Temperature monitoring is critical due to high risk of hypothermia, with continuous or frequent measurements recommended. Overall, multimodal monitoring improves detection of physiological disturbances and supports safer anaesthetic management.
]]>Authors: Vassiliki Tsioli Mandalena Markou Konstantinos Tsafas Mariana S. Barbagianni Epameinondas Loukopoulos Eugenia Flouraki
Phimosis is rarely reported in cats and involves the inability to exteriorize the penis through an absent or abnormally narrowed preputial orifice. This case report describes the clinical presentation, surgical management, and outcome of two male domestic shorthair kittens with suspected congenital type II phimosis. Both cats presented with recurrent stranguria and dysuria, preputial swelling, urine pooling or dribbling, a markedly stenotic preputial orifice, and penile–preputial adhesions. In Case 1, cystic struvite urolithiasis was also identified; treatment included cystotomy and ventrally performed modified preputial urethrostomy because of extensive adhesions, penile hypoplasia, and limited penile mobility. In Case 2, adhesions were limited to the distal penis and were released, allowing circumferential preputioplasty. No postoperative complications occurred. Clinical signs resolved, and normal urination was recorded during available follow-up, which was 12 months in Case 1 and 2 months in Case 2. The congenital origin was suspected based on early onset of clinical signs but could not be definitively confirmed. These cases suggest that selected feline type II phimosis cases can be managed successfully by tailoring surgery to anatomical abnormalities, using ventral-modified preputial urethrostomy when adhesions and penile hypoplasia restrict mobilization, or circumferential preputioplasty when adhesions are limited distally.
]]>Authors: Annabel Bakker Roos Duvekot Michelle Hermans Erik Noorman Stefanie Veraa Paul J. J. Mandigers
Background: Chiari-like malformation (CM) and syringomyelia (SM) are common neurological disorders in Pomeranian dogs. The familial aggregation of these disorders has not been thoroughly characterised in this breed. Methods: A cohort of 999 Pomeranians examined with MRI for CM/SM was retrospectively reviewed. Siblings within litters were identified and compared, and the familial aggregation between offspring and parents screened for these conditions was investigated. Results: The mean central canal dilation (CCD) increased statistically significantly with the age of the dog at the time of the MRI scan. Forty-three per cent of all the dogs were free of CM, with 57% had CM. SM was detected in 31% of all the dogs, while 69% were not affected. Only 37% of all the dogs were free of both conditions. SM was observed in 6% of all the dogs without being affected by CM. Thirty-nine litters were identified in which two or more siblings were scanned. Siblings within the same litter had comparable scans in almost all the cases. Although pups from affected parents have a higher risk of SM, and pups bred from unaffected parents have a lower incidence of SM, the statistical significance could only be demonstrated for the >3 years of age category of SM in offspring, compared with the presence of SM in the mother. It could not be demonstrated for CM. Conclusions: Both CM and SM are frequently observed in this breed, and SM tends to progress over time. Siblings often show comparable findings. The screening results obtained from mothers aged >3 years indicated that SM-affected mothers are more likely to have affected offspring. CM/SM are most likely heritable traits in this breed, and to reduce the frequency of affected pups, breeders are encouraged to screen their breeding stock and use only unaffected parents.
]]>Authors: Jia Xu Wenxin Dai Camila Tagliani Haobo Li Chaoyu Wen Dongyou Yu
Background: Nutrition is a key determinant of skin barrier function and coat quality in cats, but controlled studies comparing commercial diets are limited. Methods: Eighteen healthy adult cats (3.44 ± 0.69 years) were randomly assigned to receive either a standard commercial chicken-based diet (ORI, Instinct Petfood, Visalia, CA, USA, n = 9) or a nutrient-enriched formula designed for skin and coat health (ULT, Instinct Petfood, Little Falls, MN, USA, n = 9) for 12 weeks. Body weight (BW) and body condition score (BCS) were recorded weekly. Visual coat scoring, quantitative descriptive analysis (QDA), and skin biophysical parameters (hydration, sebum, transepidermal water loss) were assessed on days 0, 21, 42, 63 and 84. Results: Both diets maintained stable BW and BCS. Visual scoring showed skin and hair remained within the normal healthy ranges in both groups throughout the study. QDA revealed significant improvements in coat glossiness, softness, and overall scores over time in both diet groups (p < 0.05). Back hydration increased over time, and in the ORI group, values at days 42, 63, and 84 were higher than baseline (44.96, 43.10, and 41.26 vs. 33.85; p < 0.05). Ear hydration showed a significant time × group interaction (p < 0.05). In the ULT group, ear hydration was higher at days 21, 42, 63, and 84 than at baseline (24.17, 35.21, 28.69, and 34.19 vs. 17.87; p < 0.05). Ear TEWL also showed a significant time × group interaction (p < 0.05) and displayed a biphasic pattern in the ULT group, with values at days 63 and 84 higher than at day 42 (15.51 and 14.48 vs. 11.77 g/m2/h; p < 0.05). Compared with the ORI group, the ULT group showed a smaller change in ear TEWL from baseline at day 42 (−2.54 vs. 2.98 g/m2/h; p < 0.05) and a greater increase in ear hydration from baseline at day 84 (16.32 vs. 9.31; p < 0.05). Conclusions: These findings indicate that both diets supported healthy skin and coat conditions in cats, while the nutrient-enriched formula may provide additional benefits for skin hydration.
]]>Authors: Theodora Zacharopoulou Pagona G. Gouletsou Vassilis Skampardonis Stefanos G. Georgiou Dimitrios Doukas Apostolos D. Galatos Eugenia Flouraki Eleftheria Dermisiadou Chryssoula Margeti Vassiliki Tsioli
Skin wounds in dogs and cats are frequently managed by second-intention healing, yet comparative evidence on topical treatments in these species remains limited. This study investigated wound-healing dynamics in dogs and cats using a standardized experimental model that enabled direct interspecies and within-animal comparison of treatment responses during secondary-intention healing. Standardized full-thickness cutaneous wounds were created on the dorsolateral trunk, with multiple wounds per animal randomly assigned to different treatments, including Manuka honey, Dermapliq, and untreated controls. Healing was evaluated using a multimodal approach, including clinical endpoints, planimetric analysis of epithelialization, contraction and total healing, ultrasonographic assessment, and blinded histopathology. Planimetry-derived total wound healing, the primary endpoint, did not differ significantly among treatments, while treatment-associated differences were identified in selected secondary endpoints. Dermapliq-treated wounds reached complete epithelial coverage earlier and showed smaller ultrasonographic wound areas than untreated controls, whereas Manuka honey was associated with faster complete granulation coverage but greater exudate quantity. Interspecies differences were also identified, with cats generally exhibiting slower progression to key healing endpoints than dogs under identical experimental conditions. These findings suggest species-specific healing responses and phase-dependent treatment-associated differences during secondary-intention wound healing in a controlled experimental model. However, they should not be interpreted as evidence of broad treatment superiority. Further studies in naturally occurring, larger, contaminated, infected, or chronic wounds are required before direct clinical extrapolation.
]]>Authors: Lori Kogan Jennifer Currin-McCulloch Valerie Bogie
Dog behavior problems are a common source of stress for owners, yet the psychological mechanisms underlying this distress remain poorly understood. This cross-sectional study online survey examines relationships among dog behavioral characteristics, caregiver guilt, burden, psychological outcomes, social context, veterinary communication quality, and decision-making regarding rehoming and euthanasia. Among 565 participants, 33% met criteria for probable anxiety, 20% for probable depression, and 78% for elevated caregiver burden; 53% reported feeling alone, and 40% reported feeling socially isolated. Dog behavior burden was associated with caregiver guilt, caregiver burden, and decision-making related to euthanasia and rehoming, but not with depression or life satisfaction. In contrast, caregiver guilt was consistently associated with higher anxiety, higher depression, greater caregiver burden, and lower life satisfaction. Informal and self-guided resources were widely used but perceived as less effective, and although veterinarians were commonly consulted, they were rated as less helpful than board-certified veterinary behaviorists, trainers, or prescribed medications. Findings suggest that caregiver distress may be driven less by the objective severity of behavior problems and more by how these experiences are interpreted, evaluated, and socially reinforced, highlighting the importance of addressing caregiver experiences alongside canine behavior.
]]>Authors: Valentina Gazzano Cristina Curreli Maria Claudia Curadi Francesca Cecchi Paolo Baragli Angelo Gazzano Asahi Ogi
French Bulldogs are one of the most popular companion dog breeds worldwide, yet breed-specific behavioral data remain limited. This study aimed to characterize the behavioral profile of French Bulldogs in Italy using the Canine Behavioral Assessment and Research Questionnaire (C-BARQ) and to explore the relationships among sex, neuter status, coat color, and behavioral traits. An online survey was completed by 206 owners of French Bulldogs aged one year or older residing in Italy. Behavioral items were analyzed using ordinal logistic regression with sex, neuter status, and coat color as simultaneous predictors. French Bulldogs showed low aggression and fear-related behaviors, high attachment and attention-seeking tendencies, and moderate excitability and trainability. Sex was the most consistently associated predictor (17 items), with females showing lower odds for sexually dimorphic, fear-related, excitability, and separation-related behaviors. Neuter status showed limited, behavior-specific associations (4 items) involving leash-pulling, separation-related behaviors, and play-related excitability. Coat color was associated with ten behavioral items, including coprophagia, food stealing, hyperactivity, and submissive urination. Most pseudo-R2 values were low (range: 0.009–0.059), reflecting the multifactorial nature of canine behavior. These findings offer a preliminary behavioral characterization of French Bulldogs in Italy and suggest that sex, neuter status, and coat color represent relevant sources of within-breed variation, warranting further research.
]]>Authors: Marcio C. Costa Sara Arghavani Marine Rullier Laura Franco-Garcia Daniela Fischer
Periodontal disease is one of the most common oral conditions in dogs. The oral microbiome plays a key role in maintaining oral health, yet the normal canine oral microbiota and the effects of dental cleaning remain understudied. This study investigated changes in the oral microbiota of healthy dogs after routine tartar removal. Fourteen healthy adult Beagles underwent dental cleaning under general anesthesia. Oral microbiota was sampled using swabs at D-03 (baseline), D0 (after tartar removal), D3, D7, D14, and D28. Microbiota composition was analyzed using 16S rRNA gene sequencing (V4 region). Alpha diversity analysis showed that tartar removal was associated with a significant decrease in richness (Kruskal–Wallis test, p < 0.001) but not with diversity (Shannon). Beta diversity analysis revealed significant differences (PERMANOVA; p < 0.05) across all sampling times compared with baseline. Dental tartar removal temporarily reduced several anaerobic taxa and increased aerotolerant bacteria, with partial recovery toward baseline within two weeks, indicating resilience of the oral microbiota. Porphyromonas dominated the oral microbiota but decreased following dental cleaning with concomitant increases in other bacterial species, notably Neisseria, Moraxella, and Pasteurella. These findings suggest that the canine oral microbiota demonstrates considerable resilience following mechanical disruption by dental cleaning. Future studies should focus on the importance of this microbial restructuring in the pathogenesis and clinical management of canine periodontitis and may inform the development of microbiota-targeted preventive or therapeutic strategies in veterinary dentistry.
]]>Authors: Aylesse Sordillo Jonna Heldrich Raphaël Turcotte Ravi U. Sheth
Postbiotics are an emerging ingredient class which have promising potential to support canine gut function by delivering beneficial microbial compounds directly to the gut. We tested a canine immune health postbiotic (CIHP) in a randomized, double-blind, placebo-controlled study of twenty colony-housed dogs (ten per group) selected for having consistently loose stools but with no diagnosed gastrointestinal disease. After a 5-day wash-in and 5-day baseline, dogs received 12 mg/kg body weight per day of CIHP or a placebo for 28 days mixed with their normal dry diet. The primary outcome was stool consistency (Waltham fecal score), measured on Days 0, 14, and 28; secondary outcomes included fecal gut-health biomarkers and fecal microbiome composition from 16S rRNA sequencing, measured on Days 0 and 28. CIHP improved stool quality (p-value = 0.03), while placebo did not (p-value = 0.5), and CIHP showed a trend toward increasing the odds of individual dogs showing improved fecal scores by Day 28 compared to placebo (p-value = 0.07). Microbiome profiling revealed broader community remodeling with CIHP than the placebo (16 taxa significantly shifted with CIHP vs. 1 with the placebo), consistent with stool quality being impacted by both gastrointestinal and gut microbiome functions. Fecal biomarkers that reflect gut health (pH, dry matter, short-chain fatty acids, dysbiosis index, calprotectin) were within reference ranges at baseline and remained stable in both groups, indicating benefits occurred within a normal physiological window. Together, these findings show that CIHP can improve stool consistency while reshaping the gut microbiome in otherwise healthy dogs prone to loose stools. Future studies in home-environment dogs across breeds, ages, and living conditions are needed to generalize these findings to the broader canine population.
]]>Authors: Anna Misztal-Kunecka Stanisław Dzimira
Tooth structure anomalies, though not frequently described, can be observed in the external appearance not only of humans but also of dogs. The emergence of an oversized set of teeth is not solely an esthetic and health problem, but often also leads to behavioral issues. In the commonly available medical literature, there are many cases describing the occurrence and treatment of double teeth, both in the incisors and in the cheek teeth. In this article, the authors describe a clinical case of a one-year-old female Golden Retriever dog diagnosed with a double tooth in her permanent dentition. The complex morphology of the affected tooth posed a considerable diagnostic and therapeutic challenge. Surgical separation of the tooth was performed, requiring an individualized treatment approach and the adaptation of protocols derived primarily from human dentistry. This case highlights the diagnostic and clinical decision-making difficulties associated with such anomalies. It also emphasizes the importance of tailored therapeutic strategies for managing rare dental developmental abnormalities in dogs. The teeth were successfully surgically separated, and thus the present report contributes to the limited body of literature on double teeth in veterinary patients and provides practical insight into their management. This description of the first case of this type in veterinary dentistry aims to encourage readers to explore the topic of double teeth and demonstrates a non-standard way of thinking and dealing with such a tooth. The presented case is unique from both a diagnostic and therapeutic perspective. Previous publications have focused on these abnormalities as separate entities, failing to consider their simultaneous occurrence within a single tooth. The therapeutic approach employed also deserves particular attention. The most commonly recommended approach involves extraction of the affected teeth; however, in this case, an attempt was made to separate and preserve them, representing a novel clinical approach.
