Ecologies doi: 10.3390/ecologies7030084

Authors: Behnaz Balmaki Lee A. Dyer

Environmental change is reshaping ecological communities, yet conservation efforts often continue to focus on protecting species rather than the interactions that sustain biodiversity. Butterfly–plant interaction networks include well-studied systems that are an important component of biodiversity and can be foundational for examining how ecological relationships respond to climate change, habitat alteration, species introductions, restoration, and conservation management. Most studies of these interactions rely on observational records, plant use data, pollen load evidence, and historical specimens to reconstruct how butterflies use plant communities and how these relationships vary across environmental contexts. However, these studies remain scattered across disparate ecosystems, and network data have not been integrated to explain how butterfly–plant interactions are reorganized under environmental change. Here, we synthesize the literature to evaluate how butterfly–plant interactions are reshaped by environmental change in the United States. The evidence indicates that the most impactful environmental change drivers include: increases in temperature, drought, extreme weather, habitat fragmentation, land use change, species introductions, and conservation interventions. These environmental change parameters alter butterfly–plant interactions through partner shifts, temporal mismatch, reduced plant resource diversity, altered plant availability, weakening of specialized links, and increased reliance on generalist plant species. Strong shifts in butterfly–plant interactions may occur even when butterfly and plant species remain present and even abundant, making interaction loss an early signal of ecological change. A network perspective is useful because it can reveal whether specialized or rare butterfly–plant links persist or whether environmental stress shifts communities toward interactions in which butterflies utilize fewer widely used plant resources or plants are reliant on fewer species of butterflies. By integrating observational evidence, historical specimen-based pollen records, restoration contexts, and network analysis, this review provides an interaction-centered framework for understanding and conserving butterfly–plant relationships in changing environments, with implications for ecological sustainability.

Ecologies doi: 10.3390/ecologies7030083

Authors: Puji Harsono Mercy Bientri Yunindanova Jauhari Syamsiyah Andrean Huda Prasetyo

Water hyacinth has become a major environmental problem in aquatic ecosystems worldwide, including Indonesia, due to its rapid growth and extensive surface coverage. Transforming this invasive biomass into high-quality compost offers a sustainable solution for biomass management and agricultural utilization. However, information regarding the combined effects of decomposer selection and the compost aeration process on compost quality, nutrient transformation, and agronomic performance remains limited. This study was conducted in Rawa Pening, Central Java, Indonesia, to determine how decomposer type and turning regime (specifically the frequency and method of mechanically aerating the compost pile) influence the quality of water hyacinth compost and to identify the most suitable formulation for pak choi (Brassica rapa L.) growing media. The experiment consisted of four sequential stages: composting, compost quality assessment, compost selection, and plant growth bioassay. Three decomposers (EM4™, cow dung, and Stardec™) were evaluated under turning and no-turning conditions using a factorial completely randomized design. Compost quality was assessed based on physicochemical characteristics, nutrient composition, compost yield, and correlation analysis. The best compost treatment was subsequently evaluated in different soil–compost mixtures using ANOVA, PCA, and correlation analysis. The results demonstrated that turning and decomposer selection acted synergistically to determine compost performance. Cow dung consistently produced the highest compost yield, whereas Stardec™ combined with turning generated the most mature compost, characterized by the lowest C/N ratio and the highest nitrogen and phosphorus contents. Plant bioassay confirmed that the optimal growing medium consisted of 50% soil and 50% compost, which maximized vegetative growth by balancing nutrient availability with physical support for root development. No toxicity symptoms were observed, indicating that mature water hyacinth compost is safe for agricultural use. These findings provide practical guidance for selecting decomposers based on compost production objectives and promote the sustainable utilization of invasive water hyacinth.

Ecologies doi: 10.3390/ecologies7030082

Authors: Ivan G. Frolov Alexey A. Grachev Anna Khamchukova Yerzhan Toishibekov

The rapid recovery of wildlife populations can outpace the assumptions of integrated population models and make visually plausible reconstructions difficult to distinguish from diagnostically supported inference. We evaluated this problem for Kazakhstan saiga antelope (Saiga tatarica) using a Bayesian model-ladder sensitivity audit for the Betpak-Dala, Ustyurt, and Ural populations from 1980 to 2025. We fitted a climate-aware, age- and sex-structured baseline model family M0 (climate-aware static-K baseline comparator) and compared it with dynamic carrying-capacity, observation-regime, hierarchical-prior, joint-hierarchical, non-centred, and sampling-optimised development families using predefined-convergence, effective-sample-size, posterior-predictive, and observed-to-latent mismatch gates. The baseline reconstruction captured the broad collapse–recovery dynamics but systematically underpredicted the observed abundance in 2021–2025 across all three populations. The strongest development family M4b R2 (sampling-optimised joint-hierarchical development family) improved the R-hat values and late-series alignment, with 2025 observed-to-latent ratios of approximately 1.77, 1.43, and 1.49 for Betpak-Dala, Ustyurt, and Ural, respectively, but the effective sample sizes remained below the gate of 400. The study therefore provides a transparent diagnostic reconstruction and model-development audit rather than management-ready Bayesian integrated population model (IPM) inference, identifying where the current baseline fails and which structural directions require further validation.

Ecologies doi: 10.3390/ecologies7030081

Authors: Francesco Scarton Mauro Bon Roberto G. Valle

Sandy littorals are highly dynamic coastal ecosystems but remain poorly studied as wintering habitats for waterbirds in Mediterranean regions. We analysed mid-winter International Waterbird Census data collected along the 100 km sandy littoral of the province of Venice, northern Adriatic Sea (Italy), between 1994 and 2022. The dataset included 28 winter censuses, 70 species, and 150,083 individuals. The assemblage was rich but highly uneven: the Yellow-legged Gull Larus michahellis, Dunlin Calidris alpina, Black-headed Gull Chroicocephalus ridibundus, and Great Cormorant Phalacrocorax carbo accounted for most individuals. Total abundance was stable, despite strong interannual variability, whereas species richness increased significantly. Eco-functional guilds showed contrasting trends: benthic feeders and diving piscivores increased moderately, omnivores declined moderately, whereas the trends of the remaining four guilds were classified as uncertain. The Community Temperature Index did not show a significant directional trend, either for the whole assemblage or after excluding dominant species, suggesting that the observed changes cannot be interpreted as progressive thermophilisation. Overall, the Venetian sandy littoral supports a diverse and functionally heterogeneous wintering bird assemblage, whose long-term changes reflect shifts in community structure and the changing use of open-coast, nearshore, and artificial roosting habitats. The results provide a long-term evidence base for identifying conservation-relevant shoreline sectors, protecting feeding and high-tide roosting habitats, regulating disturbance and beach-management practices, and maintaining continued IWC monitoring of open-coast waterbird assemblages.

Ecologies doi: 10.3390/ecologies7030080

Authors: Bayron Alexander Ruiz-Blandon Rosario Marilu Bernaola-Paucar Carlos Emérico Nieto Ramos Veronica Zevallos-Guadalupe Luis Armando Nieto Ramos Nora Rodríguez Cangalaya Rafael Julian Malpartida Yapias Alex Marcos Zevallos-Guadalupe

Early establishment is a critical filter in restoration plantings, especially when seedlings must face seasonal water limitation after outplanting. This study evaluated whether nursery-derived functional trait profiles are associated with early field performance of Pinus devoniana Lindl. seedlings in restoration plantings. Six profile codes were built from contrasting combinations of rooting space, nutrient supply, and water regime. Seedling traits were grouped into structural development, belowground investment, allocation quality, nutritional status, stress expression, and field establishment. Functional scores were calculated from standardized variables, and profile responses were examined through conservative comparisons, descriptive principal component analysis, and trait–performance associations. The large-rooting-space, fertilized, continuously irrigated profile (LFI) showed the most balanced pattern, combining positive structural development, high belowground investment, favorable allocation quality, low stress expression, and the highest survival. The large-rooting-space, fertilized, reduced-irrigation profile (LFR) reached the highest field establishment score, but this response was accompanied by stronger stress expression. The large-rooting-space, non-fertilized, reduced-irrigation profile (LNR) had the highest nutritional status, although this did not translate into superior establishment. Under the evaluated conditions, larger rooting space and coordinated belowground investment, allocation quality, and low stress expression were most consistently associated with better early field performance.

Ecologies doi: 10.3390/ecologies7030079

Authors: Aline Lopes Giuliette Barbora Mano Michelle Gil Guterres-Pazin Sthefanie Gomes Paes Vanessa Campagnoli Ursulino Lilian Cristine Camillo Luana Caroliny Possamai Maria Teresa Fernandez Piedade

Ecological niche modeling (ENM) is widely used to predict species distributions and support biodiversity conservation under environmental change, yet its application to Amazonian wetland plants has not been systematically synthesized. We conducted a systematic review following the PRISMA 2020 guidelines to evaluate methodological approaches, environmental predictors, model performance, and research gaps. Literature searches were performed in Web of Science, Scopus, and Consensus (as a complementary AI-assisted academic search platform), yielding 1789 records, of which 48 met the eligibility criteria. Correlative models predominated, with MaxEnt, Random Forest, and ensemble frameworks being the most frequently applied algorithms. Across studies, integrating hydrological and edaphic predictors consistently improved model performance and ecological realism compared with climate-only approaches. Future climate projections indicated greater vulnerability for habitat-specialist species, whereas western Amazonia and the Andean foothills were repeatedly identified as potential climatic refugia. Major limitations included geographically biased occurrence records, limited high-resolution environmental datasets, and the underrepresentation of several Amazonian wetland ecosystems. Overall, the evidence indicates that reliable ENMs for Amazonian wetlands require integrating climatic, hydrological, and edaphic drivers rather than relying solely on macroclimate. Future research should prioritize geographically representative sampling, improved environmental datasets, transparent workflows, and process-informed modeling to strengthen ecological forecasting and conservation planning under climate and land-use change.

Ecologies doi: 10.3390/ecologies7030078

Authors: Jingchun Sun Canying Zhang Linbing Zhang David Gonçalves Shaoping Kuang Hongsheng Yang

Glyphosate is one of the most widely used herbicides worldwide, and its extensive application has raised increasing concern regarding environmental occurrence, ecological exposure, and potential risks to non-target organisms. Its major degradation product, aminomethylphosphonic acid (AMPA), has also received growing attention because of its persistence, transport behavior, and contribution to long-term contamination profiles. To clarify the development and emerging priorities of this field, we conducted a bibliometric review of glyphosate-related publications indexed in the Web of Science Core Collection from 1974 to 2024. After screening and data cleaning, 7050 articles and reviews were included. Publication output increased markedly over time, with annual publications exceeding 300 after 2019 and reaching a peak of 488 in 2023. The United States ranked first with 1928 publications, accounting for 27.4% of the total output, followed by Brazil and China. Keyword co-occurrence, temporal overlay, and collaboration analyses showed that glyphosate research has shifted from early agronomic topics, including herbicide efficacy, crop selectivity, and resistance management, toward a broader environmental research framework. Three major research fronts were identified: mechanism-oriented ecotoxicology in non-target organisms, environmental fate and transport of glyphosate and AMPA across soil–water–sediment systems, and the development of analytical and sensing technologies for environmental monitoring. The results further indicate that the field is moving from single-compound residue assessment toward integrated contaminant-ecology perspectives linking occurrence, transformation, biological response, exposure assessment, and ecological risk. Future studies should strengthen the integration of long-term environmental monitoring, AMPA-inclusive risk assessment, realistic multi-stressor exposure scenarios, and field-deployable detection technologies. This review provides a quantitative overview of the global research landscape and identifies priority directions for environmental assessment and management of glyphosate and AMPA contamination.

Ecologies doi: 10.3390/ecologies7030077

Authors: Xiaolu He Mengyu Wang Dan Yang

Iron oxides are crucial to soil organic carbon (SOC) sequestration in wetland ecosystems. Whether vegetation drives soil microorganisms to regulate iron–carbon interactions and influence SOC sequestration capacity remains controversial. Thus, this study investigated iron–carbon coupling processes and their microbial drivers in the Caohai alpine freshwater closed wetland, Guizhou Province, China. Using metagenomic sequencing and physicochemical analyses, we compared vegetated areas (Phragmites australis and Scirpus tabernaemontani) with bare flats (BF). Levels of SOC, microbial necromass carbon (MNC), and plant-derived carbon (PC) in vegetated soils were significantly higher than those in BF (p < 0.05). In all study regions, the PC/SOC ratio (PA: 27.89 ± 3.59 %; ST: 24.58 ±1.86 %; BF: 24 ±2.82 %) exceeded the MNC/SOC ratio (PA: 15.58 ± 0.94 %; ST: 15.95 ± 0.73 %; BF: 13.06 ±1.30 %). The PC/SOC and MNC/SOC ratios were higher in vegetated areas than in BF areas. Compared with BF, vegetation enhanced wetland SOC stability and promoted carbon sequestration. Iron-bound organic carbon (Fed–OC) content was substantially greater in vegetated soils (PA:0.34 ± 0.08 g/kg; ST:1.07 ± 0.58 g/kg) than in BF (0.14 ± 0.08 g/kg) (p < 0.05). Based on the Fed–OC/Fed ratio (PA: 22.87 ± 14.00 %; ST: 23.00 ± 13.90%; BF: 5.36 ± 3.40 %), Fed–OC associations in vegetated soils were dominated by stable coprecipitation, whereas unstable adsorption prevailed in BF. In all study regions, the abundances of iron-reducing bacteria (FeRB) and iron-oxidizing bacteria (FeOB) peaked in BF. Several FeRB genera, including Thiobacillus, Intrasporangium, Gallionella, and Nocardioides, were significantly and positively correlated with Fed (p < 0.05). Conversely, Geobacter and Nitrospira exhibited significant negative correlations with Fed–OC and PC (p < 0.05). The FeOB genera—Thioalkalivibrio, Thiohalobacter, and Pseudomonas—were negatively correlated with Fed. Vegetation presence appears to influence microbial metabolic potentials through the provision of rhizodeposits and the modulation of rhizosphere conditions, thereby affecting Fed–OC associations and contributing to SOC sequestration in wetland soils to some extent. These findings provide critical insights into plant–microbe–mineral interactions and a scientific framework for predicting carbon sink dynamics in wetland ecosystems.

Ecologies doi: 10.3390/ecologies7030076

Authors: Teresa R. Freitas Paula S. Oliveira André Fonseca Nathalie Guimarães Joaquim Jesus João A. Santos

Phengaris alcon is one of Europe’s emblematic invertebrate species and is currently classified as Near Threatened on the IUCN Red List. In Portugal, this endangered species occurs mainly in Alvão Natural Park (ANP). This study investigated the effects of microclimatic conditions on the adult P. alcon capture records in the Libânia meadow of the ANP. Adult butterflies were monitored (Mark–release–recapture method) from 2006 to 2014 (except 2012). Microclimatic conditions were modelled (NicheMapR), generating values of precipitation (Prec), maximum (Tmax), minimum (Tmin) and mean temperature (Tmean), solar radiation (SR), relative humidity (RH), soil surface wetness (PCTWET), and wind speed (WS). Growing degree days (GDD), cumulative precipitation (Prec-cum), and seven-day means (d7) for all variables were calculated. Seven microclimatic variables showed strong associations with adult abundance. Higher values of Prec-cum, Tmin-d7 and Prec-d7 were negatively associated with abundance, whereas higher values of SR, RH-d7, Tmean-d7 and PCTWET-d7 were significantly associated with butterfly abundance. More than 50% of individuals were recorded within the following favourable climatic ranges: Prec-cum = 157.9–251.4 mm; SR = 6730.1–8220.4 W/m2; Tmin-d7 = 8.0–12.2 °C; RH-d7 = 71.2–86.7%; Prec-d7 = 0.0–0.8 mm; Tmean-d7 = 13.2–18.0 °C; and PCTWET-d7 = 60.8–92.0%. These findings provide quantitative microclimatic ranges that could guide habitat management and support the long-term persistence of P. alcon under future climate change.

Ecologies doi: 10.3390/ecologies7030075

Authors: Yinghui Zheng Yang Lv Xu Su Yuping Liu Mir Muhammad Nizamani Aftab Ahmad Zhaxi Cairang Jieqiong Lei Xuanlin Gao Kaiyue Wei Xu Feng Ting Lv Yanan Wang

Understanding the spatiotemporal dynamics of dominant desert-steppe species under climate change is critical for sustainable management of arid ecosystems. Sophora alopecuroides L., a perennial drought-tolerant leguminous herb with substantial medicinal and forage value, is an important component of these ecosystems. Based on 137 occurrence records and 10 environmental variables, we applied the MaxEnt model and ArcGIS to simulate suitable habitats and range shifts of S. alopecuroides across multiple periods, including the Last Interglacial (LIG), Last Glacial Maximum (LGM), Mid-Holocene (MH), present, and the 2050s and 2070s under four representative concentration pathways (RCP 2.6, 4.5, 6.0, and 8.5). The model showed high predictive performance, with an area under the receiver operating characteristic curve (AUC) greater than 0.9. Temperature annual range (Bio7), elevation, precipitation of the warmest quarter (Bio18), mean temperature of the driest quarter (Bio9), and annual mean temperature (Bio1) were identified as key environmental drivers, indicating that the distribution of this species is mainly shaped by temperature regimes, seasonal water availability, and topography. Under current conditions, the total suitable habitat accounts for 28.9% of China’s territory, closely matching the known distribution. Since the LIG, the total suitable area has fluctuated slightly, being larger during the LIG and LGM, smaller during the MH, and close to the present level thereafter. The distribution centroid remained within the Jinta Basin of the Hexi Corridor, Gansu Province, with only limited displacement from the LIG to the present, suggesting that this area, together with the Tianshan–Junggar Basin margins and the Alxa Plateau–Helan Mountain region, constitute key topographically and climatically inferred potential refugial areas for S. alopecuroides. Future projections indicate that suitable habitat may expand slightly under low-emission scenarios but contract under medium- to high-emission scenarios, with centroid shifts varying among pathways. These findings highlight the potential importance of such bioclimatically inferred putative refugia in maintaining the distribution of dominant dryland herbs and provide a spatially explicit basis for monitoring, germplasm conservation, and adaptive management of S. alopecuroides under ongoing climate change.

