MDPI Publishing

Diversity, Vol. 4, Pages 59-73: Mosses Like It Rough—Growth Form Specific Responses of Mosses, Herbaceous and Woody Plants to Micro-Relief Heterogeneity

Benjamin F. Leutner — Fri, 10 Feb 2012 00:00:00 CET

Micro-relief heterogeneity can lead to substantial variability in microclimate and hence niche opportunities on a small scale. We explored the relationship between plant species richness and small-scale heterogeneity of micro-relief on the subtropical island of La Palma, Canary Islands. Overall, we sampled 40 plots in laurel and pine forests at four altitudinal bands. Species richness was recorded separately for various growth forms (i.e., mosses, herbaceous and woody plants). Site conditions such as altitude, slope, aspect, and tree density were measured. Micro-relief heterogeneity was characterized by surface structure and a subsequently derived surface heterogeneity index. The effect of micro-relief heterogeneity on species richness was analysed by means of linear mixed effect models and variance partitioning. Effects of micro-relief heterogeneity on species richness varied considerably between growth forms. While moss richness was affected significantly by micro-relief heterogeneity, herbaceous and woody plants richness responded mainly to larger-scale site conditions such as aspect and tree density. Our results stress the importance of small-scale relief heterogeneity for the explanation of spatial patterns of species richness. This poses new challenges as small-scale heterogeneity is largely underrepresented, e.g. with regard to its application in species distribution models.

Sensors, Vol. 12, Pages 2005-2017: A Method Based on Multi-Sensor Data Fusion for Fault Detection of Planetary Gearboxes

Yaguo Lei — Fri, 10 Feb 2012 00:00:00 CET

Studies on fault detection and diagnosis of planetary gearboxes are quite limited compared with those of fixed-axis gearboxes. Different from fixed-axis gearboxes, planetary gearboxes exhibit unique behaviors, which invalidate fault diagnosis methods that work well for fixed-axis gearboxes. It is a fact that for systems as complex as planetary gearboxes, multiple sensors mounted on different locations provide complementary information on the health condition of the systems. On this basis, a fault detection method based on multi-sensor data fusion is introduced in this paper. In this method, two features developed for planetary gearboxes are used to characterize the gear health conditions, and an adaptive neuro-fuzzy inference system (ANFIS) is utilized to fuse all features from different sensors. In order to demonstrate the effectiveness of the proposed method, experiments are carried out on a planetary gearbox test rig, on which multiple accelerometers are mounted for data collection. The comparisons between the proposed method and the methods based on individual sensors show that the former achieves much higher accuracies in detecting planetary gearbox faults.

Sensors, Vol. 12, Pages 1990-2004: Robust Kernel-Based Tracking with Multiple Subtemplates in Vision Guidance System

Yuzhuang Yan — Fri, 10 Feb 2012 00:00:00 CET

The mean shift algorithm has achieved considerable success in target tracking due to its simplicity and robustness. However, the lack of spatial information may result in its failure to get high tracking precision. This might be even worse when the target is scale variant and the sequences are gray-levels. This paper presents a novel multiple subtemplates based tracking algorithm for the terminal guidance application. By applying a separate tracker to each subtemplate, it can handle more complicated situations such as rotation, scaling, and partial coverage of the target. The innovations include: (1) an optimal subtemplates selection algorithm is designed, which ensures that the selected subtemplates maximally represent the information of the entire template while having the least mutual redundancy; (2) based on the serial tracking results and the spatial constraint prior to those subtemplates, a Gaussian weighted voting method is proposed to locate the target center; (3) the optimal scale factor is determined by maximizing the voting results among the scale searching layers, which avoids the complicated threshold setting problem. Experiments on some videos with static scenes show that the proposed method greatly improves the tracking accuracy compared to the original mean shift algorithm.

IJERPH, Vol. 9, Pages 521-530: Age, Gender and Suicidal Ideation Following Voluntary HIV Counseling and Testing

Lourens Schlebusch — Fri, 10 Feb 2012 00:00:00 CET

The aim of this study was to determine the prevalence of suicidal ideation in patients who were tested for HIV-infection and whether along with their HIV status, age and gender influenced their risk for suicidal ideation. The sample consisted of 189 patients who attended a voluntary HIV counseling and testing clinic (VCT) at a general state hospital in Durban, South Africa. Their mean age at baseline was 34.2 years, with an age range of between 16–79 years. Seropositivity, age and gender were significantly associated with suicidal ideation. The majority of these patients were in the younger age group, and young males had a 1.8 times higher risk for suicidal ideation than females. Although risk factors for seropositive-related suicidal ideation can be complex and multi-factorial, this study identified a young age and male gender as important high risk factors in the sample studied. It is recommended that all, but especially young male HIV-infected patients seen at a VCT clinic be screened for suicidal ideation and that early intervention to prevent subsequent suicides or suicidal attempts be included in pre- and post-test HIV counseling.

Sensors, Vol. 12, Pages 1967-1989: Secure Cooperation of Autonomous Mobile Sensors Using an Underwater Acoustic Network

Andrea Caiti — Fri, 10 Feb 2012 00:00:00 CET

Methodologies and algorithms are presented for the secure cooperation of a team of autonomous mobile underwater sensors, connected through an acoustic communication network, within surveillance and patrolling applications. In particular, the work proposes a cooperative algorithm in which the mobile underwater sensors (installed on Autonomous Underwater Vehicles—AUVs) respond to simple local rules based on the available information to perform the mission and maintain the communication link with the network (behavioral approach). The algorithm is intrinsically robust: with loss of communication among the vehicles the coverage performance (i.e., the mission goal) is degraded but not lost. The ensuing form of graceful degradation provides also a reactive measure against Denial of Service. The cooperative algorithm relies on the fact that the available information from the other sensors, though not necessarily complete, is trustworthy. To ensure trustworthiness, a security suite has been designed, specifically oriented to the underwater scenario, and in particular with the goal of reducing the communication overhead introduced by security in terms of number and size of messages. The paper gives implementation details on the integration between the security suite and the cooperative algorithm and provides statistics on the performance of the system as collected during the UAN project sea trial held in Trondheim, Norway, in May 2011.

Sensors, Vol. 12, Pages 1950-1966: Electrical Model of a Carbon-Polymer Composite (CPC) Collision Detector

Karl Kruusamäe — Fri, 10 Feb 2012 00:00:00 CET

We present a study of an electrical model of electromechanically active carbon-polymer composite (CPC) with carbide-derived carbon (CDC) electrodes. The major focus is on investigation of surface electrode behavior upon external bending of the material. We show that electrical impedance measured from the surface of the CDC-based CPC can be used to determine the curvature of the material and, hence, the tip displacement of a CPC laminate in a cantilever configuration. It is also shown that by measuring surface signals in the process of an actuator’s work-cycle, we obtain a self-sensing collision-detecting CPC actuator that can be considered as a counterpart of biomimetic vibrissae.

Polymers, Vol. 4, Pages 486-500: Enzymatic Synthesis and Crosslinking of Novel High Molecular Weight Polyepoxyricinoleate

Ayaki Kazariya — Fri, 10 Feb 2012 00:00:00 CET

Methyl epoxyricinoleate was prepared in high yield by the lipase-catalyzed epoxidation of methyl ricinoleate with H2O2. A high molecular weight polyepoxyricinoleate (PER) with a maximum weight average molecular weight (Mw) of 272,000 was enzymatically prepared by the polycondensation of methyl epoxyricinoleate using immobilized lipase from Burkholderia cepacia (lipase PS-IM) in bulk at 80 °C for 5 d. PER showed good low temperature fluidability. PER was readily cured by maleic anhydride (MA) at 80 °C to produce a chloroform-insoluble PER-MA film. Both the glass transition temperature and Young’s modulus increased with increasing MA content and PER Mw. In contrast, the elongation at break decreased with increasing MA content and PER Mw. Methyl epoxyricinoleate, PER and PER-MA showed biodegradability by activated sludge, and that of the PER-MA film decreased with increasing MA content.

Sensors, Vol. 12, Pages 1930-1949: Sunlight Intensity Based Global Positioning System for Near-Surface Underwater Sensors

Javier V. Gómez — Fri, 10 Feb 2012 00:00:00 CET

Water monitoring is important in domains including documenting climate change, weather prediction and fishing. This paper presents a simple and energy efficient localization strategy for near surface buoy based sensors. Sensors can be dropped randomly in the ocean and thus self-calibrate in terms of geographic location such that geo-tagged observations of water quality can be made without the need for costly and energy consuming GPS-hardware. The strategy is based on nodes with an accurate clock and light sensors that can regularly sample the level of light intensity. The measurements are fitted into a celestial model of the earth motion around the sun. By identifying the trajectory of the sun across the skies one can accurately determine sunrise and sunset times, and thus extract the longitude and latitude of the sensor. Unlike previous localization techniques for underwater sensors, the current approach does not rely on stationary or mobile reference points.