]]>Authors: Michelle Smit Ina Draganova Christopher J. Andrews Rene A. Corner-Thomas David G. Thomas
Cats are among the most popular pets globa lly, yet little is known about how the home environment influences their behaviour. Most studies have focused on cats in shelters or research facilities, potentially limiting applicability to pet cats. This study combined behavioural data from cats in three housing conditions: indoor pet (n = 10), free-roaming pet (n = 18), and research (n = 8), collected in summer and winter. Eight behaviours were classified from collar-mounted accelerometer data using a validated machine learning model and analysed using generalised linear mixed models. Free-roaming pet cats were more active in summer than winter (3.9 ± 0.39% vs. 2.7 ± 0.33%; p < 0.001) and more active than both research (2.0 ± 0.36%; p = 0.004) and indoor pet cats (2.0 ± 0.36%; p < 0.001) in summer. Research cats spent more time lying (52.9 ± 2.03% vs. 36.9 ± 2.89%; p = 0.009) and eating (7.8 ± 0.41% vs. 2.4 ± 0.39%; p = 0.003) in winter than summer, whereas no seasonal differences in these behaviours were observed for pet cats. A bimodal daily activity pattern, with peaks around sunrise and sunset, was observed across housing conditions and seasons. These findings demonstrate that both housing and seasonal conditions influence domestic cat behaviour and should be considered when interpreting behavioural studies.
]]>Authors: Ariel Cañón-Pérez Álvaro Berenguel-Fernandez Iris Giménez-Muñoz Natalia Aguilar-Gallego Maria de los Reyes Marti-Scharfhausen-Sanchez Javier Engel-Manchado
A 2-year-old female Labrador Retriever, with a body condition score of 6/9, underwent ovariohysterectomy 24 h prior at another center and was urgently referred for a possible exploratory laparotomy. The dog presented with lethargy, abdominal pain, and a haematoma with active bleeding in the surgical wound, indicating a possible haemoabdomen. An abdominal-Focused Assessment with Sonography for Trauma (A-FAST) revealed fluid in all four quadrants (abdominal fluid score-AFS 4/4) without abdominal distension, corresponding to an effusion with a packed cell volume of 15% and 4 g/dL of protein. Haematological analysis showed a slight decrease in haematocrit (HCT) and red blood cells, with neutrophilia, while the rest of the blood tests were within normal limits. Physical examination parameters were mostly normal, except for cardiac auscultation where tachycardia, irregular rhythm, and pulse deficit were noted, with normal blood pressure. The electrocardiogram (ECG) indicated both monomorphic and polymorphic ventricular tachycardia with isolated episodes of sinus tachycardia. Treatment included the administration of metamizole, methadone, and maintenance fluid therapy, along with compressive abdominal bandaging. Lidocaine and continuous infusion of fentanyl therapy were initiated. The dog’s HCT, platelets, temperature, and blood pressure remained in the normal range. During the first 8 h, both the ECG and A-FAST showed no relevant changes. From the 9th hour onward, there was a predominance of sinus rhythm, the free fluid decreased to AFS 1/4, allowing for the gradual suspension of lidocaine. Tests for Leishmania, Ehrlichia, Anaplasma, Babesia, and Dirofilaria were negative. The evolution remained favorable, and the dog was discharged after 72 h, showing a good outcome in the cardiology follow-up 5 days later.
]]>Authors: María Daniela Frade-Pérez Rosa Martha Pérez-Serrano Sergio Francisco Cornelio-Martínez María Laura González-Dávalos Yezenia Rubio-Venegas Enrique Flores-Gasca Alfredo Varela-Echavarría María Ofelia Mora-Izaguirre
Dogs, especially as pets but also an increasing number of stray dogs, share environments with humans, facilitating the transfer of antibiotic resistance genes (ARGs) between genetic compartments, with zoonotic and public health implications that must be addressed within One Health. In this cross-sectional comparative study, we explored the distribution of seven selected clinically relevant ARGs in both genomic DNA (gDNA) and plasmid DNA (pDNA), and the phenotypic resistance profile of the cultivable microbiota, between pet dogs (PeDs, n = 12) and free-roaming dogs (FRDs, n = 10) in Mexico. Tetracycline resistance genes (tetQ, tetW, and tetM) predominated in both compartments (40% to 100%), suggesting the presence of a core tetracycline-associated resistome. In contrast, plasmid-associated differences were group-specific: in pDNA cfxA was enriched in FRDs (90%) and tetK in PeDs (42%), whereas blaTEM-1 and ermC were absent in two dog populations. Cultivable bacteria from both groups exhibited phenotypic multidrug resistance, particularly by β-lactams, macrolides, lincosamides, and tetracyclines. FRDs also harbored pathogenic–zoonotic bacteria such as Yersinia enterocolitica, Campylobacter jejuni, and Enterococcus faecalis. Our findings indicated that FRDs and PeDs harbor substantial resistomes, with differences in plasmid-associated ARGs, revealing a transfer potential related to environmental exposure.
]]>Authors: Emma C. Brown Jen Currin-McCulloch Sohaila Jafarian Lori Kogan
Animal-assisted services (AAS) involve providers working in partnership with specially trained animals to deliver therapeutic, educational, and supportive benefits that promote human well-being. Although research shows dog-assisted AAS benefits recipients, little is known about handlers’ motivations and how this work impacts handlers and their dogs. This study explores why individuals engage in AAS with their dogs and the relational benefits and challenges involved. A convenience sample of adult AAS handlers was recruited through various organizations via newsletters and social media. Participants (N = 247), predominantly older, white, and highly educated women, responded to three open-ended survey questions. Data were analyzed using reflexive thematic analysis, which revealed several interconnected themes. Handlers described profound joy, pride, and purpose derived from sharing their dogs with others, and an enhanced capacity to support recipients. Participants reported using AAS to augment professional roles, to pay forward acts of kindness, and to enrich their dogs through social interaction. While overwhelmingly meaningful, this work also involves challenges, underscoring the need for organizational support for AAS teams. Training programs should equip handlers to recognize signs of animal stress, navigate demanding contexts, and engage in proactive conversations about rest, retirement, and loss. Normalizing these experiences may promote handler well-being and sustain ethical, relationship-centered AAS practice.
]]>Authors: Marie-France Humblet Gaëtan Doppagne Claire Deleu Claude Saegerman
The keeping of non-traditional pets has markedly increased in recent years. These species are frequently maintained under inadequate or illegal conditions, raising substantial concerns regarding animal welfare. Authorities are regularly required to confiscate reptiles, arachnids, and other exotic taxa—often in poor health—and to accommodate them temporarily in transit facilities while long-term placement options are identified. Existing facilities are operating beyond capacity, creating an urgent need for the development of additional infrastructure. Given the potential risks associated with these animals, stringent safety measures are essential. Following an assessment of the hazards linked to housing primarily reptiles and arachnids—including risks related to venomous species, transmission of zoonotic pathogens, and the escape of potentially invasive organisms—the present paper provides a set of recommendations for the design and operation of transit housing facilities. The guidance focuses on infrastructure requirements, routine and emergency procedures, and daily management practices for both animals and facilities. Although these recommendations were developed within the specific regulatory framework of Southern Belgium, they may be applied more broadly, provided that local regulations are appropriately considered.
]]>Authors: Tereza Jaresova Natali Bauer Katarina Hazuchova
This study aimed to obtain information on veterinarians’ experience regarding the management of diabetes mellitus (DM) in the United Kingdom and Europe. A questionnaire consisting of two parts (part 1—DM management [28 questions]; part 2—demographic information [9 questions]) was made available online in three languages (English, German, and Czech) to be completed by European veterinarians from May 2022 to February 2024. In total, 488 veterinarians completed the questionnaire. Most respondents worked in first-opinion practice (396/488 [81.1%]) and diagnosed DM in 2–4 dogs (206/488 [42.2%]) and 2–4 cats (250/488 [51.2%]) per year. In both dogs and cats, most respondents recommended twice daily insulin injections (398/488 [81.6%] and 440/448 [90.2%], respectively). Poor owner compliance and the presence of comorbidities were considered the main challenges in DM management by 220/488 (45.1%) and 179/488 (36.7%) veterinarians, respectively. Nevertheless, most owners were willing to start treatment, and only 80/488 (16.4%) respondents for dogs and 114/488 (23.4%) for cats reported that >1 in 10 pets were euthanised after DM was diagnosed. Our results suggest that the management of diabetic pets in Europe fairly corresponds with DM management guidelines, although challenges preventing their full implementation exist.
]]>Authors: Mandalena Markou Thomas Koutis Konstantina Karagianni Ioannis Panopoulos Eugenia Flouraki Vassiliki Tsioli
Congenital external auditory canal atresia (EACA) is a rare developmental anomaly in dogs with limited information to guide management. This report describes the clinical presentation, diagnostic work-up, surgical technique, and long-term outcome in two young dogs with unilateral congenital EACA. An 8-month-old Caucasian Shepherd and a 9-month-old Maltese presented with left-sided otalgia and absence of the external canal opening. Computed tomography identified segmental atresia of the vertical canal with intraluminal soft-tissue attenuating material and mild otitis externa and media in both dogs. Cytology and culture of canal contents were unremarkable, supporting a congenital, non-infectious origin. Both dogs underwent a canal-preserving vertical canal-to-external acoustic meatus anastomosis using a pull-through approach. Minor postoperative complications (partial wound dehiscence, mild canal stenosis) were successfully managed. At the 5-year and 1.5-year follow-up, respectively, both dogs remained free of clinical signs, with preserved hearing, supporting this procedure as a functional, cosmetically acceptable option in carefully selected dogs.
]]>Authors: Audrey Koh Rui Ping Shamsaldeen Ibrahim Saeed Mohammed D. Goni Nor Fadhilah Kamaruzzaman
Pet insurance is a financial tool meant to provide financial support to pet owners for veterinary expenses. It was introduced to the Malaysian market in 2010 with several companies offering various subscription packages. However, public awareness of pet insurance has been low compared to other pet-related industries in Malaysia for the past five years. There is also a lack of research on the acceptance and adoption of pet insurance by pet owners in Malaysia. Therefore, this study aims to (i) assess the knowledge, attitudes, and practices (KAPs) of pet owners regarding pet insurance in Selangor, Malaysia, (ii) determine the associations of sociodemographic factors with the KAP levels, and (iii) determine the correlation between the levels of KAPs. A cross-sectional survey was conducted in Selangor, Malaysia, and 116 pet owners participated. Data were subjected to descriptive analysis statistics, followed by Chi-square tests of association and a Spearman correlation analysis, which were all performed using IBM SPSS Statistic version 27.0. The results showed that most of the respondents have poor knowledge of (n = 63, 54.3%), moderate attitudes towards (n = 63, 54.3%), and poor practices regarding (n = 87, 75.0%) pet insurance. Significant associations were indicated between income range per month and knowledge and practice (p < 0.05), highest education level and attitude (p < 0.05), and locality of residence and practice (p < 0.05). There is a significant correlation between total knowledge and practice scores (r = 0.649). The poor knowledge of insurance among pet owners in Selangor indicated the need to further increase the awareness of pet owners regarding the benefits of pet insurance through enhanced marketing and public education initiatives.
]]>Authors: Zoé Goullet Marietta Máté László Ózsvári
Pet health insurance can reduce the financial burden of veterinary care and ensure adequate treatment for companion animals. In France, where 67% of households own at least one pet and 68% of owners consider them family members, awareness of pet insurance reaches 94%, yet only around 5–6% of pets are insured. This review aims to provide an overview of the French pet health insurance market, analysing its structure, coverage options, and potential implications for veterinary practice. A literature review was conducted using French and English sources, complemented by simulated price quotes from major insurance companies for four virtual pets (two dogs and two cats). The analysis compared 11 major French pet insurance providers across criteria such as species covered, waiting periods, age limits, coverage rates, reimbursement mechanisms, and preventive care benefits. The results reveal significant variability in coverage options, preventive care allowances, and reimbursement procedures. Across providers, simulated annual premiums for the virtual pets ranged from EUR 71.76 to EUR 1426.44, with reimbursement rates of 50–100% and annual caps of EUR 763–2500. It can be concluded that pet insurance may help owners manage unexpected veterinary costs and encourage preventive care. However, subscription rates remain low due to limited understanding of insurance plans and perceived high costs. Wider adoption of pet insurance could improve access to care and ensure fair remuneration for veterinarians.
]]>Authors: Meike Rombach David L Dean
The present study is dedicated to exploring key factors impacting US pet owners’ preferences for brand, price, country of origin, and health and nutrition claims as important extrinsic and credence attributes. Pet engagement and subjective and objective knowledge, as well as varying forms of pet humanisation behaviour, were thought to be suitable factors. The study is of an explorative and quantitative nature, rooted in an online consumer survey, descriptive statistics, and partial least squares structural equation modelling (PLS-SEM). To strengthen the PLS-SEM model, relative preference shares derived from a best–worst analysis were integrated into the model. The results with the strongest effect sizes indicate that US pet owners’ objective knowledge is positively associated with pet non-humanisation behaviour, those who actively engage with their pet are positively associated with loving humanisation behaviour, and that health and nutritional claims on pet food are less important for those reporting non-humanisation behaviours. The analysis between the varying types of pet humanisation behaviours and the best–worst-derived relative preferences for extrinsic and credence attributes provides a diverse picture. Together, the results suggest that pet engagement and both subjective and objective knowledge are associated with pet humanisation behaviour, which are differentially linked to the importance of pet food product attributes. Best practice recommendations for marketers in the pet food industry are provided.
]]>Authors: Andressa Rodrigues Amaral Pedro Henrique Marchi Natália Manuela Cardoso de Oliveira Beatriz Leme do Carmo Larissa Wünsche Risolia Carlos Eduardo Larsson Júlio Cesar de Carvalho Balieiro Thiago Henrique Annibale Vendramini
Beta-glucans are immunomodulatory compounds known to act as adjuvants in the beneficial regulation of various allergic conditions, particularly atopic dermatitis, as well as in the modulation of dysbiosis in patients with chronic gastrointestinal disorders. However, certain underlying conditions, such as inflammatory bowel disease (IBD) and the associated loss of oral tolerance, may alter the expected outcomes of supplementation and lead to dysregulated immune responses. This study reports cases of cutaneous allergic reactions in dogs with IBD following the oral administration of yeast-derived beta-glucan. Two dogs with controlled IBD received 9.13 ± 1.14 mg/kg of beta-glucan in a double-blind study designed to investigate its effects on the intestinal microbiota in IBD. Both animals were withdrawn from the study due to adverse effects, including cutaneous allergic manifestations and intense pruritus. These findings suggest that patients with impaired oral tolerance may develop cutaneous reactions following the ingestion of yeast-derived prebiotics.
