Microplastics doi: 10.3390/microplastics5030145

Authors: Simon Baniya Tusher Mohanta Moayad Yacoub Utsav Darlami Aihua Li Ishan Subedi Kirsten Nicholson Subodh Sharma Bangshuai Han

Microplastic contamination is an emerging global concern, but its occurrence in high-altitude protected areas has been understudied. This study systematically assessed microplastic abundance, morphology, and spatial distribution in Sagarmatha National Park (SNP), Nepal, a UNESCO World Heritage Site. Soil samples were collected from 25 sites across four land-use types, including settlement, farmland, forest, and floodplain, at two depths along the Lukla–Phortse trekking corridor during the pre-monsoon season of 2023. Samples were pretreated by density separation and Fenton’s reagent digestion, and microplastics were then detected using a YOLOv11n-seg instance segmentation model. A subset of extracted particles was chemically confirmed by optical photothermal infrared (O-PTIR) spectroscopy. Microplastics were present in all samples, with concentrations ranging from 80 to 960 particles·kg−1. Fragments were the dominant morphological type, accounting for 65.7% of all particles, followed by fibers and films. Negative binomial regression revealed significant effects of land use and soil depth and their interaction on microplastic abundance. Settlement soils showed the highest concentrations with significant surface enrichment, while farmland soils showed no significant depth effect, consistent with human-dominated plastic sources and tillage-driven redistribution. Elevation was not a significant predictor of contamination. Hotspot analysis identified statistically significant clustering around Lukla and Namche, the two primary tourism hubs. The baseline established in this study provides a foundation for long-term monitoring and targeted waste management in SNP and offers insights to other protected high-altitude environments. Meanwhile, the application of deep learning and O-PTIR to balance the counting efficiency and accuracy is a novel approach that could be adopted by other researchers.

Microplastics doi: 10.3390/microplastics5030143

Authors: Dhouha Belhaj Sghaier Noureddine Zaaboub Ines Chniti Thouraya Barhoumi-Slimi Micha J. A. Rijkenberg Monia El Bour

The pollution caused by microplastics (MPs) in marine sediments has been a concern of many researchers thanks to these substances’ persistence and potential ecological effects. To our knowledge, this study represents the first comprehensive investigation of MP contamination in marine sediments collected from multiple regions of the Mediterranean Sea, encompassing a wide bathymetric range from coastal areas to deep-sea environments, down to approximately 4000 m water depth. Sediment samples were taken from several sites in the Mediterranean and the northern Aegean Sea. Thanks to microscopic analysis, fragments and filaments were the dominant MP forms; their color and size varied, and the most frequent particle colors were red, transparent and blue. FTIR spectroscopy revealed diverse types of polymers, including polystyrene (PS), propylene (PP), ethylene–vinylacetate (EVA), polyamide (PA), and Acrylonitrile Butadiene Styrene (ABS) polymer. The 1-H NMR analyses confirmed the presence of PA, EVA, PP, polyethylene (PE), and PS, thereby supporting the FTIR results. This spatial variability in polymer composition probably reflects both regional anthropogenic inputs and hydrodynamic factors affecting sedimentary deposits. These results detail the complex and generalized nature of MP contamination in Mediterranean sediments and offer a basis from which to start assessing ecological risks and developing targeted mitigation strategies.

Microplastics doi: 10.3390/microplastics5030144

Authors: Elizabeth S. Gordon Maeve H. Ahern Nicole E. Burdick Kaitlyn E. Prentiss Paulina Torres Daniel P. Welsh

Microplastic pollution is an increasingly important environmental concern, especially in aquatic ecosystems. However, research on microplastics has historically been skewed towards marine systems and large freshwater systems, leading to an incomplete picture of the potential prevalence and scope of this type of pollution. In order to understand the potentially ubiquitous nature of freshwater microplastics, our study focused on 11 small headwater streams in north–central Massachusetts (USA) and a small freshwater fish species, the Blacknose dace (Rhinichthys atratulus). Microplastics were found in all sampled streams. Considerable variation in the number of stream microplastics existed, with concentrations ranging from 0.3 to 45.1 pieces per cubic meter of water. Microplastics were assessed in 14–20 fish from each of five of these streams. The vast majority of all fish had microplastics, with some individuals having over 200 pieces in their digestive tract. The number of fish microplastics was not found to be statistically related to the amount of water microplastics, the length of the fish, or the land use surrounding the streams. Together, these results illustrate that microplastics are prevalent and that small freshwater ecosystems need to be considered when assessing the full impact this source of pollution may have on ecosystems.

Microplastics doi: 10.3390/microplastics5030142

Authors: Mahrosh Javed Galina Lujanienė Sergej Šemčuk Tayyab Tahir Aušra Selskienė Vidas Pakštas Audrius Drabavičius Martynas Talaikis

With high mobility, long-term durability, and potential risks to aquatic ecosystems and human health, polyethylene nanoplastics (PE–NPs) are a developing environmental issue. Therefore, graphene oxide (GO) was utilized to functionalize chitosan (CS) and microcrystalline cellulose (MCC) in order to create sustainable polysaccharide-based composites that resulted in graphene oxide–chitosan (GO–CS), graphene oxide–MCC–50 µm and graphene oxide–MCC–90 µm adsorbents in order to evaluate the adsorption performance of sustainable GO–polysaccharide composites toward laboratory-prepared polyethylene nanoplastics under controlled conditions. The novelty of this study lies in the comparative evaluation of GO–chitosan and GO–microcrystalline cellulose composites with two MCC particle sizes (50 μm and 90 μm) for the adsorption of laboratory-prepared polyethylene nanoplastics, providing insight into the influence of adsorbent composition and particle size on adsorption performance. The prepared adsorbents were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), point of zero charge (pHpzc), and X-ray diffraction (XRD) in order to analyze adsorption behavior by morphology, chemical structure, surface chemistry, and particle crystallinity. According to the results, GO–CS has the maximum adsorption capacity (50.25 mg·g−1), followed by GO–MCC–50 µm (38.31 mg·g−1) and GO–MCC–90 µm (27.02 mg·g−1). Smaller MCC particle sizes of adsorbent resulted in improved adsorption performance, as demonstrated by the increased adsorption capacity of GO–MCC–50 µm as opposed to GO–MCC–90 µm. The difference between the graphene oxide–MCC–50 µm and graphene oxide–MCC–90 µm composites’ adsorption capacities demonstrates that adsorption capacity greatly increases with small adsorbent particle sizes. Stronger interaction sites, hydrophobic contacts, and a large number of functional groups are all responsible for the improved performance. The findings provide insight into the influence of polymer type and particle size on polyethylene nanoplastic adsorption and support the further development of GO-based biopolymer adsorbents.

Microplastics doi: 10.3390/microplastics5030141

Authors: Carol Adrianne Smith Saroj Pramanik

Anoplophora glabripennis (ALB) is an invasive wood-boring cerambycid that causes extensive damage to hardwood host trees through sequential tissue penetration from the bark to the sapwood. Developmental biology of ALB is well established. However, interactions among its life cycle, environmental contaminants, and fungal associates remain poorly understood. In particular, the ecological relationships among microplastics, entomopathogenic fungi, and the fungal symbiont Fusarium solani (FSSC) within ALB-associated woody tissues remain largely uncharacterized. This review develops a conceptual anatomical framework integrating ALB developmental biology, fungal associations, frass deposition pathways, and potential microplastic interactions within woody host tissues. The framework was constructed through ecological literature synthesis and anatomical reconstruction. To our knowledge, this represents the first conceptual framework integrating ALB developmental anatomy, fungal symbiosis, and potential microplastic interactions within host tree gallery systems. A longitudinal cross-sectional model was developed to illustrate oviposition, larval gallery formation, pupation, and adult emergence in relation to the outer bark, cambium/phloem, sapwood, and heartwood. FSSC isolates previously documented on ALB egg surfaces following oviposition and within ALB frass were examined, thereby positioning the fungal symbiont both within and outside galleries produced by ALB throughout its life cycle. Previous studies have demonstrated that microplastics can be taken up by plant stem tissues and accumulate on the forest floor through atmospheric deposition. This widespread presence suggests that micro- and nanoplastics may penetrate sapwood and heartwood galleries through xylem and phloem flow. These transport pathways may overlap with regions where late-instar larvae actively forage. The integrative framework presented here highlights potential ecological interactions within the gallery microhabitat and provides a foundation for future experimental investigations into contaminant–pathogen–host dynamics in xylophagous insects. While we refrain from proposing specific management strategies, we present a conceptual framework to elucidate how microplastics may serve as incidental contact points for cerambycid anatomy and fungal propagules. We hypothesize that these interactions link microplastic pollution to invertebrate ecology. Microplastics may function as substrates for fungal spores within forest canopies and gallery systems, potentially influencing fungal persistence, contaminant transport, and ecological dynamics within infested forest habitats.

Microplastics doi: 10.3390/microplastics5030140

Authors: Pratyusha Nayak Nishigandha Muduli Smruti Prajna Pradhan Subhashree Nayak Sthitaprajna Nath Sharma Lipika Patnaik

Microplastic (MP) pollution in marine ecosystems is a matter of concern worldwide, as their ingestion and accumulation in marine fauna pose a significant health risk associated with MP pollution. This detailed study is the first of its kind in the Chandipur coastal waters, Bay of Bengal to comprehensively evaluate MP pollution, and associated pollution load in commonly consumed edible fish and shrimp species. Samples of Harpadon sp., Setipinna taty, Coilia dussumieri, Johnius belangerii, Pampus sp., Arius arius, and Penaeus monodon were collected from Chandipur, Odisha, East coast of India and examined using wet peroxide oxidation, followed by FTIR spectroscopy. A relatively higher concentration of MPs was observed in the muscle tissues (4628 ± 11,702 particles/g of tissue) compared to the gastrointestinal tract (GIT) (4296 ± 9687 particles/g of tissue). The abundance of MPs was maximum in Coilia dussumieri and minimum in Arius arius. Comparatively higher retention of MPs (11,309 ± 15,448 particles/g of tissue) was obtained in the monsoon season than in the pre- and post-monsoon. Microscopic examination revealed the presence of mostly fiber- and fragment-type MPs, in addition to black fibers. The commonly encountered size class was 1–50 µm with 63.4%. FTIR analysis revealed eighteen different types of MPs and polyvinyl chloride (PVC), polyethylene terephthalate (PET), and cellulose acetate (CA) with occurrence percentage between 91.6 and 100%, whereas polyamide (PA) was the most uncommon and least found in all the samples. The Polymer Hazard Index (PHI) revealed the presence of extremely hazardous polymers such as polyurethane (PU), PVC, and PET in tissues, and Pollution Load Index (PLI) values indicate low pollution levels.

Microplastics doi: 10.3390/microplastics5030139

Authors: Varsha Deepali Chandra Rajnandini Verma Niharika Ajey Singh Pradeep Kumar

Plastic pollution has emerged as a major environmental concern due to its persistence and widespread accumulation in terrestrial ecosystems. The extensive utilization of plastics across a diverse range of products, from packaging to healthcare, construction, and transportation, poses a significant risk due to their enduring and non-biodegradable nature. Micro/nanoplastics (MNPs) derived either from the fragmentation of larger plastics or direct release are increasingly detected in agricultural soils, where they interact with plant systems. In addition, chronic exposure of MNPs alters soil structure, microbial diversity, and nutrient cycling, further impacting agroecosystem functioning. Plants have been shown to absorb MNPs mostly from contaminated soil and irrigated water through their root systems, allowing their subsequent translocation to aerial tissues. MNPs can enter plants through the aquaporins, apoplast pathways, crack entry modes, and leaf stomata, disrupting nutrient uptake, photosynthesis, and growth processes, ultimately affecting crop productivity and quality, while their accumulation in edible tissues raises concerns regarding food safety and trophic transfer. To address these challenges, it is crucial to have standard detection methods for identifying MNPs and to bridge the gap for further mitigation. This review further discussed effective mitigation strategies, including nanomaterial and phytohormone-based interventions under increasing plastic contamination.

Microplastics doi: 10.3390/microplastics5030138

Authors: Mina Popović Nevenka Rajić

The global proliferation of microplastics demands sustainable remediation alternatives to energy-intensive conventional disposal methods, shifting research focus toward polymer-degrading microbial communities within the “plastisphere.” The primary objectives of this study are twofold: first, to systematically decode the sequential biophysical mechanisms underlying microplastic colonization and enzymatic degradation; and second, to establish an empirically validated, scalable treatment framework that employs both a novel biological isolate and a hybrid engineering architecture. Experimentally, we investigate the multi-stage colonization process and demonstrate that “Phase Zero” conditioning films modulate the surface zeta potential (ζ) to anchor pioneer r-strategists. To evaluate degradative efficacy under accelerated conditions without abiotic pretreatment, the newly isolated carp gut strain Hafnia paralvei UUNT_MP29 was exposed to pristine low-density polyethylene (LDPE) and polystyrene (PS). Over a 16-day biotic incubation period, structural and chemical alterations were distinctly polymer-specific: bacterial action on the polyolefin LDPE yielded a Carbonyl Index of 0.4594 and a 10.95 °C reduction in thermal stability (Tmax), whereas the aromatic PS matrix exhibited a Carbonyl Index of 0.3235 alongside a 10.80 °C decrease in Tmax, with both substrates showing intense surface pitting. To standardize these complex tracking metrics across the field, a universal four-pillar Biodegradability Index (BI) was formulated. Based on these findings, we recommend an immediate transition from passive waste containment to a closed-loop engineering approach. Specifically, we propose integrating an artificial intelligence (AI)-managed hybrid bioprocess configuration that couples Advanced Oxidation Processes (AOPs) with Membrane Bioreactors (MBRs). This dual-stage configuration is recommended to overcome polyolefin crystallinity, accelerate stoichiometric mineralization, and actively mitigate additive-mediated toxicity at the industrial scale, providing a vital blueprint for the circular bio-economy.

Microplastics doi: 10.3390/microplastics5030137

Authors: Lorenzo A. Picos-Corrales Anette López-Guardado Ana M. Morales-Burgos Alfonso Talavera-Lopez Jose Alfredo Hernández Oscar Joaquín Solís-Marcíal Levy N. Inzunza-Camacho Jose P. Ruelas-Leyva

Concerns have increased significantly in recent years due to the presence of microplastics in different environmental compartments given that this pollutant can cause adverse effects on the environment and human health. The present review integrates representative studies of Mexican researchers proposing solutions to these concerns, addressing ecological risk and the human food chain, microplastic ingestion by animals, water and sediment pollution, physical/chemical methods for microplastic removal from water, and chemical recycling as a research direction in plastic waste management. Several publications from Mexican institutions are limited to the occurrence and identification of polymers, and a smaller number of documents are focused on solutions to microplastic pollution. Fibers, fragments, spheres, films, and foams have been found in aquatic compartments, sediment, and animals. High ecological risk has been documented in some aquatic compartments. There is a lack of standardized protocols for sampling, extraction, identification, and reporting. Flocculation is a cost-effective approach and may be one of the most promising options for removing microplastics from fresh water. Bioremediation using microorganisms and chemical recycling appear to be the two most widely considered approaches to reverse plastic pollution. National databases, permissible limits, and mandatory monitoring programs should be developed, as these are essential components of an effective regulatory framework.

Microplastics doi: 10.3390/microplastics5030136

Authors: Nguyen Truong Thanh Pham Van Toan Huynh Vuong Thu Minh Kim Lavane Nguyen Vo Chau Ngan Le Thi Kim Ngan Vo Thanh Toan Nguyen Van Tuyen Pankaj Kumar

Microplastic pollution in tropical urban rivers has become an increasing environmental concern due to rapid urbanization, inadequate waste management, and hydrological transport processes. This study investigated the occurrence, characteristics, and spatiotemporal distribution of microplastics in the Can Tho River, Vietnam, along an urban–peri-urban–rural gradient during dry and wet seasons. Surface-water samples were collected at 15 sites and analyzed for microplastic abundance, density, shape, color, and size composition using stereomicroscopic identification and statistical analyses. Microplastics were detected at all sampling sites in both seasons, indicating widespread contamination throughout the river system. Although seasonal differences in overall abundance and density were not statistically significant at the basin scale, clear spatial variability was observed, particularly in urban and peri-urban regions. Fibers and fragments were the dominant shapes, while blue, purple, and green particles were the most common color categories. Particles larger than 1000 µm accounted for the largest proportion of detected microplastics, and continuous size-distribution analysis revealed broadly similar overall distributions, although a greater proportion of smaller particles was observed during the dry season. The results suggest that hydrological conditions, urbanization, and land-use characteristics may contribute to the observed spatial and seasonal patterns of microplastic distribution in the Can Tho River. Peri-urban zones exhibited the greatest seasonal variability, highlighting their role as transitional areas that may influence microplastic redistribution in tropical river systems. This study provides baseline information for understanding microplastic pollution in the Mekong Delta and supports future river management strategies.

Microplastics doi: 10.3390/microplastics5030135

Authors: Joaquin Hernandez-Fernandez Michel Murillo Acosta

Polypropylene (PP) waste, including microplastic debris, motivates molecular-scale studies of the intrinsic factors governing thermal degradation. In this work, the bond dissociation energies (BDEs) of C–C and C–H bonds were systematically evaluated in a finite isotactic polypropylene oligomer containing fifteen propylene repeat units, (–C3H6–)15, using Density Functional Theory at the M06-2X/LANL2DZ level. Thermochemical corrections were evaluated at 873.15 K, a temperature relevant to pyrolysis studies. Within the selected oligomer model, C–C bonds exhibited lower BDE values (82.28–87.41 kcal·mol−1) than C–H bonds (90.18–104.93 kcal·mol−1), indicating a thermochemical preference for backbone scission. The lowest calculated BDE values were associated with specific tertiary carbon environments, including sites C24 and C28. A mixed-effects model identified bond type and carbon type as the principal factors associated with BDE variation, while principal component analysis summarized the covariation among the electronic and thermodynamic descriptors. These results provide a molecular-scale description of intrinsic scission tendencies within the selected PP oligomer and establish a basis for subsequent kinetic, catalytic, and experimental studies.