Ecologies doi: 10.3390/ecologies7030074

Authors: Roberto Carmona Daniel Galindo-Espinosa Francisco Omar López-Fuerte

Background: Population sex ratios and age structure are fundamental demographic parameters in shorebird studies, and their accurate estimation depends on unbiased capture methods. Methods: To identify potential capture biases, we evaluated the randomness of mist-net recaptures between sex and age classes of the Western Sandpiper (Calidris mauri) in La Paz Lagoon, Baja California Sur, Mexico. Results: A total of 856 individuals were captured and banded, of which 89 (10.4%) were recaptured. Observed and expected recapture frequencies did not differ significantly between sexes (67 observed vs. 62 expected males; 22 observed vs. 26 expected females), indicating no sex-related bias in recapture probability. In contrast, significant differences were detected between age classes (20 observed vs. 42 expected adults; 69 observed vs. 46 expected juveniles), demonstrating a clear age-related bias. The elapsed time between initial capture and recapture was similar for males and females but differed significantly between age classes: adults were most frequently recaptured after 51–75 days, whereas juveniles were recaptured predominantly after 1–25 days. Conclusions: Juveniles were more likely than adults to be captured and recaptured. The higher capture probability of juveniles may be associated with their greater mobility, resulting from their limited experience and lower social status.

Ecologies doi: 10.3390/ecologies7030073

Authors: Paula Sánchez-Carreto Carlos Lara Guillermo Alejandro Pérez-Flores Jorge Vázquez-Pérez Mercedes Rodríguez-Palma

Ethnobiological gardens conserve both biological and cultural diversity, but their permeable boundaries may expose native wildlife inside the garden to chronic domestic carnivore activity. In this single-garden case study, we compare small mammal assemblages between the Jardín Etnobiológico Tlaxcallan (JET), a periurban ethnobiological garden in Tlaxcala, Mexico, and its adjacent, heterogeneous Periphery of native vegetation and agricultural matrix, using live traps, camera traps, and direct observations over nine months. Across 162 detections of 9 species, the Periphery consistently showed higher richness and diversity than the JET at every Hill number order and the two assemblages were compositionally distinct (Bray–Curtis = 0.679), with the JET dominated by disturbance-tolerant species and the Periphery additionally harboring habitat-sensitive natives that were largely or entirely absent from the JET. Domestic cats were detected almost exclusively within the JET, while dogs were recorded at both sites. Dog and cat detection rates were both positively associated with species richness in generalized linear mixed models, a pattern that most plausibly reflects spatial co-occurrence between carnivores and disturbance-tolerant species within the JET rather than a facilitative effect of carnivores on richness. Because our design contrasts a single garden with a single Periphery, these results cannot separate the influence of domestic carnivores from co-varying differences in habitat structure, sampling geometry, or human activity. We therefore present the observed compositional turnover as a hypothesis-generating pattern consistent with—but not evidence of—carnivore-associated ecological filtering that warrants testing through replicated, multi-site designs before informing carnivore management in periurban ethnobiological gardens.

Ecologies doi: 10.3390/ecologies7030072

Authors: Achmad Maulana Malik Jamil Soemarno Antariksa Surjono

Tropical riparian ecosystems are among the most threatened biomes on Earth, yet decade-scale, spatially explicit documentation of vegetation loss and landscape fragmentation in community-based ecotourism corridors remains scarce. This study investigates whether agricultural intensification associated with ecotourism development has driven measurable vegetation decline and landscape fragmentation in the Ledok Amprong riparian corridor (Poncokusumo District, Malang Regency, East Java; 8.21 ha, 800–900 m a.s.l.), a mixed-canopy riparian system dominated by Ficus benjamina, Albizia chinensis, and bamboo (Phyllostachys spp.) interspersed with smallholder coffee (Coffea canephora) agroforestry, over the period 2015–2024. We applied multi-temporal Sentinel-2 (10 m) imagery, NDVI analysis, supervised Maximum-Likelihood classification (Overall Accuracy: 89.75%, Kappa: 0.86), and FRAGSTATS-derived landscape metrics to quantify vegetation dynamics and land cover transition. Results show that agricultural land expanded by 157.51% (1.14 to 2.93 ha), while vegetation cover declined by 28.45% (6.27 to 4.49 ha). A strong negative correlation between agricultural expansion and vegetation loss (r = −0.96, p < 0.001) confirmed agriculture as the primary driver of ecosystem degradation. Landscape fragmentation intensified markedly: patch density increased by 49.6%, the connectivity index declined by 23.5%, and the fragmentation index increased by 54.3%. These findings establish the first quantitative, decade-scale spatiotemporal baseline for this ecotourism corridor and demonstrate that community-based ecotourism development, without adequate spatial governance, accelerates rather than mitigates riparian degradation—a paradox with broad implications for tropical riparian conservation governance worldwide. Evidence-based management recommendations include 10–30 m dynamic buffer zones, a 2.5 ha targeted restoration programme, and a Payment for Ecosystem Services (PES) mechanism, offering a transferable framework for sustainable riparian ecotourism management across rapidly urbanizing tropical watersheds.

Ecologies doi: 10.3390/ecologies7030071

Authors: Abdessadeq Boudjaj Laila Rhazi Jamie Kneitel Mouhssine Rhazi Said Moukrim Mohammed El Madihi Mohamed Ben Bammou Imane Wahby Jorge García Girón Dan Cogălniceanu Patrick Grillas

Increased herbicide use in agriculture is a major threat to adjacent aquatic and wetland ecosystems. In Morocco, the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) poses a risk to temporary ponds found in agricultural landscapes. This study used mesocosm experiments to assess the effects of 2,4-D concentration (0, 0.10, 0.20, and 0.30 mg·L−1) and application timing (early vs. late in the hydrological cycle) on water quality and aquatic plant communities from forest ponds not previously exposed to herbicides. At the highest concentration (0.30 mg·L−1), conductivity increased from 206 to 274 µS and pH from 7.5 to 8.4 under early application. At the same time, species richness and total abundance significantly decreased with increasing 2,4-D concentrations, with the strongest effects observed under early application. Community composition shifted markedly in response to 2,4-D, with annual and submerged species showing greater sensitivity, whereas perennial and emergent species were relatively tolerant. These findings highlight the need to adopt more sustainable agricultural practices to conserve pond biodiversity. Specifically, herbicide applications should be avoided near ponds, at high application rates, and during periods early in the hydroperiod. Lastly, land-use strategies should balance agricultural productivity with the conservation of biodiversity in temporary pond ecosystems.

Ecologies doi: 10.3390/ecologies7030070

Authors: Clementina Araceli Sandy-Pacheco Moisés Martínez-Estrada Ramón Trucíos-Caciano Ricardo David Valdez-Cepeda Hipolito Cortez-Madrigal Jorge Luis Becerra-López Fabián García-González

Vachellia farnesiana is an important shrub in arid and semi-arid ecosystems because it provides resources for diverse insect communities. However, the factors influencing insect assemblages associated with this species under contrasting habitat conditions remain poorly understood. This study evaluated the diversity, composition, and functional groups of insects associated with flowering V. farnesiana at two sites with contrasting tree spatial configurations in Mapimí, Durango, Mexico. Taxonomic diversity was assessed using Hill numbers and sampling coverage, whereas community composition was analyzed using generalized linear latent variable models (GLLVM) and non-metric multidimensional scaling (NMDS). A total of 1461 insects belonging to 6 orders and 33 families were recorded. Although the total abundance was similar between sites, the diversity, community composition, and functional structure differed. Site S1 showed higher richness and diversity (q0 = 33, q1 = 7.62, q2 = 3.85) than S2 (q0 = 16, q1 = 4.23, q2 = 2.87). S1 also supported more parasitoids, predators, and saprophytes, whereas S2 was dominated by phytophagous insects, mainly Aleyrodidae and Thripidae. Sampling coverage was high (S1 = 0.986; S2 = 0.999), indicating adequate representation of both communities. GLLVM detected significant differences in family composition and abundance after accounting for temperature and relative humidity, and NMDS showed partial separation between sites. These findings indicate that habitat characteristics are associated with variation in insect communities linked to V. farnesiana, highlighting its importance as a resource supporting multiple insect functional groups in arid ecosystems.

Ecologies doi: 10.3390/ecologies7030069

Authors: Jatdilok Titioatchasai Komwit Surachat Jaruwan Mayakun

Halimeda-dominated habitats are ecologically significant tropical benthic ecosystems whose structural complexity supports diverse marine organisms, particularly associated microbes. However, the characteristics and ecological roles of these bacterial communities and their connectivity within Halimeda-dominated habitats remain poorly understood. This study examined microbial diversity and composition across four microenvironments of a Halimeda meadow, namely, ambient seawater, sediment, Halimeda thalli, and Holothuria atra feces, using the V3–V4 region of the 16S rRNA gene. A total of 44 phyla, 733 genera, and 827 species were identified, with Proteobacteria, Cyanobacteria, Bacteroidota, Desulfobacterota, and Actinobacteriota dominating across all samples. Microbial diversity and composition differed significantly among microenvironments, with sediment showing the highest species diversity and seawater showing the lowest. Halimeda thalli were dominated by Alphaproteobacteria, particularly Phycisphaerae and Parcubacteria, associated with nutrient cycling on macroalgal surfaces. Sediment was enriched with Desulfobacteria, Actinobacteria, and Chloroflexi. Seawater was characterized by Synechococcus CC9902, a primary producer in tropical waters. H. atra feces were dominated by Bacteroidia and Bdellovibrionota, representing a distinct bacterial community shaped by both gut-associated taxa and microorganisms derived from ingested sediments. Only 6.95% of genera were shared across all microenvironments, and feces and sediment shared the highest overlap (47.28%), suggesting that H. atra acts as a biogeochemical bridge that repackages benthic organic matter without fundamentally altering its microbial identity. The low overlap between Halimeda and seawater, as well as between seawater and both sediment and sea cucumber feces, suggests strong habitat-specific bacterial community assembly, with algal-associated bacterial communities being distinct from pelagic communities and seawater communities differing from benthic and fecal communities.

Ecologies doi: 10.3390/ecologies7030068

Authors: Leslie M. Montes-Carreto Jimena Herandi Espinal-Cárdenas Hanya D. Arellano-Hernández José Antonio Guerrero Esperanza Martinez-Romero

Carnivores harbor gut microbial communities adapted to protein and fat-rich diets. These microbial communities play an important role in amino acid degradation and bile acid metabolism. The bobcat (Lynx rufus) spans diverse North America habitats, yet its gut microbiota across regions remains under characterized. Here, we characterized the fecal microbiota of bobcats from a high-elevation temperate forest in central Mexico (Chichinautzin Biological Corridor) using 16S rRNA gene amplicon sequencing (V3–V4 region). Comparisons with previously published studies were used only to place our findings in the context of the available literature. In total, 632 amplicon sequence variants (ASVs) were recovered from 10 samples. A total of 35 bacterial families and 58 genera were identified. The most abundant genera included Clostridium, Fusobacterium, Bacteroides, and Phocaeicola. Several samples were dominated by genera such as Pseudomonas, Blautia, Ammoniphilus, Alloprevotella, and Hafnia, indicating marked among-sample variability. Functional prediction suggested that pathways related to ABC transporters, purine and pyrimidine metabolism, ribosomal functions, two-component systems, amino acid metabolism, and central carbon metabolism were among the most represented inferred functional categories. These predictions should be regarded as exploratory and hypothesis-generating, providing an overview of the potential metabolic capabilities of the fecal microbiota inferred from 16S rRNA gene data.

Ecologies doi: 10.3390/ecologies7030067

Authors: Ling Nan Yongliu Li Xiangshuai Zhang Qiaorui Ba

Mountain transition zones are highly sensitive to environmental change, yet the nonlinear coupling between topography and hydroclimate in controlling vegetation Net Primary Productivity (NPP) remains insufficiently constrained. Here, we reconstructed a 2003–2025 annual NPP time series for the West Qinling Mountains using a Carnegie–Ames–Stanford Approach (CASA)-based workflow that integrated Moderate Resolution Imaging Spectroradiometer (MODIS) vegetation products, fifth-generation European Centre for Medium-Range Weather Forecasts reanalysis for land (ERA5-Land) meteorological data, Shuttle Radar Topography Mission (SRTM) topography, and an Aridity Index (AI) dataset. Product-based validation against the annual MOD17A3HGF dataset indicated strong agreement, with a 23-year mean spatial Spearman correlation of 0.823 and a mean annual Pearson correlation of 0.773. The reconstructed dataset showed that 98.50% of the study area experienced increasing NPP, including 50.26% with significant increases, and the domain-wide mean Sen slope reached approximately 3.08 g C m−2 yr−1. Factor detection further showed that radiation (q = 0.253), elevation (q = 0.252), and temperature (q = 0.249) were the dominant single controls, whereas Aridity–Temperature (q = 0.367) and Elevation–Aridity (q = 0.367) represented the strongest interactions. The concentration of the strongest gains in gentle-slope and moderate-aridity settings suggests that vegetation recovery is maximized where topographic buffering and water-energy balance are jointly optimized. These results strengthen the interpretation of NPP dynamics in mountainous climate-transition environments and provide a basis for spatially targeted ecological restoration, regional carbon-budget assessment, and climate adaptation planning.

Ecologies doi: 10.3390/ecologies7030066

Authors: Sima Sohrabi Javid Gherekhloo Antonia M. Rojano-Delgado Hadi Nekahi Mohammad Taheri Rafael De Prado José Ramón Arévalo

Managing invasive alien species (IAS) is a major challenge for conserving ecosystems because of their deleterious impacts. This study evaluated the efficacy of five selective herbicides and wheat straw mulch against six invasive alien plant (IAP) species in Iran. Field observations from 2021 to 2025 showed that these species are concentrated in agricultural regions, particularly in summer crops in northern Iran. Five available herbicides (pendimethalin 3 lit h−1, imazethapyr 0.75 lit h−1, nicosulfuron 2 lit h−1, bentazone 2 lit h−1, and 2,4-D+MCPA 1.5 lit h−1) and wheat straw mulch (2 tons ha−1) were used to reduce the growth of six IAP species during summer 2025. Pendimethalin (as a pre-emergence herbicide) was effective (>90%) against all species apart from Ipomoea hederacea (≈60%). While 2,4-D+MCPA (as a post-emergence herbicide) effectively controlled five species, bentazone was effective only against Sida rhombifolia and I. hederacea. Nicosulfuron showed high efficacy (80%) only against I. hederacea. Straw mulch was more effective against Euphorbia nutans but was not effective properly against Ipomoea species. The efficacy of mulch and some herbicides depended on species identity, even within the same genera (Ipomoea and Euphorbia). Our results can help in the successful management of these invasive plants in northern Iran to minimize their impact on yield and quality of crops. As IAPs in environmental areas, this result will also be advantageous.

Ecologies doi: 10.3390/ecologies7030065

Authors: Kooi Chee Ho B. Mabel Manjaji-Matsumoto Liyana Izwin Khalid Jiun Lang Phu Brittany A. Chang Nicolas J. Pilcher

The seas around Sabah host the richest elasmobranch biodiversity in Malaysia. Currently, only 11 shark and ray species are protected under Malaysian legislation, while other species can be legally caught and sold in markets. Many of these species, including those with protected status, are captured as bycatch in commercial trawl fisheries. In Sabah, where trawling is a cornerstone of seafood production, indiscriminate fishing practices prioritize catch volume over gear selectivity. This study focuses on bycatch of Endangered, Threatened, and Protected (ETP) elasmobranchs, which represents an important ecological loss and is rarely retained for legal sale. To determine the spatiotemporal patterns of these captures, custom electronic monitoring (EM) cameras were deployed to capture time- and GPS-stamped imagery of all catch landed on fishing vessel decks. Over a 42-month period, more than 9400 individuals were recorded, of which 34% were identified as Endangered, Threatened, and Protected (ETP) species. The most common ETP shark species were Carcharhinus tjutjot, Rhizoprionodon acutus, and Sphyrna spp., while the most common ETP ray species were Telatrygon biasa, Maculabatis gerrardi/Maculabatis macrura, and Brevitrygon heterura. Spatial analysis and kernel density estimation were used to identify hotspot areas of high bycatch rates. We calculated bycatch per unit effort (BPUE) to quantify the density of ETP shark and ray bycatch across varying fishing intensities, based on the number of individuals caught per trawl towing hours in each district. Seasonal life-stage analyses indicated that neonate and immature individuals comprised a substantial proportion of the catch for several ETP species and occurred across multiple monsoon seasons, highlighting the vulnerability of early life stages to trawl fisheries. Potential mitigation measures, including spatial and temporal management strategies such as time–area closures, are recommended in areas with consistently high bycatch to reduce shark and ray bycatch and protect hotspot areas.

Ecologies doi: 10.3390/ecologies7030064

Authors: Jihane Tellal Laila Rhazi Abdessadeq Boudjaj Issam Ifaadassan Kamal Menzou Fouad Malki Mustapha Moukrim Said Lahssini Rachid Tellal Said Moukrim

Coastal dune ecosystems of the Moroccan Atlantic coast are among the most threatened environments of the western Mediterranean basin, yet the ecological preferences of their constituent psammophilous flora remain poorly documented. Using the ecological profile method, corrected frequencies, species and descriptor entropy, mutual information and ecological barycentres were calculated for three environmental descriptors, distance to the tidal fluctuation zone, topographic position and soil organic matter content, applied to a presence–absence matrix of 53 vascular taxa across 123 plots distributed among three dune facies. Of the total taxa inventoried, 41 were retained as ecologically active. Topography and soil organic matter emerged as the most efficient descriptors, both exceeding the 5% activity threshold. Cross-referencing of ecological groups identified three phytoecological assemblages: characteristic psammophytes of embryonic dunes, species of the primary foredune and species of the enriched backdune. The dominance of intermediate ecological amplitudes and the convergence of introduced species towards organic matter-rich backdune conditions signal chronic anthropogenic disturbance. This study provides a quantitative characterisation of psammophilous species ecological preferences and phytoecological assemblages along the coast–inland gradient of the Haouzia Bay SBEI, constituting an operational reference framework for targeted conservation and restoration management of this threatened coastal site.

Ecologies doi: 10.3390/ecologies7030063

Authors: Ujjwal Layek Arijit Kundu Prakash Karmakar Alokesh Das

Lychee, an entomophilous fruit crop cultivated in tropical and subtropical regions, depends heavily on pollination services for optimal fruit yield and the economic sustainability of farmers. However, information on its pollinator interactions remains limited. This study was conducted over three flowering seasons to document the pollinator assemblage of lychee and examine variation in their activity under different physical conditions, including time of day, panicle height, and light–shade environments. Several insects (here, 47, including many butterflies, bees and flies) were recorded as flower visitors of lychee. The most effective pollinators were Apis cerana, Apis dorsata, Apis florea, Braunsapis mixta, and Tetragonula pagdeni. Pollinator abundance, species richness, diversity, and foraging traits (e.g., flower visitation rate and flower handling time) varied with daytime, inflorescence height, and light availability (light versus shade). Greater abundance, richness, and diversity were documented between 8:00 and 12:00 h, at mid-canopy height (2–6 m), and on well-lit inflorescences. Flower visitation rate was higher under these conditions, whereas flower handling time was lower. This study uncovered the key pollinators of lychee and demonstrated that plant–pollinator interactions vary across different physical conditions. These findings may help to improve pollinator management and enhance pollination services in lychee cultivation.