Polymers, Vol. 4, Pages 463-485: Multiscale Modeling for Host-Guest Chemistry of Dendrimers in Solution

Seung Ha Kim — Fri, 10 Feb 2012 00:00:00 CET

Dendrimers have been widely used as nanostructured carriers for guest species in a variety of applications in medicine, catalysis, and environmental remediation. Theory and simulation methods are an important complement to experimental approaches that are designed to develop a fundamental understanding about how dendrimers interact with guest molecules. This review focuses on computational studies aimed at providing a better understanding of the relevant physicochemical parameters at play in the binding and release mechanisms between polyamidoamine (PAMAM) dendrimers and guest species. We highlight recent contributions that model supramolecular dendrimer-guest complexes over the temporal and spatial scales spanned by simulation methods ranging from all-atom molecular dynamics to statistical field theory. The role of solvent effects on dendrimer-guest interactions and the importance of relating model parameters across multiple scales is discussed.

Sensors, Vol. 12, Pages 1919-1929: The Effect of Direction on Cursor Moving Kinematics

Ling-Fu Meng — Fri, 10 Feb 2012 00:00:00 CET

There have been only few studies to substantiate the kinematic characteristics of cursor movement. In this study, a quantitative experimental research method was used to explore the effect of moving direction on the kinematics of cursor movement in 24 typical young persons using our previously developed computerized measuring program. The results of multiple one way repeated measures ANOVAs and post hoc LSD tests demonstrated that the moving direction had effects on average velocity, movement time, movement unit and peak velocity. Moving leftward showed better efficiency than moving rightward, upward and downward from the kinematic evidences such as velocity, movement unit and time. Moreover, the unique pattern of the power spectral density (PSD) of velocity (strategy for power application) explained why the smoothness was still maintained while moving leftward even under an unstable situation with larger momentum. Moreover, the information from this cursor moving study can guide us to relocate the toolbars and icons in the window interface, especially for individuals with physical disabilities whose performances are easily interrupted while controlling the cursor in specific directions.

Sensors, Vol. 12, Pages 1898-1918: Fiber Bragg Grating Sensors for Harsh Environments

Stephen J. Mihailov — Fri, 10 Feb 2012 00:00:00 CET

Because of their small size, passive nature, immunity to electromagnetic interference, and capability to directly measure physical parameters such as temperature and strain, fiber Bragg grating sensors have developed beyond a laboratory curiosity and are becoming a mainstream sensing technology. Recently, high temperature stable gratings based on regeneration techniques and femtosecond infrared laser processing have shown promise for use in extreme environments such as high temperature, pressure or ionizing radiation. Such gratings are ideally suited for energy production applications where there is a requirement for advanced energy system instrumentation and controls that are operable in harsh environments. This paper will present a review of some of the more recent developments.

Sensors, Vol. 12, Pages 1885-1897: Gen-2 Hand-Held Optical Imager towards Cancer Imaging: Reflectance and Transillumination Phantom Studies

Jean Gonzalez — Fri, 10 Feb 2012 00:00:00 CET

Hand-held near-infrared (NIR) optical imagers are developed by various researchers towards non-invasive clinical breast imaging. Unlike these existing imagers that can perform only reflectance imaging, a generation-2 (Gen-2) hand-held optical imager has been recently developed to perform both reflectance and transillumination imaging. The unique forked design of the hand-held probe head(s) allows for reflectance imaging (as in ultrasound) and transillumination or compressed imaging (as in X-ray mammography). Phantom studies were performed to demonstrate two-dimensional (2D) target detection via reflectance and transillumination imaging at various target depths (1–5 cm deep) and using simultaneous multiple point illumination approach. It was observed that 0.45 cc targets were detected up to 5 cm deep during transillumination, but limited to 2.5 cm deep during reflectance imaging. Additionally, implementing appropriate data post-processing techniques along with a polynomial fitting approach, to plot 2D surface contours of the detected signal, yields distinct target detectability and localization. The ability of the gen-2 imager to perform both reflectance and transillumination imaging allows its direct comparison to ultrasound and X-ray mammography results, respectively, in future clinical breast imaging studies.

Sensors, Vol. 12, Pages 1863-1884: Parametric Dense Stereovision Implementation on a System-on Chip (SoC)

Alfredo Gardel — Fri, 10 Feb 2012 00:00:00 CET

This paper proposes a novel hardware implementation of a dense recovery of stereovision 3D measurements. Traditionally 3D stereo systems have imposed the maximum number of stereo correspondences, introducing a large restriction on artificial vision algorithms. The proposed system-on-chip (SoC) provides great performance and efficiency, with a scalable architecture available for many different situations, addressing real time processing of stereo image flow. Using double buffering techniques properly combined with pipelined processing, the use of reconfigurable hardware achieves a parametrisable SoC which gives the designer the opportunity to decide its right dimension and features. The proposed architecture does not need any external memory because the processing is done as image flow arrives. Our SoC provides 3D data directly without the storage of whole stereo images. Our goal is to obtain high processing speed while maintaining the accuracy of 3D data using minimum resources. Configurable parameters may be controlled by later/parallel stages of the vision algorithm executed on an embedded processor. Considering hardware FPGA clock of 100 MHz, image flows up to 50 frames per second (fps) of dense stereo maps of more than 30,000 depth points could be obtained considering 2 Mpix images, with a minimum initial latency. The implementation of computer vision algorithms on reconfigurable hardware, explicitly low level processing, opens up the prospect of its use in autonomous systems, and they can act as a coprocessor to reconstruct 3D images with high density information in real time.

Marine Drugs, Vol. 10, Pages 349-357: Two New Tryptamine Derivatives, Leptoclinidamide and (-)-Leptoclinidamine B, from an Indonesian Ascidian Leptoclinides dubius

Hiroyuki Yamazaki — Fri, 10 Feb 2012 00:00:00 CET

Two new tryptamine-derived alkaloids, named as leptoclinidamide (1) and (-)-leptoclinidamine B (2), were isolated from an Indonesian ascidian Leptoclinides dubius together with C2-α-D-mannosylpyranosyl-L-tryptophan (3). The structure of 1 was assigned on the basis of spectroscopic data for 1 and its N-acetyl derivative (4). Compound 1 was an amide of tryptamine with two β-alanine units. Although the planar structure of 2 is identical to that of the known compound (+)-leptoclinidamine B (5), compound 2 was determined to be the enantiomer of 5 based on amino acid analysis using HPLC methods. Compounds 1 to 4 were evaluated for cytotoxicity against two human cancer cell lines, HCT-15 (colon) and Jurkat (T-cell lymphoma) cells, but none of the compounds showed activity.

IJMS, Vol. 13, Pages 1933-1950: Glucose-Modulated Mitochondria Adaptation in Tumor Cells: A Focus on ATP Synthase and Inhibitor Factor 1

Rossana Domenis — Fri, 10 Feb 2012 00:00:00 CET

Warburg’s hypothesis has been challenged by a number of studies showing that oxidative phosphorylation is repressed in some tumors, rather than being inactive per se. Thus, treatments able to shift energy metabolism by activating mitochondrial pathways have been suggested as an intriguing basis for the optimization of antitumor strategies. In this study, HepG2 hepatocarcinoma cells were cultivated with different metabolic substrates under conditions mimicking “positive” (activation/biogenesis) or “negative” (silencing) mitochondrial adaptation. In addition to the expected up-regulation of mitochondrial biogenesis, glucose deprivation caused an increase in phosphorylating respiration and a rise in the expression levels of the ATP synthase β subunit and Inhibitor Factor 1 (IF1). Hyperglycemia, on the other hand, led to a markedly decreased level of the transcriptional coactivator PGC-α suggesting down-regulation of mitochondrial biogenesis, although no change in mitochondrial mass and no impairment of phosphorylating respiration were observed. Moreover, a reduction in mitochondrial networking and in ATP synthase dimer stability was produced. No effect on β-ATP synthase expression was elicited. Notably, hyperglycemia caused an increase in IF1 expression levels, but it did not alter the amount of IF1 associated with ATP synthase. These results point to a new role of IF1 in relation to high glucose utilization by tumor cells, in addition to its well known effect upon mitochondrial ATP synthase regulation.