Microplastics doi: 10.3390/microplastics5030134

Authors: Francisco Alvirde-Díaz Fredy Cuellar-Robles Javier Illescas Alethia Vázquez-Morillas María del Carmen Carreño de León María del Consuelo Hernández-Berriel

Microplastics (MP) in landfill leachate represent an analytical challenge due to matrix complexity and the need for methods that remove interferents without degrading polymers. This study evaluated the efficiency of four digestion methods (30% H2O2, Fenton, 10% NaOH, and 20% HCl) and three density separation solutions (CaCl2, NaI, and ZnCl2) for MP quantification in leachate from the Zinacantepec Sanitary Landfill, Mexico. Samples were spiked with seven polymer types (polyethylene terephthalate (PET), high-density polyethylene (HDPE), polyvinyl chloride (PVC), low-density polyethylene (LDPE), polypropylene (PP), polystyrene (PS), and polyamide (PA)). Results analyzed by ANOVA (p < 0.05) showed that Fenton reagent was the most efficient digestion method, achieving 99% MP recovery, whereas alkaline and acid digestions caused degradation of PET, PS, and PA. Regarding density separation, ZnCl2 (1.7 g/cm3) achieved recovery exceeding 99% for all polymers. The proposed protocol enables effective isolation and identification of degraded microplastics, contributing to advance the understanding of degradation processes and transformation pathways of MP in complex environmental matrices. The combination of Fenton digestion and ZnCl2 separation showed the highest overall performance, with an efficiency greater than 96%, supporting its use as a reliable protocol for MP quantification in leachate and contributing to methodological standardization in this field.

Microplastics doi: 10.3390/microplastics5030133

Authors: Juzhuang Wang Shengwang Yu

This study systematically investigated microplastics (MPs) in Pingshan River, Shenzhen—a representative urban river with short channel length, rapid flow, and limited environmental capacity. Surface water and sediment samples from seven sites were analyzed for MP abundance, size, morphology, color, and polymer composition. Results revealed significant MP pollution: surface water abundance ranged from 132 to 423 items/L (mean 311.42 ± 90.78 items/L), while sediment abundance ranged from 334 to 756 items/kg (mean 508.85 ± 151.79 items/kg). Spatial heterogeneity was pronounced, with the highest abundance at a construction-influenced site (Site 6) and the lowest at a less-impacted site (Site 2). MPs were predominantly 300–1000 μm in size. Fibers dominated surface water, while fragments prevailed in sediment. Transparent particles constituted >77% of all MPs. Polymer composition was dominated by polypropylene (PP) and polyethylene (PE). Key factors controlling spatial distribution included proximity to construction/industrial activities, aquatic vegetation cover, and hydrological conditions during the dry season. Polymer hazard risk index (H) classified all sites as Category II (10 ≤ H < 100), indicating low ecological risk despite high abundances. This research provides a scientific foundation for targeted pollution control in urban river systems, emphasizing the need to consider both abundance and polymer-specific toxicity in risk assessment.

Microplastics doi: 10.3390/microplastics5030132

Authors: Jhosin Jaik B. Pardillo Jay Rumen U. Maglupay Najiha B. Amer Rodolfo A. Romarate II Ruei-Feng Shiu Hernando P. Bacosa

Microplastic pollution is an emerging environmental concern in coastal ecosystems, particularly in developing regions. However, research remains compartmentalized, limiting an integrated understanding of microplastic distribution, transport dynamics, and ecological risks across interconnected environmental matrices. In this study, a multi-matrix assessment was conducted to evaluate microplastic abundance, characteristics, and associated ecological risks. A total of 93 microplastic particles were identified, with the mangrove site exhibiting the highest concentration (200 items/kg), while the seagrass bed and estuary showed the lowest concentration (3.33 items/kg). The dominant microplastic type was primarily fiber (55.91%), with most particles ranging from 0.1 to 1 mm, and polypropylene (66.67%) was the predominant polymer type, reflecting the widespread contribution from plastic packaging and fishing gear. Significant correlations were observed between microplastic abundance and contamination factor (CF), pollution load index (PLI), and potential ecological risk index (PERI), whereas the polymer hazard index (PHI) showed no significant relationship due to its dependence on polymer composition. Non-metric multidimensional scaling (NMDS) revealed distinct distribution patterns of microplastic shape and polymer type across matrices. Overall, microplastic distribution across environmental matrices is driven by heterogeneous sources and transport pathways, with mangrove sediments enhancing retention, underscoring the need to elucidate seaward and landward source contribution and coastal fluxes.

Microplastics doi: 10.3390/microplastics5030131

Authors: Ramesh Ganpisetti Sanjay Giridharan Mehmet Remzi Dokmeci Radhika Chandankere

The plastic pieces of synthetic polymers, which were previously regarded as primary pollutants of the environment, are increasingly being discovered as internal pollutants of the human body. This review provides a comprehensive overview of the available evidence on human exposure, tissue distribution, and associated biological effects of micro- and nanoplastics. Ingesting contaminated food and water is the major exposure pathway, with inhalation and dermal contact being secondary routes. Various organ systems have been identified as containing polymer particles through the use of advanced analytical methods, including blood, liver, lungs, placenta, breast milk, and brain tissue. Experimental animal studies suggest associations with tissue injury, metabolic illness, and neurotoxicity. Polyethylene, polypropylene, polystyrene, and polyethylene terephthalate are the most frequently found polymers in human samples. New clinical findings indicate potential health implications, though current human evidence remains largely associative rather than causal: a cardiovascular study observed more than a two-fold rise in mortality among patients with polymer-containing arterial plaques, and recent evidence demonstrates over-accumulation of polymers in brain tissue, raising questions about neuroinflammatory processes. Detection technologies have advanced substantially, with deep learning-based polymer classification achieving 95–99% accuracy and ultrasensitive electrochemical and surface plasmon resonance biosensors reaching detection limits approaching 10−11 M. Despite these advances, critical issues remain, including lack of standardized analytical procedures, absence of chronic exposure models for humans, and insufficient longitudinal epidemiological data. To address these gaps, physiologically relevant experimental systems including organoids and organ-on-chip platforms will be required, in addition to well-designed prospective cohort studies.

Microplastics doi: 10.3390/microplastics5020130

Authors: Florinela Pirvu Iuliana Paun Florentina Laura Chiriac

Microplastics (MPs; <5 mm) represent a growing environmental concern that increasingly challenges environmental monitoring, governance, and evidence-based decision-making. This review critically examines how current scientific understanding of microplastic sources, classification, occurrence, and environmental behavior can support environmental governance. MPs are classified as primary and secondary particles; however, persistent inconsistencies in size definitions, shape descriptors, and polymer identification limit the comparability of monitoring data and constrain the development of coherent regulatory frameworks. Evidence on the occurrence of MPs in surface waters and sediments highlights widespread contamination and pronounced spatial variability, raising challenges for risk assessment and policy harmonization across regions. Key transport pathways, including atmospheric deposition, terrestrial runoff, and riverine fluxes, are analyzed to illustrate how local emissions translate into large-scale environmental impacts. Rivers emerge as key components linking sources to receptors, offering relevant points for policy intervention and management measures. The review evaluates current policy responses to microplastic pollution, identifying significant gaps in standardized monitoring, data integration, and risk assessment approaches. It emphasizes the need for stronger alignment between scientific outputs and policy requirements, including the co-production of knowledge involving scientists, regulators, and stakeholders. By outlining pathways through which scientific evidence can inform regulatory design and environmental management, this study provides actionable insights for improving policy effectiveness. Advancing harmonized methodologies and integrating science into decision-making processes are essential steps toward mitigating microplastic pollution and supporting sustainable environmental governance.

Microplastics doi: 10.3390/microplastics5020129

Authors: Raffaelina Mercogliano Alessandro Avolio Stefano Capone Margherita Ferrante Gea Oliveri Conti Rossella Di Palo Maria Carmela Ferrante

The seasonal distribution of microplastics, as a representative case, was examined in Mytilus galloprovincialis from a pilot farm in the Gulf of Naples (Italy). The influence of marine parameters on microplastic uptake rate was assessed. A destructive patented method was used, and two microplastic size classes (<10 µm; >10 µm) were defined. Estimated Daily Intakes were calculated for different age groups. Results showed a significant abundance of small microplastics (9683.92 ± 6911 vs. 41.85 ± 13.98). In mussels, the highest levels (19,738.13 ± 3406.04) were detected in summer, and the lowest in autumn (4145.56 ± 2364.93). Summer variations in seawater temperature, oxygen, and pH were significantly different from those in winter and spring. High exposure levels, mainly of microplastics < 10 µm, were observed in the elderly (318.08 ± 227.00), followed by adults (225.29 ± 160.78) and children (212.29 ± 151.50), with the lowest in teenagers (127.51 ± 91.00). Despite the high variability of factors influencing mussel filtration and microplastic uptake, the study provided data on the seasonal microplastic distribution pattern and a size-based screening exposure level. Results highlight the importance of geographic and seasonal conditions, and particle size in assessing microplastic exposure through farmed mussel consumption.

Microplastics doi: 10.3390/microplastics5020128

Authors: Jana Rammal Assi Al Moussawi Chaden Haidar Mikhael Bechelany Dalia El Badan Ismail Hijazi Akram Hijazi

Microplastics (MPs) have emerged as persistent environmental contaminants due to their persistence, widespread distribution, and potential risks to the environment and human health. This review focuses on the sources of MPs, their potential environmental risks, and human impacts, as documented in the recent literature from 2020 to 2026. Recent studies focusing on pathways, environmental weathering, and toxicity were evaluated and synthesized into the analysis. Previous studies have demonstrated that microplastics are transported across and between environmental compartments. Environmental degradation, driven by ultraviolet radiation, mechanical fragmentation, and oxidation, can alter microplastics’ surface characteristics, which may affect microplastic mobility, reactivity, and the solid-state adsorption of contaminants. Human exposure occurs primarily through ingestion and inhalation, with dermal and occupational exposure also contributing under certain conditions. Emerging evidence from in vitro, animal, and human tissue studies suggests that smaller particles, particularly nanoplastics, may contribute to oxidative stress, inflammation, and cellular injury; however, important uncertainties remain regarding environmentally realistic exposure levels, long-term health outcomes, and the extrapolation of experimental findings to real-world human health risk. Overall, the current literature highlights the need for standardized methodologies, improved integration of environmental monitoring and exposure assessment, and stronger evidence to support risk assessment and policy development.

Microplastics doi: 10.3390/microplastics5020127

Authors: Joaquín Alejandro Hernández Fernández Juan Carrascal Jose Alfonso Prieto Palomo

The pyrolysis of polypropylene (PP) microplastics offers a potential route to convert plastic waste into fuel-range hydrocarbon mixtures and chemical feedstocks. However, the elementary radical pathways underlying the formation of medium-chain hydrocarbon fragments remain insufficiently resolved. In this study, a representative isotactic PP oligomer model (C45H92) was evaluated using a comparative density functional theory (DFT) framework. The main mechanistic analysis was based on M06-2X, ωB97X-D, and M11 calculations combined with the def2-TZVP basis set, whereas LANL2DZ was retained only as a lower-cost comparative level during reaction-pathway exploration. Thermochemical profiles were evaluated over a temperature range of 298–923 K. Three selected pathways involving mid-chain homolytic cleavage, intramolecular hydrogen transfer (backbiting), radical rearrangement, and β-scission were examined. Within the selected reaction set, Route 1 exhibited a comparatively more favorable thermochemical profile than Routes 2 and 3 and provided a mechanistically plausible sequence toward medium-chain hydrocarbon fragments. The −TΔS contribution strongly influenced the calculated Gibbs free-energy profiles because fragmentation increases the number of molecular species under the ideal-gas thermochemical approximation. Accordingly, the ΔG values were interpreted comparatively and were not treated as direct evidence of spontaneous fragmentation under condensed-phase pyrolysis conditions or as quantitative predictions of experimental product selectivity. Differences among the evaluated functionals further indicate that the relative description of radical intermediates and transition-state regions is method-dependent. These results provide a molecular-level framework for future studies integrating quantum-chemical calculations, microkinetic modeling, and experimental product characterization.

Microplastics doi: 10.3390/microplastics5020126

Authors: Melinda Haydee Kovacs Emoke Dalma Kovacs

Background: Soil mulching is a widely adopted agricultural practice known to regulate soil microclimate and enhance crop productivity; yet the biochemical mechanisms by which intact plastic and biodegradable mulch films influence soil nitrogen (N) cycling at the metabolic pathway level remain largely unexplored. Understanding these nitrogen transformation pathways is critical for assessing the long-term impacts of mulching materials on soil microbial communities, soil health, and sustainable agricultural management. This study focuses on the biochemical effects of intact mulch film application on soil N metabolism. Methods: N cycle-related soil metabolites were profiled using GC–MS/MS and MALDI TOF/TOF MS and then integrated with multivariate statistical modelling and pathway-level metabolic network perturbation analysis to compare conventional plastic and biodegradable plastic mulch film application against unmulched controls. Results: A panel of 62 KEGG-annotated N-cycle metabolites was profiled, and material-dependent metabolome separation was confirmed by OPLS-DA (R2Y 0.893–0.956; Q2 0.546–0.786). Both mulching materials significantly perturbed soil N-metabolite pools but differed in terms of pathway identity, magnitude, and directionality. Conventional plastic mulching caused the greatest disruption—near-complete suppression of N-storage and stress-adaptation pools (NES of −1.16; impact score of 10.01) and severe impairment of aspartate-centred metabolism—with L-aspartate identified as a critical stoichiometric hub. Biodegradable mulching material imposed a distinct profile dominated by inhibition of branched-chain amino acid catabolism and lysine degradation, with L-pipecolate as a treatment-specific critical impact node. Conclusions: These findings support that mulching material choice is a primary determinant of soil N-cycling biochemistry. The observed metabolite-level perturbations are suggestive of potential consequences for nitrogen retention. Though this inference is based on metabolite pool size differences and network topology metrics rather than directly measured process rates, it should therefore be interpreted with appropriate caution.

Microplastics doi: 10.3390/microplastics5020125

Authors: Sneha Siwach Padma Dolkar Aarzoo Yadav Apoorva Atri Meenu Chaurasia Pankaj Yadav Themchuirin L. Sonia Nongmaithem Vyakhya Singh Aviral Singh Ram Krishan Negi

Microplastic (MP) contamination is an emerging threat to aquatic ecosystems. However, species-specific bioaccumulation patterns across trophic guilds in tropical river ecosystems remain scarcely understood. This study assessed the occurrence, organ-level distribution, polymer composition, and ecological risk of MPs in 220 fish representing 12 species, spanning across multiple trophic guilds, sampled from four sites along a pollution gradient of the river Yamuna, India. MPs were detected in all examined species, confirming extensive distribution across the river ecosystem. A total 1678 MPs were recovered, with significantly higher abundance in fish from the highly urban Delhi stretch than in those from upstream regions (Kruskal–Wallis, H = 11.03, p = 0.011). The highest species-specific MP load was recorded in omnivorous Oreochromis niloticus from Sonia Vihar (436 MPs), whereas the carnivorous species Xenentodon cancila exhibited the lowest accumulation (37 MPs). Surface- and mid-water herbivores and omnivores accumulated more MPs than benthic carnivores and detritivores. Nonetheless, spatial pollution gradients exerted a stronger influence on MP accumulation, compared to trophic guilds. The gastrointestinal tract exhibited the highest MP abundance (751 MP particles), followed by gills (605) and muscle tissues (322), confirming ingestion as primary uptake route, and suggesting possible tissue translocation. Fibers dominated in the assemblage (77.8%), while transparent (44%) and blue (19.5%) were most abundant colors. ATR–FTIR analysis confirmed 10 diverse polymers, with polyethylene (≈24%) and polypropylene (≈21%) together accounting for nearly half of the identified particles. The Polymer Hazard Index analysis classified the recovered MP mix as Category IV (high ecological hazard). These findings identify the Delhi stretch of the Yamuna as a high MP contamination zone and highlight the combined influence of urban pollution and fish ecology on MP bioaccumulation.

Microplastics doi: 10.3390/microplastics5020124

Authors: Yusmila Helguera Pedraza Nathalie Bernard Ana Flavia Roldan Ramos Dariadelys Reyes Noa Joán I. Hernandez-Albernas Anamary Acosta Valladares Marco A. Garcia Varens Arianna García Chamero Marc Metian Lorena Rios Francois Oberhaensli Carlos Alonso-Hernandez

Microplastic contamination in coral reef environments is increasingly recognized as a global concern; however, the extent to which polymer composition can help distinguish contamination sources and transport-related processes remains poorly understood. In this study, we assessed the abundance, composition, and diversity of microplastics (20–300 µm) across multiple reef systems in the Cuban archipelago using high-resolution Laser Direct Infrared (LDIR) spectroscopic analysis. Microplastic abundance varied substantially among sites, with a median concentration of 66 particles L−1 (IQR: 45–115 particles L−1), ranging from 8 to 218 particles L−1. A total of 11 polymer types were identified, with polyethylene (PE), polypropylene (PP), and polyamide (PA) dominating the assemblages and accounting for approximately 77% of detected particles. While these polymers were consistently observed across all sites, suggesting a pervasive regional background signal, highly impacted reefs exhibited more heterogeneous polymer profiles, including increased contributions of polyurethane (PU), polytetrafluoroethylene (PTFE), and polyvinyl chloride (PVC), consistent with localized anthropogenic influence. Multivariate analysis revealed moderate compositional structuring among reef sites and suggested broad differences in polymer assemblages associated with contrasting contamination settings. Notably, some reefs exhibited elevated microplastic abundances while remaining dominated by common polymers, indicating a partial decoupling between contamination levels and polymer-specific signatures. This pattern is consistent with the influence of regional transport and mixing processes across the Caribbean basin, potentially including circulation associated with the Yucatán Channel, although hydrodynamic processes were not directly assessed in this study. Overall, the findings highlight the value of polymer-resolved analysis for improving interpretation of microplastic contamination patterns in coral reef environments. The integration of polymer composition with abundance and diversity metrics provides a useful framework for distinguishing between localized contamination signals and broader regional background influences. This study represents a regional baseline assessment of small microplastics in Caribbean coral reef systems using high-resolution spectroscopic characterization.

Microplastics doi: 10.3390/microplastics5020123

Authors: Angeliki Tsachouridou Dimitra Voutsa

Background/Objectives: Tire (or tyre) wear particles (TWPs), originating from road traffic, have been recognized as a significant emerging contaminant for terrestrial ecosystems. The aim of this study is to attempt a critical review of the existing articles, to identify trends and directions in research, highlight any knowledge gaps, limitations and drawbacks, and develop respective proposals and recommendations. Methods: A comprehensive literature search was conducted in the PubMed and Web of Science databases for the period 2020–2025 according to PRISMA-based protocols. Results: The final studies were methodically grouped into specific representative themes. Conclusions: This study focuses on the factors affecting the emissions of TWPs, their size distribution, processes that affect their environmental fate and methodological approaches for characterization/determination of TWPs. This article also explores the occurrence and toxicity of TWPs in the terrestrial environment, as well as the management approaches and policies in order to minimize their impact.