Ecologies doi: 10.3390/ecologies7030062

Authors: Laura S. Cupitra-Vargas Albeiro Rojas-P Alejandro Lizcano Gabriela Motta-Rojas Oscar Perdomo

Maxillariinae is one of the most diverse Neotropical orchid groups, yet little is known about its reproduction and pollination. We studied Maxillaria carinulata and Maxillaria meridensis on the eastern slope of the Cordillera Oriental in Caquetá, Colombia (2000–2300 m), using pollinator exclusion, controlled pollination, observations, and histochemical tests. Hand pollination was used for manual autogamy, geitonogamy, and xenogamy treatments. Bagged flowers produced no fruit, confirming dependence on pollinators. Apomixis and spontaneous selfing failed, but manual autogamy, geitonogamy, and xenogamy yielded high fruit sets, showing self-compatibility and cross-fertility. In M. carinulata, fruit set reached 93.3%, 86.7%, and 90%; in M. meridensis, 80.0%, 83.3%, and 86.7%. The independent natural fruit-set survey showed low fruit production under field conditions, with 4.7% of monitored flowers forming fruits in M. carinulata and 16.3% in M. meridensis, reflecting inefficient pollen transfer. Both species showed floral scent and osmophore-like activity; M. meridensis also produced lipids, proteins, sugars, and starch in specific tissues. Visitors, mainly Stethobaris weevils, were recorded on M. meridensis, but no pollinarium removal or deposition occurred. These findings highlight strong pollination limitations and support conserving habitats to sustain effective pollinators in Andean populations.

Ecologies doi: 10.3390/ecologies7030061

Authors: Jorge Garate-Quispe Abel Acurio-Lloclla Rembrandt Canahuire-Robles Gaston Coa-Sanchez Gabriel Alarcon-Aguirre

The Brazil nut tree (Bertholletia excelsa Bonpl.) is a non-timber forest product of great ecological and economic importance in the southwestern Amazonian countries. The study aimed to analyze the effects of habitat (natural tree-fall gaps, logged gaps, and field crops) on the survival and early growth of Bertholletia excelsa (Brazil nut) seedlings in the Peruvian Amazon. Seedlings were monitored every 2 months for 1 year to record survival and seedling growth. We used linear mixed models to evaluate the effects of habitat type, time, and their interaction on diameter and height growth. The non-parametric Kaplan–Meier method was used to estimate survival probability. This study showed that the survival of B. excelsa seedlings was significantly higher in canopy gaps than in crop fields. We also revealed the negative effect of canopy cover on the early height growth. Height growth of surviving B. excelsa seedlings was significantly higher in logging gaps than in all other habitats, and in natural tree-fall gaps it was significantly higher than in crop fields, whereas seedlings in logging gaps and crop fields had significantly higher diameter growth than those in natural tree-fall gaps. Our results illuminate the potential of enrichment planting using B. excelsa in logged tropical rainforest in the southeast Peruvian Amazon.

Ecologies doi: 10.3390/ecologies7030060

Authors: Rosana Díaz Silvina Niell María Verónica Cesio Horacio Heinzen

Honey bee (Apis mellifera) colonies are exposed to multiple environmental stressors, including pesticide contamination and landscape changes, which may affect colony dynamics and colony condition. This study evaluated pesticide residue occurrence, landscape quality through the Agroecosystem Apibotanical Interest Index (AABI), and colony demographic structure across five contrasting agricultural systems in Uruguay using a comparative case-study approach. Pesticide residues were analyzed in multiple hive matrices, and residue occurrence was assessed using cumulative hazard quotients (HQ). Colony dynamics were characterized using population variables and the adult-to-brood ratio, associations were evaluated with generalized linear models. Pesticide diversity and cumulative HQ values were higher in intensively managed landscapes, particularly in horticultural and soybean systems, whereas forestry systems showed consistently low values. Residues were detected in all matrices, with greater diversity in wax and bee bread. Colony dynamics varied among landscapes, with more stable patterns in native forest systems. Demographic states based on the adult-to-brood ratio were consistently associated with colony demographic structure. We identified a significant negative statistical association between AABI and the demographic state defined by the adult-to-brood ratio, whereas cumulative HQ showed only marginal effects. Given the observational nature of the study and the multifactorial determinants of colony dynamics, this relationship should be interpreted as an observed pattern within the studied dataset rather than evidence of a direct biological effect of landscape quality on colony adult-to-brood ratio. Overall, the results suggest associations between environmental conditions and colony adult-to-brood ratio.

Ecologies doi: 10.3390/ecologies7020059

Authors: Alexandra D. Solomou Nikolaos Proutsos Panagiotis Michopoulos Athanassios Bourletsikas Panagiotis Lattas

Post-fire rehabilitation structures are widely used in Mediterranean burned landscapes to reduce runoff and sediment transfer, yet their ecological associations with early vegetation recovery remain insufficiently documented. This observational study assessed vascular plant composition, species richness, vegetation cover, plant density, aboveground biomass, and soil properties across log barriers, wattles, and log dams in the burned landscape of Ancient Olympia, western Greece. The study area belongs to the humid climatic class of the United Nations Environment Programme (UNEP) aridity framework based on the Thornthwaite aridity index, providing a comparatively wetter Mediterranean post-fire context. Paired depositional and eroded microsites in operationally restored post-fire areas were monitored in 2022 and 2023. The sampling design comprised nine plots and 18 microsites (n = 9 plots, 18 microsites). Generalized estimating equations (GEE), change-score models, principal component analysis (PCA) and permutational multivariate analysis of variance (PERMANOVA) were performed to examine associations of monitoring year, microsite condition and rehabilitation structure type with soil and vegetation patterns. A total of 27 vascular plant species belonging to 16 families were recorded. The average vegetation cover increased from 39.17 ± 21.44% in 2022 to 75.11 ± 12.90% in 2023. Model-based marginal estimates with 95% confidence intervals indicated a large positive increase in vegetation cover over this period. Further, rapid early recovery was indicated by large increases in species richness, plant density and biomass. Depositional microsites were associated with stronger recovery signals than eroded ones, characterized by a larger increase in vegetation cover, density, biomass and species richness. Among rehabilitation structures, log dams showed the highest cumulative floristic richness and a broader observed floristic spectrum, although the species-level contingency analysis provided only marginal evidence for structure-associated differences in floristic composition. Changes in selected soil properties including total nitrogen (total N), ammonium nitrogen (NH4-N), nitrate nitrogen (NO3-N), pH, electrical conductivity (EC), and exchangeable calcium (Ca), magnesium (Mg), and potassium (K), were detected between 2022 and 2023; the multivariate soil pattern was driven primarily by mineral nitrogen, pH, and EC. These findings suggest that, under operational post-fire restoration conditions, rehabilitation structures are associated not only with erosion-control functions but also with microsite differentiation that may shape early plant establishment and biodiversity recovery in Mediterranean burned landscapes.

Ecologies doi: 10.3390/ecologies7020058

Authors: Shantanu Sharma Jayant Gupta Akshay Jain Debashish Panda Kamlesh K. Maurya Randeep Singh

Understanding dietary patterns and trophic (food) resources partitioning among sympatric mesocarnivores is important for explaining species coexistence through niche differentiation and their roles in ecosystem functioning. We analyzed the feeding ecology and dietary overlap of the golden jackal (Canis aureus) and jungle cat (Felis chaus) using scat analysis in the Kuno–Chambal ravine landscape of central India. We collected a total of 117 scat samples (96 golden jackal, 21 jungle cat) between February 2024 and April 2025 and analyzed them using micro-histological methods to quantify prey composition, frequency of occurrence, and relative biomass. We identified twenty-three prey items from the golden jackal diet. The golden jackal exhibited a generalist feeding strategy characterized by extensive use of livestock carrion (FO > 90%), contributing ~80% of prey biomass, and supplemented by plant resources (FO > 60%) and small prey (FO ~ 40%). The jungle cat consumed sixteen prey items and showed a strong dietary association with small mammals, particularly rodents (FO > 95%) and black-naped hare, which together contributed ~80% of its biomass. Standardized niche breadth was higher for the jungle cat (0.60 ± 0.07) than the golden jackal (0.37 ± 0.03), with a non-significant dietary overlap (Pianka index = 0.45). The high diversity of rodents (n = 11) in the diet of both mesocarnivores suggested their ecological importance in regulating rodent populations and golden jackals, enhancing ecosystem cleaning through their scavenging activity.

Ecologies doi: 10.3390/ecologies7020057

Authors: Kleanthis Patsidis Vassilis Detsis Giorgos D. Kokkoris

A central goal in ecology is to understand whether community assembly follows deterministic rules or is of a stochastic nature. Although species co-occurrence is extensively documented and studied for vertebrates, applying these frameworks to soil Collembola communities in Mediterranean riparian systems provides essential comparative data for community assembly theory. This study examined soil Collembola communities in the Nestos River delta (Greece) across diverse seasons and habitats using thirty-two presence–absence matrices based on abundance data for fifty-four species. These were analyzed using several metrics, each with appropriate randomization algorithms. We studied these metrics across seasons to track community structure changes over time. Additionally, the use of an appropriate multivariate method quantified the influence of soil humidity, while seasonal variations in biomass and diversity were tracked to explore biotic and abiotic influences. In most cases, null hypotheses about the forces structuring these communities could not be rejected, although some instances suggested competitively structured communities. Overall, soil humidity was found to modestly influence community structure, while concordant seasonal trends among biomass and diversity suggest that environmental filtering and biotic interactions shape the observed patterns, with temporal dynamics appearing relatively consistent across habitats within the study year.

Ecologies doi: 10.3390/ecologies7020056

Authors: Zemin Xu Sen Ding Mingqiao Yu Chengxing Xia

The Biological Monitoring Working Party (BMWP) and Average Score Per Taxon (ASPT) indices, which rely on family-level environmental sensitivity values (FESVs), are widely used in freshwater bioassessment. However, regional differences in taxonomic composition often render existing FESV systems incomplete or incompatible, and the influence of rare families remains poorly understood. Using a historical dataset from the temperate Taizi River basin in Northeast China, we developed a regional FESV system for benthic macroinvertebrates. A total of 67 FESVs were established, including 10 families not previously scored in the UK system. These values followed a normal distribution and were ecologically validated using canonical correspondence analysis (CCA). Both BMWP and ASPT indices showed significant correlations with water quality parameters, the Water Quality Index (WQI), and the Habitat Quality Index (HQI). Notably, excluding rare families (occurrence frequency < 1%) did not reduce but slightly enhanced the responsiveness of both indices. CCA identified HQI, conductivity, and ammonia nitrogen as the primary drivers of community composition, and the inferred ecological preferences aligned well with the assigned FESVs. This study provides a robust, regionally adapted framework for family-level bioassessment in temperate East Asian rivers and supports the exclusion of rare taxa to improve cost-effectiveness and index sensitivity.

Ecologies doi: 10.3390/ecologies7020055

Authors: Manolo Rodríguez-Cervantes Antonio Flores-Macías Rodolfo Figueroa-Brito Amanda Kim Rico-Chávez María del Carmen Monroy-Dosta Salvador Alejandro Ventura-Salcedo Vanessa Fernanda Pérez-Castro Mariela González-Rentería Juan Campos-Guillén José Alberto Rodríguez-Morales Karla Elizabeth Mariscal-Ureta Miguel Angel Ramos-López

The yellow mealworm (Tenebrio molitor, Linnaeus) is a cosmopolitan pest of stored grains, causing losses up to 15%. Due to the environmental and health risks of synthetic fumigants, botanical alternatives are needed, but their ecotoxicological assessment is also required. Thus, the aim of this study was to assess the insecticidal, insectistatic, and ecotoxicological effects of Salvia connivens (Epling) dichloromethane extract and to identify its compounds. Insecticidal and insectistatic activities were assessed through the consumption of an artificial diet containing the extract over 30 days. Ecotoxicological activity was evaluated through acute toxicity assays on Danio rerio (Hamilton) adults and embryos. The extract showed insecticidal activity against T. molitor achieving 50% mortality at 10,000 ppm (LC50 = 9367.19 ppm). Additionally, at 10,000 ppm larval weight gain was reduced by 53.37% at 30 days compared to the control. Ecotoxicological assays revealed slight toxicity toward D. rerio adults (LC50 = 84.27 ppm) and embryos (LC50 = 32.60 ppm). GC-MS analysis identified hexadecanoic acid (7.08%), 1-(2-methoxyphenyl)-2,5-dihydro-1H-pyrrole-2,5-dione (6.30%), cis-9-octadecenoic acid (3.91%), β-sitosterol (3.05%), and eicosane (3.00%) as the major constituents according to the chromatographic method used. These findings suggest that S. connivens dichloromethane extract is a potential botanical product for T. molitor management.

Ecologies doi: 10.3390/ecologies7020054

Authors: Wondimu Ersino Ayano Ahmed Awad Zsolt Végvári Tamás Székely

The African jacana (Actophilornis africanus, family Jacanidae) is a sex-role-reversed waterbird inhabiting tropical and subtropical open wetlands across Africa. Identifying environmental factors that influence nest site distribution and suitable nesting habitats is crucial for protecting species and habitat management; however, studies addressing these factors remain limited for this species. This study aimed to map suitable nesting habitats and identify the main environmental variables influencing the nest site distribution of the African jacana in Lake Hawassa, Ethiopia. We mapped nesting habitat suitability using a Maximum Entropy (MaxEnt) model and ArcGIS Pro 3.2.2 based on 78 field-collected nest data points and ten environmental variables. The model predicted a total suitable nesting habitat area of 1.25 km2 with high accuracy (AUC = 0.90, CBI = 0.8, and omission rate = 0.22), representing about 1.2% of Lake Hawassa’s total area. In general, suitable nesting habitats were relatively small, fragmented, and mainly clustered along the southeastern shore of Lake Hawassa. The mean precipitation and temperature during the breeding season, slope, elevation, and distance to shoreline were the main predictors of the African jacana’s nesting habitat suitability. The results of the present study provide valuable insights into local wetland management and the conservation of African jacanas and other breeding waterbirds in Lake Hawassa. Moreover, this study establishes baseline information for assessing how future climate and habitat changes may influence breeding habitat suitability in similar Ethiopian wetlands. The present study was limited to nesting habitat suitability based on limited environmental factors and did not include nest success, pollution parameters or predation; future studies incorporating these factors may improve ecological interpretation.

Ecologies doi: 10.3390/ecologies7020053

Authors: Peter Turis Alena Sliacka Konôpková Daniel Kurjak

Information on factors influencing fitness, flowering, and fruit production in Cyclamen species remains limited. This study evaluated these traits throughout the growing season in three populations of Cyclamen purpurascens subsp. immaculatum under contrasting environments: a young beech forest (half-shade), an old beech forest (shade), and an open meadow. Because this protected endemic taxon of the Western Carpathians (Veľká Fatra and Starohorské vrchy Mountains, Slovakia) cannot be sampled destructively, we used in situ observations and chlorophyll a fluorescence measurements. We found seasonal variation in photosystem II (PSII) efficiency and high environmentally driven inter-individual variability. Plants in the meadow exhibited significantly lower values of Fv/Fm, Fm/F0, and Area parameters, likely reflecting combined microclimatic stress. These plants also produced significantly fewer flowers, suggesting reduced resource availability for reproduction. In contrast, no significant differences in fluorescence parameters were detected between the two forest habitats, which showed similar seasonal dynamics. However, plants in the young forest produced the most flowers and slightly more fruits than those in the old forest or open meadow. Overall, our results suggest that meadow habitats may be less suitable for this taxon, while old-forest environments are tolerated but may reduce reproductive output. Semi-shaded forest habitats may be important for long-term persistence of this taxon.

Ecologies doi: 10.3390/ecologies7020052

Authors: Jan Winkler

The reconstruction of the Stuttgart Hauptbahnhof railway junction, known as Stuttgart 21, is a very large and long-term infrastructure project. The gradual extension of the project implementation creates a specific time period during which atypical vegetation management in the trackbeds takes place. The vegetation of the trackbeds of the current station includes a total of 68 plant taxa, with Erigeron bonariensis L., Geum urbanum L. and Senecio inaequidens DC being significantly represented, for example. The limited level of disturbance within this “time window” creates favorable conditions in particular for the development of woody plants and lianas, such as Acer campestre L., Acer pseudoplatanus L., Ailanthus altissima (Mill.) Swingle, Clematis vitalba L., Ficus carica L., Hedera helix L. and Sambucus nigra L. The detected spectrum of plant taxa also indicates the formation of a diverse mosaic of microhabitats, which allows the coexistence of species with different ecological requirements. The assessed railway lines also provide space for the occurrence of non-native species, many of which are capable of effective wind dispersal and can subsequently colonize surrounding urban areas. Habitats with a time window of limited vegetation management may represent a poorly described factor influencing the spread of some taxa in the technosphere. The knowledge gained may contribute to a better understanding of the population dynamics of individual taxa and their potential for further spread.

Ecologies doi: 10.3390/ecologies7020051

Authors: Sive Tokozwayo Azile Dumani Monde Rapiya Wandile Mashece Ayanda Kwaza Siza Mthi Lwando Royimani

Sorghum halepense is widely recognised as one of the most aggressive invasive perennial grasses affecting agricultural ecosystems worldwide. This systematic review synthesises existing scientific evidence on the ecological invasion dynamics, origin, distribution patterns, impacts on both biodiversity and livestock, and management strategies. A systematic literature review approach was employed to identify and evaluate peer-reviewed and grey literature. Relevant studies were retrieved from major scientific databases, including Google Scholar, PubMed, and ResearchGate, using predefined search terms related to S. halepense, invasion, impact on native plants and livestock, and possible control measures. Articles were screened based on relevance, methodological quality, and thematic alignment with the objectives of the review. The results showed that Johnsongrass is making a gradual invasion in South Africa through seed production and rhizome systems. Sorghum halepense alters native species composition, subsequently reduces biodiversity, and outcompetes native species. Although it may provide forage under certain conditions, its accumulation of cyanogenic compounds and nitrates poses serious poisoning risks to herbivores. Management strategies such as mechanical, burning, and chemical methods vary in terms of effectiveness. Some of these measures are influenced by the genetic make-up of the plant, costs associated with each control measure and other environmental factors. This review highlights the need for integrated management approaches that balance invasive weed control with sustainable forage production. This review emphasises the importance of adopting integrated management strategies that effectively control both seed production and underground stems. Future research should prioritise climate-responsive management approaches, improved understanding of invasion ecology, and the development of cost-effective control measures. Bringing together policy makers and specialists in weed science, natural conservation science, and animal health will be essential for reaching consensus on the actions required to curb the expansion and reduce the economic losses associated with the abundance of Sorghum halepense in our ecosystems.