]]>Authors: Huijun Lee Omid Nekouei Yat Ming Tom Chung Melissa Upjohn Julia Beatty
Brachycephalic dogs commonly suffer from breathing difficulties and other chronic health problems, yet strong consumer demand is widely reported. Whether the popularity of brachycephalic breeds persists across different contexts is largely untested. Census data from 1,510,085 canine consultations conducted at a single veterinary hospital from 2004 to 2023 in Hong Kong were collected and analysed. Individual dogs (n = 96,777) were counted once, in the first year of presentation. Males (53.5% to 57.7%) and purebreds (86.2% to 91.2%) predominated each year. Neutered dogs increased from 52% to 70% annually. Poodles were the most popular breed from 2009. Brachycephalic breeds, collectively, declined progressively from 36.3% to 15.4% of the annual population. This trend was exaggerated in dogs < 1-year-old (27.4% to 11.7%). Absolute numbers of common brachycephalic breeds declined, except for French Bulldogs and Pugs. A historical cohort study was conducted with a subset of the data (2016–2023) to investigate the hypothesis that veterinary service use would be higher in brachycephalic breeds compared with other groups. The hypothesis was proven. Relative demand for brachycephalic breeds in Hong Kong is decreasing, with the exception of French Bulldogs and Pugs. Investigating drivers of these observations will inform behaviour change strategies, and facilitate comparisons across different regions.
]]>Authors: Filomena Meduri Claudia Rifici Pietro Gambadauro Diego Antonio Sicuso Gianluca Novari Giuseppe Mazzullo Michela Pugliese
Viper envenomation in dogs represents a significant medical emergency in regions where vipers are endemic. Despite its clinical relevance, detailed data on the haematological and biochemical alterations in canine viper envenomation remain limited. This study aimed to evaluate the clinical presentation and haematological, biochemical and coagulative changes occurring in dogs following bites from the Vipera aspis species, and to assess their diagnostic and prognostic significance. Twelve dogs with suspected Vipera aspis envenomation were encompassed in the study. Clinical data were gathered and blood samples were collected at hospital admission (T1), 24 h (T2) and 48 h later (T3). Complete blood counts, biochemical profiles and coagulation parameters were analysed using standard automated systems. Common clinical signs included local pain and swelling, depression, fever, haematuria and melena. Haematological evaluation revealed progressive anaemia, leucocytosis and thrombocytopenia. Biochemical findings showed elevated alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and creatine kinas (CK), indicating hepatic and muscular injury; however, no consistent evidence of renal failure was found. Coagulation analysis revealed a significant shortening of activated partial thromboplastin time (aPTT) and prothrombin time (PT) over time, alongside marked increases in fibrinogen and antithrombin III. This indicates an inflammatory rather than consumptive coagulopathy. Viper envenomation in dogs induces complex haematological and biochemical alterations, reflecting both direct venom toxicity and systemic inflammatory responses. Early recognition, supportive care and continuous laboratory monitoring are essential for improving prognosis.
]]>Authors: Alexander Schreiber Chih-Chun Chen Jan S. Suchodolski Betty Chow
Protein-losing enteropathy (PLE) is a spectrum of gastrointestinal disorders in which protein loss occurs through the gastrointestinal tract. One of the underlying causes is chronic inflammatory enteropathy (CIE). Conventional therapies for CIE often include diet, immunosuppressives, anti-microbials, probiotics, and, recently, fecal microbial transplantation (FMT). This case report highlights the use of lyophilized material-based FMT through oral capsules and enema in a dog with PLE and concurrent protein-losing nephropathy (PLN). The patient initially had a significantly increased dysbiosis index (DI) and required repeated FMT treatments, resulting in a positive clinical response through improvement in body weight, serum albumin concentrations, fecal scores, and normalization of the DI over time. To maintain clinical responses, FMT had to be performed monthly. Approximately 1 year after starting FMT therapy, the patient then developed an episode of acute hemorrhagic diarrhea syndrome (AHDS) associated with netF-gene-encoding Clostridium perfringens strains, after which the DI became abnormal again. The patient responded clinically well to monthly FMT treatments again, but it took several months for normalization of the DI after the AHDS episode. In summary, this case report highlights the continued use of adjunct lyophilized FMT in a dog with PLE resulting in improved clinical control over time.
]]>Authors: Anna-Chiara Riedl Katharina Charlotte Jensen Katja Schirl Ines Hoffmann Joshua Schwinn Robert Klopfleisch Heike Aupperle-Lellbach
This retrospective study provides an extensive evaluation of feline squamous cell carcinoma (SCC), comparing oral and cutaneous SCC, as well as different oral/cutaneous sites, with respect to sex, age, breed, and coat length. It was based on 4300 SCCs submitted to LABOKLIN GmbH & Co. KG from 2017 to 2023. No sex predisposition was identified. Affected cats were predominantly older (median age 13 years). SCC risk increased with age, although cats of very young age were also affected. Breed predispositions were not found. However, compared to non-pedigree cats, Persians, Norwegian Forest Cats, British Shorthairs (BSH), Chartreux, and Siamese cats showed a reduced risk, as did longhaired cats. The predominant sites were the oral cavity (41.0%, 1762/4300) and skin (35.8%, 1540/4300). Maine Coons, BSHs, Persians, and Norwegian Forest Cats, as well as longhaired cats, developed oral SCC more frequently. Intraoral, gingival (36.2%, 637/1762) and lingual (19.0%, 334/1762) SCC predominated. Common cutaneous sites included the pinnae (35.0%, 539/1540), unspecified head/neck regions (8.8%, 135/1540), and the nose (8.7%, 134/1540). Maine Coons and BSHs showed less auricular SCC; Sphynx had more on the trunk. These findings emphasise SCC as a crucial differential diagnosis for oral and cutaneous lesions, even in young cats.
]]>Authors: Erika Friedmann Nancy R. Gee Mona Ramadan Abdelhamed Eltantawy Sarah Cole
Research suggests that older adults might obtain health benefits from pet ownership; however, results are mixed. Pet attachment is suggested as both a mechanism for the relationship and a reason for differences in the association of pet ownership with health outcomes. This systematic review examines evidence for the relationship between pet attachment and health outcomes among older adults. The Open Science Foundation-registered review began with 20,795 candidate articles. We limited our review to the 58 articles that consisted of original research, published in peer-reviewed journals between 1965 and June 2025, written in English, included older adults (age ≥ 50 years) or were limited to only older adults, and examined the relationship between pet attachment and health outcomes. The articles included analyses of psychological (n = 53), social (n = 27), or physical (n = 2) health outcomes. Pet attachment was assessed with 19 tools; most frequently the Lexington Attachment to Pets Scale (n = 21) and the Pet Attachment Questionnaire (n = 8). The studies were not consistently of high quality according to OCEBM criteria. Except for grief, which was consistently related to pet attachment, the findings do not support a clear relationship between pet attachment and health outcomes in older adults. Findings suggest that the relationship between pet attachment and health outcomes may be more pronounced in younger than in older adults.
]]>Authors: Angela Zilinger Mary K. Sramek Tarun Chandra Teresa Schmidt Jessica Bagel Andrew Stayduhar James Fryer Gregory D. Sunvold
Probiotics, prebiotics, and postbiotics are of interest for their potential gastrointestinal and immunological benefits in pet health. This study aimed to assess whether a unique blend of Bacillus subtilis, Bacillus clausii, Bacillus coagulans (Weizmannia coagulans), FOS, GOS, and a postbiotic yeast extract could provide beneficial gut and immunological effects when fed to healthy, adult dogs. Twenty-four healthy adult beagle dogs (mean age 5.17 yrs) were fed the probiotic, prebiotic, and yeast chew (PPYC) or control chew (CC) supplement for 31 days, accompanied by fecal and blood sampling. Following 31 days, PPYC fed dogs had decreased (p < 0.05) fecal calprotectin concentration, a biomarker indicative of reduced intestinal inflammation, compared with dogs receiving the CC. In the PPYC group, blood C-reactive protein levels, an indicator of tissue inflammation, tended (p = 0.11) to be reduced. In addition, dogs receiving the PPYC supplement showed an increase in the IL-17a cytokine (p < 0.05). Despite dogs being in a clinically healthy state, changes in some dysbiosis-related bacterial strains were observed. There was an increase (p < 0.05) in the % of total bacteria of Blautia in the PPYC group by the end of the study, as well as an increase in the percent change from Day 0 of C. hiranosis (p < 0.05). Increased alpha diversity, a measure related to the resilience to environmental change, was observed in the PPYC group (p < 0.05). These results suggest that after consuming a supplement containing probiotics, prebiotics and a postbiotic yeast extract, markers of gut and systemic health were improved in otherwise healthy dogs.
]]>Authors: Carlos Antonio Arcos-Roa Luis Arturo Ortíz-Carbajal Jose Luis Bravo-Ramos María Guadalupe Sánchez-Otero Sokani Sánchez-Montes Aarón Bustos-Baena Carolina Palmeros-Exsome Sashenka Bonilla-Rojas Beatriz Agame-Lagunes
Ensuring blood group compatibility is essential in canine transfusion medicine, and Dog Erythrocyte Antigen (DEA) 1 is the most clinically relevant erythrocyte antigen due to its immunogenicity. However, information on its prevalence in Mexico is scarce. This study assessed the distribution of DEA 1 in client-owned dogs from Veracruz–Boca del Río, southeastern Mexico, and applied antigen frequencies to generate theoretical incompatibility scenarios in untyped populations. A cross-sectional study was conducted using blood samples from 192 clinically healthy dogs. DEA 1 typing was performed using a commercial immunochromatographicassay. Frequencies were compared using chi-square or Fisher’s exact test, and 95% confidence intervals were calculated with the Wilson method. Of all dogs sampled, 60.9% (95% CI: 53.6–67.8) were DEA 1-positive. No statistically significant differences were observed by sex or municipality (p > 0.05). Although frequencies varied among breeds, no significant association between breed and DEA 1 status was detected (p > 0.05). Based on observed antigen proportions, mathematical estimations were calculated to illustrate how DEA 1 distribution alone may contribute to potential donor–recipient mismatching when typing is not performed. These estimations are theoretical values and do not represent clinically documented sensitization or transfusion reactions. This study provides the first report of DEA 1 distribution in southeastern Mexico and establishes baseline information that may support future research on canine transfusion practices and donor selection.
]]>Authors: Shiori Kawai Saki Terashima Natsuki Ebashi Mayu Sakurama Miki Ito Yumiko Nakanowataru Takanori Kooriyama
In recent years, requests for animal-assisted interventions (AAI) from medical institutions and welfare facilities have increased. Dogs are the most commonly used animals in AAI. Dogs that pass the “therapy dog” aptitude test can work in AAI. In previous research, we identified the Canine Behavior Assessment and Research Questionnaire (C-BARQ) factors common among dogs that passed the aptitude test. Using these factors, we developed the TC-BARQ, a screening questionnaire for therapy dogs that included 51 C-BARQ surveys. In this study, we conducted TC-BARQ screenings and compared the characteristics of dogs that passed and failed the aptitude test. We collected TC-BARQ data points from aptitude test examinees of the local AAI Dog Association. Each dog is identified by its breed, sex, neutering status, and whether it lives with another dog at home. For each question, we identified factors that differed between dogs that passed and those that failed. As a result, differences emerged in the presence of family dogs, particularly in behaviors related to aggression toward strangers and other dogs, as well as excitability toward people and situations. Continued surveillance is essential, but this study provides important information on selecting “therapy dogs”.
]]>Authors: Mandalena Markou Eleftheria Dermisiadou Konstantina Karagianni Eugenia Flouraki Vassiliki Tsioli
The objective of the present study is to review the anatomical considerations, surgical techniques, clinical applications, and outcomes of myocutaneous and muscle flaps used in the reconstruction of limb defects in dogs and cats. Limb wounds in small animals often result from trauma, neoplasia, or infection and can involve significant soft tissue loss. Reconstruction of these defects is challenging due to limited local skin availability, particularly in distal regions, and the need to preserve function while preventing complications. Muscle and myocutaneous flaps provide well-vascularized tissue suitable for covering complex wounds, especially those with exposed bone, joints, or tendons. This review synthesizes current literature on commonly used flaps—including latissimus dorsi, cutaneous trunci, trapezius, sartorius, semitendinosus, and flexor carpi ulnaris; focusing on their anatomical basis, vascular supply, arc of rotation, surgical technique, indications, and complication rates. Comparative data between dogs and cats are highlighted, and experimental as well as clinical applications are discussed. Myocutaneous flaps offer durable and reliable coverage with lower infection and necrosis rates compared to skin grafts, particularly in contaminated or poorly vascularized wounds. Common complications include distal flap necrosis, wound dehiscence, seroma, and, occasionally, functional deficits. Muscle and myocutaneous flaps remain essential tools in limb reconstruction. Successful outcomes require careful flap planning, surgical expertise, and vigilant postoperative care. Further prospective studies are needed to optimize flap selection and reduce complication rates in both species.
]]>Authors: Nicole R. Pallotta
In recent years, the socio-legal concept of emotional support animals (ESAs) has come under increasing scrutiny in the U.S. Widespread confusion about the legal status of ESAs versus service animals and concerns about misrepresentation have resulted in a backlash and a cascade of new legislation addressing “fake service animals.” However, this reaction reveals a broader social problem rooted in policy lag: the integration of companion animals into society has not kept pace with the rise of multispecies families. This article examines law, policy, and public discourse about ESAs within two distinct social contexts: (1) the emergence of multispecies families alongside the systemic exclusion of companion animals from many private and public spaces, and (2) the medicalization of relational bonds and everyday life. Although well-intentioned, the legal and social construction of ESAs creates a two-tiered system where caregivers of companion animals must acquire a medical diagnosis in order to obtain equal access to basic necessities like housing. In addition to resting on weak evidence, the ESA framework diverts attention from macro social issues by pathologizing the individual, creating a problematic medicalization of the human-animal bond that is ultimately detrimental to the well-being of companion animals, their caregivers, and people with disabilities. The construct is also troublingly anthropocentric, centering what animals can do for humans without considering the psychological and emotional support needs of the animals themselves. This article concludes that the anthropocentric medical model advanced by the ESA construct should be replaced with a multispecies social model that supports the human-animal bond without unnecessary medicalization. This approach advocates for equitable laws and policies, such as the expansion of pet-inclusive housing, that benefit families that include companion animals and the communities in which they are embedded.