Microplastics doi: 10.3390/microplastics5020122

Authors: Erica Sparaventi Rafael Nuez María Pilar Yeste Miguel Ángel Cauqui Marta Sendra

Microplastics (MPs) are routinely detected throughout wastewater treatment plants (WWTPs), yet current treatment trains were not designed specifically to remove them. This study quantified and characterized visually identified MPs in influent and effluent waters from seven urban WWTPs in Andalusia (southern Spain) during a six-month monitoring period (July–December 2020). The targeted analytical size range was 45–5000 µm, and a subset of particles was further characterized by FTIR. MPs were detected in all sampling campaigns. Concentrations ranged from 6 to 78 items/L in influent and from 12 to 65 items/L in effluent. Fibers were the dominant morphology, and the 100–500 µm size class was the most represented fraction. Among the subset analyzed by FTIR, PA, PP, PVC and LDPE were the most frequent polymer assignments, with PA predominating in the fiber-rich fraction. However, because influent and effluent 24 h time-composite samples were not hydraulic retention time (HRT)-paired and FTIR interpretation was based on a selected subset of particles, the dataset is best interpreted as describing spatiotemporal variability during the study period rather than robust process-specific removal efficiency. Overall, the results support WWTPs as an ongoing pathway for MP release to receiving environments.

Microplastics doi: 10.3390/microplastics5020121

Authors: Sabela Fernandez-Sanchez Maria Garcia-Marti Jesus Simal-Gandara Juan C. Mejuto

In recent decades, plastic consumption has risen across various industries and everyday products, leading to greater plastic use and the generation of waste, which results in the leaching of micro- and nanoplastics into the environment. This review summarizes recent analytical methods for the detection of nanoplastics (NPs) in several marine matrices, divided into three main stages: extraction, separation, and identification. The literature reviewed indicates that chemical and enzymatic digestion are the most commonly used procedures for the extraction step. For the separation step, flotation, filtration, and centrifugation are the most used techniques. Finally, two groups of techniques may be used for the identification step. The first category consists of methods used for qualitative identification, with spectroscopic methods such as Raman and FTIR being the most frequently used. The second category comprises those used for the quantitative analysis of NPs, where fluorescence-based methods and nanoparticle tracking analysis are increasingly used for this assessment. Despite these advances, significant challenges remain, such as matrix interferences caused by salinity and organic matter, low environmental concentrations of NPs, and the lack of standardized protocols. This review highlights the need for standardized protocols, validated reference materials, and integrated multi-technique approaches to improve the comparability of nanoplastics measurements in marine environments.

Microplastics doi: 10.3390/microplastics5020120

Authors: Umair Sarfraz Shazia Alam Yinsen Qian Quan Ma Min Zhu Jinfeng Ding Chunyan Li Wenshan Guo Xinkai Zhu

The sustainability, crop production, and food safety of agriculture are increasingly challenged by microplastic pollution, as agricultural soils are the largest reservoirs and may serve as points of contact for plastic particles in the food chain. This review provides a comprehensive overview of plant materials, fate and uptake pathways, detection techniques, and the possible risks of microplastics in agriculture. Agroecosystems are also a source of microplastics, such as plastic mulch films, sewage sludge, compost and manure additives, wastewater irrigation, polymer-coated fertilizers, greenhouse materials, atmospheric deposition, and decomposition of discarded agricultural plastics. Their distribution and mobility in soil are controlled by polymer composition, particle size, morphology, density, surface ageing, soil texture, organic matter content, tillage practices, runoff, leaching, and soil biota. Recent data show that microplastics, especially smaller microplastics and nanoplastics, can attach to root surfaces, penetrate plants via cracks in roots, areas of lateral root development, and apoplastic pathways, and eventually move to tissues aboveground. Plant tissue detection is often accomplished by digestion of the sample, density separation, visual and fluorescence microscopy, Fourier-transform infrared spectroscopy, Raman spectroscopy, pyrolysis–gas chromatography mass spectrometry, and electron microscopy, but standardization of these methods remains a significant challenge. Microplastics can disrupt seed germination, root structure, nutrient absorption, photosynthesis, oxidative homeostasis, biomass buildup, yield development, and quality. Further, their capacity to transport additives, plasticizers, heavy metals, and persistent organic pollutants raises concerns about the transfer of contaminants to edible plant parts and their potential transfer to human diets. Further studies are needed focusing on field-realistic exposure conditions, long-term crop–soil interactions, nanoplastics behaviour, standardised analysis procedures, uptake and translocation pathways, edible crop risk assessments, and sustainable mitigation approaches to reduce microplastics in agroecosystems.

Microplastics doi: 10.3390/microplastics5020119

Authors: Jillian A. Verble Michael L. McKinney

Plastic pollution is an increasing concern in freshwater ecosystems, yet the roles of polymer chemistry, environmental context, and microbial community composition in governing degradation remain poorly resolved. This study examined plastic–microbe interactions across river, creek, and pond environments using gravimetric mass loss, scanning electron microscopy (SEM), and 16S rRNA gene sequencing. Four polymers were evaluated: biodegradable polyhydroxyalkanoate (PHA) and polylactic acid (PLA), and conventional low-density polyethylene (LDPE) and polyethylene terephthalate (PET). Rapid biofilm formation occurred on all plastic surfaces, indicating widespread microbial colonization; however, measurable degradation was strongly polymer-dependent. PHA exhibited rapid and extensive mass loss across environments, approaching complete degradation after four months in river and pond settings, whereas PLA, LDPE, and PET showed limited mass loss despite substantial colonization. Environmental context influenced degradation intensity, but these effects amplified degradation only when polymer chemistry permitted breakdown. Microbial community analyses showed that substrate presence influenced beta diversity more than alpha diversity, and differential abundance patterns revealed overlapping enriched taxa across polymers. Overall, degradation was governed primarily by polymer chemistry and environmental conditions, while microbial composition played a secondary, indirect role.

Microplastics doi: 10.3390/microplastics5020118

Authors: Aly Castillo Marta Lores Manuel Aira Jorge Domínguez

Applying sludge from wastewater treatment plants to agricultural soils is a major pathway for microplastics (MPs) to reach terrestrial ecosystems, with critical implications for food and environmental safety. A longitudinal analysis (13 months) was conducted to evaluate vermicomposting (with Eisenia andrei) as a remediation strategy, comparing fresh sludge, worm casts, mature vermicompost, and control (earthworm-free) compost. MPs were isolated by chemical digestion and density separation and characterized by optical microscopy and μ-Raman spectroscopy. The MP content of fresh casts (584 ± 45 MP·g−1; p = 0.036), driven by the mechanical and digestive activity of earthworms, showed a significant increase relative to sludge, in contrast to the invariant results observed in the control compost. The MP content of the vermicompost initially increased to 755 ± 88 MP·g−1 after 3 months of maturation due to gradual fragmentation by microbial degradation. However, after 13 months, the MP content in vermicompost, compared to the initial sludge, decreased by 62% (reduction of 625 ± 49 MP·g−1; p < 0.001), more than the 56% (reduction of 560 ± 83 MP·g−1; p = 0.001) observed in the control compost, suggesting a net long-term decrease. Morphological, colorimetric, and compositional changes, reflected by browning and reduced particle size and natural fiber content, revealed a temporal lag, with earlier transformation in vermicomposted samples. Overall, the findings show the potential of vermicomposting to reduce the MP content of sewage sludge used as a soil amendment.

Microplastics doi: 10.3390/microplastics5020117

Authors: Fidel Vallejo Diana Yánez Lorena Molina Ernesto Pino-Cortés Andrea Espinoza-Pérez Lorena Espinoza-Pérez

Microplastics constitute an emerging contaminant of major concern in Latin America, where human exposure predominantly occurs through ingestion of drinking water and marine/estuarine food chains. This review synthesises available evidence on occurrence, exposure pathways, toxicological mechanisms, and regional public health risks, while examining regulatory and monitoring limitations that constrain effective risk management. Reported concentrations in drinking water show a wide range (1–1194 particles/L), dominated by PET, PP, and PS, with fibres and fragments as the main morphotypes. In commercial marine species, prevalence reaches 70–100%, with burdens up to 44 particles/g in oysters and ~90 particles/250 g in mussels. Estimated Daily Intake is 2–5 times higher in children (e.g., Chile: 13.03 vs. 5.59 particles/day in adults). Toxicological mechanisms include oxidative stress, chronic inflammation (NF-κB pathway), endocrine disruption, intestinal dysbiosis, systemic translocation, and placental transfer, exacerbated by vectorization of local co-contaminants (Hg from mining, Cd/Pb from agriculture). Risk indices indicate extreme danger in Brazil, Chile, and Ecuador, where data are available. Significant geographic and methodological gaps persist, with Brazil dominating research (~50–60%). Multicenter biomonitoring, harmonised surveillance networks, and SDG-aligned policies are urgently needed to reduce exposure burdens, protect vulnerable populations, and advance toward comprehensive regional risk assessment.

Microplastics doi: 10.3390/microplastics5020116

Authors: Andros M. Po Rodolfo A. Romarate Cordulo P. Ascaño Christine Joy M. Pacilan Mei-Fang Chien Hernando P. Bacosa

Atmospheric microplastics are increasingly recognized as emerging contaminants in urban air, yet evidence from Philippine cities outside Metro Manila remains limited. This study provides a preliminary roadside baseline assessment of airborne microplastics in Cagayan de Oro City, southern Philippines. Atmospheric particles were collected from 12 roadside stations distributed across four urban roads, with three stations per road, during a standardized dry-season midday sampling period, and were subsequently subjected to alkaline digestion, microscopic screening, and ATR-FTIR confirmation. Of 99 visually suspected particles, 44 were verified as synthetic polymers and retained in the final dataset. Mean atmospheric microplastic concentrations ranged from 0.0079 to 0.0212 items m−3, with J.R. Borja Street showing the highest concentration and Nazareth Street the lowest. Abundance did not differ significantly among roads, whereas particle shape, color, and polymer composition showed significant differences within the confirmed dataset, while size-class distribution did not. Fibers were the dominant morphology (56.8%), transparent particles were the most common color class (52.3%), and polypropylene and polyethylene terephthalate were the predominant polymers. Taken together, the findings confirm the presence of airborne microplastics across roadside environments in Cagayan de Oro City and suggest that, under the sampled conditions, spatial variation was more evident in particle characteristics than in overall abundance. This study contributes an initial polymer-confirmed roadside dataset for a secondary Philippine city and highlights the value of composition-based assessment in urban air quality monitoring.

Microplastics doi: 10.3390/microplastics5020115

Authors: Taiwo Ayorinde Amanda K. Charlton-Sevcik William C. Hockaday Christie M. Sayes

Microplastics (MPs) in wastewater treatment plants are exposed to oxidative conditions during disinfection and advanced oxidation processes (AOPs), which can alter morphology and surface chemistry and influence interactions with coexisting contaminants. Here, accelerated chemical oxidation was simulated using heat-activated potassium persulfate (K2S2O8) and sodium hypochlorite (NaOCl) to examine the oxidative aging of MPs made from polyethylene (PE), polyethylene terephthalate (PET), and polypropylene (PP). Changes in particle morphology and surface chemistry before and after oxidant treatment were characterized using scanning electron microscopy (SEM) for morphological analysis and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy for chemical characterization. Carbonyl formation, an indicator of polymer oxidation, was evaluated using the carbonyl index (CI). Both oxidants induced surface morphological defects and carbonyl functional groups in the MPs, with CI increasing with degradation time. The CI trends suggest that MP oxidation varies with polymer type and oxidant. The effect of oxidative aging on MP sorption capacity was also investigated using copper ions as a model inorganic constituent. Although oxidative aging introduced oxygen-containing functional groups, no statistically significant differences in copper sorption were observed between pristine and oxidized MPs, indicating that MPs can act as vectors for copper regardless of their degree of surface oxidation.

Microplastics doi: 10.3390/microplastics5020114

Authors: Eleonora Monfardini Maria Letizia Costantini Tommaso Valente Laura Ciaralli Giulio Careddu Giovanna Jona Lasinio Daniela Berto Federico Rampazzo Greta Panunzi Maila Severini Martina Radicioli Cecilia Silvestri Marco Matiddi

Microplastic (MP) pollution is an emerging environmental stressor in marine ecosystems, yet its relationship with trophic ecology remains poorly understood in deep-sea environments. This study investigated MP ingestion in relation to trophic ecology in three deep-sea fish species (Chlorophthalmus agassizi, Hoplostethus mediterraneus, and Coelorinchus caelorhincus) from the central Tyrrhenian Sea (Western Mediterranean). Stable isotope analysis (δ13C and δ15N) was combined with detailed characterisation of ingested MPs to assess trophic niches, trophic position, and species-specific ingestion patterns. The three species showed distinct isotopic signatures, with C. agassizi occupying a lower trophic position, while H. mediterraneus and C. caelorhincus overlapped at higher trophic levels. MPs were detected in all species, with an overall frequency of occurrence of 34.4%, and no significant interspecific differences in occurrence or abundance were observed. However, significant differences emerged in MP characteristics. C. caelorhincus, which exhibited the widest isotopic niche, ingested larger and more diverse particles, whereas C. agassizi showed lower occurrence but higher particle loads in affected individuals. These results suggest that trophic ecology is not clearly associated with MP ingestion rates but may influence the size and diversity of ingested particles, highlighting ecological drivers of exposure in deep-sea ecosystems.

Microplastics doi: 10.3390/microplastics5020113

Authors: Mónica G. Silva Adelina Gama Sílvia C. Nunes Mariana Fernandes Maria Manuel Oliveira Francisco Peixoto

Current knowledge on the toxic effects of microplastics (MPs) on human health relies on the extrapolation of data collected from in vivo studies. These studies, however, present limitations, as the particles used often differ from their environmental counterparts. Nevertheless, they provide valuable insights into the mechanisms underlying MPs’ toxicity. In this study, we targeted the mitochondria to investigate the effects of two types of polyethylene microplastics (PE MPs, 27–32 µm), fluorescent and non-fluorescent, on kidneys from FVB/n mice. Animals were exposed for 28 days to two environmentally relevant concentrations of PE MPs (0.002% (w/w) and 0.006% (w/w)). Results reveal that both MPs induce mitochondrial dysfunction, as indicated by oxygen flux depletion in different coupling-controlled states. Complex II dysfunction, particularly at the highest concentration of fluorescent particles, and alterations in other components of the electron transport chain were identified as one of the causes of mitochondrial dysfunction. MPs’ exposure also induced subtle remodelling of the mitochondrial membrane lipid profile, marked by shifts in specific saturated and unsaturated fatty acids, suggesting an adaptive response to preserve membrane integrity. These alterations were accompanied by oxidative stress, evidenced by decreased SOD and CAT activities, particularly under high concentrations of fluorescent PE MPs. Overall, fluorescent MPs triggered stronger mitochondrial and metabolic disruptions in the kidney. All together, these findings reinforce mitochondria as pivotal targets of MPs’ toxicity and highlight the need for improved experimental models that better reflect environmentally relevant exposure scenarios.

Microplastics doi: 10.3390/microplastics5020112

Authors: Jonita Perfanova Lev Tribis Gergana Kuncheva Momtchil Dimitrov Hristo Valchovski Veselinka Petrova Milena Kercheva

Microplastic pollution threatens soil health by disrupting chemical and microbial balances and impairing nutrient cycling, with effects that vary depending on soil properties. The objective of this study is to determine the effects of contamination with three types of microplastics (<5 mm)—polypropylene (PP), polyethylene (PE), and polyethylene terephthalate (PET)—on the microbiological and chemical parameters of four soil types in Bulgaria: Calcic Chernozem, Vertisol, Luvisol, and Fluvisol. A controlled 180-day laboratory incubation experiment was performed, where each soil type was contaminated with microplastics at three concentrations to monitor changes in key microbiological and chemical properties. It was established that microplastics contamination suppressed abundance of key microbial groups and limited nutrient availability, inducing a state of biological and chemical imbalance in the soil. PET exerted the strongest impact on soil chemical properties, with the agrochemical properties of Fluvisol being the most sensitive to MP contamination. PE and PET had the greatest influence on microbial communities, with Vertisol and Luvisol being the most affected in this regard. PP contamination altered key metabolic processes, with the specific impact being highly dependent on soil type and most pronounced in Chernozem. Furthermore, the concentration influenced the measured parameters, with the effects varying depending on the soil type and the microplastic type. Among the factors influencing soil responses to microplastic contamination, soil type appears to be the most decisive, followed by the type of microplastic.

Microplastics doi: 10.3390/microplastics5020111

Authors: Vyoma Jani Shenghua Wu

Following discussions between the Editorial Board and the authors, the original published references 15, 61, and 175 and their citations in the original publication [...]

Microplastics doi: 10.3390/microplastics5020110

Authors: Olga Iakobson Sergey Silonov Viktor Korzhikov-Vlakh Pavel Chelushkin Elizaveta Shtro Vladimir Isakov Natalia Shevchenko

The scientific literature lacks sufficient data on the transport of various toxic pollutants by polymer particles. Investigating how the structure of microplastic particles formed during the degradation of polymeric materials affects pollutant sorption processes will improve our ability to predict environmental behavior. General-purpose polystyrene, expanded polystyrene, ABS plastic (acrylonitrile–butadiene–styrene) and crosslinked polystyrene are produced on an industrial scale. Copolymers of styrene with divinylbenzene are used on a large scale as sorbents for gel permeation chromatography (Styragel brand sorbents), in the production of catalysts on a polymer substrate or ion-exchange resins. In this study, non-spherical, crosslinked polystyrene microparticles with varying polystyrene chain packing densities were used as model microplastic particles representative of crosslinked polystyrene. It was shown that the adsorption of a hazardous chemical rhodamine B was influenced by both the packing density of the polystyrene chains and the presence of ionic functional groups, i.e., the “degree of aging” of the microplastic particles. The sorption capacities of these model microparticles were compared with those of natural origin (silicon dioxide, quartz powder, and microcrystalline cellulose). A viability assay using HEK293 and HeLa cell lines exposed to leachates from both pristine and rhodamine B-loaded microparticles revealed that all unmodified microparticles, regardless of their nature, exhibited no cytotoxicity at concentrations up to 1000 μg/mL. In contrast, microparticles with adsorbed rhodamine B significantly reduced cell viability to 20–40% at concentrations of 100 μg/mL.

Microplastics doi: 10.3390/microplastics5020109

Authors: Alex Graça Contato Carlos Adam Conte-Junior

Microplastic pollution has become a significant environmental concern due to its persistence and widespread impact across ecosystems. These plastic particles (1 μm to 5 mm), originating from larger plastic debris or industrial sources, accumulate in diverse habitats, affecting biodiversity and human health. Microplastics resist natural degradation, posing challenges to both ecological sustainability and waste management strategies. Although numerous studies have explored microbial degradation, most existing research focuses primarily on bacteria, leaving the role of filamentous fungi comparatively underexplored. This represents a significant research gap, because fungi secrete a variety of extracellular enzymes, including laccases, peroxidases, and esterases, which play crucial roles in the breakdown of synthetic polymers. These enzymes facilitate the depolymerization of microplastics by targeting polymer chains and increasing their susceptibility to further microbial degradation. However, the underlying enzymatic mechanisms and their effectiveness in microplastic remediation remain insufficiently characterized. Here, we critically review the potential of filamentous fungi for microplastic biodegradation, emphasizing their oxidative and hydrolytic enzyme systems, biosurfactant production, and mechanisms of adsorption and mineralization. The novelty of this review lies in consolidating the most recent mechanistic insights into fungal-driven depolymerization pathways, integrating them with advances in genetic engineering, bioprocess scale-up, and regulatory perspectives, areas rarely combined in previous reviews. We identify current limitations related to environmental applicability, enzyme accessibility, and the lack of standardized protocols, and propose strategies to overcome these challenges through enzyme immobilization, microbial consortia design, and synthetic biology approaches. By addressing these gaps, filamentous fungi may contribute to the development of sustainable strategies for plastic pollution mitigation and support circular economy approaches toward polymer biodegradation.