Ecologies doi: 10.3390/ecologies7020050

Authors: Nicolas J. Pilcher Cambria Davies Eleanor Bowen Sultan Abdullah Alturki Tariq Alqahtani Khalid Imam Modar Al Sulaimani Collin T. Williams Carlos M. Duarte Mohammed Ali Qurban

Effective management and conservation of sea turtles is often constrained by a lack of knowledge of at-sea distribution and abundance. While abundance estimates of nesting females are typically well-documented on nesting beaches, counting sea turtles at sea presents challenges due to their widespread distribution and cryptic habits. Given nesting beaches only document adult females, at-sea data are also more informative of greater population demographics. To estimate the abundance and density of green sea turtles (Chelonia mydas) in the Red Sea waters of Saudi Arabia we conducted strip transect aerial surveys in four survey zones that spanned ~66% of shallow water habitats (<200 m depth), within which we counted sea turtles, and also other species such as dugongs and other marine mammals, sharks, and rays. Corresponding abundance estimates were modelled to account for perception bias (whether a surveyor saw a turtle that was available) and detection bias (whether a turtle was available to be seen). Our results suggest an abundance of ~201,427 green sea turtles potentially present between the 200 m bathymetric contour and the Saudi Arabian shore. However, there was a statistically significant relationship between turtle location and proximity to coral reefs, with over 90% of turtles found within 3500 m of coral reef structures (whether coastal fringing reefs, barrier reefs or atolls), and therefore it would be inappropriate to use an estimate assuming equal distribution. Adjusting for this buffer area we estimated ~95,000 turtles (95% CI: 64,000–142,000) within the proximity of reef structures. These findings represent the first abundance estimates of green turtles in the Red Sea. Repeated over time, surveys such as these can identify changes in population structure, distribution and abundance, and inform conservation and management agencies.

Ecologies doi: 10.3390/ecologies7020049

Authors: Wendell V. de Carvalho Claire Fortunel Cristini da S. M. Fonseca André F. C. Silva Grazielle S. Teodoro Thaisa S. Michelan Ely S. C. Gurgel

In transitional tropical ecosystems such as the Amazonia–Cerrado ecotone, dominant tree species experience strong environmental heterogeneity, requiring coordinated functional strategies to cope with drought, nutrient limitation, and disturbance. However, how these species integrate leaf morphoanatomical traits and wood density to persist in such environments remains poorly understood. We assessed the coordination among leaf anatomical and morphological traits and their relationship with wood density in five dominant tree species across three savanna park sites in the Amazonia–Cerrado transition. Morphological traits included leaf thickness, specific leaf area, leaf dry matter content, and wood density, alongside 17 anatomical leaf traits. We analyzed inter- and intraspecific variation and covariation patterns to identify trait-based ecological strategies along the acquisitive–conservative spectrum. We found strong coordination among traits related to protection (e.g., cuticle thickness and trichomes) and resource use, as well as clear alignment between leaf and wood traits. Species identity explained most trait variation, although leaf thickness showed notable intraspecific plasticity. Species with conservative traits exhibited thicker leaves and higher wood density, whereas species with acquisitive strategy showed higher specific leaf area and lower leaf dry matter content. Overall, trait coordination reflects integrated ecological strategies shaped by environmental heterogeneity, highlighting the role of multi-trait syndromes in driving functional adaptation in ecotonal systems.

Ecologies doi: 10.3390/ecologies7020048

Authors: Kailey Schillinger-Brokaw Aquila Flower

The San Juan Islands are one of the few places where native temperate grasslands are found in western Washington State. These ecosystems are important reservoirs of biodiversity and sources of ecosystem services, support many rare and endemic species, and have profound cultural significance to the Coast Salish peoples. These ecologically and culturally valuable ecosystems have become scarce due to the combined pressures of changes in land use, the introduction of non-native invasive species, and the exclusion of fire from the landscape. A lack of historical context and ecological baseline knowledge has made it impossible to fully understand the long-term trends in the extent and distribution of this ecosystem. To address this knowledge gap, we used historical land cover data and multispectral imagery to create a high-resolution, spatially explicit database of grassland extent on the San Juan Islands at multiple time periods since the early years of Euro-American colonization. Our spatial analysis of these data revealed significant decreases in grassland extent between time periods, with an overall 77% net decrease in the extent of non-agricultural grasslands and a loss of 93% of the area of persistent, old-growth grasslands since 1890 across the region. These changes are primarily a result of conversion to agriculture and conifer encroachment or succession to forest. The spatial data and analyses created in this study help to develop the historical baseline of native temperate grasslands on the San Juan Islands, adding to our understanding of the lingering legacy that changes in land use have had on this ecosystem, with the potential to aid in the development of effective conservation and restoration practices.

Ecologies doi: 10.3390/ecologies7020047

Authors: Zhangeldi Kurganbekov Aspondiyar Utebayev Akbota Aitimbetova

The Shymkent–Saryagash–Abay (A-15) international highway is a major Kazakhstan–Uzbekistan freight corridor that runs through the irrigated horticultural belt of the Turkestan Region in South Kazakhstan, where adjacent fields supply vegetables and cucurbits to the regional market. Composite soil samples (n = 18) were taken at six distances (2–300 m) from the road edge across three locations during 2022–2023, along with edible fruits of tomato, cucumber, watermelon, and melon (n = 12) from the adjoining fields. Pb, Zn, and Cd were measured via flame atomic absorption spectrometry after HNO3/H2O2 digestion. Soil concentrations decreased sharply with distance (Pb: 26.3 → 5.98 mg kg−1; Zn: 21.29 → 4.16; Cd: 0.47 → 0.01 mg kg−1), exceeding the national soil MPCs by 1.5–3 times within 2–10 m. Pb and Zn exceeded the Kazakhstani food-safety MPCs in all four crops, and Cd in three of four (tomato, cucumber, and melon). Transfer factors followed the order of Cd (2.90–4.40) > Zn (1.99–3.00) > Pb (0.16–0.30), and the Cd geo-accumulation index ranged from 1.05 to 1.65 at 2–5 m. Adult dietary risk was acceptable (HI = 0.029–0.052; CR < 1.7 × 10−6), yet food-safety exceedances support a precautionary sanitary buffer and combined soil-and-crop monitoring along the corridor.

Ecologies doi: 10.3390/ecologies7020046

Authors: Yasmine Belabbes-Nabi Rachid Bouhadad Nour El Islam Bachari Souaad Smaï

Ectomyelois ceratoniae (Zeller), the date moth, is a major pest of date palm (Phoenix dactylifera L.), responsible for severe post-harvest losses in arid and Mediterranean regions. The Sterile Insect Technique (SIT) is an environmentally friendly control method whose effectiveness depends on selecting irradiation doses that ensure sterility while preserving insect quality. This study evaluated the histopathological effects of 60Co gamma irradiation on the digestive system of fifth-instar larvae of E. ceratoniae. Larvae were exposed to doses of 0 (control), 250, 300, 350, and 450 Gy, and the mesenteron, proctodeum, and Malpighian tubules were analyzed using Mallory’s trichrome staining. Quantitative measurements included epithelial thickness, intestinal stem cell density, Malpighian tubule diameter, and a histological integrity index. Gamma irradiation induced pronounced dose-dependent alterations. These included thinning and disorganization of the intestinal epithelium, a marked reduction in stem cell density, swelling of Malpighian tubules, and a progressive loss of tissue integrity. Severe degeneration and functional collapse of digestive tissues were observed at doses ≥ 350 Gy. The results indicate that 300–350 Gy represents a critical irradiation range inducing irreversible digestive damage compatible with effective sterilization. These findings provide histopathological reference criteria for optimizing dose selection and quality control in SIT programs targeting E. ceratoniae.

Ecologies doi: 10.3390/ecologies7020045

Authors: Sergio Díaz-Infante Carlos Lara Oscar Gonzalez María del Coro Arizmendi

Plant–animal interactions have shaped much of Earth’s biodiversity. Their structure probably results from a combination of neutrality and constraints imposed by species’ traits, such as phenotypes, phenologies, and spatial distributions, which may affect their stability and coevolutionary processes. Understanding how biological communities are structured remains a major challenge in ecology. To explore this, a hummingbird–plant interaction network from a tropical dry forest in Mexico was surveyed monthly over two years to identify the main drivers of species interactions and network structure. By combining data on morphology, phenology, and the abundance of flowers and hummingbirds, we examined whether biological constraints or neutral processes better explain interaction frequencies and how these factors influence network metrics. Our results indicate that a biological constraint—species phenology—is the most important predictor of plant–hummingbird interaction frequencies. Conversely, null models incorporating abundance best projected most network parameters, suggesting that a neutral process is crucial for predicting these metrics. Interaction networks serve as valuable tools for studying ecological processes. Because previous studies have reported contrasting results regarding the drivers of hummingbird–plant interactions, further comparative studies across ecosystems are still needed to evaluate the generality of these mechanisms.

Ecologies doi: 10.3390/ecologies7020044

Authors: Victor V. Aleksanov Cyrill E. Garanin

(1) Pholidoptera griseoaptera (De Geer, 1773) (Orthoptera, Tettigoniidae) is a common and widespread inhabitant of forest edges in Europe and may therefore serve as a suitable model species for understanding past and future changes in forest wildlife. (2) We recorded the presence or absence of the species in 189 forest and forest-edge plots within the Kaluga Region using acoustic observations and pitfall trapping, and analysed the data using logistic regression. (3) Across the region, the main positive factor affecting species presence was the dominance of nemoral herbs in the herb layer. The main negative factors were habitat isolation caused by physical barriers and location within moraine plains formed during the late stage of the Moscow glaciation. The presence of coniferous tree species and spatial autocovariation were also significant factors, although their contributions were relatively small. The abundance of Ph. griseoaptera was higher in forests located within river valleys. Within Kaluga, the long-term persistence of tree vegetation and habitat isolation were the main significant factors affecting species occurrence. The smallest urban habitat occupied by the species covered approximately 13 ha, whereas the total area of unmown patches within this habitat was only about 0.2 ha. (4) Ph. griseoaptera may be used as an indicator of the long-term persistence of broadleaved deciduous (nemoral) forests. Under conditions of high urbanization, however, the species may become threatened.

Ecologies doi: 10.3390/ecologies7020043

Authors: Kristen M. Kaczynski

Dozer lines can play a key role in the containment of a wildfire; they can also result in long-lasting impacts to vegetation and soils. Despite this, their post-fire ecological impacts are understudied. I assessed vegetation recovery associated with 29 dozer lines within five wildfires that burned between 2018 and 2021 across the Klamath Mountain ecoregion in northern California. Sampling occurred between one and five years post-fire along transects beginning within and extending out 30 m from the dozer line. Vegetation recovery was lowest within the dozer line, where average percent cover ranged from 3 to 5% regardless of years post-fire. Non-native species were found across all sites but were not significantly greater in cover within or outside the dozer line compared with native species. Non-native cover was best explained by elevation and canopy cover, where lower-elevation sites with reduced canopy cover had the greatest cover of non-natives. More research should be done to investigate longer temporal trends in non-native cover post-fire near and within dozer lines.

Ecologies doi: 10.3390/ecologies7020042

Authors: Richard Peñaloza Deyvis Cano Rocio Damian Walter Terrel Humberto Bonilla Raul Yaranga

High-Andean grasslands in the Central Andes of Peru are severely degraded by Lepidium meyenii (maca) cultivation, compromising pasture structure and forage availability for sustainable livestock production. A factorial field experiment evaluated restoration timing and pasture-oriented seed mixtures by manipulating resting time after abandonment (0, 1, 2, and 3 years) and restoration treatment (control; Festuca dolichophylla monoculture; full mixture of Dactylis glomerata + Lolium spp. + Trifolium repens + F. dolichophylla; and mixture without F. dolichophylla) across 64 plots. Vegetation was assessed eight months after seeding, and responses were analysed with ordination, PERMANOVA with restricted permutations, PERMDISP, and generalised linear models and mixed-effects models for diversity metrics. Community composition differed significantly among resting times and seed treatments, with resting time explaining the largest proportion of variance (R2 = 0.353), followed by treatment (R2 = 0.236), while the interaction was significant but smaller (R2 = 0.102, p = 0.002). PERMDISP detected significant differences in multivariate dispersion for both Resting Time and Treatment, indicating that compositional differences may reflect both centroid shifts and heterogeneity among groups. Passive recovery and Festuca-only plots showed slower, more variable compositional change, whereas productive mixtures produced clearer, treatment-specific trajectories over time, suggesting possible divergence in community development patterns, rather than providing formal evidence of distinct alternative stable states. Establishment was consistently high for D. glomerata and Lolium spp., supporting rapid ground cover, which may be associated with short-term forage potential, while F. dolichophylla showed chronically low establishment consistent with limited germination performance. The invasive Pennisetum clandestinum was most pronounced under passive recovery and was reduced under seeded mixtures, suggesting a potential competitive suppression effect. Overall, early seeding with productive mixtures appeared to influence community assembly trajectories, while resting time remained the dominant driver of compositional variation, suggesting potential implications for restoration management in maca-degraded landscapes, although outcomes related to sustainable grazing systems were not directly evaluated.

Ecologies doi: 10.3390/ecologies7020041

Authors: Nassima Khammes-Talbi Farid Bounaceur Nora Khammes-el-Homsi Fatima Zohra Bissaad Naceur Benamor Fatine Lasgaa Stéphane Aulagnier

The last native population of Barbary deer (Cervus elaphus barbarus) lives across the northern border of Algeria and Tunisia; its small population size and global-change pressures limit conservation options, and basic habitat requirements, including diet, remain poorly known. In the Akfadou Forest enclosure (Algeria), where concerns have been raised about woodland condition and limited perennial seedling recruitment, we assessed dietary preferences using micro-histological analysis of faecal samples collected across four seasons. A wide variety of plants was ingested; grasses dominated overall, particularly Avena sterilis and Carex spp., while evergreen trees and woody shrubs also contributed substantially, including Cytisus triflorus, Hedera helix, and Cistus salvifolius. The balance between grazing and browsing indicates that the Barbary deer is an intermediate feeder. Diet composition varied seasonally, with more forbs in winter, more grasses in spring, and greater consumption of trees and shrubs in summer and autumn. These results provide baseline information to support enclosure management and to guide conservation actions for this threatened endemic subspecies, and they highlight the value of complementary analyses of food availability and nutritional quality.

Ecologies doi: 10.3390/ecologies7020040

Authors: Marcelo Kovačić Igor Glavičić Alen Soldo Zoran Valić Dejan Paliska

The present research provides the first quantitative comparison of the hidden fish assemblages in Posidonia meadows and neighboring non-Posidonia habitats. The data and samples were collected at sixty sampling points at three locations on the south side of Brač Island in the eastern Adriatic Sea from October 2023 to June 2025. The gradient of a significant increase in fish abundance and average fish species richness in cuboids and the increase in the frequency of occurrence of fish species were observed from habitats inside Posidonia meadows, over the Posidonia meadow edge, to the habitats outside Posidonia meadows. The primary influence on the abundance was the rarity of species from the family Gobiidae within Posidonia habitats. The markedly different species composition between the Posidonia and non-Posidonia habitats was driven by the high species richness of the family Labridae in the Posidonia habitat compared to the high species richness of the family Gobiidae in the non-Posidonia habitats. The Posidonia meadow edge showed overlap with the two other habitat types, sharing a number of species. The sampling protocol developed in this study is suitable for the quantitative assessment of fishes inhabiting hidden Posidonia microhabitats and provides a methodological basis for future research. The current knowledge of fish in Mediterranean Posidonia meadows, as well as the conservation consequences of still limited knowledge, are discussed.

Ecologies doi: 10.3390/ecologies7020039

Authors: Pietro Orlando Line Østergaard Jensen Cino Pertoldi Sussie Pagh

Effective wolf monitoring is more critical than ever for supporting robust population estimates, identifying breeding packs, and anticipating and mitigating attacks on livestock. This study evaluates bioacoustic monitoring to estimate wolf population size and detect packs and pups based on howling activity, using recordings collected from free-ranging wolves in four different study areas in Denmark. It assesses whether howl structure can be used to identify wild individuals and discriminate between current-year pups (aged between four and nine months) and adults. At wild Location 1, we identified two free-ranging individuals from 40 adult howls by quantifying fundamental frequency features and applying linear discriminant and multivariate variance analyses; the individual classification accuracy was 92%. We examined howls recorded from late August 2021 to February 2022 using maximum fundamental frequency. Across months, mixture modelling and principal component analysis consistently resolved two groups in the wild data, and multivariate tests indicated significant separation each month (p < 0.001), consistent with a pup–adult contrast and the expected autumnal decline in pup frequencies as they mature. A focused analysis restricted to adult-range howls also resolved two groups with very strong multivariate separation (p < 0.001), but this pattern is considered exploratory and does not constitute confirmed sex discrimination. Overall, passive bioacoustics is an effective, non-invasive approach for wide-area monitoring, individual-level acoustic identification, and detecting the presence of pups within wolf packs; however, inference about pack composition or sex based solely on acoustic clustering requires further validation.

Ecologies doi: 10.3390/ecologies7020038

Authors: Desislava Stoianova Ivan Tomov

An association between long-term climatic stability and endemism has been suggested, but it has been tested in plants and vertebrates rather than invertebrates. Using high-resolution paleoclimate reconstructions (CHELSA-TraCE21k; 21,000 BP–present), we tested whether non-cave localities of endemic beetles in the western Balkans are non-randomly associated with local climatic stability. For four bioclimatic variables, we quantified temporal variability using three metrics (SD, range, detrended SD) and defined stability islands as cells in the most stable quartile relative to their neighbourhood at three spatial scales (3 × 3, 5 × 5, 9 × 9). We tested whether 578 endemic-locality cells were enriched in stability islands, against elevation-matched null models. Annual mean temperature produced the highest raw frequency of endemic localities in stability islands, but this pattern was not significant after elevation control. In contrast, endemic localities showed a modest but consistent enrichment in annual precipitation stability islands (observed 9.7–10.7% vs. null 7.3–8.5%; p = 0.01–0.03) across neighbourhood sizes. At the 3 × 3 scale, 60 endemic localities fell within precipitation-stability islands; of them, 20 were outside current protected areas—indicating conservation gaps where minor boundary revisions could enable protection of endemic beetles’ habitats in precipitation-stable sites.