IJMS, Vol. 13, Pages 1919-1932: Presence of CP4-EPSPS Component in Roundup Ready Soybean-Derived Food Products

Honghong Wu — Fri, 10 Feb 2012 00:00:00 CET

With the widespread use of Roundup Ready soya (event 40-3-2) (RRS), the traceability of transgenic components, especially protein residues, in different soya-related foodstuffs has become an important issue. In this report, transgenic components in commercial soya (including RRS) protein concentrates were firstly detected by using polymerase chain reaction (PCR) and western blot. The results illustrated the different degradation patterns of the cp4-epsps gene and corresponding protein in RRS-derived protein concentrates. Furthermore, western blot was applied to investigate the single factor of food processing and the matrix on the disintegration of CP4-EPSPS protein in RRS powder and soya-derived foodstuffs, and trace the degradation patterns during the food production chain. Our results suggested that the exogenous full length of CP4-EPSPS protein in RRS powder was distinctively sensitive to various heat treatments, including heat, microwave and autoclave (especially), and only one degradation fragment (23.4 kD) of CP4-EPSPS protein was apparently observed when autoclaving was applied. By tracing the protein degradation during RRS-related products, including tofu, tou-kan, and bean curd sheets, however, four degradation fragments (42.9, 38.2, 32.2 and 23.4 kD) were displayed, suggesting that both boiling and bittern adding procedures might have extensive effects on CP4-EPSPS protein degradation. Our results thus confirmed that the distinctive residues of the CP4-EPSPS component could be traced in RRS-related foodstuffs.

IJMS, Vol. 13, Pages 1886-1918: The mTOR Signalling Pathway in Human Cancer

Helena Pópulo — Fri, 10 Feb 2012 00:00:00 CET

The conserved serine/threonine kinase mTOR (the mammalian target of rapamycin), a downstream effector of the PI3K/AKT pathway, forms two distinct multiprotein complexes: mTORC1 and mTORC2. mTORC1 is sensitive to rapamycin, activates S6K1 and 4EBP1, which are involved in mRNA translation. It is activated by diverse stimuli, such as growth factors, nutrients, energy and stress signals, and essential signalling pathways, such as PI3K, MAPK and AMPK, in order to control cell growth, proliferation and survival. mTORC2 is considered resistant to rapamycin and is generally insensitive to nutrients and energy signals. It activates PKC-α and AKT and regulates the actin cytoskeleton. Deregulation of multiple elements of the mTOR pathway (PI3K amplification/mutation, PTEN loss of function, AKT overexpression, and S6K1, 4EBP1 and eIF4E overexpression) has been reported in many types of cancers, particularly in melanoma, where alterations in major components of the mTOR pathway were reported to have significant effects on tumour progression. Therefore, mTOR is an appealing therapeutic target and mTOR inhibitors, including the rapamycin analogues deforolimus, everolimus and temsirolimus, are submitted to clinical trials for treating multiple cancers, alone or in combination with inhibitors of other pathways. Importantly, temsirolimus and everolimus were recently approved by the FDA for the treatment of renal cell carcinoma, PNET and giant cell astrocytoma. Small molecules that inhibit mTOR kinase activity and dual PI3K-mTOR inhibitors are also being developed. In this review, we aim to survey relevant research, the molecular mechanisms of signalling, including upstream activation and downstream effectors, and the role of mTOR in cancer, mainly in melanoma.

Molecules, Vol. 17, Pages 1809-1818: Pressurized Hot Ethanol Extraction of Carotenoids from Carrot By-Products

Arwa Mustafa — Fri, 10 Feb 2012 00:00:00 CET

Carotenoids are known for their antioxidant activity and health promoting effects. One of the richest sources of carotenoids are carrots. However, about 25% of the annual production is regarded as by-products due to strict market policies. The aim of this study was to extract carotenoids from those by-products. Conventional carotenoid extraction methods require the use of organic solvents, which are costly, environmentally hazardous, and require expensive disposal procedures. Pressurized liquid extraction (PLE) utilizes conventional solvents at elevated temperatures and pressure, and it requires less solvent and shorter extraction times. The extraction solvent of choice in this study was ethanol, which is a solvent generally recognized as safe (GRAS). The extraction procedure was optimized by varying the extraction time (2–10 min) and the temperature (60–180 °C). β-Carotene was used as an indicator for carotenoids content in the carrots. The results showed that time and temperatures of extraction have significant effect on the yield of carotenoids. Increasing the flush volume during extraction did not improve the extractability of carotenoids, indicating that the extrication method was mainly desorption/diffusion controlled. Use of a dispersing agent that absorbs the moisture content was important for the efficiency of extraction. Analysing the content of β-carotene at the different length of extraction cycles showed that about 80% was recovered after around 20 min of extraction.

Molecules, Vol. 17, Pages 1797-1808: Phenolic Compounds and Antioxidant Activities of Liriope muscari

Wen Jie Li — Fri, 10 Feb 2012 00:00:00 CET

Five phenolic compounds, namely N-trans-coumaroyltyramine (1), N-trans-feruloyltyramine (2), N-trans-feruloyloctopamine (3), 5,7-dihydroxy-8-methoxyflavone (4) and (3S)3,5,4′-trihydroxy-7-methoxy-6-methylhomoisoflavanone (5), were isolated from the fibrous roots of Liriope muscari (Liliaceae). Compounds 2–5 were isolated for the first time from the Liriope genus. Their in vitro antioxidant activities were assessed by the DPPH and ABTS scavenging methods with microplate assays. The structure-activity relationships of compounds 1–3 are discussed.

Molecules, Vol. 17, Pages 1795-1796: Molecules Best Paper Award 2012

Derek J. McPhee — Fri, 10 Feb 2012 00:00:00 CET

Molecules start to institute the “Best Paper” award to recognize these outstanding papers in the area of natural products, medicinal chemistry and molecular diversity published in Molecules. We are pleased to announce the first “Molecules Best Paper Award” for 2012. Nominations were selected by the editor-in-chief and selected editorial board members from all the papers published in 2008. [...]

Molecules, Vol. 17, Pages 1764-1794: Applications of Supercritical Fluid Extraction (SFE) of Palm Oil and Oil from Natural Sources

Mohammed Jahurul Haque Akanda — Fri, 10 Feb 2012 00:00:00 CET

Supercritical fluid extraction (SFE), which has received much interest in its use and further development for industrial applications, is a method that offers some advantages over conventional methods, especially for the palm oil industry. SC-CO2 refers to supercritical fluid extraction (SFE) that uses carbon dioxide (CO2) as a solvent which is a nontoxic, inexpensive, nonflammable, and nonpolluting supercritical fluid solvent for the extraction of natural products. Almost 100% oil can be extracted and it is regarded as safe, with organic solvent-free extracts having superior organoleptic profiles. The palm oil industry is one of the major industries in Malaysia that provides a major contribution to the national income. Malaysia is the second largest palm oil and palm kernel oil producer in the World. This paper reviews advances in applications of supercritical carbon dioxide (SC-CO2) extraction of oils from natural sources, in particular palm oil, minor constituents in palm oil, producing fractionated, refined, bleached, and deodorized palm oil, palm kernel oil and purified fatty acid fractions commendable for downstream uses as in toiletries and confectionaries.

Polymers, Vol. 4, Pages 448-462: In Situ and Ex Situ Syntheses of Magnetic Liquid Crystalline Materials: A Comparison

Ophelie Riou — Fri, 10 Feb 2012 00:00:00 CET

Magnetic hybrid liquid crystalline composites have been obtained either by thermal decomposition of a cobalt precursor in a solution containing a liquid crystal polymer or by dispersing preformed cobalt nanorods in a liquid crystal polymer matrix. The final materials are all mesomorphous and ferromagnetic. Their magnetic characteristics are compared as a function of the synthesis method.

IJMS, Vol. 13, Pages 1858-1885: Yeast Mitochondrial Interactosome Model: Metabolon Membrane Proteins Complex Involved in the Channeling of ADP/ATP

Benjamin Clémençon — Fri, 10 Feb 2012 00:00:00 CET

The existence of a mitochondrial interactosome (MI) has been currently well established in mammalian cells but the exact composition of this super-complex is not precisely known, and its organization seems to be different from that in yeast. One major difference is the absence of mitochondrial creatine kinase (MtCK) in yeast, unlike that described in the organization model of MI, especially in cardiac, skeletal muscle and brain cells. The aim of this review is to provide a detailed description of different partner proteins involved in the synergistic ADP/ATP transport across the mitochondrial membranes in the yeast Saccharomyces cerevisiae and to propose a new mitochondrial interactosome model. The ADP/ATP (Aacp) and inorganic phosphate (PiC) carriers as well as the VDAC (or mitochondrial porin) catalyze the import and export of ADP, ATP and Pi across the mitochondrial membranes. Aacp and PiC, which appear to be associated with the ATP synthase, consist of two nanomotors (F0, F1) under specific conditions and form ATP synthasome. Identification and characterization of such a complex were described for the first time by Pedersen and co-workers in 2003.