Microplastics doi: 10.3390/microplastics5020108

Authors: Raymond Infante Leira Centeno Travis A. Courtney Juan J. Cruz Motta Roy A. Armstrong

Microplastic pollution has become a global concern due to its widespread impacts on organisms and ecosystems. While there have been a few studies quantifying microplastics in inland areas of Puerto Rico, none, to our knowledge, have studied nearshore coastal surface waters. This study, therefore, presents the first assessment of microplastic concentrations and descriptions in the surface waters of La Parguera Natural Reserve, southwestern Puerto Rico. Using 333-micron plankton net trawls, we found low mean ± standard deviation microplastic concentrations of 0.02 ± 0.07 microplastic particles m−3 (95% confidence interval = 0.01 to 0.04 microplastic particles m−3). The most prevalent polymers were high-density polyethylene (48%) and polyethylene (32%), followed by polypropylene (11%) and polystyrene (7%). The most common colors were white (50%), blue (34%), black (8%), red (5%), and colorless (3%). Subsequently, the common structures found were fragments (78%), filaments (12%), films (8%), and fibers (2%). No clear coastal gradient or seasonal patterns were detected (p < 0.05), and mean concentrations were similar to previously surveyed oceanic waters from the Caribbean, suggesting coastal sources of marine microplastics were minimal compared to oceanic sources. This study provides a foundational understanding of microplastics in the coastal waters of La Parguera Natural Reserve and provides critical baseline data for detecting potential future changes in microplastic concentrations.

Microplastics doi: 10.3390/microplastics5020107

Authors: Lisete Fernandes Abderrazzak Ait Bassou José R. Fernandes Pedro B. Tavares

Micro and nanoplastics (MNPs) are increasingly recognized as contaminants in food systems; however, the specific packaging elements responsible for particle release remain poorly resolved. Most studies treat packaging as a single material category, without covering distinct contributions from the different units of modern food contact materials (FCMs). We propose a packaging structure taxonomy based on functional elements: container (C), closure (CL), and functional layers (F), including operational interfaces (+I), designed to enable components attribution of possible origins of plastic fragments in foods and beverages. Through a structured synthesis of the current literature, we map the primary processes leading to MNP generation across these modules, including tribological abrasion at closure contact interfaces, thermally driven polymer degradation in containers and delamination or shedding from coatings, adhesives and multilayer structures. Available evidence indicates that repeated mechanical actions such as opening and closing cycles can generate measurable particle release from closure assemblies. The proposed C/CL/F + I framework introduces standardized descriptors and reporting units that improve comparability across studies and supports origin attribution. By explicitly separating packaging parts and their operational interaction zones, the taxonomy provides a methodological bridge between analytical microplastic detection and engineering strategies aimed at minimizing particle formation. Its adoption can facilitate harmonized experimental design, strengthen regulatory risk assessment and guide the development of packaging configurations that minimize plastic particle shedding into foods.

Microplastics doi: 10.3390/microplastics5020106

Authors: Marko Jugo Christopher M. Reddy Bryan D. James Tarzan Legović

Plastic pellet spills are a growing environmental concern, yet response strategies remain limited and poorly adapted. This study evaluates whether existing oil spill recovery tools, including booms, skimmers, and specialized vessels, can be repurposed to respond to acute releases of plastic pellets at sea. Plastic pellets, although small (typically 1–5 mm in diameter), exhibit variation in physical properties, including polymer type, size, shape, color, and density. These features strongly influence dispersion dynamics and the feasibility of cleanup. Our analysis reveals critical limitations in current response technologies, primarily due to their oil-centric design and lack of consideration for the unique behavior of plastic pellets. By bridging expertise in oil spills and emerging plastic threats, we outline opportunities for adaptive, cross-sector response strategies tailored to the realities of plastic-pellet spills. This study includes a field demonstration in the Northern Adriatic Sea, where oil-spill skimmers and booms were successfully tested for plastic pellet recovery under real-world marine conditions.

Microplastics doi: 10.3390/microplastics5020105

Authors: Elena Bisinicu

Microplastic pollution is a pervasive and ecologically significant threat to aquatic systems. Zooplankton, as key mediators of energy transfer and carbon cycling, are particularly vulnerable to microplastic ingestion due to size overlap with natural prey. This systematic literature review synthesises 250 peer-reviewed studies on zooplankton–microplastic and nanoplastic interactions, identified through a Web of Science search (403 initial records, 2012–2026) and screened using Preferred Reporting Items for Systematic Reviews and Meta-Analyses criteria. Bibliometric and narrative thematic analyses were conducted to evaluate publication trends, taxonomic coverage, biological endpoints, experimental design, particle characteristics, and geographic distribution. Publication output increased rapidly after 2019, with nanoplastics emerging as a major research focus. The literature is strongly biassed toward model organisms such as Daphnia magna and Artemia salina, with limited representation of marine taxa. Ingestion and oxidative stress are the most studied endpoints, while trophic transfer, carbon flux, and multi-stressor interactions remain underexplored. Reported experimental designs are predominantly laboratory-based and frequently employ supra-environmental concentrations and simplified particle types. A major geographic gap is identified for the Black Sea, with minimal coverage and no data for dominant regional species. Future research should prioritise ecologically realistic conditions, broader taxonomic and geographic representation, and integrated multi-stressor approaches to support ecosystem-based management. This review characterises publication patterns and knowledge gaps; it does not constitute a formal evidence synthesis, and frequency distributions reflect research coverage rather than strength of evidence.

Microplastics doi: 10.3390/microplastics5020104

Authors: Ivan Josué Vargas-Lopez Alexandra Vera Anderson Rivadeneira Werner Brämer-Escamilla Gema González Sarah Briceño

Microplastics have emerged as a major environmental health concern due to their environmental persistence, fragmentation, and widespread distribution. Conventional adsorption strategies often have limited efficiency, reuse, and scalability, and may generate secondary pollutants. This work explores the use of ferrotitaniferous sand milled for 4, 8, 12, 16, 32, and 52 h and subsequently functionalized with polyethylene glycol (PEG) for the removal of Polyethylene Terephthalate(PET) microplastics. The samples were characterized using Fourier-Transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman spectroscopy, and scanning electron microscopy (SEM). The average particle size of the samples decreases with the milling time from 60±35 μm to 3±1 μm. The magnetic properties enable rapid separation of sand–microplastic aggregates from water using magnets. Ferrotitaniferous sand exhibits soft ferrimagnetic behavior, with a maximum saturation of 50.09 emu/g. The remanence and coercivity increase as the average particle size decreases. Ultraviolet–visible (UV-Vis) spectroscopy was used to quantify the hydrothermally fragmented PET microparticles in water. The maximum microplastic adsorption removal was 95% within 30 s for the 12 h milled sample coated with PEG. The results show that PEG increases the samples’ adsorption capacity from 20.48 to 32.36 mg/g. The novelty of this work lies in the use of magnetic Ferrotitaniferous sands as a promising, sustainable resource for magnetic separation technologies.

Microplastics doi: 10.3390/microplastics5020103

Authors: Subramani Thirunavukkarasu Manickkam Jayakumar Maduraiveeran Ramachandran Santhosh Jeferson Poovazhagi Rajendran Jishnu Panamoly Ayyappan Murugan Vasanthakumaran Priyanka Muthu Jiang-Shiou Hwang

Microplastic contamination in wetland ecosystems is an escalating environmental threat, compromising ecosystem services, biogeochemical cycling and biodiversity conservation. This study assessed the occurrence, distribution and physicochemical characteristics of microplastics in the Ramsar-designated Pallikaranai wetland, southern India. Six representative subsamples were collected from spatially distinct locations and analyzed using density separation, followed by polymer identification via Raman spectroscopy and energy-dispersive X-ray spectroscopy (EDS). Microplastics were ubiquitously detected across both sediment and water matrices, with significantly higher abundances in sediments, indicating their role as a major sink. The dominant polymer types, polyethylene (PE), polypropylene (PP) and polystyrene (PS), along with prevalent morphotypes such as fragments, fibers, beads and foams, reflect diverse and persistent anthropogenic inputs. The compositional profile strongly implicates mismanaged domestic and urban waste as the primary source. The widespread presence and accumulation of microplastics in this ecologically sensitive wetland raise concerns over potential impacts on trophic interactions, habitat quality and long-term ecosystem resilience. These findings underscore the urgent need for targeted waste management strategies, pollution mitigation frameworks and continuous monitoring to safeguard the ecological integrity of the Pallikaranai wetland and similar Ramsar-listed ecosystems.

Microplastics doi: 10.3390/microplastics5020102

Authors: Hriddhi Sarker Goutam Saha Awnon Bhowmik Amlan Ganguly

Microplastics (MPs) are increasingly detected environmental contaminants in both marine and freshwater ecosystems, with reported concentrations ranging from a few to thousands of particles per cubic meter depending on location and methodology. Although growing evidence suggests potential risks to aquatic organisms, the extent of their ecological and biological impacts is still under active investigation. Their size, persistence and capacity to transport chemical additives and co-contaminants allow them to enter biological systems by ingestion and respiration. When ingested, MPs cause oxidative stress, inflammation, and metabolic disorders, resulting in the destruction of vital tissues in major body organs including liver, gills, intestines, and brain. They also change gene expression, cause endocrine and immune pathway perturbation, induce apoptosis, and cause gut microbiome dysbiosis, all of which worsen the health and survival of the organism. MPs also serve as vectors of heavy metals, antibiotics, pesticides, and pathogens and enhance toxicity due to the Trojan horse effect and enable bioaccumulation in food webs. Due to their widespread presence in water, soil, air, and food, MP pollution has direct effects on human, animal, and ecosystem health. This review synthesizes current knowledge on the sources of MPs, the mode of exposure, and the mechanism of toxicity and new ecological implications. It also presents mitigation measures, and stresses a One Health paradigm as the key to taking concerted action on the international level to minimize MP pollution and protect both the environment and human health.

Microplastics doi: 10.3390/microplastics5020100

Authors: Md Imran Hossain Md Shofiqul Islam Yi Zhang Alessandra Sutti Zoran Najdovski Mohammad Anwar Hosen Maryam Naebe

The widespread release of microplastics (MPs), especially fibrous microplastics (FMPs) originating from synthetic textiles, poses a growing threat to environmental systems due to their persistence, mobility, and potential for bioaccumulation in aquatic and terrestrial ecosystems. Conventional gravimetric methods (GMs) remain the primary approach for assessing FMP shedding, yet they are hindered by moisture-sensitive filters, false positives from detergents and minerals, environmental contamination, and the labor-intensive manual measurement of individual fibers. To address these limitations, we developed an automated image analysis (AIA) framework that integrates an attention-based U-Net architecture with meta-learning modules to quantify FMP number, length, diameter, and mass from stitched microscopic images of entire filter membranes. This approach enables detection of fibers down to 28 μm in diameter with the spatial resolution of 2.17 µm/pixel, supports both target-color and multi-color analysis, and eliminates the need for manual characterization or extrapolation from partial membrane segments. The method achieved the highest accuracy of approximately 98% in color-specific fiber detection, correctly identifying 257 of 263 white fibers, and demonstrated similarly robust performance for black, red, and green fibers, while minimizing interference from non-target colors, even when their fibers overlapped. Multi-color detection was further validated using effluent water samples containing mixed-color fibers. Overall, the developed system enhances the accuracy, efficiency, and reproducibility of FMP analysis, offering a standardized and scalable approach for environmental monitoring of MP pollution.

Microplastics doi: 10.3390/microplastics5020101

Authors: Teresa Fracchiolla Stefania Nunzia Lisco Angela Rizzo Corrado Sasso Francesco Veneziano Roberta Trani Alessia de Luca Angela Stufano Giusto Lo Bue Massimo Moretti

This study investigates the distribution and concentration of microplastics (MPs) across the littoral profile of a beach, from dune base to offshore sector, including an estuarine channel and Sabellaria alveolata bioconstructions. The research was conducted at Pino di Lenne beach (Taranto, Ionian Sea), a wave-dominated, microtidal littoral system representing a unique natural laboratory with minimal anthropogenic pressure. An eco-friendly extraction protocol was used, combining methods that were already known in the literature. Olive oil proved highly effective in isolating a wide range of MP densities from sediment samples. Statistical analysis identified key accumulation zones, with the highest mean concentrations found in the submerged sandbar (2435 MPs/kg), Sabellaria bioconstructions (2324 MPs/kg), and the base of the dune (2065 MPs/kg). Fibres were the predominant morphology across all sub-environments. Distribution is interpreted as controlled by hydrodynamic processes and biological activity. The submerged beach drives MP transport, with the sandbar and shoreface acting as dynamic sinks. Sabellaria bioconstructions function as biological trap, actively incorporating MPs into their tubular structures. The dune base acts as a sink for wind-blown and storm-deposited plastics. These sub-environments function as critical littoral traps for MPs, essential for developing targeted monitoring and remediation strategies in similar coastal systems.

Microplastics doi: 10.3390/microplastics5020099

Authors: Aleksandra Bozic Branka Hadzic Zorica Lazarevic Milica Curcic

Sunlight-driven UV weathering is a major transformation pathway of environmental microplastics, promoting surface oxidation, molecular degradation, embrittlement, and progressive fragmentation toward smaller size fractions. However, comparisons across studies remain difficult because weathering is often described using descriptors that probe different aspects of degradation without being clearly distinguished. Surface-sensitive oxidation metrics, such as carbonyl or oxidation indices (CI/OI), are frequently emphasized, whereas fragmentation and embrittlement are more directly governed by bulk molecular-weight loss, mechanical weakening, and particle-size evolution. This review examines UV weathering of common polymers through a coupled chemico-mechanical perspective relevant to the micro-to-nano transition. We distinguish surface chemical descriptors, bulk molecular and mechanical descriptors, and fragmentation-related metrics, and critically assess the analytical methods used to measure them, including FTIR, Raman spectroscopy, GPC/SEC, thermal methods, mechanical testing, and particle-size analyses. We argue that no single metric is sufficient to describe weathering progression, and that meaningful interpretation requires joint reporting of oxidation state, Mn/Mw changes, mechanical deterioration where available, and particle-size distribution as a function of cumulative or spectrum-weighted UV dose. We further propose a minimal QA/QC reporting framework including UV metadata, temperature, oxygen availability, blanks, replicates, recovery tests, and matrix-specific detection limits. By separating what different methods actually probe and linking them to fragmentation mechanisms, this review provides a more operational basis for interpreting UV-aged microplastics in environmental sampling and biomonitoring.

Microplastics doi: 10.3390/microplastics5020098

Authors: Mina Popovic Luka Dragacevic Milan Kojic Daria Tsibulskaia Neveka Rajic

This study highlights the strong ability of a new bacterial strain, Hafnia paralvei UUNT_MP29, isolated from the gastrointestinal tract (GIT) of common carp (Cyprinus carpio), to break down polystyrene (PS). As an omnivorous bottom feeder, C. carpio is constantly exposed to microplastics, creating a unique environment that favors the evolution of specialized microbiota capable of degrading polymers. Genomic analysis of the isolate identified key homologs involved in xenobiotic breakdown, including alcohol dehydrogenase (Adh), 3-hydroxybutyrate dehydrogenase (HDH), and a small glutamine-rich tetratricopeptide repeat-containing protein (SGTA), showing a strong metabolic system for processing long-chain hydrocarbons. Growth experiments showed the strain quickly adapted, reaching maximum cell density and forming mature biofilms by Day 16. Gravimetric analysis confirmed that H. paralvei UUNT_MP29 uses PS as its primary carbon source, with a significant weight loss of 16.76% over 16 days. Kinetic modeling indicated the degradation follows first-order kinetics (R2 = 0.9243) with a high degradation rate constant (k) of 0.2078 day−1. Surface analyses using FTIR and SEM confirmed extensive oxidative changes, as evidenced by the rising Carbonyl Index and surface erosion. TGA also showed reduced thermal stability of the treated polymer, suggesting microbial chain scission. These findings demonstrate the strong degradative ability of H. paralvei UUNT_MP29 and highlight the GIT of plastic-exposed aquatic animals as a promising area for discovering powerful biocatalysts for microplastic cleanup.

Microplastics doi: 10.3390/microplastics5020097

Authors: Fabio D’Ottaviano Kyle Hart

Detection and quantification of microplastics are undermined by the absence of appropriate methods in some contexts—such as a statistically principled LOD for particle-count data—but more pervasively by the uncritical application of ostensibly simple rules outside the conditions under which they are valid. This paper examines the statistical and study-design decision points where such failures most commonly occur and articulates principled constraints on valid inference at each step. Specifically, it (1) distinguishes exploratory from confirmatory research and advocates preregistration to prevent HARKing; (2) argues for field-blank-based limits to improve internal validity; (3) shows how multiple simultaneous comparisons against the LOD inflate the family-wise error rate and how to adjust α accordingly; (4) demonstrates that the LOD multiplier kD depends on blank sample size and critiques fixed-multiplier heuristics lacking statistical justification; (5) examines the consequences of distributional misspecification for LOD estimation; (6) demonstrates that an LOD for summed polymer concentrations exists only under restrictive conditions; (7) clarifies why subtracting limits from individual measurements is not quantification, and that cohort-level inference requires a median comparison via log-transformed data with a corresponding confidence interval; and (8) introduces a Bayesian framework for particle-count LODs that accounts for partial filter inspection. These discussions are summarized in a minimum reporting checklist designed as an evaluative aid—not a prescriptive recipe—to help researchers make analytical choices explicit and support reviewers in assessing methodological validity.