]]>Authors: Taisuke Konno Daisuke Kikuchi Hiroyuki Suzuki Yosuke Nishikawa Shigeki Kisara Hitoshi Nakamura Yuriko Murai
Owner-led self-medication for companion animals is a growing global practice; however, empirical data from Japan remain limited. Framing medication safety within a One Health perspective, this study aimed to characterize Japanese pet owners’ use of over-the-counter (OTC) drugs and identify possibilities for pharmacists to support rational self-medication. A cross-sectional 13-item online survey was administered to 500 owners in Japan between 30 May and 2 June 2025. Data on owner demographics, willingness to consult pharmacists, veterinary visit behavior, and OTC purchasing practices were summarized. Many owners were receptive to pharmacy support; 65% wished to consult a pharmacist, and 6.8% had already done so. Overall, 15.2% reported using OTCs drugs, primarily for treatment or prevention and prioritized perceived effectiveness and safety when selecting products. Some owners managed mild pet illnesses at home, citing perceived mildness and cost as reasons for not visiting a veterinary clinic. There is an unmet demand for accessible expert counseling at the point of purchase. Leveraging community pharmacies linked with pet specialty pharmacies as first-contact hubs could promote appropriate self-medication; doing so would require veterinary-specific training, establishing a formal credential for veterinary pharmacists, and defining pharmacist–veterinarian communication to ensure safe and effective use.
]]>Authors: Aimee Clark Kristie E. Cameron
Fear, anxiety, and stress (FAS) in canine patients is a growing concern for the veterinary industry. FAS affects the health of the patient while also increasing the risk to veterinary staff. Studies show that many veterinarians do not feel confident in managing behavioural issues like FAS, making engaging effectively with clients more difficult. Pre-visit pharmaceuticals (PVPs) can be an important tool in mitigating the effects of FAS; however, conclusive research is lacking on the efficacy of many of the medications and combinations currently used for this purpose. A comprehensive literature search was conducted in multiple databases. The most current research available on medications identified as widely used for treating FAS, and available for use in New Zealand, has been reviewed and discussed. Combinations selected for inclusion were the ‘chill protocol’ of acepromazine, gabapentin, and melatonin. Single agents included are clonidine, dexmedetomidine, gabapentin, and trazodone. Research into the use of the ‘chill protocol’ suggests this may be a combination particularly useful in highly stressed and aggressive dogs; however, additional studies need to be conducted to strengthen the evidence for its use. A multimodal approach has been identified as being desirable when addressing FAS in a clinical setting, with less evidence to support the use of single-agent medications in mitigating problematic behaviours.
]]>Authors: Cushla Redmond Ina Draganova Rene Corner-Thomas David Thomas Chris Andrews
In the original publication [...]
]]>Authors: Belén Varela Camila Larrañaga Victoria Yozzi Kanji Yamasaki José Manuel Verdes
We performed an histological study on all cases of canine skin hemangiomas and hemangiosarcomas that were examined between 2018 and 2020. Of the 446 dogs with skin tumors examined, 25 were diagnosed with hemangiomas and 24 were diagnosed with hemangiosarcomas. Mixed-breed dogs were the most commonly affected by both tumors. The average ages of the dogs who were diagnosed with hemangiomas and hemangiosarcomas were 8.70 and 8.36 years, respectively, with the mean age for hemangiosarcomas being slightly higher; however, this difference was not statistically significant. Both types of tumors were more common in the trunk and limbs. Sex differences in tumor frequency were not detected in dogs with hemangiomas or hemangiosarcomas. Histologically, the mixed capillary and cavernous type was the most common in cases of hemangiomas, and the mixed capillary, cavernous, and solid type and the mixed cavernous and solid type were more common in hemangiosarcoma cases compared with the other types. In addition, epithelioid hemangiosarcomas were frequently detected.
]]>Authors: Désirée Tahnee Schmid Karen Barmettler Marc J. A. Stevens Roger Stephan
Raw meat-based diets (RMBDs) have gained popularity over the past few years. However, there is growing concern about their safety. The study objective was to evaluate the prevalence and characteristics of the pathogens Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Campylobacter spp. in frozen commercially sold RMBDs. One hundred raw meat-based diet products (meat originating from Switzerland, Germany, Austria, Spain, Scandinavia, South Africa, and Australia) were purchased online or in local stores and identified using culture-based methods. Isolates were further characterized by phenotypic and whole genome sequencing-based methods. In total, 11% (n = 11) of the samples tested positive for Salmonella spp., 41% (n = 41) for L. monocytogenes, 58% (n = 58) for Y. enterocolitica, and 3% (n = 3) for Campylobacter spp. Other than for Salmonella, there are no legal regulations concerning contamination of RMBDs with these zoonotic pathogens. The results of our study show that it is indicated to reconsider the requirements for the microbiological quality of RMBDs and to further raise awareness regarding the risks of handling and feeding RMBDs.
]]>Authors: Katerina T. Moraiti Ioanna Papavasileiou Evgenia Flouraki Vasiliki Tsioli Shelley J. Newman Joao P. Cavasin Panagiotis G. Xenoulis
A nine-year-old, 10.5 kg, female-spayed Poodle-mix dog presented with a five-day history of vomiting and regurgitation. Thoracic radiographs revealed an area with increased opacity within the esophageal lumen. A ductal esophageal lesion with intraluminal extension and mild left axillary lymphadenopathy were detected on computed tomography. Esophagoscopy revealed a large vascular, obstructive mass with a smooth surface, in the mid part of the esophagus. Endoscopic biopsies were collected, and histopathologic findings were consistent with an inflammatory polyp. Surgical excision of the mass via sternotomy was performed and the mass was sent for histopathologic evaluation. Histopathological examination of the mass revealed an esophageal plasmacytoma with perivascular amyloid deposition, which was confirmed by immunohistochemical staining. There was no evidence of regrowth until 18 months after surgical removal, when evidence of regrowth was identified. The dog had only one episode of vomiting and regurgitation which was resolved after symptomatic treatment.
]]>Authors: Achraf Adib Lesaux Jonas Ghyselinck Cindy Duysburgh Massimo Marzorati Jonna E. B. Koper Jake Burlet
In dogs, gut microbiome dysbiosis is associated with several health conditions, including gastrointestinal disease. Probiotic supplementation can support a balanced gut microbiome. This study assessed the impact of a probiotic containing a mixture of Lacticaseibacillus casei, Limosilactobacillus fermentum, Levilactobacillus brevis, and Enterococcus faecium on the gut microbiota of six dogs using short-term colonic simulations. Two groups were included, i.e., blank versus supplementation with the test product, and incubated for 48 h. Probiotic-supplemented reactors had significantly greater fermentative activity compared with the blank, as shown by lower pH levels and higher gas pressure after 6 h, 24 h, and 48 h of incubation (p < 0.05 for all). Saccharolytic fermentation also increased, with a significantly higher level of acetate at 24 h and propionate at 6 h, 24 h, and 48 h with the test product versus blank (p < 0.05 for all). There was no significant effect of the test product on alpha-diversity, but beta-diversity analysis revealed a clear separation in the microbial community composition between the test product and blank. Eight bacterial taxa were enriched with test product supplementation, including the probiotic test strains as well as Megamonas and Bacteroides species. This study, using in vitro short-term colon simulations with six canine donors, provides insights into the probiotic characteristics of the test product.
]]>Authors: Daiana de Souza Machado Rogério Ribeiro Vicentini Luana da Silva Gonçalves Suzana Luchesi Emma Otta Aline Cristina Sant’Anna
Despite the growing preference for cats as companion animals, beliefs and misperceptions about them are still common. Cultural and sociodemographic aspects can influence society’s attitudes towards cats, affecting the way they are kept and cared for and, consequently, their welfare. This narrative review aimed to identify and categorize beliefs and misconceptions about domestic cats commonly described in the scientific literature, particularly those related to behavior, sociality, health, and management, in order to foster critical thinking about these perceptions. Our bibliographic search included three electronic databases (Web of Science, PubMed and Scopus). This review highlights a set of recurrent beliefs of general society reported by the scientific literature, such as the perception of cats as independent and non-social animals, bringers of bad luck, incapable of forming emotional bonds with their guardians, or in need of outdoor access to thrive. Although widespread, many of these beliefs lack empirical support. Identifying and fostering critical reflection on such beliefs can contribute to deconstructing them, improving the guardian–cat relationship and enhancing the welfare of both cats and humans.
]]>Authors: Heather Dye
Dog parents have a strong attachment to their pets and treat them as children. Similarly to the guilt experienced by the parents of human children, dog parents feel guilty when they have to travel or work long hours and leave their dogs at home alone. This study examined the empathic tendencies among dog parents and how they are related to dog parent guilt and other mental health symptoms. A sample of 332 dog owners from the United States, stratified by age and sex, was recruited for this study. The sample comprised 168 female and 164 male participants. The Guilt About Dog Parenting Scale (GAPS-D), Depression Anxiety and Stress Scale-21, Interpersonal Reactivity Index, and Dog Anthropomorphism Scale were administered via an online survey. Demographic variables, such as age, sex, race, education level, household income, relationship, and parental status, were also collected. Data were analyzed using descriptive statistics, correlation, and linear regression. As predicted, this study found that empathic tendencies in dog parents are related to guilt, anthropomorphizing, and mental health symptoms. This is the first study to examine empathetic tendencies among dog parents in relation to dog parent guilt. Researchers, educators, social workers, mental health professionals, and veterinarians should inform and educate pet owners about dog parent guilt. This will increase the knowledge of professionals, organizations, and pet owners suffering from such guilt. Cognitive behavior therapy (CBT) may offer a promising approach for helping pet parents identify, normalize, and better understand their thoughts, feelings, and behaviors related to empathic and anthropomorphic tendencies. By addressing these cognitive patterns, CBT could potentially help reduce associated feelings of guilt, depression, anxiety, and stress.
]]>Authors: Jocelyn F. Wren Sofia M. Wilson Yifei Kang Patrícia M. Oba John F. Menton Elena Vinay Mathieu Millette Melissa R. Kelly Kelly S. Swanson
Background: Abrupt dietary changes may disrupt gut microbiota populations and lead to gastrointestinal issues. This study aimed to determine the effects of live Bacillus pumilus SG154 or Lacticaseibacillus paracasei 327 postbiotic on fecal characteristics and microbiota populations of dogs following an abrupt diet change. Methods: Twelve healthy adult English pointer dogs (6.38 ± 2.75 yr) were used in a replicated 3 × 3 Latin square design to test the following treatments: (1) placebo (control; 250 mg maltodextrin/d); (2) live B. pumilus [5 × 109 colony-forming units (CFU)/d]; and (3) L. paracasei postbiotic (100 mg; derived from 2 × 109 CFU/d). Each period lasted 42 days, with the diet change occurring on day 28. Fecal samples were scored and analyzed for pH, dry matter content, and microbiota before and 2, 6, 10, and 14 days after the diet change. Results: The abrupt diet change increased (p < 0.01) fecal pH, increased (p < 0.01) the dysbiosis index, decreased (p < 0.0001) fecal dry matter, and led to a large shift in the fecal microbiota community. Fecal scores were lower (p < 0.05) in the B. pumilus group. B. pumilus reduced (p < 0.05) the relative abundance of fecal Prevotella and Muribaculaceae, while both treatments (B. pumilus; L. paracasei) increased (p < 0.05) the relative abundance of fecal Holdemanella. Conclusions: These results suggest that an abrupt diet change leads to large shifts in fecal microbiota and modified fecal characteristics. The supplementation with a B. pumilus probiotic and a L. paracasei postbiotic slightly altered the relative abundance of a few microbial taxa but was unable to attenuate most responses.
]]>Authors: Leena Awawdeh Natalie Waran Rachel H. Forrest
Objective: To investigate public attitudes in New Zealand towards the euthanasia of dogs and cats and to explore the factors that influence these views. Materials and Methods: Data were collected through a nationwide online survey conducted in 2019 as part of the Furry Whānau Wellbeing research project. The survey included questions about pet euthanasia, and respondents were asked to indicate their level of agreement with various statements. Results: Of 2293 respondents to the 2019 New Zealand Pet Survey, the majority (n = 1756) opposed euthanasia for stray, unwanted, or financially burdensome pets (76.7%; 90.2% and 66.3% of these disagreed or strongly disagreed, respectively). In contrast, a slim majority (n = 1162) supported it for sick animals. Females were less likely to agree with euthanasia in most scenarios compared to males. Māori and New Zealand European respondents (n = 1790) showed higher levels of neutrality than other ethnicities regarding stray animals and lower levels of disagreement regarding financially constrained situations. Older respondents (65+) were more likely to support euthanasia for sick animals, while younger respondents (18–24) were less inclined. Higher education levels are correlated with increased neutrality towards euthanising stray animals and increased support for euthanising feral animals. Respondents with children were more likely to be neutral or to disagree with euthanasia in most scenarios. Those with rural upbringings were more accepting of euthanasia for stray and feral animals. A thematic analysis of 653 respondent comments revealed key themes: euthanasia was seen as complex and context-dependent, a humane last resort for suffering animals, but not for convenience. Respondents emphasised responsible pet ownership and a right to life, even for feral animals, advocating for alternatives such as trap–neuter–release. Conclusion: New Zealanders largely view euthanasia as an ethically acceptable option for terminally ill or suffering animals but reject it when driven by convenience or financial hardship. Attitudes vary across demographic groups, highlighting the need for culturally sensitive education and policy. The findings align with Sustainable Development Goal 3 (Good Health and Well-being) and support broader discussions on responsible pet ownership, ethical decision making, and animal welfare legislation.
]]>Authors: Karen E. Griffin Claudia M. Vinke
Assessing dogs’ quality of life (QoL) pre- and post-adoption is crucial for shelters and rehoming organisations making decisions with far reaching and dire consequences for both dogs and humans. Making these assessments objectively and in a standardized way is important. As a first step to accomplish these two imperative goals, an assessment tool was developed based on the Framework of Dogs’ Needs, a robust model of needs at a species, breed/breed type, and individual levels based on scientific literature and expert opinion. It qualifies dogs’ QoL as a function of how well their needs are met. Because this tool will be used in real world situations, ensuring it is feasible and comprehensible by a non-scientific audience is key. Therefore, the three-step assessment tool was piloted with a dog owner sample (n = 14): a participant-completed questionnaire, an investigator given questionnaire with video chat observations (of the dog and their home environment), and participant recorded videos (of the dog displaying specific behaviours and scenarios). Participants then completed a feedback gathering survey on the entire process. Issues and aspects to change were also noted throughout by the principal investigator. Most issues pertained to the participant recorded videos, particularly regarding recording feasibility. Most participants said the collective time and effort involved in the assessment procedure would not discourage them from participating. Necessary changes were made to the tool based on all feedback and issues noted. It is now ready for usage in a larger project to assess dogs’ QoL throughout the sheltering and rehoming process.