Entropy, Vol. 14, Pages 233-251: Scientific Élan Vital: Entropy Deficit or Inhomogeneity as a Unified Concept of Driving Forces of Life in Hierarchical Biosphere Driven by Photosynthesis

Naoki Sato — Fri, 10 Feb 2012 00:00:00 CET

Life is considered something different from non-living things, but no single driving force can account for all the different aspects of life, which consists of different levels of hierarchy, such as metabolism, cell physiology, multi-cellular development and organization, population dynamics, ecosystem, and evolution. Although free energy is evidently the driving force in biochemical reactions, there is no established relationship between metabolic energy and spatiotemporal organization of living organisms, or between metabolic energy and genetic information. Since Schrödinger pointed out the importance of exporting entropy in maintaining life, misunderstandings of entropy notion have been obstacles in constructing a unified view on the driving forces of life. Here I present a simplified conceptual framework for unifying driving forces of life at various different levels of hierarchy. The key concept is “entropy deficit”, or simply, ‘inhomogeneity’, which is defined as the difference of maximal possible entropy and actual entropy. This is equivalent to information content in genetic information and protein structure, and is also defined similarly for non-homogeneous structures in ecosystems and evolution. Entropy deficit or inhomogeneoity is a unified measure of all driving forces of life, which could be considered a scientific equivalent to ‘élan vital’ of Bergson.

Molecules, Vol. 17, Pages 1751-1763: Design, Synthesis and Preliminary Pharmacological Evaluation of New Non-Steroidal Anti-Inflammatory Agents Having a 4-(Methylsulfonyl) Aniline Pharmacophore

Monther Faisel Mahdi — Fri, 10 Feb 2012 00:00:00 CET

A series of 4-(methylsulfonyl)aniline derivatives were synthesized in order to obtain new compounds as a potential anti-inflammatory agents with expected selectivity against COX-2 enzyme. In vivo acute anti-inflammatory activity of the final compounds 11–14 was evaluated in rat using an egg-white induced edema model of inflammation in a dose equivalent to 3 mg/Kg of diclofenac sodium. All tested compounds produced significant reduction of paw edema with respect to the effect of propylene glycol 50% v/v (control group). Moreover, the activity of compounds 11 and 14 was significantly higher than that of diclofenac sodium (at 3 mg/Kg) in the 120–300 minute time interval, while compound 12 expressed a comparable effect to that of diclofenac sodium in the 60–240 minute time interval time, and compound 13 showed a comparable effect to that of diclofenac sodium at all experimental times. The result of this study indicates that the incorporation of the 4-(methylsulfonyl)aniline pharamacophore into naproxen, indomethacine, diclofenac and mefanamic acid maintained their anti-inflammatory activity and may increase selectivity towards the COX-2 enzyme which will be confirmed in the future by assessing COX-2: COX-1 inhibitory ratios using a whole blood assay.

Entropy, Vol. 14, Pages 213-232: Quantitative Comparison of Conformational Ensembles

Kevin C. Wolfe — Fri, 10 Feb 2012 00:00:00 CET

A number of measures have been used in the structural biology literature to compare the shapes or conformations of biological macromolecules. However, the issue of how to compare two ensembles of conformations has received far less attention. Herein, the problem of how to quantitatively compare two such ensembles is addressed in several different ways using concepts from probability and information theory. Ultimately, such metrics could be used in the evaluation of structure-prediction algorithms and the analysis of how conformational mobility is inhibited by bound ligands.

Insects, Vol. 3, Pages 161-199: Insect Sex Determination Manipulated by Their Endosymbionts: Incidences, Mechanisms and Implications

Daisuke Kageyama — Fri, 10 Feb 2012 00:00:00 CET

The sex-determining systems of arthropods are surprisingly diverse. Some species have male or female heterogametic sex chromosomes while other species do not have sex chromosomes. Most species are diploids but some species, including wasps, ants, thrips and mites, are haplodiploids (n in males; 2n in females). Many of the sexual aberrations, such as sexual mosaics, sex-specific lethality and conversion of sexuality, can be explained by developmental defects including double fertilization of a binucleate egg, loss of a sex chromosome or perturbation of sex-determining gene expression, which occur accidentally or are induced by certain environmental conditions. However, recent studies have revealed that such sexual aberrations can be caused by various groups of vertically-transmitted endosymbiotic microbes such as bacteria of the genera Wolbachia, Rickettsia, Arsenophonus, Spiroplasma and Cardinium, as well as microsporidian protists. In this review, we first summarize the accumulated data on endosymbiont-induced sexual aberrations, and then discuss how such endosymbionts affect the developmental system of their hosts and what kinds of ecological and evolutionary effects these endosymbionts have on their host populations.

Insects, Vol. 3, Pages 141-160: Phylogenetic Analysis of Fusarium solani Associated with the Asian Longhorned Beetle, Anoplophora glabripennis

Scott M. Geib — Fri, 10 Feb 2012 00:00:00 CET

Culture-independent analysis of the gut of a wood-boring insect, Anoplophora glabripennis (Coleoptera: Cerambycidae), revealed a consistent association between members of the fungal Fusarium solani species complex and the larval stage of both colony-derived and wild A. glabripennis populations. Using the translation elongation factor 1-alpha region for culture-independent phylogenetic and operational taxonomic unit (OTU)-based analyses, only two OTUs were detected, suggesting that genetic variance at this locus was low among A. glabripennis-associated isolates. To better survey the genetic variation of F. solani associated with A. glabripennis, and establish its phylogenetic relationship with other members of the F. solani species complex, single spore isolates were created from different populations and multi-locus phylogenetic analysis was performed using a combination of the translation elongation factor alpha-1, internal transcribed spacer, and large subunit rDNA regions. These analyses revealed that colony-derived larvae reared in three different tree species or on artificial diet, as well as larvae from wild populations collected from three additional tree species in New York City and from a single tree species in Worcester, MA, consistently harbored F. solani within their guts. While there is some genetic variation in the F. solani carried between populations, within-population variation is low. We speculate that F. solani is able to fill a broad niche in the A. glabripennis gut, providing it with fungal lignocellulases to allow the larvae to grow and develop on woody tissue. However, it is likely that many F. solani genotypes could potentially fill this niche, so the relationship may not be limited to a single member of the F. solani species complex. While little is known about the role of filamentous fungi and their symbiotic associations with insects, this report suggests that larval A. glabripennis has developed an intimate relationship with F. solani that is not limited by geographic location or host tree.

Insects, Vol. 3, Pages 133-140: Near-Optimal Foraging in the Pacific Cicada Killer Sphecius convallis Patton (Hymenoptera: Crabronidae)

Joseph R. Coelho — Fri, 10 Feb 2012 00:00:00 CET

This study evaluated foraging effectiveness of Pacific cicada killers (Sphecius convallis) by comparing observed prey loads to that predicted by an optimality model. Female S. convallis preyed exclusively on the cicada Tibicen parallelus, resulting in a mean loaded flight muscle ratio (FMR) of 0.187 (N = 46). This value lies just above the marginal level, and only seven wasps (15%) were below 0.179. The low standard error (0.002) suggests that S. convallis is the most ideal flying predator so far examined in this respect. Preying on a single species may have allowed stabilizing selection to adjust the morphology of females to a nearly ideal size. That the loaded FMR is slightly above the marginal level may provide a small safety factor for wasps that do not have optimal thorax temperatures or that have to contend with attempted prey theft. Operational FMR was directly related to wasp body mass. Smaller wasps were overloaded in spite of provisioning with smaller cicadas, while larger wasps were underloaded despite provisioning with larger cicadas. Small wasps may have abandoned larger cicadas because of difficulty with carriage.