Microplastics doi: 10.3390/microplastics5020096

Authors: Ignazio Allegretta Concetta Eliana Gattullo Mohammad Yaghoubi Khanghahi Carlo Porfido Fani Sakellariadou Carmine Crecchio Matteo Spagnuolo Roberto Terzano

Soils are increasingly affected by microplastic (MP) contamination, mainly coming from industrial activities, agricultural practices, atmospheric or waterborne transport, and improper waste disposal. Despite the increasing attention to the fate of MPs in soil over the last few years, research in this area is still limited compared to aquatic ecosystems. The introduction of MPs into the soil environment can modify not only the soil properties but also the interactions among soil components, plants, and microorganisms, thus affecting the mobility and availability of other contaminants, such as potentially toxic elements (PTEs). This review critically examines the complex dynamics between MPs and PTEs in the soil ecosystem, with a focus on the conditions under which MPs can act as a source or a sink of PTEs. Indeed, on the one hand, MPs can adsorb or complex PTEs on their surfaces (similarly to natural soil colloids), thus reducing their mobility and availability; on the other hand, they can release/mobilize PTEs after MP degradation or act as micro-/nano-vectors of PTEs. Understanding such mechanisms is relevant when evaluating the environmental risks associated with the co-presence of MPs and PTEs in soil, a situation likely to occur in most contaminated sites and in many agricultural soils.

Microplastics doi: 10.3390/microplastics5020095

Authors: Gea Oliveri Conti Paola Rapisarda Eloise Pulvirenti Giovanna Deiana Maria Anna Coniglio Giuseppe Mancini Paolo Castiglia Antonio Azara Margherita Ferrante Marco Dettori

Plastics are now ubiquitous in the environment, in this, the “Plasticene” era. Microplastics (MPs) and Nanoplastics (NPs) are emerging contaminants of global concern. This systematic review, registered on PROSPERO and conducted according to PRISMA guidelines, investigated the presence, distribution, and characteristics of MPs in tap water (TW) worldwide, and estimated the population’s Estimated Daily Intake (EDI) by age group, including pregnant women. A comprehensive search across PubMed, Scopus, Web of Science, and Cochrane identified 22,650 records, of which 8 studies were included. MPs were detected in treated water (TW) in the studies included in this review, although the currently available evidence remains limited. Calculated EDIs were highest in children aged 6 months to 3 years (up to 39 MPs/kg bw/day), followed by pregnant women (up to 14.96 MPs/kg bw/day), reflecting differences in water intake per body weight. These estimates must be interpreted as indicative, estimated by methodological variability among studies. The widespread presence of MPs in TW calls for standardized methods, improved treatments, and thorough monitoring to assess risks and protect public health.

Microplastics doi: 10.3390/microplastics5020094

Authors: Aleksandra Blazhenko Anastasiia Mikhel Marina Kostina Mikhail Goikhman Pavel Chelushkin Andrey Korenevsky

There are currently no published methods for the controlled introduction of microplastic particles into the European rabbit (Oryctolagus cuniculus) as an animal model. The aim of this pilot study was to establish a novel rabbit-based experimental model for assessing the impact of microplastic particles by evaluating the physiological and biochemical responses to an eight-day oral administration of polystyrene latex (1 and 5 mg/kg/b.w./day), providing a foundation for future studies. This study was also aimed at evaluating the possibility of using Raman spectroscopy and Fourier-transform infrared spectroscopy to analyze the distribution of microplastics in rabbit samples. We observed a dose-dependent decrease in water and food consumption in the high-dose (5 mg/kg) study group. In addition, a decrease in alanine aminotransferase and total calcium levels, along with an increase in phosphorus levels, was detected. The rabbit’s stomach was the only organ where polystyrene microparticles were identified, with the colon, kidneys, ovaries, and uterus not showing any evidence of polystyrene presence. The selected doses of microplastics did not lead to pronounced toxic effects in rabbits and may be used on larger animal samples. Physiological and biochemical data obtained indicate predominantly negative metabolic shifts associated with the intake of microplastics, which warrants further study.

Microplastics doi: 10.3390/microplastics5020093

Authors: Kuok Ho Daniel Tang

Micro- and nanoplastic (MNP) pollution has emerged as a global environmental and health concern, driving the rapid development of sensor technologies for faster, more sensitive, and field-deployable detection. This review synthesizes recent advances in optical, electrochemical, and biosensor platforms for MNP analysis and compares their analytical performance and practical feasibility. Optical sensors, including plasmonic, spectroscopic, and colorimetric systems, enable label-free and often rapid detection with material discrimination capability, and are well-suited for screening applications, though they commonly exhibit higher detection limits and matrix interference. Electrochemical sensors demonstrate the highest analytical sensitivity overall, frequently reaching low µg L−1 to ng mL−1 levels, with strong potential for miniaturization and on-site deployment; performance is further enhanced by nanostructured electrodes, photoelectrochemical designs, and signal amplification strategies. Biosensors incorporating peptides, aptamers, enzymes, or engineered proteins provide improved polymer selectivity and enable targeted detection, but face challenges related to stability, cross-reactivity, and reproducibility in complex samples. Practically, portable electrochemical and simple optical colorimetric platforms are currently the most feasible for field use, while hybrid bio-electrochemical systems show the highest performance potential. Future research should prioritize robust selective recognition elements, antifouling interfaces, standardized validation protocols, mixed-polymer quantification models, and integration with machine learning to enable reliable, real-world MNP monitoring.

Microplastics doi: 10.3390/microplastics5020092

Authors: Timur Nizamutdinov Ivan Kushnov Anastasia Vainberg Evgeny Abakumov

Soil micro- and nanoplastic contamination is escalating globally, yet its potential to interfere with routine agrochemical analyses remains poorly quantified. Standard operating procedures (SOPs) were calibrated for natural soil matrices and may not account for synthetic, carbon-rich polymers. This controlled model study quantified the analytical sensitivity of FAO/GLOSOLAN/ISO standard procedures to polystyrene nanoparticle (50 nm) contamination across a 0–0.5% (w/w) gradient in a Luvic Chernozem. Key parameters—pH, soil carbon, total nitrogen (TN), cation exchange capacity (CEC), and clay fraction—were measured following standardized protocols. The Walkley–Black method exhibited a strong dose-dependent increase in measured SOC (r = 0.93), reflecting systematic overestimation due to dichromate co-oxidation of polymer matrix, likely facilitated by exothermic heating above polystyrene’s glass transition temperature. The Dumas method showed moderate correlation (r = 0.59) but higher replicate variability driven by small aliquot size and heterogeneous nanoparticle distribution. The pH measurements displayed non-linear responses and elevated variability at low doses, whereas TN, CEC, and clay content remained statistically stable. These findings demonstrate that nanoplastic contamination can introduce significant analytical artifacts in oxidation-based SOC determinations, potentially leading to misinterpretation of soil carbon trends. Given the single-soil, single-polymer design, results represent a system-specific proof of analytical vulnerability rather than a universally quantified bias. Laboratories analyzing potentially contaminated soils should exercise caution with wet-oxidation SOC data, and broader SOP revisions must await multi-soil, multi-polymer validation campaigns.

Microplastics doi: 10.3390/microplastics5020091

Authors: Luciana Banquero Paul E. Sacks Fnu Joshua Lei Zhai Joshua S. Sacks Linda J. Walters

Many filter-feeding invertebrates consume microplastics (MP) under laboratory conditions, but little is known about newly settled, field-collected juveniles. To address this information gap, we collected 3439 juvenile invertebrates in the Indian River Lagoon (IRL), FL, USA. Previous studies suggest that the IRL is a MP hotspot. A total of 70% of IRL adult oysters (Crassostrea virginica) contained MP (mean: 2.3 MP/individual), and MP number and MP length were positively correlated with animal size. We predicted that juvenile C. virginica and other sessile invertebrates would contain MP with a positive correlation to animal size. Five species were examined; 51% were C. virginica (mean shell length ± SD: 6.3 ± 4.7 mm). Overall, 117 (3.4%) animals contained potential MP (fibers: 90.7%). Of these particles that matched FTIR databases with a score of 70% or greater, 51% were plastic and 49% were anthropogenically modified particles. No correlations to animal size were found for particle presence (logistic regressions: p ≥ 0.20 for all species) or particle length (linear regressions: p ≥ 0.23 for all species). Thus, even though found in a MP hotspot, our extrapolated results suggest few juveniles (<1%) contained MP. This information is important for understanding the relationship between MP and the life histories of filter-feeding animals, especially for species considered biological indicators of MP.

Microplastics doi: 10.3390/microplastics5020090

Authors: W. Rosado-Martínez B. Pamplona K. García-Uitz J. C. Cruz L. G. Arriaga J. Ledesma-García M. P. Gurrola

Microplastic pollution is a major environmental concern due to its persistence, global distribution, and potential impacts on ecosystems and human health. This systematic review, conducted according to PRISMA 2020 guidelines, analyzes research trends in microplastics with a focus on physicochemical characterization and removal technologies. A literature search was performed in Scopus and Web of Science (1972–2025) using predefined inclusion and exclusion criteria. After screening and duplicate removal, 89 studies were included in the final analysis. It is considered that with this dataset, it is possible to capture the main analytical and technological developments in the field. As a bibliometric-oriented study, no formal risk-of-bias assessment was conducted. However, a qualitative consideration of potential biases was undertaken, particularly regarding publication bias, database coverage limitations, and the predominance of English-language peer-reviewed studies. These aspects were considered when interpreting the results. The Methodi Ordinatio approach was then used to rank publications based on scientific relevance and citation impact. Results show the predominance of FTIR and Raman spectroscopy for microplastic characterization, while removal technologies remain heterogeneous and less standardized, with most approaches still at laboratory scale. Key gaps include the lack of standardized analytical protocols and limited integration between detection and remediation strategies. Overall, this review highlights critical research trends and supports the development of scalable solutions for microplastic pollution.

Microplastics doi: 10.3390/microplastics5020089

Authors: Aswir Abd Rashed Nurliayana Ibrahim Mohammad Adi Mohammad Fadzil

Introduction: Microplastic (MP) contamination can endanger marine ecosystems and indirectly affect the well-being of humans through the ingestion of marine species. While most research investigates the digestive system, such as the gills and gastrointestinal tract of fish, it still fails to address a major oversight in understanding MP deposition in edible tissues, which is the primary route of human exposure. The differences in contamination levels among pelagic, demersal, and benthic fish in Malaysian waters remain poorly understood. This preliminary study uses Laser Direct Infrared Imaging (LDIR), a new, high-resolution, automated technique, to examine synthetic MP contamination in the edible portion of fish. Materials and Methods: The MPs were extracted from the edible tissue of three fish species representing pelagic (Fish A), benthic (Fish B), and demersal (Fish C) using KOH and sieved onto a gold mesh filter before analysis using LDIR. Results and Discussion: LDIR identified 162 MP particles, revealing clear differences by polymer type and habitat. Pelagic species mostly contained polyethylene (PE) and rubber (n = 8). Demersal species had mostly polyethylene terephthalate (PET) with small amounts of PE and rubber (n = 57). Benthic species showed the highest load, dominated by PET and polypropylene (PP) (n = 97). The morphological assessment of the MPs indicated that the polymers in pelagic fish were smaller, with an area of 2047.82 µm2 and a circularity range of 0.14–0.74, indicating consistent shape. Conversely, MPs are irregular and larger in benthic fish, with areas up to 38,837.50 µm2 and circularities ranging from 0.02 to 0.81. This pattern reflects specific accumulation related to habitat and potential environmental degradation processes. Conclusions: This preliminary study demonstrates the effectiveness of LDIR for detecting MPs in edible fish tissues. The findings provide a fundamental dataset on MP contamination in edible tissue and emphasize its distribution across ecological zones. Nevertheless, broader research is required to substantiate these data and assess the implications of MP contamination for the environmental stability of human and marine well-being.

Microplastics doi: 10.3390/microplastics5020088

Authors: Jose Javier Flores Laura Cortés-Corrales Adrián Rosa García Alfredo Alcayde Amadeo R. Fernández-Alba Maria Jesús Martínez Bueno

Microplastics (MPs) in water treatment plants (WTPs) represent a critical environmental concern, particularly when treated effluent is reused for agricultural irrigation. This study investigates the occurrence, removal efficiency, and characterization of MPs in tertiary-treated wastewater destined for agricultural reuse in water-scarce regions. Additionally, the study examines the influence of sample volume on extrapolated MP concentrations. Despite advanced treatment processes including ultrafiltration achieving removal efficiencies of 89%, substantial quantities of MPs remain in final effluents at concentrations ranging from 89 to 399 MPs/m3 (equivalent to 0.1–0.4 MPs/L) with a mass load of 2 µg/L at the outlet. Morphological analysis revealed a shift from fragment-dominated influent (~50%) to film-dominated effluent (~51%), with blue particles being most prevalent. Size distribution analysis showed distinct peaks: 50–100 µm for fragments, 100–250 µm for films, and 250–500 µm for fibres. Polytetrafluoroethylene (PTFE) emerged as the dominant polymer across all morphotypes. Finally, converting particle counts to mass loads indicated an average decrease from ~11 µg/L at the inlet to ~2 µg/L at the outlet, underscoring that number- and mass-based metrics provide complementary information for risk assessment.

Microplastics doi: 10.3390/microplastics5020087

Authors: Matheus Sousa Silva Katerin Manuelita Encina Oliva Márcio Rocha Francelino Alexandre ten Caten

With the global increase in microplastic pollution, even environments considered pristine have shown signs of being affected by these contaminants. In this context, it becomes essential to conduct studies that identify and quantify the presence of microplastics in remote regions such as Antarctica. This continent is particularly relevant due to its low anthropogenic influence and its essential role in regulating planetary ecosystems and biodiversity. In this study, 49 Antarctic samples were analyzed using pretreatment techniques with NaCl and ZnCl2 saline solutions, followed by fluorescence microscopy using Nile Red dye to estimate the microplastic abundance index. Both solutions showed good performance in the separation and identification of particles. Approximately 37% of the samples showed contamination by potential microplastics (PMPs), with a higher concentration of particles retained on paper filters and fibers observed in the supernatants. The results indicate that the presence of MPs in Antarctica is irregular and not ubiquitous, differing from other studies that suggest a wider distribution. It is speculated that the observed contamination results from oceanic transport from other regions of the planet and from sources associated with human activities on the Antarctic continent (e.g., tourism and research).

Microplastics doi: 10.3390/microplastics5020086

Authors: Kristen A. Marcellus Michal Scur Michael G. Wade Santokh S. Gill

Rising plastic production worldwide is contributing to the increasing amounts of micro- and nanoplastics found in the environment. The consumption of microplastics by humans is plausible due to the presence of plastic particles in various food commodities, yet the potential impact of microplastics on human health remains unknown. Several studies have detected microplastics in human tissues and research using mammalian in vivo and in vitro models have noted toxicity after exposure to microplastics. Using both mono- and co-culture liver cell models, we assessed the impact of environmentally relevant, cryo-milled plastic particles on hepatotoxicity. We observed that only cryo-milled polyethylene terephthalate and polystyrene altered mitochondrial energy metabolism, while the other plastic particles did not. The pristine, spherical polystyrene particles were taken up at all sizes and cryo-milled polystyrene was taken up by cells. Evidently, polymer type and shape play a critical role in hepatotoxicity. Further research is required to fully elucidate the effect the physiochemical properties of plastic particles may have on toxicity.

Microplastics doi: 10.3390/microplastics5020085

Authors: Maria Râpă Carmen Gaidău Ecaterina Matei Florin-Aurel Dincă

Blended polyester (PET)-based textiles comprise a significant portion of post-consumer waste, posing substantial challenges to circular economy initiatives while contributing to microfiber (MF) pollution. Despite the considerable recycling potential of PET textiles, no commercially viable technologies currently exist that can efficiently separate and recycle blended PET-based textile waste on an industrial scale. This review provides a comprehensive analysis of recycling strategies for post-consumer blended PET-based textiles and their subsequent valorization pathways. Mechanical, chemical, and biological recycling processes are mostly not yet market-ready, although chemical approaches are considered particularly promising. The findings highlight a critical need for advanced sorting technologies, enhanced material traceability, and robust MF mitigation strategies to foster circularity and contribute to the United Nations Sustainable Development Goals (SDGs). The results further indicate that mechanical recycling of blended PET textiles leads to significant MF release due to fiber fragmentation, whereas chemical recycling offers the potential for improved material recovery, but remains limited by high energy demand and solvent-related challenges. While closed-loop approaches support true circularity by maintaining textile-to-textile material flows, open-loop pathways repurpose textile waste for high-value non-textile applications.

Microplastics doi: 10.3390/microplastics5020084

Authors: Immacolata Liotta Roberto Avolio Rachele Castaldo Federico Olivieri Gennaro Gentile Andrea Sorrentino Andrea Camedda Giuseppe Andrea de Lucia Maria Emanuela Errico Mariacristina Cocca

Plastic waste is estimated to represent 40–80% of the total amount of marine litter, with polyethylene (PE) and polypropylene (PP) being the most abundant polymeric components. The recovery and recycling of marine plastic debris are therefore essential to mitigate environmental pollution and limit the generation of secondary microplastics. In this work, a mechanical recycling strategy was investigated for the valorization of a polyethylene-rich plastic fraction (PE-rf) recovered from the marine environment, characterized by high heterogeneity and persistent inorganic contamination. Different pre-treatment routes, including cryogenic grinding and planetary ball milling, as well as blending approaches with recycled polyethylene and compatibilizing additives, were explored. The effects of composition and processing on the thermal, mechanical, and morphological properties of the resulting materials were systematically analyzed. The results show that intense mechanical homogenization and chemical compatibilization are not sufficient to overcome the intrinsic limitations imposed by contamination and compositional variability. As a proof of concept, selected formulations were processed into filaments and tested in fused filament fabrication, demonstrating basic 3D printability.

Microplastics doi: 10.3390/microplastics5020083

Authors: Phyo Bo Bo Aung Yuya Haga Sota Manabe Wakaba Idehara Mii Hokaku Yuto Motoyama Ayaha Mori Kazuma Higashisaka Yasuo Tsutsumi

Microplastics (MPs), i.e., plastic particles <5 mm, and nanoplastics (NPs), i.e., plastic particles <1 µm, are widespread in the environment. MPs and NPs (MNPs) have also been detected in human tissues. Environmental MNPs exhibit diverse physicochemical properties such as size, shape, and surface degradation. However, most experimental studies have used pristine MNPs, which poorly represent real-world conditions, and only a limited number of studies have focused on preparing environmentally relevant MNPs. Therefore, we focused on the key physicochemical properties of MNPs, particularly their shape, size, and surface degradation, using polyvinyl chloride (PVC) as the model polymer. In this study, fragment and spherical PVC-MNPs were utilized, and surface degradation was introduced through exposure to vacuum ultraviolet (VUV) radiation at a wavelength of 172 nm. Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) analysis revealed the formation of additional carbonyl groups after VUV exposure. We investigated the cytotoxic effects of the degraded and non-degraded PVC-MNPs on A549, Caco-2, and THP-1 cells. The results indicated that the degraded PVC-MNP-treated groups induced higher cytotoxic effects than those in the non-degraded groups. Notably, the degraded PVC-NPs induced stronger cytotoxicity than the degraded PVC-MPs. These findings highlight the potential health risks associated with environmental MNPs.