]]>Authors: Sara E. Martini Patrícia M. Oba Jan S. Suchodolski Kelly S. Swanson
Antibiotics may be used for gastrointestinal enteropathies but research has demonstrated significant microbiota dysmetabolism, fermentation pattern alterations, and prolonged dysbiosis following treatment. The objective of this study was to determine how dietary fiber or fecal microbial transplant (FMT) treatments impacted the fecal characteristics, metabolite concentrations, and microbiota populations of cats treated with metronidazole. Twenty-five healthy adult cats (6.75 ± 1.20 yr) were fed a commercial kibble diet for 2 wk, administered metronidazole (20 mg/kg BW BID) for 2 wk, then monitored for 4 wk. Cats were allotted to one of three interventions (diet, diet + beet pulp, diet + FMT) for 1 wk, interventions ceased, then recovery was monitored for 4 wk. Fresh fecal samples were collected at the end of each phase and at the mid-points of recovery. As anticipated, metronidazole increased fecal scores and moisture (p < 0.05), reduced fecal bacterial alpha diversity (p < 0.0001), and reduced fecal metabolite concentrations. Few treatment effects were detected, with antibiotic recovery contributing to many of the results observed. Dysbiosis was persistent throughout the study, with 4/25 cats still demonstrating mild dysbiosis after 9 wk. Overall, dietary or FMT treatments may aid in accelerated antibiotic recovery in cats but further research is needed to refine treatments for greater efficacy.
]]>Authors: Iván Gómez Álvarez José Manuel Verdes García Luciano Espino López
Intervertebral disc disease is the most common disease of the spine in dogs and is a main cause of pain and neurologic dysfunction. This article reviews fundamental aspects of the pathophysiology, clinical presentation, diagnosis, and treatment of disc extrusions. Chondroid metaplasia of the nucleus pulposus is the central mechanism of disc degeneration. The clinical presentation varies considerably, depending on the breed, the location of the disc extrusion, and the degree of neurological damage. Advanced imaging techniques, such as computed tomography and magnetic resonance imaging, have greatly improved diagnosis, with magnetic resonance considered the gold standard. As for treatment, both medical and surgical management are effective options, depending on the degree of neurological damage and the initial response to conservative treatment. This comprehensive analysis underlines the importance of a multidisciplinary approach to optimize the quality of life of patients affected by intervertebral disc disease.
]]>Authors: Yvet Opmeer Stefanie Veraa Simon Platt Paul Mandigers
Background: Hereditary immune-mediated polymyositis has been reported in the Kooiker dog breed, associated with a 39 kb deletion and low penetrance. Approximately 10–20 percent of homozygous dogs and 0.5–2 percent of heterozygous dogs develop polymyositis. This study examines whether magnetic resonance imaging (MRI) can assist in diagnosing polymyositis in this breed. Methods: All dogs in this prospective case study were purebred Kooiker dogs referred for clinical examination to assess them for polymyositis. A dataset was compiled, including sex, neuter status, and, if applicable, age of onset, clinical signs, CK activity, electromyogram, and histopathological findings. MRI was performed using a 1.5 Tesla MRI scanner, with T1-weighted, T2-weighted, T2W fat-suppressed short tau inversion recovery (STIR), and T1-weighted post-contrast sequences. Results: Five Kooiker dogs were included in the study. Four dogs exhibited clinical signs compatible with polymyositis (one heterozygous and three homozygous for the 39 kb deletion), while one dog was homozygous for the 39 kb deletion but showed no clinical signs. The clinically affected dogs exhibited T2-weighted, STIR, and T1-weighted post-contrast muscular hyperintensity, and the diagnosis was confirmed with histopathology. The asymptomatic dog displayed no MRI abnormalities. Conclusions: MRI has proven to be a valuable tool in assisting with the diagnosis of Kooiker dogs carrying the 39 kb deletion. MRI can act as a screening tool for dogs with the 39 kb deletion, eliminating the need for an initial biopsy. A muscle biopsy, following a confirmatory MRI, is still the preferred method for diagnosing polymyositis.
]]>Authors: Aubrey Fine Zenithson Ng Matthew Knight Taylor Griffin Lindsey Braun Steven Feldman
Today, the pet effect is a powerful symbol for advancing the understanding and acceptance of the human–animal bond in societal and policy arenas. The primary aim of this commentary is to clarify the concept of the “pet effect” and demonstrate how advocates for this bond can leverage its influence to promote meaningful change and inform public policy.
]]>Authors: Cushla Redmond Ina Draganova Rene Corner-Thomas David Thomas Chris Andrews
Environmental enrichment is crucial to improve welfare, reduce stress, and encourage natural behaviours in dogs housed in confined environments. This study aimed to use accelerometery and machine learning to evaluate the effect of different enrichment types on dog behaviour. Three enrichments (food, olfactory, and tactile) were provided to dogs for five consecutive days, with four days between each treatment. Acceleration data were collected using a collar mounted ActiGraph®. Nine behaviours were classified using a validated machine learning model. Behaviour and activity differed significantly among the dogs. Dogs interacted most with the food enrichment, followed by the olfactory and then tactile enrichments. The dogs were least active during the olfactory enrichment, whereas activity was relatively consistent during the food and tactile enrichments. For all enrichments, dogs exhibited the most exploratory/locomotive behaviour during the first hour of each enrichment period, but this declined over the treatment period indicating habituation. For exploratory and locomotive behaviour, food enrichment was the most stimulating for the dogs with longer daily engagement than for both olfactory and tactile enrichments. These results illustrate that accelerometery and machine learning can be used to evaluate enrichment strategies in dogs, but it is important to consider variation among dogs and habituation.
]]>Authors: Arthenise Gabriely da Conceição Gomes Kayo Murilo Almeida de Souza Cruz Morais Nilsa Duarte da Silva Lima Regina Tie Umigi José Teodoro de Paiva Gisele Maria Fagundes
Canine obesity, characterized by the excessive accumulation of adipose tissue, is one of the most prevalent nutritional disorders encountered in veterinary practice. This comprehensive review synthesizes current knowledge on the predisposing factors and methodologies for assessing body condition in dogs. The discussion encompasses genetic, breed-specific, age-related, lifestyle, dietary, hormonal, and owner-related influences contributing to obesity. Key methods for assessing body condition, including the Canine Body Mass Index (CBMI), Body Condition Score (BCS), Relative Body Weight (RBW), and body fat percentage estimation, are critically evaluated for their effectiveness and clinical utility. This review aims to serve as a valuable resource for veterinary professionals and researchers dedicated to the prevention and management of canine obesity, highlighting both established practices and areas needing further investigation.
]]>Authors: Joana Eisinger Franziska Kuhne
Associations between age-related diseases and behavioral alterations have been highlighted in previous studies. This study investigates the prevalence of diseases and behavioral changes in non-diseased and diseased senior cats before and after diagnosis, concentrating on four prevalent diseases: 1. osteoarthritis, 2. chronic kidney disease, 3. hyperthyroidism, and 4. cognitive dysfunction syndrome. An online survey was performed by 594 German cat-owners with a cat older than nine years; prevalent diseases, related medications, and scaled behavioral questions before and after diagnosis were queried. Chi-Quadrat-Test and Spearman’s rank correlation were used to detect correlations between behavioral changes and diseases. Multiple linear regression was used to determine dependencies between behavioral changes and each disease pre- and post-diagnosis. Half of the cats had at least one disease diagnosed (54.6%). The most prevalent diseases were osteoarthritis (18.9%), chronic kidney disease (12.3%), and hyperthyroidism (8.9%). Cognitive dysfunction syndrome was diagnosed in 2.9% of the cats. With increasing age, the likelihood of developing at least one disease rose (rs = 0.204, p < 0.001). Disease-associated behavioral changes were found in the four mentioned diseases, with some behavioral changes occurring before diagnosis. These findings underscore the relevance of early detection of underlying diseases to decelerate ongoing behavioral changes in treatable diseases.
]]>Authors: Ashleigh R. Hunter Pamela J. Murison
The choice of what to feed is a significant decision for a pet owner and is complicated by the vast array of choices that are available. The purpose of this study was to investigate the feeding choices and practices of UK dog and cat owners. An online survey was conducted in which owners were asked about their feeding practices, what they look for in pet food, and purchasing habits, as well as collecting owner demographics and pet signalment information. A total of 520 responses were collected for 682 dogs and cats (572 dogs and 110 cats), with most owners only having one pet. Significantly more cats were fed a mix of wet and dry food or solely wet food, while significantly more dogs were fed raw food. Most owners feed their pets twice a day in a standard bowl and obtain nutritional information online. Furthermore, the most common way to purchase their food is online. There are differences between cat and dog owners feeding choices, with cat owners staying with more traditional methods and more dog owners choosing raw or alternative feeding. Raw feeding has increased in popularity compared to previous studies.
]]>Authors: Sara E. Martini Teresa Schmidt Wenyi Huang Amanda B. Blake João P. Cavasin Jan S. Suchodolski Kelly S. Swanson
Antibiotics are commonly used to aid in the remission of gastrointestinal diseases, but usage may lead to prolonged dysbiosis. The objective of this study was to evaluate the effects of metronidazole on fecal microbiota, fecal metabolites, and serum bile acids and uremic toxins of healthy adult cats. Twelve healthy adult cats (4.7 ± 0.4 yr) received metronidazole (20 mg/kg BW PO BID) for 14 days (day 0–14) and were monitored during a 28-day recovery period (day 15–42). Fecal and blood samples were collected at baseline (day 0), after metronidazole (day 14), and weekly during recovery (on days 21, 28, 35, and 42). Fecal samples were analyzed for microbiota and bacterial metabolites. Serum samples were analyzed for bile acids and uremic toxins. Metronidazole increased dysbiosis index and fecal lactate concentrations (p < 0.0001) and decreased fecal propionate, butyrate, and secondary bile acid concentrations (p < 0.0001) for up to 28 days. Prolonged dysbiosis and Peptacetobacter (Clostridium) hiranonis reductions were observed in 10/12 (83%) cats. Serum uremic toxins were also reduced (p < 0.0001) after metronidazole administration. The observed changes after metronidazole administration illustrate how changes in the gut microbiome alter microbial metabolism and its relation to host dysmetabolism. In conclusion, metronidazole is a potent antibiotic with persistent effects observed in the microbiome and metabolome, even up to one month after administration.
]]>Authors: Amanda B. Blake Linda C. Toresson Chih-Chun Chen Patricia E. Ishii Robert Kyle Phillips Paula R. Giaretta Joao P. Cavasin Jonathan A. Lidbury Jan S. Suchodolski
Bile acids (BAs) are important signaling molecules in the gastrointestinal (GI) tract and are associated with health and disease in humans and animals. Intestinal bacteria transform BA through deconjugation, dehydroxylation, and epimerization reactions, producing various isoforms, many of which have not been investigated in companion animal diseases. We aimed to develop and analytically validate a novel liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the quantification of 30 BAs in dog feces, with a simple extraction procedure and on-line solid-phase extraction. Validation demonstrated good accuracy, precision, sensitivity, spiking recovery, dilution, and stability for 29 BAs. The method was applied to fecal samples from healthy dogs (H; n = 121) and dogs with chronic enteropathy (CE; n = 58). The immediate and downstream products of bacterial 7α-dehydroxylation reactions with cholic acid were lower in concentration in dogs with CE when compared to healthy dogs (deoxycholic acid, 3-oxo-deoxycholic acid, and 12-oxo-lithocholic acid; q < 0.001). Across all fecal samples, the products of hydroxysteroid dehydrogenase (including oxo- and iso-BA) made up an average of 30% of the total measured fecal BA pool (glycine-BA, 0.1%; taurine-BA, 2.2%; unconjugated BA, 53%).
]]>Authors: Rachel Gilchrist Clive D. L. Wynne
Many stressors have been identified for shelter dogs, and while stress is a natural physical response, exposure to shelter stressors has been implicated in poor dog welfare including the formation of maladaptive behaviors that manifest as unappealing responses toward humans. Inappropriate behavioral reactions to humans lead to longer lengths of stay in the shelter, so identifying ways to reduce these responses to human presence would not only benefit the immediate welfare of the dog but could also decrease the amount of time the dog spends in the shelter. Previous studies have shown that response-independent food paired with an auditory stimulus reduced behaviors that likely prolong time to adoption; however, these interventions used an explicit discriminative stimulus where a more easily deployed design would be effective without requiring an additional stimulus to precede the food. The purpose of the present study was to extend that work to evaluate the effect of noncontingent reinforcement on reducing behavioral reactions to humans in the presence both of the experimenter and other people. Overall, the intervention was effective at decreasing human-directed behavioral responses but did not decrease the dogs’ length of stay. Changes in reactive behaviors appear to be limited to the presence of the experimenter during the first and last trials, with improvements generalizing to non-experimenters only for two behaviors: staying in the front half of the kennel and facing the kennel front. Our recommendation for shelters is that this intervention would be most effective at improving behaviors directed at a person delivering treats to the dog.
]]>Authors: Francesca Brugnoli Barbara Simionati Ilaria Patuzzi Angiolella Lombardi Maria Cecilia Giron Edoardo Savarino Sonia Facchin Giada Innocente
Background: In veterinary medicine, fecal microbiota transplantation (FMT) shows promise for treating chronic enteropathy (CE) in dogs, but standardized protocols for dosage, preparation, and administration are lacking. This study aimed to evaluate the efficacy of freeze-dried FMT capsules (cFMT) and to investigate the existence of a possible optimal dosage for dogs with CE. Methods: A multicenter prospective study was conducted on 171 dogs with CE, treated with freeze-dried FMT capsules (100 mg for dogs ≤ 10 kg, 200 mg for dogs > 10 kg). The dosage of freeze-dried FMT material was expressed in different ways, to investigate the effect of putative active principles. Clinical outcomes were assessed by classifying dogs as responders (R) or non-responders (NR) based on veterinary evaluations from a questionnaire, along with changes in the CIBDAI score and variations in 15 clinical signs of chronic enteropathy (CE). Data were collected before and 15 days after treatment. Results: Of the 111 dogs included in the final analysis, 82% showed a positive clinical response, with no significant differences in clinical response between capsule sizes or dosage, irrespective of how it was expressed. Conclusion: Effective dosage range for cFMT administration in dogs affected by CE was defined. The oral administration of 100 mg of freeze-dried cFMT daily for a month was shown to be sufficient to achieve an 80% response rate. Further studies are needed to explore additional factors that may influence the overall effectiveness of cFMT in treating CE.