Ecologies doi: 10.3390/ecologies7020037

Authors: Matías Hernández-González Henry Cerbone Chris J. Thorogood

Parasitic plants are ecologically important because they can exert a profound influence on the surrounding ecosystem. Yet the ecology and host specificity of most parasitic plant species remain poorly known or undocumented. Cistanche phelypaea is a local and elusive parasitic plant in the Canary Islands. We carried out the first qualitative assessment of this plant’s ecology on the islands by examining 10 subpopulations over a 7-year period. We examined aspects of the plant’s ecology, distribution, and specificity for eight potential host species. Our observations suggest that four species are hosts: Afrosalsola divaricata, Bassia tomentosa, Suaeda vera, and Traganum moquinii, all of which are shrubby Amaranthaceae; however, host specificity varies across the plant’s range. Afrosalsola divaricata was inferred to be the predominant host and was parasitised wherever it co-occurred with the parasite (50% of sites). Cases of inferred parasitism on more than one host species at a given site were rare. Eight of the ten subpopulations occur in areas of high footfall or close to urbanisation; some disturbance, if managed sensitively, appears to favour recruitment and population dynamics. Based on our observations, we suggest that the integration of species distribution models (SDMs) with targeted surveys would be a promising route for scaling up from site-level observations to island-wide inference. We lay the groundwork for practical recommendations informed by such surveys; together with our long-term observations on host range, this offers a template for parasitic plant conservation more broadly.

Ecologies doi: 10.3390/ecologies7020036

Authors: Rouxin Sun Yanghang Chen Yanyan Yang Xiuwu Sun Peng Xiang Chunguang Wang Bingpeng Xing Yanguo Wang

This study investigates the spatiotemporal dynamics of zooplankton communities in the tropical low-latitude Northwestern Pacific Ocean based on field surveys conducted in August 2021 and November 2022. Redundancy analysis identified nitrate, silicate, temperature, and salinity as the primary factors influencing community structure. The distribution of dominant zooplankton groups exhibited close correlations with key environmental gradients, showing distinct habitat preferences corresponding to different hydrographic conditions. Zooplankton abundance in August 2021 was significantly higher than that in November 2022, which is presumably attributed to eddy-induced nutrient upwelling and enhanced primary productivity. Comparisons with adjacent marine regions reveal general consistency in overall zooplankton abundance and community species composition, while the observed seasonal discrepancies are closely associated with local unique hydrographic characteristics. These results highlight the role of nutrient–temperature–salinity interactions in structuring zooplankton communities and underscore their sensitivity to environmental variability. The findings provide a scientific basis for understanding pelagic ecosystem dynamics in oligotrophic waters and for developing management strategies under changing climate and oceanographic conditions.

Ecologies doi: 10.3390/ecologies7020035

Authors: Yu-Qi Liu Jia-Yuan Zhang Mei-Xin Xia Zi-Yu Tian Zhen Wang Guanglan Cao

Wetlands are ecosystems with critical functions. However, the accelerated progression of global urbanization and human activities, including agricultural encroachment, has resulted in a notable decline in wetland areas and the degradation of wetland quality worldwide. Consequently, wetland restoration has become a central focus of wetland research. Plant community characteristics are among the simplest and most frequently used indicators for evaluating wetland restoration progress and are a crucial factor in maintaining the health and stability of wetland ecosystems. Therefore, this study aimed to investigate the plant community characteristics of restored wetlands with different durations of abandonment in the lower Tumen River Basin, which is expected to provide guidance for promoting the restoration of abandoned farmlands in this region. We hypothesize that species diversity decreases with increasing abandonment age, plant community composition converges toward that of natural wetlands over time, and beta diversity declines due to increasing biotic homogenization during succession. We established a chronosequence of abandoned wetlands in the lower Tumen River Basin, with sites abandoned for approximately 5, 15, and 30 years. And we use natural wetlands and paddy fields as references. With natural succession, the dominant plant species in the restored wetlands transitioned from annuals/biennials to perennials. The aboveground biomass initially increased and subsequently decreased. A gradual decline in species diversity was observed along with a further reduction in beta diversity, and the species turnover component consistently exceeded the richness difference component. The pronounced biotic homogenization among communities indicates that achieving a stable state comparable to that of natural wetlands may require considerably more time or may not be attainable solely through natural succession.

Ecologies doi: 10.3390/ecologies7020034

Authors: Jesse R. Wong-Smer Jorge V. Horta-Vega Crystian S. Venegas-Barrera Rogelio Carrera-Treviño Yuriana Gómez-Ortiz Leroy Soria-Díaz

The daily activity patterns of wild animal species are driven by environmental conditions and plant productivity although the degree of dependence varies according to their ecological niche. Bear ecology is intrinsically linked to seasonal vegetative availability. As omnivores with high metabolic demands, these species rely heavily on botanical resources including fruits, seeds, and roots. Consequently, differences in primary productivity across the landscape influence how individuals distribute their circadian activity patterns. The Enhanced Vegetation Index (EVI) is a tool that quantifies the quality and vigor of vegetation. Relating the EVI to activity patterns allows us to understand how vegetation dynamics and conditions influence the use of time at different times of the day. This study analyzes the daily activity pattern of the American black bear (Ursus americanus) in the El Cielo Biosphere Reserve (ECBR) using camera traps and its association with spatial variations in the Enhanced Vegetation Index (EVI). The results show that the daily activity pattern of the American black bear in the ECBR exhibits a diurnal–crepuscular tendency. In areas with high primary productivity and higher temperatures, activity occurs before sunrise and at sunset, with low activity during the rest of the day. In contrast, in areas with less vegetation and lower temperatures, activity occurs throughout the day. This suggests that, in the ECBR, the activity pattern of black bears could be modulated by temperature variations related to changes in vegetation productivity.

Ecologies doi: 10.3390/ecologies7020033

Authors: Thomas E. Marler

Most cycad seeds germinate under the parent plant, and seedlings die before recruitment to the juvenile stage. Decomposition of the senesced organs releases the nutrients to influence nutrient cycling. The aim of this study was to quantify the soil nitrogen that accumulates from seedling turnover. Soil cores were collected beneath male and female trees of four Cycas species in five Philippine habitats from 2019 through 2025, with matching cores collected 5 m from the trees. Five to nine replications were employed depending on the habitat. One seedling was excavated beneath each tree in one location. Total nitrogen concentration was determined by dry combustion in soil and plant tissues, and total nitrogen content was calculated for seedlings. The soils beneath female trees contained more nitrogen than beneath male trees or away from cycad trees in every habitat. The highest nitrogen concentration within seedlings occurred in coralloid roots, but leaflets contained the most nitrogen pool, indicating rapid release of nitrogen during decomposition of the senesced seedling. No differences in rhizosphere nitrogen occurred in a 2017–2025 ex situ experiment using Cycas edentata, where seeds were sown beneath female and male trees. A second 2018–2025 experiment revealed that female trees provisioned with self-seeds did not differ in rhizosphere nitrogen compared with non-kin seeds. Nitrogen fixed by cyanobacteria endosymbionts of cycad seedlings and programmed seedling mortality combine to influence nitrogen cycling in soils beneath female trees over time.

Ecologies doi: 10.3390/ecologies7020032

Authors: Anush Stepanyan Areg Karapetyan Zhanna Fafuryan Gnel Poghosyan Yulay Yanbaev Aleksey Kulagin

The radial growth of the tree stem reflects tree responses to climate change. This study examines the response of Fagus orientalis to more than half a century of climate dynamics in Armenia using a dendrochronological approach. Two forest stands were analyzed: one geographically isolated stand in the arid southern part of the country and one stand in the mesic northern mountainous region, where the main beech forests are distributed. The study period was divided into two phases (1965–1993 and 1994–2023). Climate dynamics were assessed by the months of the biological year, from October of the previous year to the end of September of the current growing season. Substantial warming trends were detected at both stands, except in November, December, and April, and in July in the northern part of Armenia. Between periods, the mean ring width increased from 1.67 mm to 2.14 mm at the northern stand, while decreasing from 1.95 mm to 0.89 mm at the southern stand. Despite climate warming and declining precipitation, some study trees exhibited increased (northern) or stable (both stands) radial growth. Comparison of the two periods revealed pronounced ecological and tree-specific variability in climate–growth relationships, including shifts in correlation strength and sign reversals. These patterns were primarily driven by climate sensitivity rather than age-related effects. The results provide valuable insights for conserving the southern stand and may support assisted migration strategies for F. orientalis toward the southern margin of the F. sylvatica distribution range.

Ecologies doi: 10.3390/ecologies7020031

Authors: Alena I. Eskova Irina V. Isaeva

This review examines the role of sulfate-reducing bacteria in the anaerobic degradation of hydrocarbons in marine sediments, where they contribute to the mineralization of organic matter under anoxic conditions. The metabolic diversity of these microorganisms is described, including their ability to degrade various classes of hydrocarbons such as short-chain (C2–C5), medium-chain (C6–C12), and long-chain (C13–C20+) alkanes, alkenes, and aromatic compounds like naphthalene and phenanthrene. The primary mechanisms involved in the initial activation of these hydrocarbons—fumarate addition and carboxylation—are discussed, along with key enzymes, including alkylsuccinate synthase and benzylsuccinate synthase. Syntrophic interactions are also considered, particularly in which archaea initiate the oxidation of short-chain alkanes (e.g., ethane and butane), with sulfate-reducing bacteria serving as terminal electron acceptors via sulfate reduction. The potential application of these anaerobic processes in bioremediation strategies for oil-contaminated marine sediments is discussed. This microbially mediated degradation may offer a complementary approach to aerobic methods, particularly in oxygen-limited environments. Understanding the activity of sulfate-reducing bacteria activity is relevant to several areas: the development of remediation techniques for anoxic zones, the assessment of methane emissions from marine sediments, the management of microbiologically influenced corrosion, and potential biotechnological applications. Current research directions include the study of syntrophic microbial consortia and the exploration of bioelectrochemical systems.

Ecologies doi: 10.3390/ecologies7020030

Authors: Manuel Lorenzo Nuñez-Piedra Manuel Jesús Cach-Pérez Gabriela Castellanos-Morales Marivel Domínguez-Domínguez Pablo Martínez-Zurimendi

This study analyzed seven foliar traits of Cedrela odorata L. (Meliaceae) in 87 individuals across three germplasm movement zones in Tabasco, Mexico (VIII: humid; XIV: intermediate; XXI: dry) to assess differentiation and climatic relationships. Leaf length, area, petiolule length, leaflet number, rachis length, width, and stomatal density were measured. Univariate tests, canonical correlation analysis, redundancy analysis, and a relative phenotypic plasticity index were applied. Significant morphological differentiation was found: zone XIV exhibited the largest leaves and longest rachises, zone VIII the highest stomatal density, and zone XXI smaller, more subdivided leaves. The first canonical axis (r = 0.846, p < 0.001) associated long and wide leaves with warm, humid conditions, while the second (r = 0.810, p < 0.001) linked stomatal density and width to temperature minimum. Climate explained 55.7% of morphological variation, primarily through water and temperature gradients. High plasticity in leaf area, rachis length, and stomatal density suggests adaptive flexibility, yet consistent inter-zonal differences indicate local adaptation. These results demonstrate a strong correspondence with Tabasco’s germplasm movement zones and providing morphological evidence to support reforestation and germplasm management strategies under variable climatic conditions.

Ecologies doi: 10.3390/ecologies7010029

Authors: Alberto Gianinetti

Living organisms continuously capture and transform free energy to survive and grow. The Maximum Power Principle (MPP) states that life evolves to maximize power—the rate of energy acquisition and conversion into useful forms—within prevailing constraints. Constraints include trophic interactions and competition, and they determine the outcome of the MPP. Biosystems that accumulate free energy faster will prevail transiently, but those that do so in a stable way will dominate in the long run. Accumulation of free energy is often approximately measurable as biomass that is useful to improve adaptation, competition, and ecological stability. Biosystems that allocate energy to long-term stable biomass—such as forests investing in wood—dominate because they secure long-term resource capture, competitive advantage, and resilience. Species and ecosystems represent different scales at which the MPP applies. In the long run, the MPP must align across scales, because a species can achieve stable growth and maximize power (i.e., accumulate free energy/useful biomass) only if the ecosystem the species lives in also maximizes its power. If a species reduces the stability and power of its ecosystem, it undermines its own long-term power-acquisition capability. This feedback harmonizes the MPP across scales over evolutionary times. Humans have evolved peculiar traits that have made them able to remove this control loop, amplifying species-level power. This enabled us to grow into a large population supported by anthropic systems that have strongly reduced the biosphere’s stability and power, resulting in a scale conflict in the MPP. Outlined in this way, the MPP provides a useful framework for understanding evolution, ecosystem development, and anthropogenic impacts.

Ecologies doi: 10.3390/ecologies7010028

Authors: Jovo Pokrajac Tamara Karan-Žnidaršič

Freshwater ostracods have considerable potential as indicators of environmental conditions, yet their ecology remains poorly documented in many large river floodplains of Southeast Europe. This study examines samples collected from ten aquatic habitats located along the Danube floodplain in Serbia’s Banat and Podunavlje regions. Monthly sampling was conducted over a twelve-month period (July 2023–June 2024), with concurrent measurements of water temperature, pH, dissolved oxygen, electrical conductivity, and turbidity. Ostracods were recorded at seven sites, yielding 19 taxa belonging to 13 genera and four families within all three non-marine superfamilies of Podocopida. Eight recorded taxa represent new additions to the Serbian fauna. Species richness was highest in semi-isolated floodplain habitats. Canonical correspondence analysis (CCA) showed that seasonal environmental variation, especially water temperature, turbidity, and conductivity, strongly structured assemblages. Hierarchical cluster analysis (UPGMA) grouped samples primarily by species composition, with seasonality exerting a strong secondary influence. Seasonal patterns revealed pronounced interspecific differences in temporal persistence and ecological tolerance of recorded species. Findings highlight the Danube floodplain’s role as a dispersal corridor, while also revealing that the river itself acts as a partial barrier, restricting faunal exchange to widespread, tolerant species. The results emphasize the importance of habitat heterogeneity and year-round sampling and support the integration of ostracods into long-term floodplain monitoring programs.

Ecologies doi: 10.3390/ecologies7010027

Authors: Ionuț-Ștefan Iorgu Ioan Tăușan Carmenica-Rahela Oloeriu Alexandra-Florina Popa Elena Iulia Iorgu

Urban parks derived from historical forest fragments represent important refugia for biodiversity in rapidly expanding cities. The wood cricket, Nemobius sylvestris, was surprisingly found in a park in the northern part of Bucharest, Romania, an area under exponential residential development. The species was confirmed by calling song analysis and molecularly confirmed through DNA-barcoding. The acoustic analysis revealed substantial geographic variation in the signals of N.sylvestris across its European range, with the Romanian population exhibiting the most distinctive acoustic characteristics. A median joining network was constructed using available COI sequences from public databases, showing moderate genetic variability within European samples. This flightless, woodland-specialist cricket is highly sensitive to habitat fragmentation and its persistence in this urban park demonstrates the conservation value of retaining semi-natural forest structure within city green spaces. Our findings highlight the importance of urban parks as biodiversity refugia, particularly for habitat specialists with limited dispersal abilities. This discovery underscores the need for the integrative conservation management of urban forest remnants, emphasizing the retention of natural structural elements such as leaf litter and heterogeneous canopy cover to support diverse invertebrate communities.

Ecologies doi: 10.3390/ecologies7010026

Authors: Marcus Paulo Alves de Oliveira Ataliba Henrique Fraga Coelho Luís Beethoven Piló Rodrigo Lopes Ferreira

Iron-ore extraction plays a central role in the global economy, but several major mining areas overlap with ecologically unique ferruginous landscapes in the Brazilian Amazon, including caves that harbor endemic and highly specialized invertebrate fauna. Reconciling mineral exploitation with biodiversity conservation requires objective ecological criteria capable of supporting evidence-based decision-making. In this study, we evaluated how cave attributes and surrounding landscape features jointly structure invertebrate communities in pristine ferruginous caves of the Amazon and assessed their relative importance and environmental thresholds. Invertebrates were sampled in 69 iron-ore caves during dry and wet seasons, and 28 environmental variables related to cave morphology, microclimate, trophic resources, lithology, vegetation cover, and external climate were measured in subterranean habitats and adjacent landscapes. Our results demonstrate a clear scale-dependent pattern: cave attributes primarily regulated species richness, troglobitic richness, taxonomic distinctness, and seasonal beta diversity, whereas landscape features exerted stronger control over species composition, including troglobitic assemblages. Threshold analyses identified specific combinations of cave and landscape attributes associated with biologically pristine communities. These findings highlight that assessments of ferruginous cave biodiversity must integrate landscape-scale metrics, be conducted in unaltered environments, and prioritize networks of caves rather than isolated sites. This integrative framework provides robust ecological support for conservation planning and the sustainable management of iron-ore cave systems in the Amazon.

Ecologies doi: 10.3390/ecologies7010025

Authors: Lidia Rodríguez-Arredondo Olivia Millán-Aguilar Miguel Ángel Hurtado-Oliva Marlenne Manzano-Sarabia

Mangrove wetlands in northwestern Mexico have been highlighted due to their ecological relevance and ecosystem services. This study evaluated the mortality and natural regeneration of mangroves located in six coastal lagoons in Sinaloa, considering five plots each (400 m2), during a warm–strong (2015–2016) and cold–weak (2017–2018) El Niño–Southern Oscillation. The highest mean mortality was recorded in Huizache–Caimanero—the southern coastal lagoon—during the second stage (390 stems ha−1; 22% corresponding to logging). While an increasing latitudinal (north–south) mortality trend was observed, differences between sites and stages were not statistically significant. Natural recovery was also observed due to higher abundance of seedlings, e.g., the largest increase from one stage to another was recorded in Santa María–La Reforma. Mortality and seedling regeneration are discussed in this study, particularly in relation to anthropogenic stressors, logging, and climate variability.

Ecologies doi: 10.3390/ecologies7010024

Authors: Attila Haris Zsolt Józan Attila Balázs George Japoshvili György Csóka Anikó Hirka

To investigate the long-term effects of climate change on biological communities, our primary aim was to identify the most reliable indicators among available biodiversity, dominance, and evenness indices. We examined three distinct response types to climate change, represented by three taxonomic groups: Aculeata (Hymenoptera), Syrphidae (Diptera), and nocturnal macrolepidoptera (Lepidoptera). Using faunistic datasets derived from our own 3–5 decades of field surveys, we calculated 12 key indices with the vegan package in R 4.2.1. The robustness of these indices was assessed through 1000-fold bootstrap simulations and pairwise correlation analyses. Our results revealed that the Gini–Simpson, Simpson diversity, McIntosh diversity, and McIntosh evenness indices consistently demonstrated high temporal stability and strong correlations across all three climate response types. Therefore, we recommend these indices as primary climate indicators. In contrast, Chao1 estimates, Margalef Index, Menhinick Index, and the Shannon–Wiener diversity index are suitable only for analyzing specific response patterns. Meanwhile, the Berger–Parker, Buzas–Gibson indices, and Hill numbers showed high variability or limited ecological responsiveness, making them unreliable for tracking climate change impacts. Our findings underscore that selecting biodiversity indices must be tailored to the research question and the characteristics of the ecosystem in order to ensure valid and informative ecological analysis.