Microplastics doi: 10.3390/microplastics5020082

Authors: Domenica Mosca Angelucci Marco Manetti Enrica Donati Maria Concetta Tomei

This study investigates the sorption process of selected pollutants, namely naphthalene (NAP), pentachlorophenol (PCP), sulfamethoxazole (SMX), and ibuprofen (IBU), onto different real microplastics (MPs) under controlled laboratory conditions. Sorption tests reveal variable affinities depending on the chemical and physical interactions between polymers and pollutants. NAP showed the greatest uptake on the majority of tested MPs, followed by PCP and SMX, while IBU exhibited negligible sorption. Kinetic tests indicate a general rapid initial uptake, followed by lower sorption rates leading to equilibrium within days. Theoretical thermodynamic affinity estimations, based on the Hansen solubility parameter (HSP) method, previously tested only on antibiotics, are applied for the first time to commercial MPs and several pollutant categories such as PAHs, pesticides and other pharmaceuticals. Predictions have been validated with experimental results and generally show very good agreement with the affinity ranking derived by experimental data. However, some limitations occur due to the heterogeneity of the real MPs and different environmental conditions. Factors affecting, to different extents, MPs’ uptake include hydrophobicity and electrostatic forces, as well as pH and particle size. This work advances understanding of MPs’ role as vectors of pollutants in aquatic environments and validates the use of an innovative combined experimental–theoretical approach useful as a tool to predict associated risk.

Microplastics doi: 10.3390/microplastics5020080

Authors: Xingru Yin Yi Jing Peiwen Zeng Congcong Li Yue Shi Jinyi Zhang Lingjun Yan Wei Sun Guowei Pan

Introduction: It is accepted that nano- and micro-plastic (NMP) pollutants threaten ecosystems and human health by their bioaccumulation but, interestingly, their toxicity is shape-dependent. However, a clear definition of irregular NMPs, as the dominant shape in environmental and biological samples, is currently lacking when compared to spherical and fibrous NMPs. Objectives: This study quantifies morphometric descriptors in order to develop a standardized definition for irregular NMPs. Methods: Hyperspectral images of 34 spherical, 50 fibrous, and 45 irregular NMPs were collected from the literature. All shape-related features reported previously were analyzed using a machine learning model. Using five-fold cross-validation, a decision tree-based ensemble classifier with fixed parameters and Gini coefficient was established to screen key morphometric descriptors and their optimal interval ranges. The model was independently validated, enabling the accurate distinction of irregular NMPs from spherical and fibrous NMPs. Results: Three morphometric descriptors, including circularity, roundness, and perimeter-to-area ratio, were identified using five-fold cross-validation as optimal indicators for NMP shape classification. Optimal interval ranges for irregular NMPs were as follows: circularity (0.388 ± 0.004–0.768 ± 0.004), roundness (0.248 ± 0.01–0.752 ± 0.06) and perimeter-to-area ratio (>11.608 ± 1.39). This approach generated a 96.0% macro-averaged accuracy across these NMPs, with 100% precision and 89.0% recall. Conclusions: Irregular NMPs may be characterized using three morphometric descriptors, such as circularity, roundness, and perimeter-to-area ratio. The three-descriptor combination has highly accurate discrimination from spherical and fibrous NMPs.

Microplastics doi: 10.3390/microplastics5020081

Authors: Vesela Yancheva Stela Stoyanova Elenka Georgieva Desislava Arnaudova László Antal Ifeanyi Emmanuel Uzochukwu Bartosz Bojarski Carlos Gravato Krisztián Nyeste

Microplastic (MP) contamination is increasingly recognized as a global environmental problem affecting aquatic ecosystems, food quality, and animal and human health. Farmed fish represent an important and increasing component of the human diet. Therefore, understanding potential human exposure to MPs is essential for ensuring food safety. In the current paper, we present the results of a preliminary study conducted in Bulgaria on MP contamination in the muscle tissue of rainbow trout [Oncorhynchus mykiss (Walbaum, 1792)] reared in freshwater aquaculture systems. Edible tissues were analyzed using Laser Direct Infrared (LDIR) imaging spectroscopy, a highly sensitive method enabling rapid detection and accurate identification of polymer types present in samples. MPs were detected in all examined specimens, demonstrating that these particles are bioavailable and capable of accumulating in fish muscle tissues commonly consumed by humans. Moreover, the presence of multiple polymer types suggests diverse contamination sources within aquaculture environments. Although the present findings do not allow direct conclusions about human health risks, they indicate potential risks of trophic transfer and highlight the need for improved monitoring strategies and management practices in farmed fish production. Overall, this study provides novel data on MP exposure in aquaculture species and emphasizes the preventive importance of assessing plastic pollution in fish intended for human consumption.

Microplastics doi: 10.3390/microplastics5020079

Authors: Subrata Saha Rachita Saha Manjil Gupta Debangana Saha Ananya Paul Surovi Roy Alolika Bose Sulagna Chandra Koustav Kundu Elena I. Korotkova Muhammad Saqib Pradip Kumar Kar

Microplastic (MP) and nanoplastic (NP) pollution has emerged as a pervasive and still insufficiently quantified pressure on terrestrial ecosystems, yet its consequences for wild herbivores remain incompletely understood. As key links between primary producers and higher trophic levels, wild herbivores occupy a critical ecological position and may serve both as exposed receptors and as biological vectors of plastic contamination. This manuscript presents a narrative review that synthesizes recent advances in understanding the physiological, behavioural, and ecological implications of MP and/or NP exposure in free-ranging herbivorous mammals, integrating evidence from field surveys, experimental studies, ecological modelling, and supportive mechanistic findings from livestock and experimental mammalian systems. Available evidence indicates that MPs and NPs are consistently detected in wild herbivores from both human-modified and protected landscapes, demonstrating widespread terrestrial exposure. Reported biological effects include oxidative stress, digestive dysfunction, inflammatory and immune responses, altered gut microbial communities, impaired nutrient assimilation, and organ-level damage, although much of the mechanistic evidence derives from controlled laboratory or livestock-based studies rather than direct wildlife investigations. Behavioural responses remain comparatively underexplored, particularly in large-bodied herbivores, with limited evidence for altered foraging, habitat use, and stress-related behaviours. At the ecosystem level, emerging studies suggest that herbivores may contribute to the landscape-scale redistribution of MPs and NPs through movement and faecal deposition, with potential downstream effects on soil processes, nutrient cycling, and plant–herbivore interactions. However, the current evidence base is constrained by major methodological and conceptual limitations, including the lack of standardized detection and reporting protocols, limited ecological realism in exposure studies, taxonomic and geographic biases, and poor resolution of long-term population-level and food-web consequences. Overall, the available literature indicates that MP and NP pollution represent a multifaceted and emerging risk to wild herbivores and the ecosystems they inhabit. Future research should prioritize standardized contamination-controlled monitoring, non-invasive faecal surveillance, ecologically realistic chronic exposure studies, and integrated conservation frameworks that recognize wild herbivores as sentinel species for terrestrial plastic pollution.

Microplastics doi: 10.3390/microplastics5020077

Authors: Dércia Santos Ana Luzio João Sousa Ana Rita Pinto Edna Cabecinha Simone Varandas Sandra M. Monteiro Maria José Saavedra

Microplastics (MPs) and metals frequently co-occur in aquatic environments, yet their combined effects on gut health and antimicrobial resistance in fish remain poorly understood. This study investigated the chronic effects of polyethylene (PE) and polystyrene (PS) microplastics, alone or combined with copper (Cu), on intestinal integrity, the gut-associated Gram-negative cultivable fraction, and phenotypic antimicrobial resistance in adult zebrafish (Danio rerio). Fish were exposed for 21 days to MPs (1 mg/L), Cu (25 µg/L), or their combinations. Histopathological analysis revealed that Cu-containing treatments induced more severe intestinal alterations, including edema, villus degeneration, and necrosis, whereas MPs-only exposures produced milder and heterogeneous responses. The composition of the Gram-negative cultivable fraction varied among treatments, with Cu, particularly in combination with MPs, associated with a broader occurrence of opportunistic and potentially pathogenic taxa. Antimicrobial susceptibility testing showed a high prevalence of multidrug resistance across treatments, with broader resistance spectra observed in Cu-containing exposures, consistent with metal-driven co-selection. In contrast, MPs alone did not systematically increase resistance and, for some antibiotics, showed resistance levels comparable to or lower than controls. Integrated multivariate analyses indicated that intestinal pathology and antimicrobial resistance co-varied along gradients of overall stress severity and stressor type, with Cu acting as the dominant driver and MPs exerting a modulatory, context-dependent influence. Overall, these findings highlight the importance of integrated assessments of gut pathology, microbial composition, and antimicrobial resistance to better understand the ecological and One Health implications of combined microplastic–metal exposure in aquatic systems.

Microplastics doi: 10.3390/microplastics5020078

Authors: Polina Todorova Stephany Toschkova Sevginar Ibryamova Kiril Valkanov Teodora Koynova Darina Ch. Bachvarova Nikolay Natchev Tsveteslava Ignatova-Ivanova

Currently, there are no data and studies from Lake Vaya, Burgas city, on the status and quantity of microplastic (MP) particles in fish. This is the first study on the abundance, morphotype, size, polymer type, and color of MP in Gambusia affinis and Liza saliens from the lake. In this study, we also investigated the distribution of MPs in different parts of the fish. Within each morphological group of MPs, three size classes were recognized: 25–100 µm, 100–200 µm, and 200–500 µm. Microplastics were found in all studied fish tissues except for caviar, but in different proportions of pellets, fibers, and fragments. In our study, fibers were the most isolated, followed by irregularly shaped MPs—fragments. Two types of polymers were found—PET and PA. There are currently no studies in Lake Vaya that assess the risk of ingestion of microplastics for fish health and human health. Research shows that almost all aquatic environments worldwide are at risk of MP contamination. Laboratory and field studies highlighted that fish are particularly susceptible to MP ingestion, although freshwater species have been studied less than marine ones. The results of our study suggest that consumption of fish from Lake Vaya may expose citizens to risk.

Microplastics doi: 10.3390/microplastics5020076

Authors: Mithu Chanda Abul B. M. Baki Jejal Reddy Bathi

Microplastics (MPs) are emerging pollutants of global concern, posing significant ecological and human health risks. They are frequently detected in stormwater systems, with urban runoff serving as a major transport pathway into the environment. Green stormwater infrastructure, particularly bioretention systems (BRSs), offers a promising approach to mitigate these risks by filtering and retaining various contaminants. However, the occurrence of MPs in BRSs and their capacity to retain these pollutants remain largely unexplored in the literature, despite being critical for stormwater management and water quality protection. Therefore, this study attempted to examine the occurrence, vertical distribution, and trapping of MPs within a field-installed BRS, potentially emphasizing their role in reducing microplastic (MP) transport. Therefore, field samples were collected at depths of 2, 12, and 24 inches below the surface and processed in the laboratory for MP detection and quantification. The results revealed an average concentration of 1095 particles per kg of dried sediment, with fragments (microplastics shape) accounting for 78.54% of the total MPs. Although no clear vertical distribution pattern was observed, the initial findings showed that MPs were mostly retained at 24 inches, potentially indicating their transport through the media and the retention capacity of a BRS (surface and middle layer) in capturing microplastics from stormwater environments. However, there is no direct evidence to explain the mechanisms driving the observed concentrations at greater depths. The preliminary findings of this study highlight that the concentrations of different sizes of MPs can vary with soil depth in bioretention media. Integrating a BRS into urban stormwater infrastructure likely provides the dual benefits of improved stormwater management and reduced plastic pollution. This study underscores the importance of optimizing bioretention design and media composition to enhance MP trapping from stormwater.

Microplastics doi: 10.3390/microplastics5020075

Authors: Michael Toni Sturm Mirjam Wielandt Pieter Ronsse Anika Korzin Erika Myers Katrin Schuhen

The accurate quantification of microplastics (MPs) in aquatic environments remains challenging due to the heterogeneous distribution of MPs in different environments, making representative sampling difficult, as well as methodological variabilities in sampling, sample processing, and detection. This study examined measurement fluctuations for MP analysis across four distinct water matrices: wastewater treatment plant (WWTP) effluent, tap water (TW), combined sewer overflow (CSO), and surface water (SW). Two sampling strategies were compared: grab sampling (0.5 L, n = 5) and large-volume filtration using a particle sampling unit (PSU; 100 L, 10 µm mesh, subsampled). Samples were processed through oxidative digestion, stained with fluorescent dye, and analyzed via fluorescence microscopy with automated particle counting. Recovery experiments using polyamide (PA) reference particles (357 ± 60 µm) were conducted to assess method accuracy. PSU sampling demonstrated higher precision (mean R.S.D. 41 ± 17%) compared to grab sampling (mean R.S.D. 64 ± 19%), despite additional variability introduced by subsampling. Recovery rates reached 93 ± 7% for grab samples and 88 ± 23% for PSU samples with complete filter analysis. Statistical modeling revealed that achieving a ±25% margin of error (95% CI) required 21 PSU samples versus 51 grab samples. The quadratic relationship between the margin of error and required sample size underscores the importance of methodological optimization for cost-effective monitoring. These findings provide practical guidance for designing MP monitoring campaigns and demonstrate that fluorescent labeling combined with large-volume sampling offers a reliable approach for MP quantification in diverse aquatic environments.

Microplastics doi: 10.3390/microplastics5020074

Authors: Lubomir Petrov Elina Tsvetanova Almira Georgieva Madlena Andreeva Georgi Pramatarov Georgi Petrov Konstantin Dobrev Albena Alexandrova

Microplastics are emerging environmental contaminants capable of crossing epithelial barriers and circulating systemically, potentially affecting organisms, including humans. This study investigated the hematological and biochemical effects of sub-chronic oral exposure to polystyrene microplastics (PS-MPs) in male Swiss albino mice. Animals received 1 μm PS-MPs in drinking water at 0.01 mg/day for four weeks, followed by a two-week recovery period. Blood samples were collected weekly for hematological and biochemical analysis. PS-MP exposure resulted in an increased number of certain immunocytes after the first week of treatment. The highest values compared with the control group were observed in Week 2, reaching 18.5 ± 4.61 vs. 7.2 ± 1.14; 10.9 ± 2.58 vs. 5.1 ± 1.20; and 5.8 ± 2.35 vs. 2.2 ± 0.69 × 109 cells/L for white blood cells, lymphocytes, and granulocytes, respectively (p < 0.001). A significant increase in platelet count was also observed, becoming evident by Week 6 (725.8 ± 307.96 vs. 470.1 ± 121.87 × 109 cells/L, p < 0.05). The elevated alanine aminotransferase and aspartate aminotransferase activities observed after PS-MP exposure were potentially associated with hepatic pathology, erythrocyte damage, and inflammatory responses. No significant recovery was observed during the period after exposure. These findings demonstrate that sub-chronic oral PS-MP exposure induces inflammatory responses and may disrupt organ function.

Microplastics doi: 10.3390/microplastics5020073

Authors: Verdiana Vellani Karin Schlappa Celine Smrekar Tecla Bentivoglio Serena Anselmi Francesca Provenza Ilaria Ceciarini Alessandra Cincinelli Monia Renzi

Understanding the toxicity of micro- and nanoplastics (MNPs) in aquatic systems, combined with temperature, is essential in order to assess ecological hazard in a multi-stressor environment. This study investigated the biological responses of marine and freshwater organisms of different trophic levels (including primary producers, decomposers, and consumers) exposed to polystyrene (PS) MNPs, tested at varying concentrations and particle sizes under two temperature conditions (control and +2 °C). Overall, differences were observed between trophic levels: Paracentrotus lividus larvae were more sensitive to higher temperatures, Daphnia magna exhibited a non-linear pattern, and microalgae have generally shown low sensitivity to both MNPs and high temperatures. However, the MNPs’ responses were not generally concentration-dependent, with the exception of Dunaliella tertiolecta. The effects recorded at increased temperature generally varied among species, indicating that even a moderate increase in temperature can modulate responses in different organisms. In the marine system, hazard levels increased with temperature, whereas in freshwater, they were higher but temperature-independent. These results highlight the importance of integrated assessment approaches to accurately evaluate the ecological hazard associated with MNPs pollution in the context of climate change.

Microplastics doi: 10.3390/microplastics5020072

Authors: Usama Javed Khalid Bani-Melhem Mohamad Abbas Hssa Alkuwari Anjoud Alsulaiti Fatima Alsharoqi

Microplastic (MP) pollution has emerged as a significant environmental crisis across the Arabian Gulf, driven by rapid urbanization, industrialization, and infrastructure challenges in waste management. Studies indicate that MPs are ubiquitous in nature and are present in different environmental compartments, including coastal waters, sediments, marine biota, and the atmosphere. The region is characterized by high salinity, high UV index, and frequent dust storms that can affect the physical and chemical behavior of plastic debris. A consistent finding across regional studies highlights the fibrous polyethylene (PE) and polypropylene (PP) polymer types as dominant microplastic particles. This prevalence of fibrous MPs highlights the role of secondary microplastics that are derived from the fragmentation of larger plastic items and textile-derived materials as a major contaminant source. Ecological impacts are increasingly observed, with studies reporting MP ingestion in commercially important fish species and the potential for biomagnification into the human food web. However, there exist key knowledge gaps regarding the long-term toxicological impacts on human health. This review synthesizes existing data to improve the understanding of microplastic distribution in Qatar and the Arabian Gulf while highlighting the need for standardized monitoring approaches and appropriate waste management strategies.

Microplastics doi: 10.3390/microplastics5020071

Authors: Oscar Ramsés Ruiz-Varela José Juan García-Sánchez Roberto Narro-García Claudia Georgina Nava-Dino Juan Pablo Flores-De los Ríos Luis Fernando Gaxiola-Orduño Alain Manzo-Martínez María Cristina Maldonado-Orozco

The growing accumulation of microplastics in marine environments demands fast and accurate analytical methods for polymer identification. This study presents a new canonical spectral transformation (CST) strategy designed to extract the most relevant information of Raman spectra and enhance the performance of artificial intelligence (AI) models in the classification of microplastics. Using the Marine Plastic Database (MPDB) as the source of Raman spectra, five supervised models—k-Nearest Neighbor (KNN), Random Forest (RF), Extreme Gradient Boosting (XGBoost), Multilayer Perceptron (MLP), and a one-dimensional Convolutional Neural Network (CNN-1D)—were trained and evaluated under both typical (conventional methodology) and CST workflows using 500 noisy samples per category. The CST consists of representing a Raman spectra in a vector where only the magnitude peaks of the most relevant frequency bands of the spectra are retained and the remaining values are null. This CST minimizes the inclusion of non-target data reaching the AI models. All models achieved higher accuracy with CST, where CNN-1D achieved the most significant performance, increasing accuracy to 0.90. In addition, CNN-1D identified Polystyrene (PS) and Poly(methyl methacrylate) (PMMA) with a score of 100% and 99%, respectively. The results demonstrate that CST effectively enhances spectral feature extraction and can be generalized to other spectroscopic techniques, providing a scalable framework for AI-assisted microplastic identification in seawater samples.