Genes, Vol. 3, Pages 115-137: Genomic Distribution and Divergence of Levansucrase-Coding Genes in Pseudomonas syringae

Abhishek Srivastava — Fri, 10 Feb 2012 00:00:00 CET

In the plant pathogenic bacterium, Pseudomonas syringae, the exopolysaccharide levan is synthesized by extracellular levansucrase (Lsc), which is encoded by two conserved 1,296-bp genes termed lscB and lscC in P. syringae strain PG4180. A third gene, lscA, is homologous to the 1,248-bp lsc gene of the bacterium Erwinia amylovora, causing fire blight. However, lscA is not expressed in P. syringae strain PG4180. Herein, PG4180 lscA was shown to be expressed from its native promoter in the Lsc-deficient E. amylovora mutant, Ea7/74-LS6, suggesting that lscA might be closely related to the E. amylovora lsc gene. Nucleotide sequence analysis revealed that lscB and lscC homologs in several P. syringae strains are part of a highly conserved 1.8-kb region containing the ORF, flanked by 450-452-bp and 49-51-bp up- and downstream sequences, respectively. Interestingly, the 450-452-bp upstream sequence, along with the initial 48-bp ORF sequence encoding for the N-terminal 16 amino acid residues of Lsc, were found to be highly similar to the respective sequence of a putatively prophage-borne glycosyl hydrolase-encoding gene in several P. syringae genomes. Minimal promoter regions of lscB and lscC were mapped in PG4180 by deletion analysis and were found to be located in similar positions upstream of lsc genes in three P. syringae genomes. Thus, a putative 498-500-bp promoter element was identified, which possesses the prophage-associated com gene and DNA encoding common N-terminal sequences of all 1,296-bp Lsc and two glycosyl hydrolases. Since the gene product of the non-expressed 1,248-bp lscA is lacking this conserved N-terminal region but is otherwise highly homologous to those of lscB and lscC, it was concluded that lscA might have been the ancestral lsc gene in E. amylovora and P. syringae. Our data indicated that its highly expressed paralogs in P. syringae are probably derived from subsequent recombination events initiated by insertion of the 498-500-bp promoter element, described herein, containing a translational start site.

Agronomy, Vol. 2, Pages 1-13: Wheat Nitrogen Fertilisation Effects on the Performance of the Cereal Aphid Metopolophium dirhodum

Alan F. J. Gash — Thu, 09 Feb 2012 00:00:00 CET

The effects of five rates of nitrogen fertiliser applications on the performance of the cereal aphid Metopolophium dirhodum on winter wheat, within the range of rates recommended for UK crops, were investigated over two seasons in field-grown crops and also on plants grown in the glasshouse. Longevity was unaffected by the level of fertilisation, but aphid intrinsic rate of increase and fecundity increased with each level applied. In the second field season, when a higher upper limit was used, many of these increases were significant. A previously unreported finding for this species was that there was a significant decrease in fecundity for the highest rate of fertilisation. Results for the glasshouse-reared aphids followed a similar pattern to those in the field, and overall they underline recent reports in the literature of the negative effects of high nutrient concentrations on the performance of herbivorous insects. The underlying reasons for these are discussed.

Sustainability, Vol. 4, Pages 244-256: Bioenergy Villages and Regions in Germany: An Interview Study with Initiators of Communal Bioenergy Projects on the Success Factors for Restructuring the Energy Supply of the Community

André Wüste — Thu, 09 Feb 2012 00:00:00 CET

Because of the serious problems related to an energy supply based mainly on fossil and nuclear fuels, the development of renewable energy sources is urgently needed. In Germany, many villages and communities take energy production into their own hands, following the principle of a community-related energy supply. Today, approximately 50 villages or communities in Germany have restructured their energy consumption patterns to rely primarily on locally available renewable energy sources for their electricity and heat. This article describes a qualitative interview study concerning the success factors for the implementation of bioenergy villages. The interviews were conducted with 25 individuals who initiated the restructuring of energy production in their villages toward bioenergy and other renewable energy sources, such as solar or wind energy.

Remote Sensing, Vol. 4, Pages 456-483: How Robust Are Burn Severity Indices When Applied in a New Region? Evaluation of Alternate Field-Based and Remote-Sensing Methods

C. Alina Cansler — Thu, 09 Feb 2012 00:00:00 CET

Remotely sensed indices of burn severity are now commonly used by researchers and land managers to assess fire effects, but their relationship to field-based assessments of burn severity has been evaluated only in a few ecosystems. This analysis illustrates two cases in which methodological refinements to field-based and remotely sensed indices of burn severity developed in one location did not show the same improvement when used in a new location. We evaluated three methods of assessing burn severity in the field: the Composite Burn Index (CBI)—a standardized method of assessing burn severity that combines ecologically significant variables related to burn severity into one numeric site index—and two modifications of the CBI that weight the plot CBI score by the percentage cover of each stratum. Unexpectedly, models using the CBI had higher R2 and better classification accuracy than models using the weighted versions of the CBI. We suggest that the weighted versions of the CBI have lower accuracies because weighting by percentage cover decreases the influence of the dominant tree stratum, which should have the strongest relationship to optically sensed reflectance, and increases the influence of the substrates strata, which should have the weakest relationship with optically sensed reflectance in forested ecosystems. Using a large data set of CBI plots (n = 251) from four fires and CBI scores derived from additional field-based assessments of burn severity (n = 388), we predicted two metrics of image-based burn severity, the Relative differenced Normalized Burn Ratio (RdNBR) and the differenced Normalized Burn Ratio (dNBR). Predictive models for RdNBR showed slightly better classification accuracy than for dNBR (overall accuracy = 62%, Kappa = 0.40, and overall accuracy = 59%, Kappa= 0.36, respectively), whereas dNBR had slightly better explanatory power, but strong differences were not apparent. RdNBR may provide little or no improvement over dNBR in systems where pre-fire reflectance is not highly variable, but may be more appropriate for comparing burn severity among regions.

Sensors, Vol. 12, Pages 1846-1862: Extraction and Analysis of Impervious Surfaces Based on a Spectral Un-Mixing Method Using Pearl River Delta of China Landsat TM/ETM+ Imagery from 1998 to 2008

Yingbin Deng — Thu, 09 Feb 2012 00:00:00 CET

Impervious surface area (ISA) is considered as an indicator of environment change and is regarded as an important input parameter for hydrological cycle simulation, water management and area pollution assessment. The Pearl River Delta (PRD), the 3rd most important economic district of China, is chosen in this paper to extract the ISA information based on Landsat images of 1998, 2003 and 2008 by using a linear spectral un-mixing method and to monitor impervious surface change by analyzing the multi-temporal Landsat-derived fractional impervious surface. Results of this study were as follows: (1) the area of ISA in the PRD increased 79.09% from 1998 to 2003 and 26.88% from 2003 to 2008 separately; (2) the spatial distribution of ISA was described according to the 1998/2003 percentage respectively. Most of middle and high percentage ISA was located in northwestern and southeastern of the whole delta, and middle percentage ISA was mainly located in the city interior, high percentage ISA was mainly located in the suburban around the city accordingly; (3) the expanding direction and trend of high percentage ISA was discussed in order to understand the change of urban in this delta; High percentage ISA moved from inner city to edge of urban area during 1998–2003 and moved to the suburban area that far from the urban area mixed with jumpily and gradually during 2003–2008. According to the discussion of high percentage ISA spatial expanded direction, it could be found out that high percentage ISA moved outward from the centre line of Pearl River of the whole delta while a high ISA percentage in both shores of the Pearl River Estuary moved toward the Pearl River; (4) combining the change of ISA with social conditions, the driving relationship was analyzed in detail. It was evident that ISA percentage change had a deep relationship with the economic development of this region in the past ten years. Contemporaneous major sport events (16th Asia Games of Guangzhou, 26th Summer Universidad of Shenzhen) and the government policies also promoted the development of the ISA. Meanwhile, topographical features like the National Nature Reserve of China restricted and affected the expansion of the ISA. Above all, this paper attempted to extract ISA in a major region of the PRD; the temporal and spatial analyses to PRD ISA demonstrated the drastic changes in developed areas of China. These results were important and valuable for land use management, ecological protection and policy establishment.