Ecologies doi: 10.3390/ecologies7010022

Authors: Murtada Naser Amaal Yasser Antoni Vivó-Pons Burçin Aşkım Gümüş Patricio R. De los Ríos-Escalante

This study assesses the diversity, distribution, and ecological interactions of freshwater gastropod communities across eight sites in southern Iraq, spanning marshes, rivers, and canal systems within the Tigris-Euphrates basin. Diversity indices (Shannon–Wiener H′ and Pielou’s evenness J) were calculated to evaluate community structure, and a revised stacked bar chart of relative abundances revealed widespread dominance by species such as Melanoides tuberculata and Physella acuta, which frequently exceeded 50% of local assemblages. While P. acuta is an established invasive species, M. tuberculata is now considered native or long-established in the region. Species interactions were examined using Pianka’s niche overlap index, and null model testing (999 permutations) revealed only a few statistically significant overlaps (p < 0.05), suggesting that species co-occurrence is shaped more by environmental filtering than direct competition. To directly examine the influence of environmental drivers on species composition, Multiple Factor Analysis (MFA) was performed. MFA revealed patterns of association between dissolved oxygen, salinity, and species assemblages, suggesting these abiotic factors may influence community structure. To our knowledge, this study is the first in Iraq to use null models, constrained ordination, and MFA to investigate community assembly of freshwater gastropods, ultimately producing novel insights regarding the interactions between environmental stressors and aquatic biodiversity patterns. The results of this study highlight the need for long-term ecological monitoring and conservation in marshland habitats important for the resiliency of native species.

Ecologies doi: 10.3390/ecologies7010023

Authors: Diego Portalanza Emily Valle Manuel Cepeda Liliam Garzón Juan Carlos Guevara Diego Arcos Carlos Ortega José Ricardo Macías-Barberán

Mangrove ecosystems are recognized as highly efficient blue-carbon reservoirs, yet their monitoring requires scalable, transparent methods suitable for climate-finance and greenhouse-gas accounting applications. This study quantifies interannual carbon-stock dynamics and derives a carbon-market valuation indicator for Ecuador’s Churute Mangrove Ecological Reserve (2015–2021) using publicly available remote-sensing land-cover products. Annual activity data were derived from Copernicus Global Land Service LC100 (100 m, 2015–2019) and ESA WorldCover (10 m, 2020–2021), harmonized to a common reporting scheme, and combined with IPCC Tier 1 default coefficients for biomass and soil organic carbon in tropical wetlands. Total carbon stocks averaged 1.67 million t C across the period, remaining stable within the internally consistent LC100 phase (2015–2019), with trend statistics treated as descriptive given the short annual series, while a pronounced drop in 2020 primarily reflected methodological discontinuities between products rather than ecological change. Converted to CO2e equivalents (mean 6.1 million t CO2e), illustrative market values fluctuated between USD 18 and 123 million annually, driven predominantly by carbon-price variability. This remote-sensing-based, MRV-aligned approach provides a conservative baseline for protected-area blue-carbon accounting, highlighting the need for homogeneous high-resolution time series to distinguish real dynamics from classification artifacts in future assessments.

Ecologies doi: 10.3390/ecologies7010021

Authors: Feng-Jiau Lin Shu-Hui Chang Cheng-Wei Lin Kuan-Feng Huang Hsiao-Yun Chang Yih-Tsong Ueng

Mangrove ecosystems along Taiwan’s southwest coast have been increasingly stressed by climate change, subsidence, and sea level rise. Between 1897 and 2024, the mean annual temperature rose by 2.0 °C, and rainfall declined by 56.5 mm. Severe subsidence occurred in Taixi Township, Yunlin County (−283.0 cm, 1975–2023), where the gray/white mangrove (Avicennia marina) exhibited reduced growth and mortality. Long-term mangrove area (MA) was reconstructed using quadratic polynomials: Tougang Ditch, MATG(t) = −0.0084(t − 21.0)2 + 2.8 peaking in 1995 (R2 = 0.7274), and Budai Lagoon, MABD(t) = −0.0468(t − 12.3)2 + 26.1 peaking in 1986 (R2 = 0.782). Both sites yielded moderate fits indicating partial but less reliable reconstruction. In contrast, Jishui Estuary subsites displayed distinct maxima with stronger fits (R2 > 0.85): JS-C, MAJS-C(t) = −0.0201(t − 14.3)2 + 7.0 peaking in 1996; JS-D, MAJS-D(t) = −0.0093(t − 15.8)2 + 2.2 peaking in 1998; and JS-G, and MAJS-G(t) = −0.0077(t − 11.6)2 + 4.3 peaking in 1994. SPOT-6 satellite imagery (22 February 2025) identified 281.9 ha of mangrove and windbreak forests in Chiayi County and 896.3 ha in Tainan City. By integrating climate records, subsidence data, sea level rise, polynomial modeling, and satellite observations, this study provides a robust framework for anticipating mangrove trajectories, assessing carbon sink potential, and refining carbon credit estimates in vulnerable coastal landscapes.

Ecologies doi: 10.3390/ecologies7010020

Authors: Fredah Cherotich Diba Galgallo Ram Dhulipala Anthony Whitbread Ambica Paliwal

The invasion of Prosopis juliflora poses a growing threat to dryland ecosystems and pastoral livelihoods across East Africa. This study presents an integrative approach that combines satellite remote sensing, machine learning, and citizen science to detect and map the spatial extent and socio-ecological impacts of Prosopis juliflora in Baringo County, Kenya. We evaluated the performance of three satellite platforms, Sentinel-1, Sentinel-2, and PlanetScope, using a Random Forest classifier trained on field collected presence–absence data and vegetation indices. Sentinel-2 outperformed the other sensors, achieving a classification accuracy of 90.65%, with key variables including the Visible Atmospherically Resistant Index (VARI), the Ratio Vegetation Index (RVI), and red-edge bands emerging as the most important predictors. Through Participatory GIS (PGIS), a citizen-science based approach, we engaged gender-disaggregated community groups to capture local perceptions of invasion hotspots and blocked access to grazing routes and water sources, enhancing contextual understanding and validating model outputs. The comparison of satellite-derived maps and PGIS outputs revealed strong spatial congruence, particularly along water bodies, roads, and croplands. Our findings demonstrate the potential of combining Earth observation and citizen science to generate actionable knowledge for managing invasive species in data scarce dryland environments. This hybrid framework supports inclusive and spatially targeted interventions for rangeland restoration and ecosystem resilience.

Ecologies doi: 10.3390/ecologies7010019

Authors: Camryn Lachica Althea Hotaling Hagan Kyle Barrett Debabrata Sahoo Calvin Sawyer Jeremy Pike

Ecological restoration has emerged as a critical tool for addressing ecosystem degradation worldwide. The Stream Quantification Tool (SQT) represents a significant advancement in stream restoration methodology, providing a standardized framework for quantifying stream functions and evaluating project outcomes. We investigated relationships between salamander abundance and physicochemical parameters from the SQT (nitrogen, turbidity, temperature, and composite SQT physicochemical score) across 16 streams in the upstate region of South Carolina. Stream salamanders can be reliable bioindicators due to their philopatric nature, longevity, and stable populations, making them valuable for assessing stream restoration effectiveness. Six salamander species (n = 394 individuals) were identified across 14 streams where salamanders were present. N-mixture models were used to analyze abundance patterns while accounting for imperfect detection. Turbidity was the strongest negative predictor of salamander abundance, followed by nitrogen. Removal of an agriculturally impacted stream with elevated turbidity substantially altered model rankings, making the null model top-ranked, followed by nitrogen, composite physicochemical score, and temperature. These findings demonstrate turbidity’s critical role in salamander abundance. Management practices should prioritize erosion and sediment control solutions for salamander conservation. For SQT biological monitoring, we recommend focusing on turbidity and nitrogen as key parameters affecting salamander abundance in stream assessments.

Ecologies doi: 10.3390/ecologies7010018

Authors: Petru Cuza Tatiana Sîrbu Pavel Pînzaru

Environmental changes and anthropogenic pressures significantly influence both the tree layer and natural regeneration within forest ecosystems. Protected areas represent essential territories for the maintenance and conservation of species within forest communities. In this context, the present study aims to develop a methodological framework for the integrated application of diversity, evenness, and dominance indices in the study of forest plant communities. Analyses were conducted at both α- and β-diversity levels, providing a methodological basis for characterizing local diversity and community differentiation. Species diversity was estimated using the Shannon–Wiener (H′) and Simpson (D) indices, while evenness and dominance were assessed using the Pielou (J′) and Berger–Parker (d) indices. Differences among communities were quantified using the Bray–Curtis dissimilarity index and its components, turnover and nestedness, and structural convergence of forest communities was analyzed through the ICF. The results indicate that α-diversity, estimated by H′, ranges from low to moderate, suggesting a relatively uniform distribution of species abundance. In certain microhabitats, processes of diversification and oligodominance are observed. At the β-diversity level, the analyzed communities are characterized by high dissimilarity, mainly driven by species turnover and, to a lesser extent, by nestedness associated with species loss. The ICF highlights that these forest communities exhibit relatively high structural uniformity, characteristic of mature stands in ecological equilibrium.

Ecologies doi: 10.3390/ecologies7010017

Authors: Vassilis Kypraios-Skrekas Alexis Lazaris Lydia K. Koutrouditsou Konstantinos Sotiropoulos Sinos Giokas

Understanding how environmental conditions shape the functional composition of ecological communities is a central goal in community ecology. In this study, we apply this framework to the reptile and amphibian assemblages within Greece’s Mount Chelmos protected area. Based on comprehensive field surveys (2018–2021) across 168 sampling stations, we compiled species trait databases and quantified functional diversity using a corrected Rao’s Q index. We modeled the response of functional diversity to climate, land cover, topography (altitude, slope, aspect), geographic location, and taxonomic diversity, using Generalized Additive Models (GAMs). Additionally, we examined traitspace structure via PCA and evaluated environmental drivers of trait composition with multivariate GAMs. For reptiles, functional diversity was significantly affected by altitude, climate, and aspect, with higher values predicted in water-associated marginal zones surrounding the mountain massif. Traitspace analysis revealed clear ecological structuring along axes related to locomotion, body size, reproductive mode, foraging strategy, and substrate use, shaped by distinct combinations of environmental filters. In amphibians, environmental effects on functional diversity were not statistically significant; however, traitspace showed discernible responses to land cover, climate, and aspect, suggesting weaker—though detectable—filtering processes. Collectively, our findings indicate that Mount Chelmos functions as a system that modulates diversity, with environmental filters operating at fine-to-medium spatial scales to shape the functional composition and diversity of its herpetofauna.

Ecologies doi: 10.3390/ecologies7010016

Authors: Thayná Kelly Formiga de Medeiros Gleyse Lopes Fernandes de Souza Francisco Thiago Coelho Bezerra Jackson Silva Nóbrega Igor Eneas Cavalcante Francisco Eudes da Silva Dayane Gomes da Silva Daniela Rosário de Mello Marcos Vinícius da Silva Maria Beatriz Ferreira Alberto Soares de Melo Alberício Pereira de Andrade Olaf Andreas Bakke Ivonete Alves Bakke Riselane de Lucena Alcântara Bruno

Water scarcity, intensified by climate change, limits the growth and survival of threatened tree species, such as Paubrasilia echinata (Lam.) Gagnon, H.C.Lima & G.P.Lewis. This study analyzed the effects of water availability in the soil on the morphophysiology and enzymatic activity of P. echinata seedlings under different irrigation intervals. The experiment consisted of five treatments (irrigation intervals: 0, 3, 6, 9, and 12 days) distributed in four randomized blocks, totaling 20 plots of five plants each. Variables analyzed included stem diameter, number of leaves and leaflets, leaf area, shoot and root length, root volume, dry matter mass, Dickson quality index, relative water content, leaf indices of chlorophyll, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase. Water deficit reduced root dry matter mass by up to 27.78% and chlorophyll b by up to 29.56%, and increased peroxidase by up to 244.44%. Principal component analysis revealed correlated changes among biomass, chlorophyll, and Dickson index, with root volume and enzymatic activities varying oppositely, indicating an integrated regulatory response. P. echinata exhibits phenotypic plasticity under water deficit, reflected in stem diameter, root volume, leaf area, physiological traits, and enzymatic regulation, tolerating an irrigation interval of 12 days. P. echinata has establishment potential in areas subjected to water deficit.

Ecologies doi: 10.3390/ecologies7010015

Authors: Ujjwal Layek Ram Kumar Bhakat Prakash Karmakar

Several honeybee species can coexist within a given geographical region; however, their interactions and the extent to which their foraging niches overlap remain poorly understood. To address this gap, we assessed niche overlap among four honeybee species (Apis cerana, Apis dorsata, Apis florea and Apis mellifera) in West Bengal, India, using palynological analysis of corbicular pollen loads. The monofloral composition of individual corbicular loads indicated flower constancy in all species. A wide range of pollen types was recorded for each species, confirming their polylectic foraging behaviour. Although the level of pollen diversity in their diet did not differ among species, it did vary seasonally. Vital pollen sources (including Acacia auriculiformis, Borassus flabellifer, Brassica juncea, Bridelia retusa, Cocos nucifera, Coriandrum sativum, Eucalyptus tereticornis, Lannea coromandelica and Sesamum indicum) were shared by all four species, and their pollen-foraging niches showed significant overlap in every season. Considering the floral traits of the plants visited, all honeybee species behaved as generalist flower visitors. This study quantifies foraging niche overlap among honeybee species and identifies common floral resources shared by the exotic Apis mellifera and native honeybees. Such insights will assist in honeybee management, biodiversity conservation, and the enhancement of ecosystem services and stability.

Ecologies doi: 10.3390/ecologies7010014

Authors: Brian Omwoyo Joana M. Hancock Leah Mainye Jane R. Lloyd Stephanie Köhnk Mumini Dzoga Cosmas Munga

Globally, sea turtles face significant threats from human activities, yet detailed information on their habitat use and specific anthropogenic impacts remains limited, particularly in key marine protected areas like Kenya’s Diani-Chale National Marine Reserve (DCNMR). This study utilized drone-based (UAV—unmanned aerial vehicle) monitoring and geospatial analysis to assess sea turtle distribution and habitat use, integrating data from the Allen Coral Atlas. Most sea turtle sightings occurred in reef zones (61.86%), while the reef slope was the most utilized geomorphic feature (26.7% of sightings). The study identified a significant sea turtle hotspot in the northern DCNMR, a region characterized by lower anthropogenic pressure and unique geomorphic features. Between February and July 2024, we conducted monthly UAV surveys (6–10 survey days per month) in the DDCNMR using a DJI Mavic 3 drone, completing multiple standardized 25-min flights per day that each covered ~1 km2 via non-overlapping transects at 30–40 m altitude under optimal sea state and visibility conditions, resulting in 233 sea turtle sightings. UAV survey data were summarized descriptively, with sea turtle sightings mapped against geomorphological features as well as benthic habitats from an open source, high-resolution, satellite-based map and monitoring system for shallow-water coral reefs (ACA—Allen Coral Atlas). Allen Coral Atlas data and drone observations indicate that a widened reef slope and estuarine nutrient inputs provide a critical habitat gradient, offering turtles tidal-independent access to shallow foraging flats. Based on these findings, we recommend designating the northern reef slope as a priority no-take zone and conducting seagrass health assessments to guide potential restoration. This research demonstrates the utility of integrating drone surveys with open access geospatial tools to provide the actionable spatial data necessary for targeted sea turtle conservation and informed marine spatial planning.

Ecologies doi: 10.3390/ecologies7010013

Authors: Xiaolong Zhang Xianmeng Liu Dingrou Yao Yongji Wang Junjie Niu Yinbo Zhang

The authors would like to make the following corrections to the published paper [...]

Ecologies doi: 10.3390/ecologies7010012

Authors: Júlia Resende Oliveira Silva Décio Karam Kassio Ferreira Mendes

Knowledge of weed community structure in agricultural systems is important for sustainable management, especially for evaluating the effects of different herbicides on soybean–corn succession crops. This study evaluated, over two crop seasons, weed community structure in response to chemical weed management strategies for soybean–corn succession in Brazil’s Triângulo Mineiro region. Phytosociological surveys of the weed community were conducted during harvest periods throughout the experimental phase, with referenced data for generating spatial distribution maps of biomass and density of the main present species. The survey identified 33 weed species, predominantly from the Poaceae and Asteraceae families. Regardless of the management system, the total weed biomass was lower in corn crops compared to soybean crops. In management systems using six different herbicides, the IVI of Commelina benghalensis was the lowest due to greater diversification of herbicide mechanisms of action. The results demonstrate that chemical weed management strategies strongly influence weed community structure, with significant effects on weed community structure and evenness in intensive agricultural regions. These changes also have implications for resistance management.

Ecologies doi: 10.3390/ecologies7010011

Authors: Theodore Zoulias Alexis Conides Sofia Reizopoulou Dimitris Vafidis Dimitris Klaoudatos

Coastal lagoons are highly vulnerable to human and climatic pressures, yet long-term ecological changes remain poorly quantified. We analyzed four decades (1980–2020) of data from fisheries from six lagoons in the Amvrakikos Gulf, Greece, using ecological indicators to assess trophic structure, exploitation status, and ecosystem responses. Cluster analysis of species level fishery production revealed a distinct temporal regime shift in the late 1990s–early 2000s, reflecting a major reorganization of species contributions to total yield. Mean total yield (Y), showed a consistent declining trend across lagoons, ranging from 2.7 ± 2.0 to 7.2 ± 5.0 t km−2. Primary Production Required (PPR) declined (0.8–1.5 × 1010g C km−2 yr−1), while Mean Temperature of the Catch (MTC) increased in five lagoons (19.0–21.4 °C) and remained stable in one (20.0 ± 0.9 °C). Pelagic to demersal (P/D) ratios generally decreased (0.09–1.26), and Q-90 values were variable (0.8–2.2), highlighting site specific ecological dynamics. Short term yield predictions for 2021–2025 ranged from 0.78 to 6.75 t km−2, remaining comparable to recent historical levels, while the estimated carrying capacities varied from 1.79 to 9.11 t km−2, reflecting contrasting exploitation states among lagoons. These results demonstrate that multi-indicator, fishery-based analyses provide a robust framework for quantifying ecological change and guiding adaptive management in lagoon ecosystems.

Ecologies doi: 10.3390/ecologies7010010

Authors: Sihle Edmund Mthethwa Sellina Ennie Nkosi

Invasive alien plant species pose significant threats to biodiversity and the ecological functioning of ecosystems, necessitating targeted clearing strategies. This study investigated the short-term recovery of native vegetation following the control of Lantana camara and Chromolaena odorata in communal lands of Manzini, Eswatini. Nineteen sites were sampled across cleared and uncleared areas to assess changes in species diversity and veld condition. Cleared sites showed slightly reduced heterogeneity (D′ = 0.722) and higher diversity (H′ = 2.081) compared to uncleared sites (D′ = 0.732) and diversity (H′ = 2.032). Sites free from invasive alien plants had higher species richness (EXP (H′) = 35.693) than invaded sites (EXP (H′) = 28.237). Although statistical analyses showed no significant differences in stem counts between cleared and uncleared sites, effect sizes indicated potential practical significance for C. odorata. The Veld Condition Index (VCI) revealed high spatial variability with no consistent trend associated with clearing. Findings emphasise the complexity of early post-clearing dynamics and the importance of site-specific follow-up and monitoring.