Microplastics doi: 10.3390/microplastics5020070

Authors: Martín Armando Román Vega Apolinar Santamaría-Miranda Juan Pablo Apún Molina Iván Guadalupe Martínez Álvarez Ricardo de Jesús Aguilar Romero Karime Anahí Valdez Chávez Máximo García-Marciano Mauro Espinoza Ortíz Luis Parmenio Suescún Bolívar

The presence of microplastics (<5 mm) has become a major threat to marine ecosystems and the organisms inhabiting them. This issue affects a wide range of animals, including commercially important marine fish, whose ingestion of microplastics can cause mechanical and metabolic damage. This study aimed to characterize the main types of microplastic-like particles ingested by Centropomus viridis, Cynoscion othonopterus, Pomadasys macracanthus, Diapterus peruvianus, Lutjanus colorado, and Scomberomorus sierra, important commercial fish species in northwestern Mexico. Four sampling events were conducted over an annual cycle (November to August) in the lagoon and insular systems of Navachiste and Ohuira, Sinaloa, Mexico (RAMSAR sites 1826 and 2025). A total of 556 individuals were captured, and their stomach contents were analyzed using stereoscopic microscopy. Systematic sediment sampling was also performed at each capture site (El Coloradito, El Caracol, El Huitussi, El Aparecido, El Cerro Cabezón, Topolobampo, El Cerro Partido, and El Tortugo) by examining the upper 30 cm of sediment to ensure representativeness of the particle inventory. Four of the six analyzed species (C. viridis, C. othonopterus, P. macracanthus, and D. peruvianus) contained microplastic-like particles (MP-p), totaling 163 items, with an average ingestion rate of 0.29 items individual−1. The omnivorous species D. peruvianus showed the highest ingestion (0.52 items individual−1; 0.0029 items g−1 wet weight). Five categories of MP-p were distinguished based on morphology and fluorescence; however, their polymeric identity cannot be confirmed without spectroscopic analyses. Sediment results showed that most microplastic-like fragments occurred at site 2025 during autumn, spring, and summer, while levels at site 1826 did not differ significantly. This study provides the first evidence of microplastic contamination in these fish species and in this region of northwestern Mexico.

Microplastics doi: 10.3390/microplastics5020069

Authors: Bertha Moreno-Rodríguez Yodaira Borroto-Penton Luis Alberto Peralta-Pelaez Gustavo Martínez-Castellanos Carolina Peña-Montes Humberto Raymundo González-Moreno

The study of river basins is key to understanding the dynamics of microplastic (MPs) generation, transport, and accumulation in regions where various productive activities converge and waste management is limited. The objective of this study was to characterize economic activities in the Tecolutla River basin, Mexico, to identify risk factors associated with MPs generation and release throughout the production chain. A descriptive applied research study was conducted using a structured questionnaire administered to 19 economic units distributed across seven municipalities in the Tecolutla River basin, Veracruz, Mexico. The instrument allowed for the evaluation of the use of plastic materials in inputs, production processes, final products, and waste management practices. Among the economic units analyzed (n = 19), 94.7% reported the use of polymeric materials, with a predominance of thermoplastics such as polyethylene terephthalate (PET), polyvinyl chloride (PVC), and polypropylene (PP), which have a high potential for secondary fragmentation. Within the tertiary sector, accommodation and food preparation services account for the highest proportion of units with limited separation and recycling practices. Activities in the secondary sector, especially the textile and construction industries, showed a high potential for releasing this pollutant due to the use of synthetic fibers, composite materials, and the absence of retention systems. The results provide a basis for the design of mitigation strategies targeting priority productive sectors at the watershed scale.

Microplastics doi: 10.3390/microplastics5020068

Authors: Denise Marie Delvalle Borrero Carlos Mazariegos-Ortíz Anthony Guardia Diego Vásquez

Microplastic (MP) contamination in freshwater systems has emerged as a growing environmental concern. This study investigated the occurrence and seasonal variability of MPs in surface water, the water column, and sediments at selected sites in Lake Alhajuela, Panama. Lake Alhajuela is an artificial reservoir that supplies water to the Panama Canal lock system and to the cities of Panama and Colón, serving more than 50% of the country’s population. MPs were isolated using two digestion protocols followed by density separation, and fragments and films larger than 1 mm were chemically characterized using FTIR–ATR spectroscopy. Mean MP concentrations were 759 ± 536 MPs L−1 in surface water, 328 ± 140 MPs L−1 in the water column, and 109 ± 87 MPs g−1 in sediments. Statistical analyses revealed no significant differences among sampling sites; however, significant seasonal differences were observed (p < 0.01). Smaller MPs (63–249 µm) were more abundant compared to larger MPs (>250 µm). Fragments and fibers were the most predominant type of MP reported. Our results confirm the presence of MPs in the surface and water column, as well as sediments of the Alhajuela Lake. Further studies are needed to elucidate the fate, sources, transport, and distribution of MPs across Lago Alhajuela as well as to assess the lake’s potential contribution of MPs to Gatun Lake and the Panama Canal system.

Microplastics doi: 10.3390/microplastics5020067

Authors: Moayad Yacoub Bangshuai Han

Terrestrial environments function as major sinks and dynamic sources of microplastics. Land use strongly influences inputs, accumulation, and transport pathways of these contaminants in the environment. Despite the extensive literature, few reviews have compared contamination levels and the potential impacting factors across land uses. To fill this gap, this review synthesizes current knowledge on the origins, occurrence, pathways, and ecological effects of microplastics across diverse land uses. The review revealed multiple interconnected pathways that drive microplastic contamination in terrestrial systems. Abundances are consistently higher in intensively managed croplands, urban areas and industrial vicinities. However, their detection in remote environments underscores the critical role of diffuse inputs and long-range atmospheric transport. Vertically, microplastics are enriched in topsoils, and their concentrations declines with depth. Horizontally, concentration declines with increasing distance from major hotspots like agricultural fields, industrial facilities, and road networks. Ecologically, microplastics alter soil physical properties, modify chemical conditions, and shift microbial community composition and enzyme activities. Furthermore, they stress soil fauna and plants through ingestion, toxicity, and physical blockage, with impacts contingent on polymer type, particle morphology, and concentration. Collectively, this review reveals consistent spatial patterns and widespread adverse ecological impacts, highlighting the clear need for integrated management strategies to mitigate terrestrial microplastic pollution.

Microplastics doi: 10.3390/microplastics5020066

Authors: Javier Lorenzo-Navarro José Salas-Cáceres Modesto Castrillón-Santana May Gómez Alicia Herrera

Microplastics represent an emerging threat to aquatic ecosystems, human health, and coastal aesthetics, with increasing concern about their accumulation on beaches due to ocean currents, wave action, and accidental spills. Despite their environmental impact, current methods for detecting and quantifying microplastics remain largely manual, time-consuming, and spatially limited. In this study, we propose a deep learning-based approach for the semantic segmentation of microplastics on sandy beaches, enabling pixel-level localization of small particles under real-world conditions. Twelve segmentation models were evaluated, including U-Net and its variants (Attention U-Net, ResUNet), as well as state-of-the-art architectures such as LinkNet, PAN, PSPNet, and YOLOv11 with segmentation heads. Models were trained and tested on augmented data patches, and their performance was assessed using Intersection over Union (IoU) and Dice coefficient metrics. LinkNet achieved the best performance with a Dice coefficient of 80% and an IoU of 72.6% on the test set, showing superior capability in segmenting microplastics even in the presence of visual clutter such as debris or sand variation. Qualitative results support the quantitative findings, highlighting the robustness of the model in complex scenes.

Microplastics doi: 10.3390/microplastics5020065

Authors: My Yen Nguyen Ann Vanreusel Xuan Quang Ngo Maaike Vercauteren Jana Asselman Carl Van Colen

Analyzing microplastics in marine organisms is essential for understanding the ecological and toxicological impacts of marine microplastic pollution in coastal food webs. This study investigated microplastic ingestion in three edible invertebrate species commonly found on Vietnamese sandy beaches, wedge clam Donax sp., hermit crabs Pagurus sp., and horn-eyed ghost crabs Ocypode ceratophthalmus, which differ in feeding modes and mobility, using micro-Fourier Transform Infrared spectroscopy (µ-FTIR) with a detection limit of 20 µm. Results showed that all three species ingested microplastics, with ingestion patterns varying according to species-specific traits and habitat-related feeding behaviors. The highly mobile crabs Ocypode ceratophthalmus (omnivore) and Pagurus sp. (scavenger) were found to partially reflect the polymer pollution in their ambient environment. The higher ingestion rate and diversity of polymer types observed in sedentary Donax sp. suggest that this species could serve as a potential bioindicator for microplastic pollution, given its mixed suspension and deposit feeding habits that integrate pollution from both the water column and beach sediments. Overall, these results reveal widespread microplastic ingestion among edible beach fauna, highlighting potential ecological and human health concerns, and emphasizing the need for targeted pollution management and increased public awareness. Advancing our understanding will require larger datasets and controlled experiments to more robustly assess species-specific responses and the likelihood of trophic transfer.

Microplastics doi: 10.3390/microplastics5020064

Authors: Mohtasim Ahmed Ashraf Ali Seddique Mohammed Manik Habiba Akther Mohammad Mohinuzzaman Sharmine Akter Simu Tanver Hossain Md. Sahedul Islam Sk Abu Jahid Md. Muzammel Hossain Paolo Pastorino

Microplastics (MPs) are prevalent in coastal habitats, but their occurrence in highly vulnerable coastal zones and human exposure risk are poorly understood, especially in developing nations like Bangladesh. This inquiry focused on the prevalence and potential hazards of MPs in surface sediment and shallow groundwater samples collected from 12 sites in Cox’s Bazar, Bangladesh, from August to October 2023. Using stereomicroscopy and FTIR, MPs were quantified, with concentrations ranging from 60 to 813.33 MPs/kg in surficial sediment and 3.34 to 36.66 MPs/L in shallow groundwater, with mean values of 294.38 ± 26.61 MPs/kg and 18.91 ± 4.75 MPs/L. The dominant MPs were composed of transparent and white fibers, ranging in size from 0 to 0.5 mm, with HDPE (High-Density Polyethylene) and PP (Polypropylene) identified as the most commonly found polymers. To assess MP exposure in humans and the environment, this investigation used three indices: the polymer hazard index (PHI), the pollutant load index (PLI), and the estimated daily intake (EDI). The findings indicate that children exhibit greater exposure than adults, with observed low contamination levels, alongside a spectrum of toxicity from moderate to extreme. This study enhances understanding of MP contamination in the surficial sediments and shallow groundwater of Bangladesh, highlighting the need for further investigation into ecotoxicology, human health risks, legislation, and related issues.

Microplastics doi: 10.3390/microplastics5020063

Authors: M. Curcic I. Stajcic D. B. Stojanovic B. Hadzic P. Mitric A. Bozic Z. Lazarevic P. Kolarz

Heterogeneous photocatalysis increasingly requires rapid polymer degradation tests relevant to aqueous conditions. In this study, a multi-technique approach was developed to monitor the early-stage photo-oxidation of polyethylene (PE) and polystyrene (PS) microplastics in an aqueous ZnO–TiO2 suspension under combined ultraviolet A and ultraviolet B (UV-A/B) irradiation. The changes were analyzed by ATR-FTIR and Raman spectroscopy, DSC, and gravimetric measurements. For PE, the carbonyl index increased from 0.0189 to 0.1350 after 12 h, mass loss reached 16.98%, and crystallinity decreased from 32.05% to 25.36% after 8 h. The Raman spectra of PE showed band broadening and intensity redistribution, indicating increasing structural disorder. In contrast, PS showed weaker Raman changes, while FTIR revealed a non-monotonic carbonyl-index response, and DSC showed a 2.2 °C increase in Tg after 12 h. Gravimetric analysis also showed measurable mass loss in PS, reaching 18.62% after 12 h. The results demonstrate that the combined use of ATR-FTIR, Raman, DSC, and gravimetry enables reliable distinction between early oxidation, surface modification, and material erosion in photocatalytically treated microplastics.

Microplastics doi: 10.3390/microplastics5020062

Authors: María Pilar Yeste Saltanat Bergaliyeva Miguel Ángel Cauqui Miren P. Cajaraville Marta Sendra

In an accelerated aging experiment involving a wide range of cumulative UV-B radiant exposures (up to approximately 9.46 × 103 J cm−2), the degradation state of microplastics was assessed using SEM, FTIR, Raman spectroscopy, and DSC, and correlated with the cumulative UV-B dose. Sunlight-induced photooxidation is a significant weathering mechanism for microplastics. In this study, high-density polyethylene (HDPE), low-density polyethylene (LDPE), polypropylene (PP), polyvinyl chloride (PVC), nylon, and polystyrene (PS) were exposed to UV-B radiation under controlled dry conditions at two irradiance levels (0.06 and 0.6 mW cm−2), covering cumulative UV-B radiant exposures of up to approximately 9.47 × 103 J cm−2. Degradation was evaluated using SEM, FTIR, Raman spectroscopy, and DSC, and was related to the cumulative UV-B dose (H). The extent and progression of degradation varied significantly among the polymers. Overall, FTIR provided the most sensitive assessment of photooxidative surface changes for HDPE, LDPE, PP, and PS, Raman spectroscopy was most diagnostic for PVC (particularly for dechlorination-related changes), and DSC-derived crystallinity was most informative for nylon. These dose-resolved datasets establish a reproducible reference framework (“degradation library”) to facilitate the comparative assessment of the relative photooxidative aging stage of microplastics under comparable surface UV-driven conditions. Outdoor “sunlight-equivalent” times are reported solely as order-of-magnitude contextualization due to environmental variability.

Microplastics doi: 10.3390/microplastics5020061

Authors: Kexin Li Wanglu Li Yuxin Sun Tongtong Ma Lei Yuan Yanna Rong Xiaoyu Liu Yingchun Fu Xiaoping Yu Xiahong Xu

The escalating use of plastic medical products has made medical microplastics (MMPs) contamination an important health concern for specific populations (e.g., patients undergoing medical interventions) and has rendered it a growing focus in global environmental health research. This review systematically summarizes the release characteristics of MMPs throughout their life cycle from device manufacturing and clinical use to waste disposal, and elucidates human exposure pathways. For the general population, environmental exposure and dietary intake are the dominant exposure sources. In patients, however, invasive procedures and intravenous infusions serve as direct, high-concentration routes, enabling MMPs to enter the bloodstream directly. The article focuses on analyzing the molecular mechanisms underlying multisystem pathological effects induced by MMPs, including cardiovascular injury, respiratory dysfunction, digestive disorders, and reproductive toxicity, which involve key pathways such as oxidative stress, inflammatory responses, apoptosis, and dysregulated autophagy. Regarding existing detection technologies, we compare and evaluate the advantages and limitations of microscopic observation, spectral analysis, and chromatography–mass spectrometry in terms of sensitivity, specificity, and applicability, proposing that integrated technical strategies can significantly improve detection reliability. Finally, the review discusses current challenges and future research directions, including the establishment of standardized risk assessment frameworks, the development of highly sensitive in situ detection technologies, and the exploration of targeted intervention strategies. This work provides a theoretical basis for understanding the health risks of MMPs and offers valuable insights for formulating safety management policies for medical plastics.

Microplastics doi: 10.3390/microplastics5020060

Authors: Can Zeytünlü Ceyhun Akarsu

Marine-related activities contribute significantly to plastic pollution in oceans worldwide, particularly in regions located along international maritime routes such as Türkiye. The Dardanelles serves as a major maritime gateway connecting the Aegean Sea with both the Sea of Marmara and the Black Sea, enabling heavy vessel traffic that subsequently disperses plastic pollutants across large areas of the Mediterranean and beyond. Therefore, for the first time, this study applied material flow analysis to estimate potential sources and pathways of macro- and microplastics from 12 vessel categories, including fishing fleets, merchant ships, cruise liners, and military vessels in the Northern Aegean Sea. This approach provides insight into the range of sources and pathways, highlights priority areas for mitigation, and identifies additional knowledge gaps. Through material flow analysis–based estimations, general macro- and microplastic waste is found to be the largest contributor to marine plastic pollution, with an average of 14,965 tons/year, followed by antifouling particles at 5848.5 tons/year. Overall, this study presents a comprehensive evaluation of vessel-derived plastic pollution in a strategically significant maritime corridor and emphasizes its potential implications for the broader marine eco-system.

Microplastics doi: 10.3390/microplastics5010059

Authors: Angel Morán-Silva Daniela Hernández-López Daniela Zúñiga-Rivera Rafael Chávez-López Sergio Cházaro-Olvera Jesús Montoya-Mendoza

Microplastics impact coral reefs due to their presence in the water column. This is the case of Ingeniero Reef, which borders the Veracruz-Boca del Rio metropolitan area. The objective of this study was to determine the presence of microplastics in the feeding zone adjacent to the corals located on Ingeniero Reef. Two sampling periods were conducted (dry and rainy seasons, 2023), obtaining water samples from the area adjacent to the corals and from the water column, with 20 per sampling period. In the laboratory, the microplastics were separated and classified by type, size, and color. A one-way ANOVA was performed to compare microplastic concentration between the two seasons and average size in corals and the water column, and the Bray–Curtis index was applied. A new technique for obtaining microplastics from coral reefs was developed. The concentration of microplastics varies according to the climatic season, with fibers being the dominant type. The size of microplastics can be a determining factor for corals. The development and application of standardized protocols for studying microplastics will allow for more reliable monitoring programs, yielding comparable results that will enable the assessment of the impact of this emerging pollutant.

Microplastics doi: 10.3390/microplastics5010058

Authors: Alexey A. Polotsky Anna S. Ivanova Anna A. Mercurieva Vladimir P. Toshchevikov José Kenny

Accumulation of microplastics (MPs) in aqueous environments poses a serious ecological problem nowadays. MP particles are able to adsorb pollutants of different kinds and to transport them to living organisms, leading to biotoxicity. Hence, investigation of the adsorption of pollutants of different molecular weights onto MP particles is an important task. We employed the numerical Scheutjens–Fleer self-consistent field method to study (i) the formation of MP particles consisting of homopolymer macromolecules and (ii) the adsorption of pollutant homopolymer chains onto the MP particles. Under poor solvent conditions, the polymer macromolecules were shown to form MPs with a constant density inside the particle and with an interfacial layer at its periphery. The size of the MP particles and the thickness of the interfacial layer were controlled by the solvent quality. MP particles were shown to adsorb pollutant polymer chains from the surrounding liquid due to higher compatibility of the MP particle with the pollutant polymer chains as compared to the solvent. The amount of adsorbed polymer pollutant increased with the increase of its concentration in solution. Softer MP particles were shown to adsorb larger amounts of pollutants due to a broader interfacial layer. The conformational characteristics of the adsorbed polymer chains (trains, loops, and tails) were studied in detail.