]]>Authors: Alice Diana Damjan Mickov Rens van Dobbenburgh Wiebke Jansen Susanna Sternberg Lewerin Stephan Neumann Mette Uldahl Nancy De Briyne
In recent years, the European companion animal veterinary care market has experienced significant growth, with 166 million households (50%) owning one or more of Europe’s 352 million companion animals. This expansion is accompanied by a notable market trend toward the corporatisation of veterinary practices, particularly in urban areas and certain countries. Corporatisation has reshaped the profession, offering benefits such as economies of scale, advanced veterinary treatments, and structured career pathways for early-career veterinarians. However, this shift also prompted discussions on the impact it has on rising costs of care, professional autonomy constraints, and potential reductions in rural practice attractiveness. Our comprehensive study, combining a large-scale survey of European veterinarians, semi-structured questionnaires, and an extensive literature review, maps the current state of corporatisation in Europe, identifies key players, and examines its impact on the veterinary profession and broader society. The findings underscore the rapid expansion of corporate veterinary practices and the accompanying competitive pressure on independent vet-owned clinics. To ensure a sustainable future for the profession, our research highlights the need for further investigation to fully comprehend the effects of corporatisation. Addressing these challenges is essential to protect the interests of animals and their caretakers, veterinarians, and the wider society.
]]>Authors: Ryan Guldenpfennig Clare Hsu Krysten Fries-Craft Adriana Garber Xinhe Huang Mark Wieneke Kristen Rutledge
Plant extracts, such as olive extract (OE), have been used in human and pet foods for their biological benefits; however, no available data have demonstrated OE’s effect on palatability in dogs. The current study aimed to evaluate acceptance of dry and canned dog foods with differing inclusions of OE as a flavor component. Flavor compounds in OE were analyzed by gas chromatography–mass spectrometry and high-pressure liquid chromatography, detecting 137 volatile compounds, including acetic acid and hydroxytyrosol. Dog kibbles were coated with liquid commercial palatants containing OE that resulted in application rates of 0 (control), 120, 200, and 500 ppm of OE in the diets. OE was also added at 0 (control), 120, 200, and 500 ppm into a wet food formulation with a commercial palatant before retort processing. Two separate panels of adult beagles were used for monadic testing to determine acceptance rates for kibble (5/treatment; 20 total) and canned foods (4/treatment; 16 total) in a 4 × 4 Latin square design. None of the tested inclusions impacted food acceptance in this preliminary study (p > 0.05). As a flavor ingredient, OE can be added into dry or wet dog food up to 500 ppm without deterring effects on palatability.
]]>Authors: Catherine Kokemuller Ryan Guldenpfennig Clare Hsu Krysten Fries-Craft
Olive extract (OE) has been used in human foods for its nutraceutical effects, making it a product of interest for pet food. However, OE’s effect on palatability has not been examined. The study objective was to evaluate the palatability of dry cat foods with OE applied at differing inclusions within liquid or dry palatants. Twenty-seven volatile compounds were identified by gas chromatography–mass spectrometry for a potentially earthy or fruit-like flavor profile. Liquid palatants were formulated to supply 0 (control), 15, 30, 50, 75, and 150 ppm OE, and dry palatants were formulated to provide 0, 100, 200, 400, and 600 ppm OE when coated onto kibble. Palatability was evaluated using two-day, two-bowl testing of OE-containing versus control rations in adult cats (n = 20) with two-tailed t-tests to determine if OE affected intake ratio (IR). The observed IR of rations with OE were 0.45 to 0.56. The only preference was the 200 ppm treatment (IR = 0.56; p = 0.01) while the other OE rations were not different from the control (p ≥ 0.05). These findings indicate that palatant formulations can supply kibble diets with up to 150 ppm OE for liquid and 600 ppm for dry applications without negatively impacting cat food palatability.
]]>Authors: Lena Kreuzer Anna Naber Roswitha Zink Lisa Maria Glenk
Children with psychosocial, developmental or physical impairments benefit from equine-assisted therapy (EAT) in multiple ways. However, to date, the animal perspective of such interventions has received comparatively less scientific dedication. Thus, heart rate (HR) and heart rate variability (HRV) of seven therapy horses that lived in an open stable environment and participated in therapeutic group sessions with children were monitored within 60 min prior to and within 90 min after EAT. Moreover, cardiovascular activity was compared to a control condition on a day without any EAT sessions. No significant differences in HR or HRV were found, neither before nor after EAT nor under the control condition. These findings do not give rise to any concern regarding horse welfare in the studied setting but cannot be generalized to a broader population of equines serving in EAT. Given the enormous heterogeneity in EAT, more in-depth research is warranted using behavioral and physiological indices of equine health and wellbeing.
]]>Authors: Christine Smetaczek Birgit Ursula Stetina
Human–animal relationships have long been idealized as universally positive, but recent studies highlight the complexities and contradictions inherent in this bond. This study re-analyzes previously published data to examine the nuanced effects of pet ownership on human well-being, particularly under stressful circumstances such as the COVID-19 pandemic. Using a mixed-methods approach, data from three independent surveys (n = 840) were re-analyzed to explore the bio-psychosocial implications of pet companionship. Key instruments included the Quality of Life Questionnaire (WHOQOL-BREF), Beck Depression Inventory (BDI-II), and Lexington Attachment to Pets Scale (LAPS). Results revealed that pet ownership was significantly associated with higher levels of psychosocial stress, challenging the assumption that pets universally improve quality of life. Notably, individuals with current pet ownership reported lower quality of life compared to non-owners. However, qualitative analyses illustrated perceived emotional and social support among pet owners, reflecting the “pet-effect paradox.” Mental health variables such as depression and loneliness emerged as stronger predictors of well-being than pet attachment. This study introduces the “Aunty Phenomenon” as a conceptual framework to describe the idealization of pet relationships, while acknowledging the responsibilities they entail. These findings underscore the importance of a holistic approach to human–animal bond research.
]]>Authors: Lincy Koodaly Erika Friedmann Nancy R. Gee Eleanor M. Simonsick Barbara Resnick Eun-Shim Nahm Elizabeth Galik Sarah Holmes
Background: The growth of the older adult population calls for innovative and cost-effective ways of promoting their physical, psychological, and cognitive health. Human–animal interaction, including pet ownership, is related to positive and negative aspects of human health. Not all pet owners respond in the same way. The levels of pet attachment and pets’ influence on their owners’ lives could moderate the relationship between psychological status and health outcomes. Purpose: We examined the moderating role of pet attachment in the relationships of psychological status (mental wellbeing, happiness, anxiety, depression) to physical function (physical wellbeing, usual- and rapid-gait speeds, physical performance battery) in community-residing older adult pet owners. Methods: A cross-sectional, secondary analysis of pet-owning older adult participants in the Baltimore Longitudinal Study of Aging (n = 178). Results: In regression analyses, controlling for age, gender, and comorbidities, pet attachment and pet influence moderated the relationships of physical wellbeing to mental wellbeing and anxiety (p < 0.05). Pet influence also moderated the relationship between anxiety and usual gait speed (p < 0.05). Conclusions: Greater attachment and influence buffer the relationship of perceptions of poor mental function with perceptions of poor physical wellbeing suggesting one mechanism for health benefits of human-animal interaction.
]]>Authors: Victoria Wessely Jan S. Suchodolski João P. Cavasin Mara Holz Kathrin Busch-Hahn Stefan Unterer
Several recent studies have reported a significantly greater prevalence of Clostridium perfringens encoding the novel pore-forming netF toxin gene in dogs with acute hemorrhagic diarrhea syndrome. However, the presence of netF in other canine diarrheal diseases remains poorly characterized. This retrospective, cross-sectional study aimed to describe the prevalence and abundance of netF-positive C. perfringens in fecal samples from 352 dogs with acute and chronic gastrointestinal diseases. Dogs were divided into five groups: acute hemorrhagic diarrhea syndrome (AHDS), acute diarrhea (AD), chronic enteropathy (CE), exocrine pancreatic insufficiency (EPI), and healthy controls (HCs). The abundances of C. perfringens 16S rRNA, the C. perfringens enterotoxin gene and the C. perfringens netF gene in fecal samples were analyzed by quantitative polymerase chain reaction. In total, 7 of 15 (46.7%) dogs with AHDS, 10 of 75 (13.3%) dogs with AD, 2 of 120 (1.7%) dogs with CE, 1 of 12 (8.3%) dogs with EPI, and 1 of 130 (0.8%) HC dogs tested positive for netF. This study provides further evidence that NetF may be a significant contributor to the etiology of AHDS and potentially to a subset of acute nonhemorrhagic diarrhea cases, while it was only rarely detected in chronic gastrointestinal disease phenotypes.
]]>Authors: Alexa M. Carr Patricia Pendry
(1) Each year, thousands of students leave their pets behind to attend university, often causing separation anxiety and losing a vital stress-coping resource. While many universities offer animal visitation programs (AVPs), their effectiveness in supporting student wellbeing during this transition remains unclear. This randomized controlled trial evaluated psychological mood risk and resilience in a randomly selected sample of first-year university students (n = 145) separated from their childhood pets. (2) Participants were randomly assigned to receive access to a seven-session, biweekly 2 h drop-in program (n = 77) featuring unstructured interactions with therapy dogs or a waitlist control group (n = 68). Assessments of wellbeing were conducted at the start, middle, and end of the semester including depression, anxiety, worry, stress, cognitive reappraisal, expressive suppression, and self-compassion. (3) Regression analyses showed that access to the semester-long drop-in program significantly flattened trajectories of depression (B = −3.05, p = 0.01, d = 0.514), worry (B = −3.92, p = 0.04, d = 0.416), and stress (B = −1.94, p = 0.05, d = 0.386) compared to the control group. Students in experimental conditions also showed improvements in self-compassion (B = 4.03, p < 0.001, d = 0.605). (4) These findings suggest regular access to unstructured drop-in programs featuring therapy dogs may provide valuable psychological support for students adjusting to university life.
]]>Authors: Nils Altner Laura Kemmling Zaida Rentería-Solís Anna Obiegala Simone A. Fietz Romy M. Heilmann
Toxoplasma gondii is a protozoan parasite in cats that can be a cause of zoonotic concern due to sheding infectious and highly resistant oocysts. Clinical toxoplasmosis is rare in cats and can occur with mild to severe clinical signs or even fatal outcomes. T. gondii immunoglobulin M (IgM) or IgG is an indicator of ongoing or prior infection. Information about T. gondii occurrence in the domestic cat population, primary clinical concerns about affected cats, and potential risk factors is scarce in Germany. This retrospective study explored data from 174 feline patients (≥12 weeks old) presented to a tertiary veterinary care facility in central Germany between 2012 and 2023. T. gondii serological status, determined as part of the cats’ routine diagnostic evaluation, was assessed in relation to patient characteristics, environmental factors, medical history data, and clinical diagnosis. Neurological (57%), locomotor (25%), and ocular signs (11%) were common, and older age was associated with the presence of IgG (p = 0.008). Sex had no significant effects on T. gondii antibody positivity (p = 0.865). More outdoor cats than indoor-only cats were T. gondii IgG-positive (p = 0.021), and IgG-seropositive animals received a canned/wet diet more often than a dry kibble diet (p < 0.0001). Clinical representations aligned with previous studies, and age, choice of food, and outdoor living (p = 0.021) were significant predisposing factors.
]]>Authors: Aviva Vincent Mary Rauktis Laurie Maxwell Elizabeth Knudsen
Animal shelters play a pivotal role in protecting pets in crisis and bringing families together. This paper adds to the work of Hoy-Gerlach and colleagues which outlined a three-tier framework for developing and training veterinary social workers (VSWs) to serve in animal shelters. Our research questions sought to explore the VSWs’ self-identified role (RQ1), and how VSWs self-identify their scope of practice (RQ2). Through these exploratory questions, our research aimed to advance knowledge about the state of VSWs in the animal-shelter setting. Methods: Using a purposive sampling strategy, an anonymous community practice survey (CPS) was emailed to VSWs working in animal shelters. Inclusion criteria included being over the age of 18, being currently employed in an animal shelter, and cognitive fluency in English. The survey yielded 25 responses with 14 included in the data analysis. Findings: The VSWs identified their role as supporting multiple groups, including clients and their practice community (i.e., colleagues). While most of their time was devoted to individuals from the community, they also supported the mental wellbeing of their team. Their work ranged from case management and psychoeducation to crisis intervention and suicide risk assessment. As there are no standards of practice for VSWs, each respondent used unique language to explain their role and scope of practice. Conclusions: The CPS was helpful in illuminating developing trends within the field, though a larger sample size is needed to gain a more nuanced understanding of self-identified needs of VSWs practicing in animal shelters and other related spaces.
]]>Authors: Nicolas Dollion Jennifer R. Babka Kelly E. Pollock Julie A. Stilley Marine Grandgeorge Gretchen Carlisle
Service dogs (SDs) have been shown to provide multi-faceted benefits for autistic children, with some benefits also extending to family members. The main objectives of this study were to explore the relationships between psychosocial well-being, family functioning, parent anxiety, and parent–child relationships in families of autistic children, and to investigate the effects of integration of an SD. A cross-sectional approach was used to collect data from three groups of parents of autistic children: (1) families approved for an SD but waitlisted, (2) families in the training process but not yet paired with an SD, and (3) families with a child matched with an SD. Parents completed an online questionnaire including validated tools. Children’s autism-related symptoms correlated with different aspects of family experience and quality of life, and parents’ anxiety scores were related to children’s social anxiety and difficulties associated with parenting an autistic child. Family functioning was associated with children’s autistic characteristics, parents’ anxiety, and the quality of the parent–child relationship. In families of children paired with an SD, children’s autistic symptoms were related to parental attachment with the SD. These findings underscore the need for further research on the assessment and implications of autism-related symptoms for children acquiring an SD.
]]>Authors: Ronald Jan Corbee Patrick van Hoorn Paul A. M. Overgaauw
Background: The Dutch branch organization for pet products promised the public that it will improve the quality of raw meat-based diets (RMBDs) for dogs after several diagnoses of tuberculosis, brucellosis, and hyperthyroidism in dogs fed RMBDs. Objective: The objective of this study was to re-evaluate the risk factors of commercially available raw meat diets for dogs in The Netherlands. Methods: Seven commercial brands of RMBDs that were previously investigated were re-tested, as well as a newly introduced high-pressure processing (HPP) product. Raw beef sausage for humans was included for comparison. In total, 40 animal RMBDs (five batches per product) were tested for the presence of colony-forming units (CFUs), Salmonella spp., and Escherichia coli directly after defrosting and 4 h later, as well as thyroid hormone. Results: Exceeded EU standards for CFUs and Salmonella bacteria were present in several samples. In the HPP product, bacteria were still present; however, the counts were lower. There were no differences in CFUs directly after defrosting and 4 h later. The human raw meat product was negative for bacteria. Thyroid hormone could be detected in 20 out of 37 samples. In seven of these samples, the levels were >0.75 µg/g, which have been associated with hyperthyroidism. Conclusions: The hygiene (including the use of HPP production) and accurate removal of thyroid tissue during the production of RMBDs still need attention to prevent the presence of zoonotic bacteria, high CFUs, and diet-induced hyperthyroidism.