Sensors, Vol. 12, Pages 1827-1845: AURP: An AUV-Aided Underwater Routing Protocol for Underwater Acoustic Sensor Networks

Seokhoon Yoon — Thu, 09 Feb 2012 00:00:00 CET

Deploying a multi-hop underwater acoustic sensor network (UASN) in a large area brings about new challenges in reliable data transmissions and survivability of network due to the limited underwater communication range/bandwidth and the limited energy of underwater sensor nodes. In order to address those challenges and achieve the objectives of maximization of data delivery ratio and minimization of energy consumption of underwater sensor nodes, this paper proposes a new underwater routing scheme, namely AURP (AUV-aided underwater routing protocol), which uses not only heterogeneous acoustic communication channels but also controlled mobility of multiple autonomous underwater vehicles (AUVs). In AURP, the total data transmissions are minimized by using AUVs as relay nodes, which collect sensed data from gateway nodes and then forward to the sink. Moreover, controlled mobility of AUVs makes it possible to apply a short-range high data rate underwater channel for transmissions of a large amount of data. To the best to our knowledge, this work is the first attempt to employ multiple AUVs as relay nodes in a multi-hop UASN to improve the network performance in terms of data delivery ratio and energy consumption. Simulations, which are incorporated with a realistic underwater acoustic communication channel model, are carried out to evaluate the performance of the proposed scheme, and the results indicate that a high delivery ratio and low energy consumption can be achieved.

Sensors, Vol. 12, Pages 1816-1826: Evaluation of Electrical and Optical Plethysmography Sensors for Noninvasive Monitoring of Hemoglobin Concentration

Justin P. Phillips — Thu, 09 Feb 2012 00:00:00 CET

Completely noninvasive monitoring of hemoglobin concentration has not yet been fully realized in the clinical setting. This study investigates the viability of measuring hemoglobin concentration noninvasively by evaluating the performance of two types of sensor using a tissue phantom perfused with a blood substitute. An electrical sensor designed to measure blood volume changes during the cardiac cycle was used together with an infrared optical sensor for detection of erythrocyte-bound hemoglobin. Both sensors demonstrated sensitivity to changes in pulse volume (plethysmography). The electrical sensor produced a signal referred to as capacitance plethysmograph (CPG) a quantity which was invariant to the concentration of an infrared absorbing dye present in the blood substitute. The optical sensor signal (photoplethysmograph) increased in amplitude with increasing absorber concentration. The ratio PPG:CPG is invariant to pulse pressure. This quantity is discussed as a possible index of in vivo hemoglobin concentration.

Sensors, Vol. 12, Pages 1800-1815: A Platform for Combined DNA and Protein Microarrays Based on Total Internal Reflection Fluorescence

Alexander Asanov — Thu, 09 Feb 2012 00:00:00 CET

We have developed a novel microarray technology based on total internal reflection fluorescence (TIRF) in combination with DNA and protein bioassays immobilized at the TIRF surface. Unlike conventional microarrays that exhibit reduced signal-to-background ratio, require several stages of incubation, rinsing and stringency control, and measure only end-point results, our TIRF microarray technology provides several orders of magnitude better signal-to-background ratio, performs analysis rapidly in one step, and measures the entire course of association and dissociation kinetics between target DNA and protein molecules and the bioassays. In many practical cases detection of only DNA or protein markers alone does not provide the necessary accuracy for diagnosing a disease or detecting a pathogen. Here we describe TIRF microarrays that detect DNA and protein markers simultaneously, which reduces the probabilities of false responses. Supersensitive and multiplexed TIRF DNA and protein microarray technology may provide a platform for accurate diagnosis or enhanced research studies. Our TIRF microarray system can be mounted on upright or inverted microscopes or interfaced directly with CCD cameras equipped with a single objective, facilitating the development of portable devices. As proof-of-concept we applied TIRF microarrays for detecting molecular markers from Bacillus anthracis, the pathogen responsible for anthrax.

Sensors, Vol. 12, Pages 1771-1799: Vector Disparity Sensor with Vergence Control for Active Vision Systems

Francisco Barranco — Thu, 09 Feb 2012 00:00:00 CET

This paper presents an architecture for computing vector disparity for active vision systems as used on robotics applications. The control of the vergence angle of a binocular system allows us to efficiently explore dynamic environments, but requires a generalization of the disparity computation with respect to a static camera setup, where the disparity is strictly 1-D after the image rectification. The interaction between vision and motor control allows us to develop an active sensor that achieves high accuracy of the disparity computation around the fixation point, and fast reaction time for the vergence control. In this contribution, we address the development of a real-time architecture for vector disparity computation using an FPGA device. We implement the disparity unit and the control module for vergence, version, and tilt to determine the fixation point. In addition, two on-chip different alternatives for the vector disparity engines are discussed based on the luminance (gradient-based) and phase information of the binocular images. The multiscale versions of these engines are able to estimate the vector disparity up to 32 fps on VGA resolution images with very good accuracy as shown using benchmark sequences with known ground-truth. The performances in terms of frame-rate, resource utilization, and accuracy of the presented approaches are discussed. On the basis of these results, our study indicates that the gradient-based approach leads to the best trade-off choice for the integration with the active vision system.

Sensors, Vol. 12, Pages 1758-1770: Comparison between Conduction and Convection Effects on Self-Heating in Doped Microcantilevers

Mohd Zahid Ansari — Thu, 09 Feb 2012 00:00:00 CET

The present study investigates the effects of thermal conduction and convection on self-heating temperatures and bimetallic deflections produced in doped microcantilever sensors. These cantilevers are commonly used as sensors and actuators in microsystems. The cantilever is a monolith, multi-layer structure with a thin U-shaped element inside. The cantilever substrate is made of silicon and silicon dioxide, respectively, and the element is p-doped silicon. A numerical analysis package (ANSYS) is used to study the effect of cantilever substrate material, element width, applied voltage and the operating environments on cantilever characteristics. The numerical results for temperature are compared against their analytical models. Results indicate the numerical results are accurate within 6% of analytical, and Si/Si cantilevers are more suitable for biosensors and AFM, whereas, Si/SiO2 are for hotplates and actuators applications.

Sensors, Vol. 12, Pages 1738-1757: Finger Vein Recognition Based on a Personalized Best Bit Map

Gongping Yang — Thu, 09 Feb 2012 00:00:00 CET

Finger vein patterns have recently been recognized as an effective biometric identifier. In this paper, we propose a finger vein recognition method based on a personalized best bit map (PBBM). Our method is rooted in a local binary pattern based method and then inclined to use the best bits only for matching. We first present the concept of PBBM and the generating algorithm. Then we propose the finger vein recognition framework, which consists of preprocessing, feature extraction, and matching. Finally, we design extensive experiments to evaluate the effectiveness of our proposal. Experimental results show that PBBM achieves not only better performance, but also high robustness and reliability. In addition, PBBM can be used as a general framework for binary pattern based recognition.

Molecules, Vol. 17, Pages 1744-1750: The Incubation of 13a,17-Dihydroxystemodane with Cephalosporium aphidicola

Braulio M. Fraga — Thu, 09 Feb 2012 00:00:00 CET

The biotransformation of 13a,17-dihydroxystemodane (3) with the fungus Cephalosporium aphidicola afforded 13a,17,18-trihydroxystemodane (4), 3b,13a,17-tri-hydroxystemodane (5), 13a,17-dihydroxy-stemodan-18-oic acid (6), 3b,11β,13a,17-tetra-hydroxystemodane (7), 11β,13a,17,18-tetrahydroxystemodane (8) and 3b,13a,17,18-tetra-hydroxystemodane (9). The hydroxylation at C-18 of the substrate points to a biosynthetically-directed transformation, because aphidicolin (2) is hydroxylated at this carbon. However, the C-3(b) and C-11(b) hydroxylations seem to indicate a xenobiotic biotransformation.

Molecules, Vol. 17, Pages 1716-1743: Chemisorption and Reactions of Small Molecules on Small Gold Particles

Geoffrey C. Bond — Thu, 09 Feb 2012 00:00:00 CET

The activity of supported gold particles for a number of oxidations and hydrogenations starts to increase dramatically as the size falls below ~3 nm. This is accompanied by an increased propensity to chemisorption, especially of oxygen and hydrogen. The explanation for these phenomena has to be sought in kinetic analysis that connects catalytic activity with the strength and extent of chemisorption of the reactants, the latter depending on the electronic structure of the gold atoms constituting the active centre. Examination of the changes to the utilisation of electrons as particle size is decreased points to loss of metallic character at about 3 nm, as energy bands are replaced by levels, and a band gap appears. Detailed consideration of the Arrhenius parameters (E and ln A) for CO oxidation points clearly to a step-change in activity at the point where metallic character is lost, as opposed to there being a monotonic dependence of rate on a physical property such as the fraction of atoms at corners or edges of particles. The deplorable scarcity of kinetic information on other reactions makes extension of this analysis difficult, but non-metallic behaviour is an unavoidable property of very small gold particles, and therefore cannot be ignored when seeking to explain their exceptional activity.