Ecologies doi: 10.3390/ecologies7010009

Authors: Xiaolong Zhang Xianmeng Liu Dingrou Yao Yongji Wang Junjie Niu Yinbo Zhang

Understanding how plant community characteristics and soil properties vary along altitudinal gradients is essential for ecosystem conservation, restoration, and for predicting ecosystem responses to global environmental change. This study investigated altitudinal patterns and their potential drivers in mountain meadow communities on the southern slope of Wutai Mountain, Northern China. Community characteristics and soil physicochemical properties were measured along an altitudinal gradient ranging from 1800 to 3000 m a.s.l. Most community characteristics exhibited clear altitudinal trends. Species richness, Shannon–Wiener index, Simpson index, aboveground biomass and average plant height all declined significantly with increasing altitude. In contrast, vegetation cover showed a unimodal pattern, initially decreasing and then increasing at higher elevations. Soil physicochemical properties also varied significantly along the altitudinal gradient and were closely associated with changes in community characteristics. Variation partitioning analysis revealed that environmental factors, including altitude and soil properties, explained 71.9% of the total variation in mountain meadow communities. Altitude alone contributed more to community variation than soil factors, indicating its dominant role in shaping community structure. Nevertheless, specific soil properties, particularly soil depth, soil bulk density and soil pH, also exerted significant influences on community characteristics. Overall, our results demonstrate that altitude is a key driver of both vegetation and soil variation in mountain meadows on the southern slope of Wutai Mountain. In addition to altitudinal effects, soil physicochemical properties should be considered when developing conservation and management strategies for mountain meadow ecosystems.

Ecologies doi: 10.3390/ecologies7010008

Authors: Magaly I. Aulestia-Herrera Pedro Romero-Saker Wilmer E. Pozo-Rivera

In northwestern Ecuador, where more than 90% of the original forest cover has been lost, it is unknown how soil chemistry influences bat diversity. This study evaluated bat diversity, non-herbaceous plant community structure, and soil nutrients in 30 plots distributed across crops on two farms separated by 32 km. Soil analyses revealed variations in organic matter and nutrients, identifying calcium, magnesium, zinc and iron as the most influential. A total of 1662 individuals of 24 non-herbaceous plant species and 193 individuals of 16 bat species were recorded, dominated by frugivorous and nectarivorous guilds. Generalized linear mixed models showed significant relationships between bat diversity indices and soil nutrients. These elements improve tree growth, fruiting, and flowering, which increases the quality and availability of food resources for bats. In return, these mammals provide key ecosystem services such as pollination, seed dispersal, and insect control. Our findings highlight that soil chemistry indirectly regulates bat communities by influencing vegetation structure and resource availability. This integrated approach underscores the importance of soil–plant–animal interactions in tropical agricultural landscapes, offering practical guidance.

Ecologies doi: 10.3390/ecologies7010007

Authors: Shulin Yang Yongpiao Yu Hegen Zeng Jie Liu

Chestnut is an important economic plant for ecology and farmers in mountainous areas in China. We conducted surveys and experiments to assess the economic value of the pollination service provided by insect pollinators for chestnuts via the Toolkit for Ecosystem Service Site-based Assessment (TESSA) in Bapeng Village, Wangmo County, Guizhou Province of China. We applied three methods presented by TESSA, the desk-based method, the field survey method, and the exclusion experiment, for the assessment. The resulting pollination values for the three methods are (1) CNY 3712.5∙ha−1∙yr−1 in the assessment site and an average of CNY 1386∙ha−1∙yr−1 with buffer zones included for the desk-based method; (2) CNY 3712.5∙ha−1∙yr−1 in the assessment site and an average of CNY 1124∙ha−1∙yr−1 with buffer zones included for the field survey method; and (3) CNY 4158∙ha−1∙yr−1 in the assessment site and an average of CNY 1485∙ha−1∙yr−1 with buffer zones included for the exclusion experiment method. The total value of chestnut pollination of the Bapeng Village ranges from CNY 311,943 yr−1 to CNY 404,663 yr−1. The chestnut pollination of the village is substantially insufficient. This could be the result of the decrease in diversity and uneven distribution of insect pollinators which, per se, are caused by the lack of larval hosts for those pollinators.

Ecologies doi: 10.3390/ecologies7010006

Authors: Joseph S. Wilson Lindsey Topham Wilson Tyler M. Wilson Michael Carter Zabrina Ruggles

We surveyed bee communities across an organic conifer tree farm landscape in northern Idaho to assess how managed forest–agriculture mosaics support pollinator diversity. Bees were collected from farm fields, adjacent conservation forests, and a pollinator garden between May and August 2024 using aerial nets and identified to species or morphospecies. In total, 94 bee species were recorded, representing a mix of ground-nesting (46%), cavity-nesting (37%), and social (17%) taxa. Bee richness was highest in farm fields (66 species), intermediate in forests (48 species), and lowest in the pollinator garden (35 species). Community turnover among habitats was substantial (Jaccard dissimilarity = 0.67–0.76; Bray-Curtis dissimilarity = 0.53–0.55), indicating distinct assemblages associated with each habitat type. Comparisons with regional datasets from Montana and Washington revealed moderate overlap (Jaccard = 0.22–0.24), suggesting that the Highland Flats farm supports a partly unique bee fauna within the Northern Rockies. Seven non-native bee species and nine species of conservation concern (five Osmia, four Bombus) were detected, with those of conservation concern taxa often visiting native Lupinus flowers. Most bee visits occurred on non-native plants, though native blooms contributed key seasonal resources. These findings demonstrate that organic tree farms with structurally diverse forests and managed floral resources can function as refugia for both common and at-risk bees in temperate forested landscapes.

Ecologies doi: 10.3390/ecologies7010005

Authors: Jan Winkler Václav Zámečník Amir Mugutdinov Petra Martínez Barroso Magdalena Daria Vaverková

Generally, all European countries have reported a decreasing number of field birds. The cause of this trend is the intensification of agriculture, including inconsiderate landscape and drainage measures. Northern Lapwings (Vanellus vanellus L.) can be protected using targeted agri-environmental climatic measures (AECMs). The goal of our research was to verify whether the vegetation composition on arable land areas intended to protect field birds creates space for the promotion of other organisms. Understanding the significance of AECMs in supporting biodiversity on arable land will help guide the development of ecosystems in agricultural landscapes. The research was conducted in two regions of the Czech Republic (CR): Southern Bohemia (six sites) and Eastern Bohemia (six sites), and 76 plant taxa were identified in areas of arable land intended for the protection of Northern Lapwing. The vegetation of arable land managed to protect the Northern Lapwing was statistically shown to exhibit a plant species richness approximately four times greater than crop land. Measures focused on the protection of one species create a living space that can be used by other species. AECMs can be used to maintain or increase biodiversity and heterogeneity in agricultural landscapes. Our findings emphasize the need for a holistic approach to conservation in agricultural settings, where the protection of one focal species can lead to cascading benefits for the entire ecosystem. This research provides valuable insights into how AECMs can be a pivotal tool in mitigating the detrimental impacts of agricultural intensification on field birds and broader biodiversity in Europe.

Ecologies doi: 10.3390/ecologies7010004

Authors: Pyrus Flavien Ebouel Essouman Timothée Besisa Nguba Franck Robéan Wamba Charles Mumbere Musavandalo Louis Pasteur Bopoko Bamenga Isaac Diansambu Makanua Jean-Pierre Mate Mweru Baudouin Michel

The Congo Basin peatlands, the world’s largest tropical peatland complex, are critical for global carbon storage yet remain poorly understood from a human dimension’s perspective. This study explores the perceptions, lifestyles, and knowledge systems of Indigenous Peoples and local communities around Lake Tumba, Democratic Republic of Congo, to identify practices supporting peatland conservation. Using a mixed-methods approach—household surveys (n = 320), focus groups, and statistical analyses including chi-square tests and Multiple Correspondence Analysis (MCA)—the study reveals a predominantly Indigenous agrarian society with limited formal education and strong reliance on peatlands for food (93.7%), construction materials (79.0%), and medicines (75.9%). While regulating services such as carbon storage were seldom recognized, traditional ecological knowledge was evident in sacred species protection, ritual plant and animal uses, and intergenerational knowledge transfer, mainly father-to-son. However, 95.3% of respondents cited religion as the main barrier to this transmission. MCA confirmed that livelihoods, village status, and ritual practices form an integrated socio-cultural system aligned with conservation. These findings stress the role of endogenous governance in sustaining peatland-compatible lifestyles. Conservation efforts should move beyond carbon-centered or top-down approaches to reinforce land tenure, traditional governance, and knowledge transmission, thereby protecting both peatlands and the cultural identities sustaining them.

Ecologies doi: 10.3390/ecologies7010003

Authors: Volker Lüderitz

Rivers and their associated landscapes—floodplains, wetlands, and oxbow lakes—represent some of the most dynamic and biologically rich ecosystems on Earth [...]

Ecologies doi: 10.3390/ecologies7010002

Authors: Tiago Monteiro Condé Niro Higuchi Adriano José Nogueira Lima Moacir Alberto Assis Campos Joaquim Dos Santos Bruno Oliva Gimenez Fabiano Emmert Vilany Matilla Colares Carneiro

The northern Brazilian Amazon has ecological transition ecosystems with high diversity and endemism of tree species and few botanical collections. We evaluated the phytosociology between Dense Ombrophilous Forest (Ds) and Forested Campinarana (Ld) within Anauá National Forest in Roraima, Brazil. A total of 14,730 trees with a DBH ≥ 10 cm were inventoried across 30 hectares (ha), distributed among 55 botanical families, 183 genera, 386 species, and 123 undetermined trees. Ten hyperdominant tree families accounted for 69% of the sampled trees and 65% of the stored forest carbon (102.9 ± 5.0 Mg ha−1), like Arecaceae (2555 trees), Fabaceae (1738 trees), and Sapotaceae (1311 trees). Ten hyperdominant species accounted for 32% of the sampled individuals and 32% of the stored forest carbon (46.3 ± 3.8 Mg ha−1), like Euterpe precatoria (1151 trees), Pouteria macrophylla (561 trees) and Inga alba (574 trees). Anauá National Forest has great potential for sustainable multiple-use forest management through forest concessions; however, tree mortality due to natural causes and anthropogenic actions (deforestation, illegal selective logging, and forest fires) was considered high (7%) for tropical forests in the Amazon.

Ecologies doi: 10.3390/ecologies7010001

Authors: Guadalupe Gómez-Carrasco Julia María Lesher-Gordillo León David Olivera-Gómez Erick de la Cruz-Hernández Benjamín Morales-Vela Gilberto Pozo-Montuy Salima Machkour-M’Rabet Hilda María Díaz-López

Aquatic ecosystems are currently facing anthropogenic pollution, mainly derived from agricultural, industrial, and urban runoff, including heavy metals, polycyclic aromatic hydrocarbons (PHAs), pesticides, fertilizers, and untreated wastewater discharges. To understand the impact of environmental contamination on fish, this research compared cytochrome P450 1A (CYP1A) gene expression in armored catfish across three locations in the lower Grijalva–Usumacinta River basin known for varying levels of pollution. Samples from the Ribera Alta, the Bitzales River, and the Chaschoc lagoon were collected during the dry and rainy seasons. We isolated RNA from liver samples, which were subsequently converted to cDNA. We used quantitative PCR to analyze CYP1A gene expression. Results showed that, of the three locations, Ribera Alta demonstrated the highest expression during the rainy season. Only in Chaschoc Lagoon did we observe significant differences between seasons (p = 0.03). This indicates that seasonal factors and the presence of pollutants in the water bodies and sediments likely play a role in regulating CYP1A gene expression in this fish species.

Ecologies doi: 10.3390/ecologies6040086

Authors: Ana Cano Ortiz José Carlos Piñar Fuentes Ricardo Quinto Canas Eusebio Cano

The teaching of the phytosociological method comprises several stages and aligns closely with the research-oriented teaching–learning process promoted by active methodologies. In both cases, preliminary inquiry is essential to review existing knowledge on vegetation in all its dimensions: bioclimatic, biogeographical, ecological, floristic composition, distribution, and conservation status. The main objective is to connect active teaching methodologies with phytosociological research. To this end, the natural environment is used to bring students into direct contact with plant communities, and the phytosociological research method is applied, through which students learn sampling techniques. This approach provides a rapid and effective assessment of habitat conservation status (EU Habitats Directive 92/43/EEC, European Council, 21 May 1992). As notable results, we highlight the poor conservation status of the three communities described, which is evident from the decline in characteristic association species. The present study focuses on the wetlands of the Costa Tropical, where communities of Juncus acutus, Typha dominguensis, Phragmites australis, and Arundo donax predominate. In this case, these communities act as open-air laboratories for teaching the phytosociological method. The Juncus acutus communities differ from those of Scirpus holoschoenus and other Juncus acutus stands by the presence of the endemic Linum maritimum. Meanwhile, the reedbeds differ from Thypho-Phragmitetum australis through the presence of Halimione portulacoides. In both cases, the influence of sea spray conditions the presence of subhalophilous species such as Juncus acutus, Linum maritimum, and Halimione portulacoides. This has enabled us to establish two new plant associations: LmJa = Lino maritimi–Juncetum acuti (rush stands) and Hp–Phra = Halimione portulacoidis–Phragmitetum australis (reedbeds). Ecological gradients also make it possible to separate Typha communities belonging to the Ca–Td = Cynancho acuti–Typhetum dominguensis association, and Phragmites into two distinct associations. This distinction arises because Typha communities require soil water during the summer period, whereas in Phragmites stands the upper soil horizon dries out.

Ecologies doi: 10.3390/ecologies6040085

Authors: Jingqing Wu Cong Liu Changhui Peng Binggeng Xie Zelin Liu

As critical reservoirs of biodiversity and providers of ecosystem services, wetland ecosystems play a pivotal role in maintaining global ecological balance. They not only serve as habitats for diverse aquatic and terrestrial organisms but also play substantial roles in water purification, carbon sequestration, and climate regulation. However, intensified anthropogenic activities—including drainage, fertilization, invasion by alien species, grazing, and urbanization—pose unprecedented threats, leading to profound alterations in the functional traits of wetland plants. This review synthesizes findings from peer-reviewed studies published between 2005 and 2024 to elucidate the mechanisms by which human disturbances affect plant functional traits in wetlands. Drainage was found to markedly reduce plant biomass in swamp ecosystems, while mesophyte and tree biomass increased, likely reflecting altered water availability and species-specific adaptive capacities. Mowing and grazing enhanced aboveground biomass and specific leaf area in the short term but ultimately reduced plant height and leaf dry matter content, indicating potential long-term declines in ecological adaptability. Invasive alien species strongly suppressed the growth of native species, reducing biomass and height and thereby threatening ecosystem stability. Eutrophication initially promoted aboveground biomass, but excessive nutrient inputs led to subsequent declines, highlighting ecosystems’ vulnerability to shifts in trophic state. Similarly, fertilization played a dual role: moderate inputs stimulated plant growth, whereas excessive inputs impaired growth performance and exacerbated eutrophication of soils and water bodies. Urbanization further diminished key plant traits, reduced habitat extent, and compromised ecological functions. Overall, this review underscores the profound impacts of anthropogenic disturbances on wetland plant functional traits and their cascading effects on ecosystem structure and function. It provides a scientific foundation for conservation and management strategies aimed at enhancing ecosystem resilience. Future research should focus on disentangling disturbance-specific mechanisms across different wetland types and developing ecological engineering and management practices. Recommended measures include rational land-use planning, effective control of invasive species, and optimized fertilization regimes to safeguard wetland biodiversity, restore ecosystem functions, and promote sustainable development.

Ecologies doi: 10.3390/ecologies6040084

Authors: Sandra Guadalupe García-Aburto Graciela Nani Vicente Vergara-Flórez David Reyes-González Erick Arturo Betanzo-Torres Alexi Peralta-Vega Luis Carlos Sandoval Herazo

Water pollution caused by petroleum-derived volatile organic compounds such as benzene, toluene, ethylbenzene, and xylenes (BTEX), as well as methyl tert-butyl ether (MTBE), poses a growing threat to aquatic ecosystems and human health. These contaminants, together with the organic matter and nutrients present in municipal wastewater, highlight the need for sustainable treatment technologies adapted to tropical conditions. This study evaluated the removal efficiency of BTEX, MTBE, and conventional pollutants using hybrid constructed wetlands (HCWs) that combine vertical subsurface flow (VSSF-CW) and horizontal subsurface flow (HSSF-CW) systems. Two plant species—Heliconia latispatha and Phragmites australis—were tested, along with a polyculture and an unvegetated control. The hybrid systems treated synthetic influents formulated to simulate contaminated municipal wastewater. Parameters including COD, TSS, N–NH4+, N–NO3−, P–PO43−, BTEX, and MTBE were monitored and analyzed using ANOVA and Tukey’s test (p < 0.05). Vegetated systems achieved COD removal efficiencies exceeding 85%, compared with 72% in the control. Phragmites australis obtained the highest removal of suspended solids (92 ± 3%) and ammonium nitrogen (88 ± 2%), whereas Heliconia latispatha exhibited superior phosphorus removal (84 ± 4%). The polyculture displayed a synergistic effect, achieving removal rates of 93% for benzene, 91% for toluene, and 88% for MTBE, with statistically significant differences relative to the control (p < 0.05). In conclusion, hybrid constructed wetlands planted with Heliconia latispatha and Phragmites australis demonstrated high efficiency and stability in removing BTEX, MTBE, and conventional pollutants under tropical conditions, positioning themselves as a sustainable, low-cost, and esthetically valuable treatment alternative.