]]>Authors: Marianne Pan Chi-Hsuan Sung Rachel Pilla Jan S. Suchodolski Stacie C. Summers
The purpose of this study was to assess the practical implications of supplementing soluble fiber in the diet of dogs. Dogs with a history of managed or active chronic enteropathy were randomized to receive either wheat dextrin (fiber group) or maltodextrin (placebo group) mixed with food once daily for 28 days. Owners recorded a daily fecal score one week prior to and during the supplementation period. Shallow shotgun sequencing, quantitative PCR abundances of core bacterial taxa, and short-chain fatty acid (SCFA) concentrations via gas chromatography/mass spectrometry were performed on fecal samples collected before and after supplementation. Seventeen dogs completed the study (fiber group: nine dogs; placebo group: eight dogs). The change in fecal score differed between groups, with the fiber group developing softer stools (p = 0.03). Alpha diversity, quantified PCR abundances of the SCFA-producing taxa, and fecal SCFA concentrations were not different after supplementation in either group. Fecal microbial communities differed between baseline and day 28 for fiber and placebo groups (p = 0.02, respectively); however, the size effect (ANOSIM R = 0.18 and R = 0.26, respectively) was minimal. In this small group of dogs fed variable commercial diets, the additional intake of wheat dextrin powder supplement was well accepted, but had minimal discernable clinical benefit, and could soften stools.
]]>Authors: Tiffani J. Howell Silvana Diverio David J. Menor-Campos
Despite an increase in research into cat and dog cognition, behavior, and welfare in recent decades, it is unclear whether pet owners are aware of recent advances in our understanding of cats and dogs. Misunderstandings about the reasons for animal behavior can lead to negative welfare outcomes for the animal, and potentially distress for the owner, so it is important for owners to understand the latest science on animal behavior. Current and former pet owners (N = 224) completed an online survey rating their agreement with a series of statements about cat and dog behavior. After completing the survey, participants were provided with a document describing the latest scientific knowledge about each of the statements in the survey. For both species, participant beliefs generally accord with the latest scientific knowledge, but there is evidence of remaining beliefs about aspects of dominance training theory in dogs, and the belief that cats are low-maintenance pets. These findings can be used by trainers and behaviorists to help educate owners about pet needs, working from the owner’s baseline knowledge and debunking persistent myths.
]]>Authors: Christie Siettou Eleni Theodoropoulou Anna Stefani Siettou
This study provides an overview of the new Greek legislative framework and highlights its progressive nature in tackling one of the most populous stray animal populations in the world. We present the newly established law and discuss its provisions, aims, and challenges. We also examine the reception of the law by key stakeholders such as veterinary practitioners, animal welfare organisations, and the Hellenic Kennel Club. With the post-implementation review scheduled for 2026, its evaluation has yet to be conducted.
]]>Authors: Joanna Lin Christina Schwens Natali Bauer Katarina Hazuchova
Adult-onset spontaneous feline hypothyroidism (SH) is considered rare, but its prevalence is unknown. This study aimed to screen laboratory submissions for cats with laboratory suspected SH (LSSH) and to identify laboratory abnormalities associated with LSSH. Submissions to a commercial laboratory in Germany between January 2022 and April 2023 were prospectively screened for cats aged 3–12 years with low total thyroxine (TT4); in these cats, thyrotropin (TSH) was measured. Iatrogenic hypothyroidism was excluded by contacting submitting veterinarians. Creatinine, triglyceride and cholesterol concentration and red blood cell count (RBC) of cats with LSSH (low TT4, TSH > 0.53 ng/mL; i.e., [one sided TSH reference change value (76%) of TSH upper reference interval (RI)] + [TSH upper RI] = 0.53) were compared to euthyroid cats (TT4 within RI) and cats with suspected non-thyroidal illness (SNTIS) (low TT4, normal TSH (<0.3 ng/mL; upper RI)) by non-parametric tests. p < 0.05 was significant. In total, 31,572 submissions of cats were included, 25,169 (79.7%) were euthyroid, 3818 (12.1%) had SNTIS and 61 (0.2%) had LSSH. Cats with LSSH had higher creatinine (p = 0.002) and lower RBC count (p < 0.0001) than euthyroid cats as well as higher creatinine (p = 0.00035) than cats with SNTIS. Azotaemia (creatinine > 140 µmol/L) and anaemia (RBC < 7.2 × 1012/L) were present in 28/49 (57%) and 19/44 (43%) cats with LSSH, respectively. There was no difference between LSSH and SNTIS for the remaining parameters. In cats with low TT4, azotaemia and anaemia might indicate the presence of SH and reinforce the need for TSH testing. Hypercholesterolaemia and hypertriglyceridaemia are not indicators of SH.
]]>Authors: Isabelle C. Jeusette Emmanuelle Apper Marina L. Meli Regina Hofmann-Lehmann Anna Salas-Mani Anna Fernández-Pinteño Olga Francino Celina Torre
Twenty-six cats were fed either a control or an inulin-supplemented diet (0.6%) for six weeks with measurements of fecal short-chain fatty acids (SCFAs) and microbiota. In the second phase, cats were fed their respective diets for another six weeks and were then primo-vaccinated against the feline leukemia virus (FeLV) at days 0 and 21. Serum anti-FeLV IgG responses were monitored. Compared to the control group, the inulin group exhibited higher Firmicutes (+14%) and lower Bacteroidetes (−56%) and Prevotellaceae (−51%) after six weeks of supplementation. Compared to T0, SCFAs initially decreased at week 3 but subsequently increased at week 6 with inulin supplementation, leading to higher butyrate compared to the control group at week 6. A significant diet-by-time interaction was also observed for propionate and total SCFAs. Compared to the control group, the inulin group tended to show a higher serum anti-FeLV IgG response after the first vaccination (p = 0.09), with significant differences at days 5 and 11 post-vaccination. These preliminary results suggest that a six-week dietary supplementation with a low dose of inulin in cats can modify the fecal microbiota and its functional metabolites, potentially influencing the early immune response to vaccination.
]]>Authors: Rowena M. A. Packer Anna Wade Johanna Neufuss
The popularity of brachycephalic dogs has increased worldwide despite growing evidence of their profound health and welfare issues, largely as a result of their extreme conformation. This study aimed to evaluate the prevalence and risk factors for known perceptual barriers to improving their welfare. An online survey of n = 2006 UK dog owners was conducted, including owners of non-brachycephalic (non-BC; n = 964), mild-moderate brachycephalic (M-BC; n = 706), and extreme brachycephalic (E-BC; n = 336) dogs. The survey explored contemporary knowledge and attitudes toward brachycephalic dogs, with multivariable statistical analyses identifying risk factors for normalisation of brachycephaly-related abnormalities, preference for flat faces and ‘laziness’ in dogs, and self-reported intractability to being deterred from buying a brachycephalic breed. Almost 1 in 7 E-BC owners considered that there was ‘nothing’ that could dissuade them from buying a brachycephalic dog, predicted by a strong preference for flat faces as a highly desirable aesthetic characteristic and beliefs that brachycephaly did not negatively impact lifespan. E-BC owners were most likely to consider flat faces a highly desirable aesthetic characteristic, predicted by highly valuing ‘laziness’ as a behavioural characteristic. Preference for ‘laziness’ as a behavioural characteristic was predicted by low levels of pre-purchase research and aesthetic preferences (e.g., flat faces, large size). High levels of normalisation of brachycephaly-related abnormalities were observed across all ownership groups, particularly for clinical signs related to exercise intolerance, with these beliefs predicted by deficits in dog acquisition behaviours and knowledge, and intractability from being deterred from purchasing a flat-faced dog. These findings highlight the pervasiveness of perceptual barriers that inhibit campaigning regarding brachycephaly and the need for targeted interventions to address misconceptions (e.g., ‘myth busting’ laziness as a positive trait), while being mindful of the heterogeneity of beliefs within the brachycephalic-owning population, particularly the intractable sub-population to whom shifting preferences away from flat-faced conformations may prove impossible without stronger measures, e.g., legislative change.
]]>Authors: Tabitha Hookey Brianne Morrow Georgiana R. T. Woods Franck Péron John Flanagan Virginie Gaillard
The health of adult dogs and cats is affected positively or negatively by their development in utero and throughout postnatal growth. Preventive veterinary care is particularly important when animals are physiologically immature and sensitive to modifiable environmental factors. Veterinary nurses/technicians are often at the forefront of promoting healthy development, reinforcing the work of veterinarians and using their knowledge, experience, and passion to lead initiatives with breeders and pet guardians. This opinion article considers the role of qualified veterinary nurses/technicians in the care of puppies and kittens throughout their developmental life stages—gestation, the suckling period, growth post-weaning to puberty or neutering, and late growth to adulthood. Much of their influence is through the education of pet carers; they provide trustworthy information relevant to the individual pet and focused on the practicalities of best husbandry practices. These include practical advice on recognizing dystocia, neonatal care, weaning, introduction to the new home, the prevention and management of infectious diseases, socialization with conspecifics and humans, habituation to potential environmental stresses, nutrition, oral hygiene, and grooming. The veterinary nurse’s goal is not only to see young pets develop into healthy, well-adjusted adults but also to see pet guardians developing sustainable human–animal bonds.
]]>Authors: Shibin Ye Zhihao Cao Shiyan Jian Limeng Zhang Baichuan Deng Jinping Deng
Frozen meat is an important source of protein in pet food, and has attracted much attention in recent years. In this study, we compared the effect of meat meal (MM), frozen meat (FM), and hydrolyzed frozen meat (HFM) as ingredients in extruded pet food on its palatability and apparent digestibility, as well as its effects on the immune response, fecal microbiota, and metabolome of British shorthair cats. A total of 24 British shorthair cats were allocated to the MM, FM, and HFM groups according to body weight and gender. The palatability test lasted 4 days and the feeding test lasted 45 days. The results showed that the FM and HFM diets had better palatability than the MM diet (p < 0.05) and significantly improved dry matter and crude protein digestibility (p < 0.05). The serum IL-10 level was significantly higher in the HFM group compared to the MM and FM groups (p < 0.05). The serum IgM levels were also found to be significantly higher in the FM group compared to the MM and HFM groups (p < 0.05). The blood urea nitrogen/creatinine ratio was significantly lower in the HFM and FM groups than in the MM group (p < 0.05). Cats fed HFM had a higher abundance of fecal Actinobacteria and Bifidobacterium and a lower content of Bacteroidota (p < 0.05). Furthermore, serum metabolomics analysis revealed that the tryptophan (Trp) metabolism and bile acid metabolism were affected by HFM. Overall, FM and HFM were better for the cat’s health than meat meal, but they also have some potential risks.
]]>Authors: Elizabeth Ippolito Michael Merkhassine Jethro M. Forbes John P. Loftus
Vitamin D toxicosis poses a health threat to dogs, with cases often stemming from cholecalciferol rodenticide ingestion. This case report investigates two clinical cases of canine cholecalciferol toxicosis, shedding light on the persistent elevation of 25-hydroxyvitamin D (25(OH)D) and the adaptive response of 24,25-dihydroxyvitamin D (24,25(OH)2D). Serum samples from affected dogs were analyzed over several months, revealing sustained increases in 25(OH)D concentrations. Notably, concurrent measurements of 24,25(OH)2D unveiled a marked elevation, suggesting a compensatory mechanism to mitigate calcitriol excess and hypercalcemia. These findings highlight the potential role of upregulating 24-hydroxylase activity as a therapeutic target for managing cholecalciferol toxicosis. These cases underscore the importance of understanding vitamin D metabolism in canine toxicology and prompt further exploration into novel treatment strategies and other research areas.
]]>Authors: Lori R. Kogan Jennifer Currin-McCulloch Cori Bussolari Wendy Packman
Pets are often an integral part of the family structure, with many people viewing their pets as children and thinking of themselves as “pet parents”. Pets have been shown to provide an array of psychological and physical health benefits, but some research suggests that the effects of pet ownership are more nuanced than once thought. One common negative aspect of pet ownership is guilt. Drawing from research pertaining to the transition to siblinghood, this study explored pet owner guilt as a result of adding a child to a dog-owning family. It also measured changes in dogs’ behaviors and their impact on owners’ depression and anxiety levels. Results suggest that many parents feel dog-related guilt and struggle with the resultant changes in household dynamics with the arrival of a child. Suggestions to help improve the chances of a successful transition include awareness, preparation, and self-compassion. Planning and preparing can help minimize negative dog behaviors and, as a result, levels of parental depression and anxiety. Parents are encouraged to help their dogs succeed by taking the time to prepare beforehand, thereby minimizing their own guilt and decreasing the potential of a severed human–dog bond.
]]>Authors: Angela Matijczak Shelby E. McDonald Lori R. Kogan Rosalie Corona Katlyn Reynolds
Many LGBTQ+ emerging adults experience rejection from their family of origin. Family rejection is a stressor that contributes to worsened mental health for LGBTQ+ emerging adults. Supportive relationships with others can be an important protective factor for LGBTQ+ emerging adults as they cope with family rejection. The bond and interactions LGBTQ+ pet owners have with their pets may provide love, comfort, and support, similar to the relationship they may have with a human attachment figure. This quantitative study aimed to test pet attachment as a moderator of the association between family rejection and mental health in a sample of LGBTQ+ emerging adults. We collected survey data from 201 18–25-year-old LGBTQ+ pet owners living in the state of Virginia. After testing several moderation models using the PROCESS macro in SPSS, we found that pet attachment was not a significant moderator of the relation between family rejection and mental health symptoms (i.e., depression, anxiety, psychological distress). The findings suggest that pet attachment may not be a protective factor for LGBTQ+ emerging adults who experience family rejection, although these findings should be interpreted in light of the characteristics of our sample and limitations of the study. We recommend that future researchers explore alternative moderators or test these associations among LGBTQ+ individuals in other developmental periods.
]]>Authors: Luisa Mascarenhas Ladeia Dutra Angelica da Silva Vasconcellos Robert John Young
This study evaluated the potential of using dogs’ apparent age, judged from photographs, as a non-invasive tool for assessing their welfare. Traditional welfare assessment methods often rely on behavioral and physiological indicators, which can be resource-intensive and invasive. This research explored whether apparent age, a measure used in humans to predict health and longevity, can also serve as an indicator of welfare in dogs by investigating its association with relative telomere length (RTL), a biomarker of biological aging. Photographs of 60 domestic dogs were evaluated by canine specialists and general volunteers via the citizen science platform Zooniverse. Participants estimated the age of 20 dogs from three different age categories: young (0–2 years), adult (2–5 years), and senior (6+ years). The accuracy of these predictions was compared to the dogs’ chronological ages and RTLs. Generalized linear models were used to assess factors influencing prediction accuracy, including the dogs’ age, sex, and origin. Results indicated that both specialists and volunteers reliably estimated the age of senior dogs, with no significant differences in accuracy between groups. Dogs with accurate apparent age estimates had RTLs matching their chronological age, while those with premature aging signs had shorter RTLs. This suggests apparent age could be a practical, non-invasive welfare assessment tool, offering a potentially accessible method for new welfare assessment protocols.