Microplastics doi: 10.3390/microplastics5010057

Authors: Marco Papparotto Alessio Fonnesu Jeevan Kishore Reddy Pidapa Paolo Matteotti Luca Fambri

Plastic and microplastic (MP) pollution, along with alien species invasion, are of great concern for natural habitat preservation and human health, and are two important and concomitant likely causes for global biodiversity loss. In the present study, a Seabin, a device for buoyant waste collection in calm waters, was used to also characterize the waste collected in northernmost side of Lake Garda (Italy) in a period of very low anthropogenic pressure, the Winter season of 2024–2025. During the survey, 92.6 g of plastic was collected, i.e., a total of 540 pieces. About 6.9 mg of plastic per m3 of water was found, corresponding to about 0.04 plastic items per m3 and approximately 13 pieces of microplastics per day. Fourier-transform Infrared (FTIR) spectroscopy identification showed that the plastic was composed mainly of polyethylene (PE), polypropylene (PP), and polystyrene (PS). Microorganisms (Diatoms, Bacillariophyta) and microcrack formation with deposits of inorganic matter (mainly Si, Al, O, Ca) were also evidenced by SEM/EDX in all the observed aged MP. Qualitative evaluation of the captured biota highlighted the presence of at least five alien species, including invasive Dikerogammarus villosus. This study describes an easy and cost-effective novel methodology for simultaneously monitoring plastic waste and alien species presence in calm waters, which acts also as a mitigation tool for plastic pollution. The results could be of interest not only to policymakers and scientists, but also for public health and for environmental monitoring.

Microplastics doi: 10.3390/microplastics5010056

Authors: Dulce Estefanía Nicolás-Álvarez Ricardo Aguilar-Garay Jorge Alberto Mendoza-Pérez Jeimy Denisse Santiago-Valentín Eva R. Kozak

Microplastics (MPs) are pervasive in marine ecosystems, yet the molecular mechanisms underlying their sublethal effects on zooplankton remain poorly understood. Here, we integrated transcriptomic screening with sequence homology analysis, structural modeling, and molecular dynamics (MD) simulations to examine protein–polymer interaction behavior in the tropical euphausiid Euphausia distinguenda. Transcriptomic analysis identified three MP-responsive proteins; two isoforms (P1, P2) were consistently downregulated following exposure to polyethylene microspheres, while a third candidate (P3) was upregulated but excluded from modeling due to insufficient homology. Structural models of P1 and P2 were generated using AlphaFold2 (ColabFold implementation) and evaluated through comparative structural analyses. MD simulations (100 ns) were conducted using representative polymer oligomers LDPE used as a polyethylene analog and PET and PC included as contrast polymers, which revealed pronounced protein-specific differences and polymer-dependent modulation of interaction stability. Nonbonded Lennard–Jones and Coulomb interaction energies indicated substantially greater intermolecular contact stabilization for P2 than for P1 across all polymers, with LDPE yielding the lowest mean polymer RMSD for P2, and PET for P1. Together, these results indicate that MP–protein interactions in E. distinguenda are consistent with a dominant role of protein structural coherence and dynamic surface compatibility rather than by static structural complementarity or specific binding affinity and provide a reproducible basis for exploring molecular-scale responses to MP exposure in ecologically relevant zooplankton.

Microplastics doi: 10.3390/microplastics5010055

Authors: Israel Ávila-Lázaro Daniel Gustavo López-Couoh Alejandro Ávila-Ortega José Antonio Azamar-Barrios Germán Giácoman-Vallejos Carlos Alberto Quintal-Franco José Ramón Laines-Canepa María del Carmen Ponce-Caballero

The environmental persistence of post-consumer plastics remains a critical challenge due to their chemical stability, the presence of additives, and prior environmental weathering. This study evaluates the partial biodegradation and chemical transformation of post-consumer low-density polyethylene (LDPE) and expanded polystyrene (EPS) by Tenebrio molitor larvae under uncontrolled environmental conditions. Four diets were tested, including LDPE+S and EPS+S (polymers supplemented with wheat bran), to assess the influence of a co-substrate on larval performance and polymer transformation. Fourier-transform infrared spectroscopy (FTIR) revealed the emergence of oxygen-containing functional groups (–OH and C=O) in the frass, which were absent or negligible in pristine materials, indicating oxidative modification of the polymer matrix. Gel permeation chromatography (GPC) revealed pronounced reductions in number-average molecular weight (Mn) and increased polydispersity for EPS-derived fractions, consistent with heterogeneous chain scission and partial depolymerization. For LDPE, GPC evidenced the formation of THF-soluble, low-molecular-weight polymer-derived fragments, indicating fragmentation despite the inability to quantify pristine LDPE due to its insolubility in the mobile phase. Gas chromatography–mass spectrometry (GC–MS) identified aromatic hydrocarbons, phthalate esters, organosiloxanes, and fatty acid derivatives, reflecting both degradation intermediates and migrated additives from post-consumer plastics. Together, these results provide integrated evidence that Tenebrio molitor can induce chemical transformation of post-consumer LDPE and EPS under non-controlled environmental conditions, offering mechanistic insight into a biologically mediated degradation pathway that is directly relevant to realistic plastic waste scenarios.

Microplastics doi: 10.3390/microplastics5010054

Authors: Zoran Stojanović Dunja Drakulić Jelena Martinović Ana Todorović Filip Veljković Nenad Filipović Ivana Guševac Stojanović

While various disturbances in organisms have been reported following long-term oral exposure to small plastic particles (microplastic particles, MPs), the effects of acute, short-term encounters remain underrepresented in scientific research. In this study, adult male Wistar rats were orally gavaged with MPs of three different sizes (~41 µm, 70 µm, or 106 µm; dose: 35 mg/kg), originating from poly(ethylene terephthalate) (PET) bottles. Twenty-four hours post-exposure, the impact on overall health indicators, including food and water intake, sensorimotor function and clinical signs of toxicity, in addition to serum biochemical markers related to organ function and oxidative stress, were assessed. Although no overt sensorimotor impairments or visible toxicity signs were observed in all MPs-treated groups, several investigated biochemical parameters were significantly altered. Water intake was also modified, whereas reduced food intake occurred only in the group treated with median-sized MPs, suggesting that acute exposure to MPs can lead to early physiological and biochemical responses. The obtained results, compared to the data extracted by using machine-learning (ML) tools and GPT-5 model within the available literature, highlighted the importance of investigating the acute effects of MPs, which may precede or contribute to long-term health consequences.

Microplastics doi: 10.3390/microplastics5010053

Authors: Brian Villafuerte-Campi Beatriz Pernía Éricka Maldonado-Narváez José R. Mora Maritza Guaya-Guaya Maritza Cárdenas-Calle Josué Zambrano Angie Calva-Ruiz Jenny Rosero-Lozano

Background: Microplastics (MPs) are increasingly recognized as emerging contaminants in food products, including edible salt. Their presence raises concerns due to potential health impacts and the lack of regulatory frameworks in many countries, including Ecuador. This study represents the first systematic assessment of the occurrence of MPs in commercial salts marketed in Guayaquil and assesses public awareness and willingness to pay for contaminant-free salt. Methods: A total of 45 salt samples covering marine, table, rock, pink, and blue salt, were collected from supermarkets and local stores in Guayaquil. Microplastics were extracted through filtration and oxidative digestion and characterized morphologically under a stereomicroscope. Polymer composition was confirmed using Fourier-transform infrared spectroscopy (FTIR). Additionally, a digital survey was administered to 435 residents to gauge consumer awareness and perceptions. Results: Microplastics were detected in 100% of the salt samples analyzed. Rock and marine salts showed the highest concentrations (>900 items/Kg). Fibers, particularly blue ones, were the predominant morphology, and FTIR analysis identified polyethylene terephthalate (PET), polyamides, and natural fibers. Survey results indicated that only 51.5% of respondents had prior knowledge of microplastic contamination, but 85.7% expressed willingness to pay more for safer salt, and 95.4% supported regulatory measures.

Microplastics doi: 10.3390/microplastics5010052

Authors: Alberto Zoccali Riccardo Ciapponi Serena Ducoli Stefano Turri Stefania Federici Michela Peroni Eleonora Pasinetti Francesca Malpei

Microplastics (MPs) are a growing environmental concern due to their ubiquitous presence, especially in wastewater treatment plants (WWTPs), where they are transferred and accumulated in sludge and can be reintroduced into the environment through sludge reuse. The persistence of MPs highlights the need for effective and tailored treatment strategies to enhance their removal or management. This study investigates the effects and impacts of ozonation as a pretreatment method for sludge, followed by anaerobic digestion (AD), on low-density polyethylene (LDPE), polypropylene (PP), and polyamide 66 (PA(66)) MPs. Different ozone doses, ranging from 5 to 50 gO3/gMPs, were tested in both deionized water and synthetic sludge. The study evaluated MPs degradation through mass variation measurements, Fourier Transform Infrared spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), and Carbonyl Index (CI) analysis. Results showed that ozonation induced chemical modifications in MPs, increasing CI values and leading to the formation of oxygen-containing functional groups, particularly carbonyls. FTIR analysis confirmed the development of new absorption peaks at 1716 cm−1 and 1710 cm−1 for LDPE and PP, respectively, while PA(66) exhibited a shift in its carbonyl peak from 1739 cm−1 to 1754 cm−1. DSC analysis revealed a reduction in crystallinity for all tested polymers, suggesting increased structural disorder. However, no significant MPs mass reduction was observed, and AD did not further enhance MPs degradation. These findings highlight ozonation as a promising strategy for modifying MPs surface chemistry and potentially increasing their environmental degradability.

Microplastics doi: 10.3390/microplastics5010051

Authors: Soibam Ngasotter K. A. Martin Xavier Midhun M. Nair Sandhiya Venkatesh Tao Kara Rupali Das Soibam Khogen Singh Sanjenbam Bidyasagar Singh George Ninan

Microplastics (MPs) have emerged as a growing environmental and food safety concern, with their presence widely reported in aquatic organisms and seafood. However, their occurrence in traditionally processed and fermented fish products remains unexplored. This study provides the first evidence of MP contamination in ethnic fermented fish products of Northeast India, namely Ngari, Hentak, and Shidal. MPs were analyzed for abundance, size distribution, morphology, color, and polymer composition using microscopic examination and Laser Raman Spectroscopy. The average MP abundance was 16.50 ± 5.18 MPs/g in Ngari, 15.73 ± 4.83 MPs/g in Shidal, and 20.50 ± 3.00 MPs/g in Hentak. Fibers and fragments were the dominant morphotypes across all products, with transparent and black particles occurring most frequently. Polymer characterization revealed polyethylene (PE) and polypropylene (PP) as the predominant polymers, followed by polyamide (PA), polyvinyl chloride (PVC), and polystyrene (PS). Size distribution analysis showed that MPs in the 101–300 µm range were most abundant in Ngari and Shidal, whereas smaller MPs (<50 µm) predominated in Hentak. The use of whole fish, including the gastrointestinal tract and gills, primary sites for MP accumulation, along with non-standardized fermentation practices and atmospheric deposition during retail, likely contributes to contamination. These findings highlight an overlooked route of human exposure to MPs through traditional fermented foods and underscore the need for improved processing practices and mitigation strategies to safeguard food safety and sustainability.

Microplastics doi: 10.3390/microplastics5010050

Authors: Elena-Daniela Pantea Elena Stoica Valentina Coatu Elena Ristea Andreea-Mădălina Ciucă

Synthetic or plastic microfibers (MFs) are an emerging form of microplastic pollution in marine ecosystems, derived from textile degradation and weathering of fishing and aquaculture gear. Despite extensive evidence of MFs in marine organisms, the effects of MFs exposure on mussels remain poorly understood. This study investigated the impact of synthetic MFs on the mussel Mytilus galloprovincialis (Lamarck, 1819) over a semi-chronic time scale of 14 days, using MFs produced by grinding a microfiber cloth. Adult mussels were exposed to three MFs treatments: 8, 40, and 100 MFs/L, reflecting current and future scenarios in the Black Sea. Biomarkers assessed included lysosomal membrane stability (LMS), catalase (CAT), glutathione-S-transferase (GST), and acetylcholinesterase (AChE) activities. Significant lysosomal membrane destabilization (p < 0.05) occurred across all treatments. CAT activity in the digestive gland significantly decreased by 31.2%, 53.3%, and 62.1% at 8, 40, and 100 MFs/L, respectively. GST activity showed inhibition at 8 and 100 MFs/L and stimulation at 40 MFs/L. AChE activity decreased at 8 MFs/L but increased at higher concentrations. These results indicate that even environmentally relevant levels of synthetic MFs can alter cellular stability and enzymatic responses in mussels, suggesting potential ecological risks for marine bivalves.

Microplastics doi: 10.3390/microplastics5010049

Authors: Irina Blinova Aljona Lukjanova Anne Kahru Villem Aruoja Margit Heinlaan

Plastic pollution is a global challenge. Despite plastics being complex chemical mixtures, hazard research has focused on particulate forms and the risks of plastic additives, especially for environmental organisms, remain poorly understood. This is a significant knowledge gap considering ubiquitous organismal exposure to plastics and the associated 16,000+ additives. The aim of this study was to provide ecotoxicological characterization of aqueous eluates of foamed plastic consumer products and propose a test battery for toxicity screening. To achieve this, the hazard of eluates of six randomly selected foamed plastic products was evaluated using aquatic decomposers, autotrophs and heterotrophs (Vibrio fischeri, Raphidocelis subcapitata, Lemna minor, Thamnocephalus platyurus, Heterocypris incongruens, Daphnia magna). Alarmingly, all plastic eluates affected the organisms, though toxicity varied among materials and species. Results showed that short-term contact may underestimate plastic eluate toxicity. To increase the environmental relevance of hazard assessment of foamed plastic eluates, harmonizing leachate preparation, using natural water and avoiding (excessive) filtration of eluates should be considered. OECD/ISO assays with R. subcapitata, H. incongruens and D. magna (96 h) can be recommended as a minimal sensitive battery for effective screening of plastic eluate toxicity.

Microplastics doi: 10.3390/microplastics5010048

Authors: Honorio Patiño-Galván María de la Luz Xochilt Negrete-Rodríguez Dioselina Álvarez-Bernal Marcos Alfonso Lastiri-Hernández Guillermo Antonio Silva-Martínez Fabiola Estefanía Tristán-Flores Aurea Bernardino-Nicanor Leopoldo González-Cruz Eloy Conde-Barajas

In recent years, microplastics (MPs) pollution in agricultural soils has increased markedly, largely due to the improper management of plastic mulch films used to improve crop growing conditions. In this context, the present study evaluated the use of biochar (BC) as a soil amendment for mulch-derived MPs-contaminated soils in a radish (Raphanus sativus L.) crop under greenhouse conditions. A pot experiment was established in soils contaminated with MPs (0.5% w/w) and amended with four BC rates (w/w): 0% (Control), 1% (BC1), 3% (BC3), and 5% (BC5). Soil physicochemical indicators were assessed, together with germination, leaf, and radish bulb growth parameters. The experiment was conducted under greenhouse conditions until the radishes reached commercial maturity. Most of the soil’s physicochemical indicators, such as hydrogen potential (pH), electrical conductivity (EC), water holding capacity (WHC), total organic carbon (TOC), organic matter (OM), total nitrogen (TN), ammonium (N–NH4+) and nitrates (N–NO3−), showed significant differences between treatments (p < 0.05), with the exception of the carbon-nitrogen ratio (C/N), which did not vary significantly (p ≥ 0.05). No significant differences were observed among treatments (p ≥ 0.05) for germination indicators. For leaf traits, dry biomass was significantly lower in BC1 than in the other treatments (p < 0.05). For radish bulb traits, fresh weight was significantly higher in BC3 (p < 0.05) compared with the other treatments. Similarly, total plant fresh weight showed significant differences among treatments, with BC3 exhibiting the highest value (p < 0.05). Overall, the BC3 treatment provided the greatest improvement in radish development in MPs-contaminated soil. However, further research involving different types of MPs, BCs, or other crop species is needed to more comprehensively assess the impact of BC on agricultural soils contaminated with MPs.

Microplastics doi: 10.3390/microplastics5010047

Authors: Rumana Hossain Bikesh Ghinangju Montajar Sarkar Veena Sahajwalla

Tyre and road wear particles (TRWPs) represent one of the most significant yet under-recognised sources of global microplastic pollution, contributing up to 28% of total emissions. Generated from the complex interaction between vehicle tyres and road surfaces, TRWPs are chemically diverse, morphologically heterogeneous, and environmentally persistent, making their detection and management particularly challenging. This review provides a critical synthesis of current knowledge on TRWP generation mechanisms, influenced by tyre composition, road types, and vehicle operation, and their environmental dispersion through air, stormwater runoff, snow removal, and roadside deposition. The chemical and physical complexity of TRWPs—often composed of rubber, bitumen, road paint, and heavy metals—necessitates multifaceted analytical approaches. We synthesise current advancements in TRWP identification techniques, including microscopy, micro-spectroscopy, and thermal desorption methods, while benchmarking their applicability using ISO standards. Furthermore, we develop an integrated framework synthesising current classification schemes, detection strategies, and regional policy responses for TRWP assessment. By identifying analytical and regulatory gaps, this review highlights the need for harmonised methodologies, improved analytical comparability, and coordinated policy interventions to address the environmental and health implications of TRWPs.

Microplastics doi: 10.3390/microplastics5010046

Authors: Kylie Babin-Howard Kara Coffman-Rea Karen E. Samonds

Microplastics (<5 mm) are an increasing concern for environmental and human health, continuously detected in ecosystems worldwide and a variety of human tissues. While health effects remain unclear, experimental studies on microplastic particles have suggested adverse outcomes. Microplastic fibers, which shed from everyday items, are more toxic than particles and twice as prevalent, yet remain understudied. Microplastic studies vary widely and use various extraction techniques, with few validating recovery accuracy. These limited recovery studies primarily examine particles, raising concerns about the true abundance of microfibers. This study establishes baseline recovery rates of polyethylene terephthalate (PET) and polypropylene (PP) microfibers of varying lengths from formalin-fixed human cadaveric lung tissue. Following enzymatic and oxidative digestion, PET microfibers showed a recovery rate of 47%, while 87% of PP microfibers was recovered. Chemical alterations were assessed using laser direct infrared (LDIR) spectroscopy; optical microscopy and scanning electron microscopy (SEM) evaluated physical changes post-digestion. These findings provide insights into microfiber recovery, highlight potential over- and underestimations, and characterize the chemical and physical behavior of fibers within human tissue studies. Establishing accurate recovery methods is essential for advancing microfiber toxicology research and assessing potential health risks.