Ecologies doi: 10.3390/ecologies6040083

Authors: Raf Ana Rabbi Shawon Md. Matiur Rahman Md Mehedi Iqbal Haris Debbarma Junji Moribe

The monitoring of wildlife habitats is crucial for effective conservation efforts, particularly where biodiversity faces significant threats. This study aimed to monitor the biodiversity of wild mammals in a protected area (PA) of Northeastern Bangladesh, with a particular focus on detecting previously unrecorded species using camera traps. We deployed nine camera traps across 19 locations inside the PAs of Satchari National Park (SNP) from May 2024 to April 2025. Further, the camera-trap data were analyzed to evaluate the existing wild mammals, along with their activity patterns and seasonal variations, in SNP. Our study identified the Asiatic black bear in SNP for the first time, representing a significant contribution to biodiversity records of Bangladesh. Among the other frequently documented wild mammals were the wild boar, northern pig-tailed macaque, and barking deer, whereas less commonly detected species included the crab-eating mongoose and jungle cat. Activity pattern analysis of Asiatic black bear revealed a predominantly nocturnal-to-crepuscular behavior, with distinct bimodal peaks during early morning and evening. The present study showed that the Asiatic black bear was active in pre-monsoon and winter; however, it was absent during the rainy season, suggesting seasonal habitat use or detectability challenges. This is the first study to confirm the presence of Asiatic black bears in PAs of SNP using camera traps. These findings also highlight the importance of long-term biodiversity monitoring for continued conservation efforts to protect the diverse wildlife of SNP. The detection of previously undocumented wild mammals highlights the ecological importance of SNP, urging authorities to tighten the ongoing conservation initiatives. Understanding the diel and seasonal activity patterns would instruct the timing of conservation and habitat management strategies. This study also makes the integration of camera-trap monitoring into long-term biodiversity management to guide evidence-based conservation policies in Bangladesh’s PAs.

Ecologies doi: 10.3390/ecologies6040081

Authors: Shynbolat Rsaliyev Amangeldy Sarbaev Aidarkhan Eserkenov Sholpan Bastaubayeva Nurbakyt Orazaliev Arman Baimagambetov Kanat Yermekbayev

The Sunn pest (Eurygaster integriceps Puton) ranks among the most harmful pests affecting wheat yield and grain quality in Kazakhstan. In particular, it poses a serious threat to regions in which winter wheat cultivation is dominant. Climate change, parasites, predators, and recent transformations in agriculture and human activities in Kazakhstan and throughout Central Asia have significantly influenced the population dynamics of the Sunn pest. This study reports the findings on Sunn pest population dynamics in Kazakhstan’s winter wheat growing regions from 2022 to 2024, based on surveys of 233 hectares across four regions. In total, 1753 specimens of the Sunn pest were studied. The obtained results were analyzed in comparison with historical data (1991–2020) and recent findings in this field. We found that a combination of ecological factors are the main determinants of the Sunn pest population dynamics in different regions of the country. The pest population increased in seasons with optimal temperature (sum of effective temperatures—SET) and humidity conditions (hydrothermal coefficient—HTC), as well as when wheat cultivation areas and forest belts expanded. Moreover, the results highlighted that the pest population is controlled by the activity of egg parasites (Telenomus) in the south, unfavorable weather conditions during overwintering in the east and west, and the growing of resistant varieties in the southeast of the country. Compared to wild grasses, wheat crops increased the reproductive potential of the pest.

Ecologies doi: 10.3390/ecologies6040082

Authors: Reshma Sunkur

Invasive alien species (IAS) are a significant threat to ecosystems worldwide, in particular island ecosystems where ecological resilience is limited. Spatially explicit and locally informed strategies are crucial on small islands to effectively manage IAS. The present study uses an integrated approach to map and manage IAS on Rodrigues Island, Mauritius, using a combination of field surveys, participatory mapping, and spatial analysis tools. Field data was collected in four sites on Rodrigues, namely Cascade Pigeon, Cascade St Louis, Mourouk Valley, and Golden Bat Reserve, supported by participatory mapping and Inverse Distance Weighting (IDW) interpolation in ArcGIS. The results revealed firstly that invasion hotspots were concentrated in previously disturbed areas, especially in Mourouk Valley and Cascade Pigeon, where Furcraea foetida, Leucaena leucocephala, and Millettia pinnata co-occur. Secondly, grassland zones exhibited minimal invasion, indicating their potential as natural buffer zones for conservation. Thirdly, the integration of stakeholder knowledge through Participatory GIS (PGIS) enhanced the accuracy and contextual understanding of the spatial analysis. Fourthly, the IDW interpolation method demonstrated high precision with low root mean square error (RMSE) values and minimal spatial error (≤0.5 m). Finally, the study underscores the importance of adaptive, site-specific monitoring and management strategies that combine spatial tools and local knowledge. These findings provide a replicable framework for IAS management in other island ecosystems facing similar ecological challenges, contributing to national and international biodiversity conservation efforts, including Sustainable Development Goal 15—Life on Land.

Ecologies doi: 10.3390/ecologies6040079

Authors: Pietro Orlando Manuel Scerra Cino Pertoldi Sussie Pagh Francesco Foti

The recolonization of the wolf (Canis lupus italicus) in Italy represents conservation success, but it has led to increased conflicts with livestock farming. These conflicts may undermine traditional pastoral practices, which are important for maintaining rural landscapes and associated biodiversity. In 2023, the European wolf population exceeded 20,300 individuals, with an estimated 65,000 livestock losses reported annually across the EU. This study assesses the effectiveness of an acoustic anti-wolf collar to complement existing protective measures, including fencing, human surveillance, and guarding dogs. A field trial was conducted from June to August 2024 in the municipality of Bova Marina in the metropolitan city of Reggio Calabria, Italy, using three groups of 50 Aspromonte goats. The groups were managed by: (1) a shepherd only (SO), (2) a shepherd with guarding dogs (SGD), and (3) a shepherd with guarding dogs and the anti-wolf collar (SGDC). The collar emitting modulated frequency intervals based on natural harmonic sounds, intended to deter wolves, was mounted on goats. Monitoring, by camera traps, enabled a comparative analysis of predation events. Camera data indicated persistent wolf activity at the site (54 images at CT1, 42 at CT2), but outcomes diverged by treatment. Two camera traps positioned at corridor bottlenecks identified from terrain morphology confirmed wolf presence and provided continuous coverage of the three groups on the single property. SO had 72 attacks and 5 kills; SGD had 26.39% fewer attacks and 1 kill; SGDC had no predation events despite confirmed presence. The preliminary findings suggest that the use of the anti-wolf collar may contribute to a reduction in predation and be a useful addition to strategies aimed at promoting coexistence between wolves and pastoral activities.

Ecologies doi: 10.3390/ecologies6040080

Authors: Saud S. Aloud Fahad Alotaibi Salah N. Sorrori Basil Alshebe

This study investigates the influence of rhizosphere dynamics and soil chemical properties on the distribution, abundance, and diversity of arbuscular mycorrhizal fungi (AMF) across two seasons (summer and winter). A total of 11 rhizospheric soil and root samples were collected from various wild plant species, including Senna italica, Cyperus laevigatus, Phragmites australis, Pelargonium peltatum, Zygophyllum simplex, Citrullus colocynthis, Malva parviflora, Zygophyllum coccineum, Calotropis procera, Solanum nigrum, and Salsola baryosm. Phragmites australis exhibited the highest AMF spore count (175 and 124/100 g dry soil in summer and winter), while Calotropis procera showed the lowest (101 and 63/100 g). AMF species identified included Glomus ambisporum, Rhizophagus intraradices, Claroideoglomus etunicatum, Diversispora globifera, Funneliformis geosporum, Funneliformis mosseae, Rhizophagus fasciculatus, and Gigaspora spp. The Shannon diversity index ranged from 0.692 (Zygophyllum simplex) to 0.653 (Salsola imbricata), and Simpson’s index from 0.498 to 0.461. Phragmites australis recorded the highest root colonization (90.5% and 84.7%), arbuscule (76% and 69.3%), and vesicle formation (36%) in summer, while Calotropis procera had the lowest. In summer, AMF spore counts showed significant correlations with soil nutrients (N, P, K), and with total organic carbon (TOC) and organic matter (OM). During winter, TOC and OM remained influential, while correlations with nutrients weakened. Soil pH, electrical conductivity (EC), and texture exhibited minimal correlation with AMF spore counts in both seasons.

Ecologies doi: 10.3390/ecologies6040078

Authors: Liliana Mora-Hernández Carlos Lara Mariana Cuautle Ubaldo Márquez-Luna Karla López-Vázquez

Loeselia mexicana (Polemoniaceae) is a Mexican shrub with significant medicinal value since pre-Hispanic times. Despite its ethnobotanical importance and apparent role in supporting pollinator communities, detailed information about its reproductive biology remains limited, hindering conservation efforts for this increasingly harvested species. We investigated the reproductive ecology of L. mexicana across two flowering seasons (2023–2024 and 2024–2025) in central Mexico through an integrated approach examining flowering phenology, floral morphology, sexual maturation sequence, nectar characteristics, floral visitors, and breeding system experiments. Flowering occurs from September to March, peaking in October. Flowers exhibit protandry, with anther dehiscence on days 1–2 and stigma receptivity from day 2 onward (flower lifespan: 2.85 ± 0.11 days). Maximum nectar production (1.46 ± 0.05 µL per flower; 193.13 ± 8.8 mg/mL) coincided with peak visitor activity. Despite possessing classic ornithophilous traits, we recorded 21 floral visitor species (5 hummingbirds, 3 hymenopterans, 13 butterflies) with similar visitation patterns, challenging previous assumptions about pollination specialization. Controlled pollination experiments confirmed self-incompatibility, with cross-pollination producing significantly more seeds than autonomous selfing. Our findings reveal that L. mexicana maintains a generalized pollination system, while protandry and self-incompatibility enforce outcrossing, providing critical baseline information for conservation strategies.

Ecologies doi: 10.3390/ecologies6040077

Authors: Katarina Mladenović Bojan Stojnić Biljana Vidović Danilo Furtula Djordje Jović

The species Petrobia (Tetranychina) harti (Ewing, 1909) is reported for the first time in Serbia, also representing the first record of the tribe Petrobiini Reck, 1952 in the country. Including this finding, the number of spider mite species recorded in Serbia now totals 45. This spider mite species was found exclusively on wood-sorrel plants, Oxalis corniculata L. and O. articulata Savigny., at several localities in the cities of Belgrade and Zrenjanin. The samples were collected during the growing season of 2018 and 2023. Identification of the oxalis mite was based on morphological characteristics. To date, the occurrence of P. (T.) harti on other host plants has not been confirmed anywhere in Serbia. On the observed wood-sorrel plants, P. (T.) harti was recorded in cohabitation only with Tetranychus urticae Koch, 1836.

Ecologies doi: 10.3390/ecologies6040076

Authors: Xueying Sun Zhongsi Gao Xiaogang Lin Qingming Wu Muhammad Suliman Jingli Zhu Hongfei Zou

The red-crowned crane (Grus japonensis) is a flagship species for wetland biodiversity in East Asia. The Songnen Plain is a vital wetland and habitat for rare and endangered birds in Northeast China. However, rapid land use changes have raised urgent concerns about habitat loss and the survival of these populations. We combined 30 years (1990–2020) of field surveys with ensemble species distribution models (SDMs) to analyze the spatio-temporal changes in suitable habitats for all three key life stages—spring migration, breeding, and autumn migration—across the Songnen Plain. We also assessed how well the current protected-area (PA) network covers suitable habitats and identified conservation gaps. Land use type was the most significant predictor of habitat suitability. Over this period, suitable habitats decreased sharply by 60% (spring migration), 72% (breeding), and 76% (autumn migration), with severe fragmentation and a clear northward shift. Core suitable areas are now mainly found within a few nature reserves, including Zhalong, Wuyu’er River, and Xianghai. We identified three significant conservation gaps: Lindian–Anda, Tailai–Dumeng, and Meilisi Daur–Fuyu. Our results show widespread habitat reduction and demonstrate the inadequacy of the current PA network in supporting the long-term survival of red-crowned crane populations. We recommend expanding protections and restoring wetland connectivity within these gaps to maintain critical habitats and improve landscape resilience for this endangered species.

Ecologies doi: 10.3390/ecologies6040075

Authors: Keying Qu Leichao Nie Lijuan Cui Huazhe Li Mingshuo Xiong Xiajie Zhai Xinsheng Zhao Jinzhi Wang Yinru Lei Wei Li

Soil nutrient detection in wetlands is critical for rapidly and effectively managing these ecosystems. Our objective was to provide a methodological framework for identifying optimal data processing methods and machine learning model for predicting soil organic carbon (SOC) and total nitrogen (TN) content using Vis–NIR spectroscopy, under the confounding influence of varying soil moisture. Soil samples (474) were collected from the Shaanxi Yellow River Wetland Provincial Nature Reserve with five moisture levels (0, 5, 10, 20, and 30%). Using a Vis–NIR spectroscopy system (ASD FS4 spectrometer), soil organic carbon (SOC) and total nitrogen (TN) were detected within the 350–2500 nm spectral range. Machine learning models were established using the Random Forest model (RF), eXtreme Gradient Boosting (XGBoost), and Partial Least Squares Regression (PLSR). The results indicated: (1) spectral reflectance values increased as soil moisture content decreased, with the 0% moisture model being consistently more accurate; (2) models for SOC and TN on first-derivative spectra had higher accuracy; and (3) the RF exhibited higher inversion accuracy and stability (R2 = 0.30–0.69). (4) The SHAP analysis confirmed 1865 nm and 1419 nm as the most contributory bands for SOC and TN prediction respectively, validating the RF model’s spectral interpretation capability.

Ecologies doi: 10.3390/ecologies6040074

Authors: Joel Rascón-Solano Samuel Alberto García-García Rufino Sandoval-García Eduardo Alanís-Rodríguez Sandra Pérez-Álvarez Patricia Uranga-Valencia Oscar Aguirre-Calderón Gerónimo Quiñonez-Barraza Juan Abel Nájera-Luna Benedicto Vargas-Larreta Francisco Hernández

Understanding the structural dynamics of temperate forests is essential for their sustainable management. This study assessed the vertical structure of a mixed temperate forest in the Sierra Madre Occidental, Mexico, under an individual selection cutting regime implemented in 2012 and 2022. Nine Permanent Silvicultural Research Sites were established, and measurements were carried out in 2012, 2022, and 2023 to record tree species, height, and crown cover. The analyses describe dendrometric variables, structural verticality indices and the Pretzsch index; regression models were fitted and Kruskal–Wallis tests performed. The results revealed a multistratified forest: Pinus durangensis dominates the upper canopy, while broadleaved species concentrate in the lower layers, enriching the understorey. Following silvicultural interventions, structural reorganisation was evident, with an increase in emergent individuals in the canopy and stability in crown-cover frequencies. A slight increase in pine and oak cover was detected, together with the presence of new tree species characteristic of the region. Taken together, the findings indicate that planned individual-selection cutting can maintain the stand’s original vertical structure and the functionality of the mixed temperate forest in northern Mexico, providing an analytical approach applicable to other comparable forest regions.

Ecologies doi: 10.3390/ecologies6040073

Authors: Anzhela Kolesnyk Oleksandra Kolesnyk Yurij Leno Edit Kosztyuné Krajnyák Béla Szabó Zsolt Tibor Hörcsik Zoltán Cziáky Judit Dobránszki Anikó Németh Judit Csabai

Potentilla erecta (L.) Raeusch. is a widely used medicinal species valued for its astringent, anti-inflammatory, and antimicrobial effects. This study examined the variation in hydrolysable tannin and flavonoid content in rhizomes of wild-growing populations collected along an elevational gradient in the Ukrainian Carpathians (180–2020 m above sea level). Rhizomes from fifteen populations were analyzed using pharmacopoeial methods, including thin-layer chromatography for tannins and spectrophotometry for flavonoids. Tannin levels ranged from 15.57% to 31.82%, while flavonoid contents varied between 0.23% and 0.40%, expressed as a percentage of dry weight. Both metabolites showed a strong positive correlation with altitude (r = 0.88 for tannins; r = 0.84 for flavonoids), indicating a clear influence of elevation on their accumulation. The highest concentrations were consistently found in high-mountain populations. These results suggest that environmental factors associated with increasing elevation, such as reduced temperature and enhanced ultraviolet radiation, play a significant role in shaping the phytochemical profile of P. erecta. The study contributes to the understanding of altitudinal effects on secondary metabolite accumulation in mountain plants and provides a basis for further ecological and pharmacological-oriented research related to this species.

Ecologies doi: 10.3390/ecologies6040072

Authors: Zihan Wang Yishuo Gu Meng Zhang Tianxiao Li

Advancing ecological civilization in the Yellow River Basin requires a nuanced understanding of the spatiotemporal evolution of ecosystem service value (ESV) and its underlying drivers, which are fundamental to regional sustainable development. This study examines the Henan section of the Yellow River Basin, applying the equivalent factor method to estimate ESV in 2020 at three grid scales: 3 km × 3 km, 5 km × 5 km, and 10 km × 10 km. Spatial patterns of land-averaged ESV at each scale are characterized using autocorrelation analysis, while the geodetector model is employed to identify and quantify the influence of driving factors on ESV spatial heterogeneity. The findings reveal that (1) ESV displays both consistent and variable spatial patterns, with higher values in the west and north, lower values in the east and south, and a distinct high-value belt along water bodies; (2) strong spatial positive correlation and aggregation of ESV are observed at all grid scales, though these effects weaken as grid cell size increases; and (3) human activities exert a significant influence on regional ESV, with the interaction of multiple factors providing robust explanatory power for ESV variation, which diminishes with increasing scale. These results offer insights for optimizing ecosystem management and promoting sustainable development in the Yellow River Basin.

Ecologies doi: 10.3390/ecologies6040071

Authors: Galo Pabón-Garcés Lucía Vásquez-Hernández Gladys Yaguana-Jiménez Patricia Aguirre-Mejía

Schoenoplectus californicus (Totora) is a wetland plant of cultural and ecological importance, traditionally used for handicrafts and habitat conservation in Andean lakes. This study investigates its vegetative growth in two Andean lakes in Imbabura, Ecuador (Yahuarcocha and Imbacocha), which present contrasting chemical and biological conditions (total nitrogen, total phosphorus, and chlorophyll a). Vegetative growth analysis, using indices, provides tools for understanding Totora growth dynamics within a cultivation cycle. By quantifying biomass accumulation and other parameters, it is possible to infer how the plant responds to its environment and to guide its production and management. Our objective was to evaluate how physiological and morphological traits influence growth under differential nutrient conditions. A 210-day field trial was conducted with periodic sampling and analysis of physiological indices, combining classical and functional growth approaches. Key growth indices—relative growth rate (RGR), net assimilation rate (NAR), and leaf area ratio (LAR)—were calculated from photosynthetic surface area and dry biomass. Results show that plants in Yahuarcocha, a hypertrophic lake, exhibited greater biomass production (up to 2380 g m−2) and photosynthetic area (8.68 m2), reaching peak growth at 150 days. In contrast, plants in Imbacocha, a eutrophic lake, reached maximum growth at 180 days, with a greater dependence on NAR. Strong correlations among RGR, NAR, and LAR were observed in Yahuarcocha, highlighting the influence of higher nutrient concentrations and harvesting pressure on growth dynamics. These findings underscore the importance of considering lake trophic status when planning sustainable harvesting and cultivation strategies for Totora in Andean wetlands.