Molecules, Vol. 17, Pages 1698-1715: Ergosterol-Induced Sesquiterpenoid Synthesis in Tobacco Cells

Fidele Tugizimana — Thu, 09 Feb 2012 00:00:00 CET

Plants have the ability to continuously respond to microbial signals in their environment. One of these stimuli is a steroid from fungal membranes, ergosterol, which does not occur in plants, but acts as a pathogen-associated molecular pattern molecule to trigger defence mechanisms. Here we investigated the effect of ergosterol on the secondary metabolites in tobacco (Nicotiana tabacum) cells by profiling the induced sesquiterpenoids. Suspensions of tobacco cells were treated with different concentrations (0–1,000 nM) of ergosterol and incubated for different time periods (0–24 h). Metabolites were extracted with a selective dispersive liquid-liquid micro-extraction method. Thin layer chromatography was used as a screening method for identification of sesquiterpenoids in tobacco extracts. Liquid chromatography coupled to mass spectrometry was used for quantitative and qualitative analyses. The results showed that ergosterol triggered differential changes in the metabolome of tobacco cells, leading to variation in the biosynthesis of secondary metabolites. Metabolomic analysis through principal component analysis-scores plots revealed clusters of sample replicates for ergosterol treatments of 0, 50, 150, 300 and 1,000 nM and time-dependent variation at 0, 6, 12, 18 and 24 h. Five bicyclic sesquiterpenoid phytoalexins, capsidiol, lubimin, rishitin, solavetivone and phytuberin, were identified as being ergosterol-induced, contributing to the altered metabolome.

Polymers, Vol. 4, Pages 408-447: Polysilane Dendrimers

Clemens Krempner — Thu, 09 Feb 2012 00:00:00 CET

The synthesis, structure and electronic properties of polysilane dendrimers, a relatively new class of highly branched and silicon-rich molecular architectures is reviewed. After a detailed discussion of main synthetic strategies to well-defined single-core and double-core polysilane dendrimers, important structural and conformational features determined by single crystal X-ray crystallography and 29Si-NMR spectroscopy are presented. The last part highlights the most interesting photochemical properties of polysilane dendrimers such as UV absorption and emission behavior, which are compared with those of linear and branched polysilanes.

IJMS, Vol. 13, Pages 1846-1857: The -308G/A of Tumor Necrosis Factor (TNF)-α and 825C/T of Guanidine Nucleotide Binding Protein 3 (GNB3) are Associated with the Onset of Acute Myocardial Infarction and Obesity in Taiwan

Wei-To Chang — Thu, 09 Feb 2012 00:00:00 CET

Acute myocardial infarction is a highly prevalent cardiovascular disease in Taiwan. Among several etiological risk factors, obesity and inflammation are strongly associated with the frequency of hypertension, cardiovascular disease, diabetes, and myocardial infarction. To discriminate obesity- and inflammation-related genes and the onset of acute myocardial infarction (AMI), a case-control study was conducted to investigate the association of the -308G/A polymorphisms of tumor necrosis factor (TNF)-α and the C825T polymorphism of guanidine nucleotide binding protein 3 (GNB3) with the onset of AMI among Taiwanese cohorts. A total of 103 AMI patients and 163 matched normal control samples were enrolled in the present study. The genomic DNA was extracted and subjected into polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis. An association between the A homozygosity of the TNF-α-308G/A polymorphism and the onset of AMI was observed among the male subjects (p = 0.026; Spearman index = 0.200, p = 0.008). An association between the T homozygosity of GNB3 C825T polymorphism and obesity was also observed (Fisher’s exact, p = 0.009). The TT genotype has a protective effect against acquiring AMI among the obese female population in Taiwan (Fisher’s exact, p = 0.032). In conclusion, TNF-α-308G/A and the GNB3 C825T polymorphisms are associated with obesity and AMI in the Taiwanese population.

IJMS, Vol. 13, Pages 1832-1845: Isolation and Characterization of Activators of ERK/MAPK from Citrus Plants

Yoshiko Furukawa — Thu, 09 Feb 2012 00:00:00 CET

Extracellular signal-regulated kinases 1/2 (ERK1/2), components of the mitogen-activated protein kinase (MAPK) signaling cascade, have been recently shown to be involved in synaptic plasticity and in the development of long-term memory in the central nervous system (CNS). We therefore examined the ability of Citrus compounds to activate ERK1/2 in cultured rat cortical neurons, whose activation might have a protective effect against neurodegenerative neurological disorders. Among the samples tested, extracts prepared from the peels of Citrus grandis (Kawachi bankan) were found to have the greatest ability to activate ERK1/2. The active substances were isolated by chromatographic separation, and one of them was identified to be 3,5,6,7,8,3′,4′-heptamethoxyflavone (HMF). HMF significantly induced the phosphorylation of cAMP response element-binding protein (CREB), a downstream target of activated ERK1/2, which appears to be a critical step in the signaling cascade for the structural changes underlying the development of long-term potentiation (LTP). In addition, the administration of HMF into mice treated with NMDA receptor antagonist MK-801 restored the MK-801-induced deterioration of spatial learning performance in the Morris mater-maze task. Taken together, these results suggest that HMF is a neurotrophic agent for treating patients with memory disorders.

IJMS, Vol. 13, Pages 1805-1831: Aggregating Data for Computational Toxicology Applications: The U.S. Environmental Protection Agency (EPA) Aggregated Computational Toxicology Resource (ACToR) System

Richard S. Judson — Thu, 09 Feb 2012 00:00:00 CET

Computational toxicology combines data from high-throughput test methods, chemical structure analyses and other biological domains (e.g., genes, proteins, cells, tissues) with the goals of predicting and understanding the underlying mechanistic causes of chemical toxicity and for predicting toxicity of new chemicals and products. A key feature of such approaches is their reliance on knowledge extracted from large collections of data and data sets in computable formats. The U.S. Environmental Protection Agency (EPA) has developed a large data resource called ACToR (Aggregated Computational Toxicology Resource) to support these data-intensive efforts. ACToR comprises four main repositories: core ACToR (chemical identifiers and structures, and summary data on hazard, exposure, use, and other domains), ToxRefDB (Toxicity Reference Database, a compilation of detailed in vivo toxicity data from guideline studies), ExpoCastDB (detailed human exposure data from observational studies of selected chemicals), and ToxCastDB (data from high-throughput screening programs, including links to underlying biological information related to genes and pathways). The EPA DSSTox (Distributed Structure-Searchable Toxicity) program provides expert-reviewed chemical structures and associated information for these and other high-interest public inventories. Overall, the ACToR system contains information on about 400,000 chemicals from 1100 different sources. The entire system is built using open source tools and is freely available to download. This review describes the organization of the data repository and provides selected examples of use cases.

IJMS, Vol. 13, Pages 1804: Retraction: Zhong Ye; Darya O. Mishchuk; Natasha S. Stephens and Carolyn M. Slupsky. Dextran Sulfate Sodium Inhibits Alanine Synthesis in Caco-2 Cells. Int. J. Mol. Sci. 2011, 12, 2325-2335.

Shu-Kun Lin — Thu, 09 Feb 2012 00:00:00 CET

It has been brought to our attention by the corresponding author that the results presented this article [1] are in error due to the fact that the media supplement glutaMAX was used in place of L-glutamine for culture of the control cells, while L-glutamine was used for culture of the treated cells. All authors have confirmed that the reported result could not be reproduced using the correct culture conditions. We would like to thank the authors for pointing out this error thereby upholding the ethics of scientific publication. The Editorial Team and Publisher have agreed with the authors that this manuscript should be retracted. We apologize for any inconvenience this may have caused.

Materials, Vol. 5, Pages 258-277: Ceramic Laser Materials

Jasbinder Sanghera — Thu, 09 Feb 2012 00:00:00 CET

Ceramic laser materials have come a long way since the first demonstration of lasing in 1964. Improvements in powder synthesis and ceramic sintering as well as novel ideas have led to notable achievements. These include the first Nd:yttrium aluminum garnet (YAG) ceramic laser in 1995, breaking the 1 KW mark in 2002 and then the remarkable demonstration of more than 100 KW output power from a YAG ceramic laser system in 2009. Additional developments have included highly doped microchip lasers, ultrashort pulse lasers, novel materials such as sesquioxides, fluoride ceramic lasers, selenide ceramic lasers in the 2 to 3 μm region, composite ceramic lasers for better thermal management, and single crystal lasers derived from polycrystalline ceramics. This paper highlights some of these notable achievements.