]]>Authors: Claire L. Timlin Fiona B. Mccracken Sarah M. Dickerson Patrick M. Skaggs Jason W. Fowler Sangita Jalukar Craig N. Coon
Postbiotics are emerging as potential functional ingredients for companion animal diets. This study aimed to determine if a Saccharomyces cerevisiae-based postbiotic can alter cytokine and stress responses to exercise and transport stress in adult Labrador Retrievers. Dogs received 15 g ground corn germ (Control, n = 12), 7.5 g postbiotic (Low, n = 12), or 15 g postbiotic (High, n = 12), daily for 63 days. Exercise was twice weekly for 7 weeks, and a single transport per dog occurred in week 8. Fecal inflammatory biomarkers, serum chemistries, and complete blood counts were assessed at the beginning and end of the study. Serum cytokines were quantified before and 18–20 h after the first and last exercise runs. Gait analysis was assessed before and 24 h after the first and final runs. Saliva cortisol was measured before and after transportation. Treatment did not affect blood chemistries, gait, fecal biomarkers, or saliva cortisol (p ≥ 0.19). Eosinophils increased slightly in Controls (p = 0.01), though remained below 0.80 × 109 cells/L. Most cytokines were unaffected by treatment (p ≥ 0.15), but there were minor changes in circulating monocyte chemoattractant protein-1 (p = 0.01) and IL-8 over time at the initial run (p = 0.03) and IL-10 in males (p = 0.02) in the Low dose dogs. The High dose decreased Blautia (p = 0.04) slightly and tended to decrease Fusobacterium abundances (p = 0.07). The Low dose tended to increase Clostridium hiranonis (p = 0.07) slightly. The tested S. cerevisiae postbiotic produced small changes in immune function and gut microbial species in dogs.
]]>Authors: Marianne Pan Todd Archer Andrew Mackin Robert Wills Lakshmi Narayanan Jana Raessler Stacie Summers
The administration of modified cyclosporine with food has been reported to decrease oral bioavailability. The objective of this study was to compare the pharmacodynamic effects of oral modified cyclosporine in healthy dogs when administered with and without food. In this randomized, crossover study, eight client-owned dogs received a commercial formulation of modified cyclosporine (median dose, 3.5 mg/kg PO q12h) with food (fed phase) or one hour before food (fasted phase) for 7 days. Two hour post capsule administration was used as the expected time of peak cyclosporine concentration. Peak cyclosporine blood concentration measured by antibody-conjugated magnetic immunoassay and interleukin-2 (IL-2) mRNA expression assessed by quantitative reverse transcription PCR (qRT-PCR) were measured at baseline, seventh day of each phase, and after a 21-day washout. Three dogs were withdrawn due to refractory anorexia. For the five dogs that completed this study, no difference in cytokine expression (p = 0.9) was found when comparing qRT-PCR values between the fed and fasted phase. All dogs achieved post-treatment IL-2 expression corresponding with “moderate” levels of immunosuppression or higher after both phases. No difference in peak blood cyclosporine concentration was found between the 7th day of the fed phase (median, 2042; range, 1484–2100 ng/mL) and fasted phase (median, 1990; range 1137–2100 ng/mL; p = 0.8). Feeding dogs at the time of the administration of oral modified cyclosporine can achieve similar suppression of IL-2 expression and blood concentrations two hours post drug administration as compared to when the drug is administered without food.
]]>Authors: Sarah Gradidge Steve Loughnan Nic Gibson
Although many people strongly believe in wellbeing benefits of having pets (the ‘pet effect’), research on the ‘pet effect’ in actuality is highly mixed. Surprisingly, little research has explored the nature of (i.e., specific components of) the human–pet relationship, as a way to explain the contradictory findings. One such component is human–pet play, with play outside of the human–pet relationship being important for wellbeing and social buffering. Thus, the current quantitative study explores whether greater perceived play with pets contributes to greater wellbeing (‘pet effect’) and reduces anxiety during a time of acute stress (social buffering). The study employs a regression design recruiting men and women residing in the UK during the COVID-19 pandemic who own a dog and/or cat, with perceived pet play as the key predictor variable, and outcome variables of wellbeing and COVID-19 anxiety. Data were gathered and analysed cross-sectionally on day one (N = 189), and longitudinally over five days (N = 105), using multiple regressions. Overall, perceived pet play did not predict wellbeing nor COVID-19 anxiety. As such, the current study indicates human–pet play does not contribute to the ‘pet effect’ nor social buffering, thus raising questions for future research regarding the exact purpose of play within the human–pet relationship.
]]>Authors: Manon Toutain Marine Malivoir Pauline Brugaillères Inès Tiercelin Carole Jacq Yentl Gautier Camille Cagnot Albane Péchard Ronan Jubin Laurence Henry Martine Hausberger Marine Grandgeorge
Interacting with animals often provides numerous benefits for children with autism spectrum disorder (ASD). One potential explanation for this is that children with ASD exhibit particular visual attention to animals. This study aimed to characterize the visual attention patterns towards humans and animals in children with ASD compared to neurotypical (NT) children during one-time first exposure to an assistance dog. Forty children participated (18 ASD, 22 NT). The interventions were videorecorded. Three categories of visual attention (gaze, glance, and mutual gaze) directed towards assistance dogs and human professionals were coded using ethological methods. Both the children with ASD and the NT children gazed more frequently at the assistance dog than at the professional. However, although infrequently, both groups shared more mutual gazes with the professional than with the assistance dog. Both the NT children and the children with ASD preferred to gaze rather than to glance at, or share mutual gazes with, the assistance dogs. The attention patterns of the NT children towards the professionals were consistent, whereas those of the children with ASD were not. Statistical linear models revealed that a child’s status (ASD/NT) was the only factor influencing the frequency and duration of the visual behaviors observed. These results confirm that individuals with ASD exhibit particularly focused attention towards animals.
]]>Authors: Isabelle Kappel Marie-Christin Riedel Fiona Becker Shay Hicks Nicole Warlich-Zach Udo Ganslosser
The existing domestic cat literature predominantly contains lists detailing its behaviour. However, these lists are neither specific enough for domestic cats nor general enough for all behaviours on a macro level of the domestic cat. Furthermore, the majority of studies lack illustrations. Therefore, we have developed a comprehensive ethogram of the domestic cat and scalable schematic representations of the behaviour units. We evaluated observational data from free-roaming cats and cats from private households using ad libitum and focal animal sampling (video material 55.31 h, n = 170 animals). In addition, we used the leading literature and detailed anatomical studies to create systematic categories and morphologically correct drawings. A total of 117 discrete behaviours were recorded and divided into 12 categories. The description of each behaviour includes a name, a word definition, an ethological classification and a schematic illustration (excluding the category devoted to vocalisations). An ethogram is needed to better understand the behavioural repertoire of healthy domestic cats and to facilitate the design phase of further investigations. This document can help to better distinguish species-appropriate behaviours and body postures from those behaviours and poses that indicate chronic pain, stress or discomfort.
]]>Authors: Leena Awawdeh Rachel H. Forrest
Aims: To investigate the road safety concerns associated with pet dogs in New Zealand. Methods: An online survey was developed and offered to New Zealanders via SurveyMonkey© from 8 January 2019 to 31 March 2019) using New Zealand residents aged ≥ 18 years. Questions about the demographics of respondents and their number of pet dogs along with those about dog transportation and dog roaming were asked. Results: Of 2744 respondents who completed the online survey, 1494 (65%) owned a dog. Of the total respondents, 1511 completed the question about how their dog or dogs travel in vehicles. Of these, 2% (n = 29) let their dog sit on the driver’s knee, and 12% (n = 179) allowed their dog to sit on the passenger’s knee. Only 7% (n = 105) allowed the dog to roam free throughout the vehicle, while 44% (n = 663) allowed their dogs to roam free in the backseat area only and 19% (n = 280) put their dog in the boot/hatchback area. A minority of respondents (2%, n = 22) indicated they let their dog travel unrestrained on the deck of a ute or truck. Approximately half, 51% (n = 767), of the respondents indicated that they restrained or crated their dogs in or on the vehicle. Respondents also expressed concerns about roaming dogs being a road safety hazard with the themes of responsible ownership, physical and psychological harm, and the dangers of rescue altruism emerging. Conclusions: Increased awareness about proper pet restraints during transportation and preventing dogs from roaming, especially near and on roads, is crucial to ensure traffic safety for humans and dogs.
]]>Authors: Fredrik Hoholm Karin Bruining-Staal Paul J.J. Mandigers
Background: Chiari-like malformation (CM), Syringomyelia (SM) and middle ear effusion (MEE) are frequently observed disorders in Cavalier King Charles Spaniels (CKCSs), Pomeranians, and less frequently the Griffon. There are a few reports in which small dogs have been identified as suffering from CM/SM. Methods: From all MRI centers based in the Netherlands, MRI scans for small dog breeds performed for various reasons over a period of 8 years were collected. Scans that enabled a CM and/or SM evaluation were included and evaluated. Results: In total, 177 MRI scans were included. CM could be evaluated in 163 out of 177 dogs. In 63 dogs, no CM was observed, while 100 dogs had CM. SM could be evaluated in 144 out of 177 dogs. No SM was seen in 56 dogs and SM was seen in 88 dogs. Both CM and SM were seen in high frequency in the Chihuahua, French Bulldog, Griffon, and Pug but did also occur in lower frequencies in various other small breed dogs and crosses. CM and/or SM does occur in various other small dog breeds and crosses suggesting that it is indeed a type-related disorders. As it can cause serious clinical signs breeders should be aware of the risk of CM/SM when breeding with these small dog breeds.
]]>Authors: Sofia Zapata Isain Zapata
The AKC functions as the largest and most influential dog breed registry and breed standard information source in the United States. AKC breed standards describe the breed’s overall appearance and behavior. Despite their popularity, the AKC behavioral descriptions and the traits used to quantify them have largely remained unexplored. In this study, we used eleven behavioral breed standard traits for 286 breeds collected for the official AKC source. Dog size and appearance were also evaluated. We employed pairwise correlation and factor analysis to define behavioral trait constructs and hierarchical clustering methods to evaluate the similarity of breed profiles. These methods revealed that although AKC data exhibit various individual correlations concurrent with known canine behavior, many breeds present behavioral trait constructs that do not align with the narratives presented in their standard and may be lacking crucial factors in dog behavior, as suggested by breed group clustering. Our dimensional analysis revealed three constructs, which we labeled as “Social Engagement”, “Pet Demands”, and “Vigilance”, that are very important and relevant to pet ownership. These results emphasize the importance of standardized observations when quantifying behavior and provide an overarching context regarding the strengths and weaknesses of these descriptions, allowing readers to better understand and predict their pet’s behavior.
]]>Authors: Domingo Ruiz-Cano Marino B. Arnao
The use of nutraceuticals, mainly phytogenics, is increasingly widespread in animal nutrition, especially in dogs. The materials typically used to provide these very diverse natural compounds come from plants, but lately algae and fungi have also been used. In animal nutrition, these compounds are applied to obtain better results in the production and stability of feed and also as biofunctional substances with benefits for animal health. Polyphenols are natural compounds from the secondary metabolism of plant matter present in animal food (e.g., seeds and nuts, fruits, vegetables, herbs/aromatic plants, spices, cereals, and vegetable oils, among others). Most of the biological effects of these compounds associated with health benefits have been attributed to their antioxidant potential because they can protect cellular elements against oxidative injury, reducing the risk of dysfunctions and diseases associated with oxidative processes. Polyphenols are constituted by multiple families of substances with wide applications in pet therapy and nutrition. In this work, we review the most relevant phytogenic polyphenols, exploring their characteristics, sources, and implications for canine health. Our focus includes the effects on gastrointestinal functions and its microbiota, as well as aspects such as obesity, diabetes, and fat metabolism. Additionally, we examine their impact on cardiovascular, neurological, and immunological systems, along with their potential anti-oncogenic role. Finally, we discuss the overall role of polyphenols in dog diets and their future implications.
]]>Authors: John C. Rowe Emmy Luo Martha G. Cline Michael Astor John P. Loftus
Aminoaciduric canine hypoaminoacidemic hepatopathy syndrome (ACHES) is a rare syndrome affecting dogs. Nutritional management is a pillar of optimal treatment. Currently, there are no specific published data to inform dietary nutrient composition selections for treating affected dogs. Thus, our goal was to establish nutrient targets for the dietary management of ACHES by comparing nutrient profiles of commercial and home-cooked diets fed to dogs after diagnosis and determine if different nutrient inclusions were associated with survival. This retrospective cohort study evaluated nutrient profiles of commercial diets (n = 10) and home-cooked diets (n = 8) fed to dogs with ACHES. Associations between dietary nutrient inclusions and survival duration were determined using Cox proportional hazard analysis. Home-cooked diets were significantly (p < 0.001) higher in dietary protein and several amino acids than commercial diets. Risks of death were significantly (p < 0.05) lower with increasing dietary protein (hazard ratio 0.92 [CI 0.82–1.0]) and sodium (hazard ratio 0.02 [CI < 0.01–0.38]) and higher for dietary fat (hazard ratios 1.15 [CI 1.02–1.37]). An increased risk of death with increasing dietary calcium did not achieve significance (p = 0.067, hazard ratio 9.92 [CI 1.02–201.0]). These results were used to recommend target dietary nutrient ranges, in g/1000 kcal, of 90–130 for protein, 20–40 for fat, 0.7–1.8 for sodium, and 1.0–2.8 for calcium for dietary ACHES management.
]]>Authors: Zofia Gorzelanna Marta Miszczak
Microbiome research has become significantly advanced in recent years, both in human and veterinary medicine. The development of DNA sequencing technology has enabled a deeper understanding of the diversity of microorganisms inhabiting animal bodies. It has given clinicians, scientists, and behaviorists a chance of better understanding the impact that a proper microbial population has on the individual, enabling a much more holistic view of the animals’ health and welfare. Current knowledge is increasingly based on analyses of the impact of microorganisms present in the gastrointestinal tract on the neurobiology and behavior of the host. This review presents research results, indicating potential implications for fields such as ethology and veterinary medicine. Understanding the role of the microbiome in shaping animal behavior could open up new therapeutic opportunities and lead to more sustainable animal husbandry practices.
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