Pharmaceutics, Vol. 4, Pages 164-178: In Situ Focused Beam Reflectance Measurement (FBRM), Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) and Raman Characterization of the Polymorphic Transformation of Carbamazepine

Yingying Zhao — Thu, 09 Feb 2012 00:00:00 CET

The objective of this work was to study the polymorphic transformation of carbamazepine from Form II to Form III in 1-propanol during seeded isothermal batch crystallization. First, the pure Form II and Form III were obtained and characterized. Then their solubilities and metastable zone limits were measured by in-situ attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and focused beam reflectance measurement (FBRM). A transition temperature at about 34.2 °C was deduced suggesting the enantiotropic nature of this compound over the studied temperature range. To quantify the polymorph ratio during the transformation process, a new in-situ quantitative method was developed to measure the fraction of Form II by Raman spectroscopy. Successful tracking of the nucleation of the stable form and the transformation from Form II to Form III during isothermal crystallization was achieved by Raman spectroscopy and FBRM. The results from these three in-situ techniques, FBRM, FTIR and Raman were consistent with each other. The results showed a strong dependency on the amount of seeds added during isothermal crystallization.

Symmetry, Vol. 4, Pages 39-115: Knots on a Torus: A Model of the Elementary Particles

Jack S. Avrin — Thu, 09 Feb 2012 00:00:00 CET

Two knots; just two rudimentary knots, the unknot and the trefoil. That’s all we need to build a model of the elementary particles of physics, one with fermions and bosons, hadrons and leptons, interactions weak and strong and the attributes of spin, isospin, mass, charge, CPT invariance and more. There are no quarks to provide fractional charge, no gluons to sequester them within nucleons and no “colors” to avoid violating Pauli’s principle. Nor do we require the importation of an enigmatic Higgs boson to confer mass upon the particles of our world. All the requisite attributes emerge simply (and relativistically invariant) as a result of particle conformation and occupation in and of spacetime itself, a spacetime endowed with the imprimature of general relativity. Also emerging are some novel tools for systemizing the particle taxonomy as governed by the gauge group SU(2) and the details of particle degeneracy as well as connections to Hopf algebra, Dirac theory, string theory, topological quantum field theory and dark matter. One exception: it is found necessary to invoke the munificent geometry of the icosahedron in order to provide, as per the group “flavor” SU(3), a scaffold upon which to organize the well-known three generations—no more, no less—of the particle family tree.

Atmosphere, Vol. 3, Pages 132-163: The Impact of Uncertainties in African Biomass Burning Emission Estimates on Modeling Global Air Quality, Long Range Transport and Tropospheric Chemical Lifetimes

Jason E. Williams — Thu, 09 Feb 2012 00:00:00 CET

The chemical composition of the troposphere in the tropics and Southern Hemisphere (SH) is significantly influenced by gaseous emissions released from African biomass burning (BB). Here we investigate how various emission estimates given in bottom-up BB inventories (GFEDv2, GFEDv3, AMMABB) affect simulations of global tropospheric composition using the TM4 chemistry transport model. The application of various model parameterizations for introducing such emissions is also investigated. There are perturbations in near-surface ozone (O3) and carbon monoxide (CO) of ~60–90% in the tropics and ~5–10% in the SH between different inventories. Increasing the update frequency of the temporal distribution to eight days generally results in decreases of between ~5 and 10% in near-surface mixing ratios throughout the tropics, which is larger than the influence of increasing the injection heights at which BB emissions are introduced. There are also associated differences in the long range transport of pollutants throughout the SH, where the composition of the free troposphere in the SH is sensitive to the chosen BB inventory. Analysis of the chemical budget terms reveals that the influence of increasing the tropospheric CO burden due to BB on oxidative capacity of the troposphere is mitigated by the associated increase in NOx emissions (and thus O3) with the variations in the CO/N ratio between inventories being low. For all inventories there is a decrease in the tropospheric chemical lifetime of methane of between 0.4 and 0.8% regardless of the CO emitted from African BB. This has implications for assessing the effect of inter-annual variability in BB on the annual growth rate of methane.

Molecules, Vol. 17, Pages 1686-1697: Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal

Stefano Dall’Acqua — Wed, 08 Feb 2012 00:00:00 CET

The bark of several coniferous species, a waste product of the timber industry, contains significant amounts of natural antioxidants. In our ongoing studies of Nepalese medicinal plants, we examined the bark from Abies spectabilis as the starting material for extracting antioxidant compounds. In vitro antioxidant activity evaluated by means of three antioxidant methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), Briggs-Rauscher oscillating reaction (BR) and Trolox Equivalent Antioxidant Capacity (TEAC) and total phenol contents with the Folin-Ciocalteau reagent; the ferrous iron chelating capacity was also assessed. The methanol extract of A. spectabilis showed significant antioxidant activity and polyphenol contents (IC50 4.13 µg/mL, 0.20 μg/mL eq. resorcinol, 4.22 mM eq. Trolox, 3.9 µg/g eq. gallic Acid in the DPPH, BR, TEAC and Folin-Ciocalteau tests, respectively) and weak Fe2+ chelating capacity. Phytochemical studies were also carried out with 1D- and 2D NMR experiments and DI-ESI-MS, HPLC-DAD and LC-MSn measurements. Oligomeric C-type proanthocyanidins, mainly trimeric gallocatechin derivatives, were the most abundant compounds (16% of extract expressed as procyanindin B1). Gallocatechin oligomers (up to six units) and prodelphynidin-gallocatechin polymers were also identified in the extract. Prodelphynidin B4, cyclograndisolide and trans-docosanil ferulate were also isolated and characterized by NMR and MS spectroscopy.

Molecules, Vol. 17, Pages 1675-1685: Multicomponent Synthesis of 3,6-Dihydro-2H-1,3-thiazine-2-thiones

Art Kruithof — Wed, 08 Feb 2012 00:00:00 CET

Non-fused 3,6-dihydro-2H-1,3-thiazine-2-thiones constitute a so far rather unexplored class of compounds, with the latest report dating back more than two decades. Thiazine-2-thiones contain an endocyclic dithiocarbamate group, which is often found in pesticides, in substrates for radical chemistry and in synthetic intermediates towards thioureas and amidines. We now report the multicomponent reaction (MCR) of in situ-generated 1-azadienes with carbon disulfide. With this reaction, a one-step protocol towards the potentially interesting 3,6-dihydro-2H-1,3-thiazine-2-thiones was established and a small library was synthesized.

Molecules, Vol. 17, Pages 1665-1674: Chiral Flavanones from Amygdalus lycioides Spach: Structural Elucidation and Identification of TNFalpha Inhibitors by Bioactivity-guided Fractionation

Raffaella Gaggeri — Wed, 08 Feb 2012 00:00:00 CET

Phytochemical investigation on the Amygdalus lycioides Spach branchelets resulted in the isolation of four chiral flavanones: (2R,3R)-Taxifolin, (2R,3R)-aromadendrin, (S)-5,7,3',5'-tetrahydroxyflavanone and (S)-naringenin. The flavanones were isolated by semi-preparative HPLC, their structures elucidated based on spectroscopic data and their absolute configuration assigned. As a part of our ethnobotanical-directed search for novel TNFα inhibitors, the bioassay-guided fractionation of the n-hexane-acetone (n-Hex-Ac, 1:1 v/v) Amygdalus lycioides Spach branchelets extract was performed. In this way, (S)-naringenin was identified as the constituent responsible for the TNFα blocking effect, being effective in vitro and in vivo after oral administration. This is the first investigation on bioactive secondary metabolites of Amygdalus lycioides Spach branchelets.

Minerals, Vol. 2, Pages 1-10: Management of Sulfide-Bearing Waste, a Challenge for the Mining Industry

Björn Öhlander — Wed, 08 Feb 2012 00:00:00 CET

Oxidation of iron sulfides in waste rock dumps and tailings deposits may result in formation of acid rock drainage (ARD), which often is a challenging problem at mine sites. Therefore, integrating an ARD management plan into the actual mine operations in the early phases of exploration, continuing through the mine life until final closure might be successful and decrease the environmental impact. A thorough characterization of ore and waste should be performed at an early stage. A detailed knowledge of mineralogical composition, chemical composition and physical properties such as grain size, porosity and hydraulic conductivity of the different waste types is necessary for reliable predictions of ARD formation and efficiency of mitigation measures. Different approaches to prevent and mitigate ARD are discussed. Another key element of successfully planning to prevent ARD and to close a mining operation sustainably is to engage the mine stakeholders (regulators, community and government leaders, non-governmental organization (NGOs) and lenders) in helping develop and implement the ARD management plan.

Religions, Vol. 3, Pages 37-49: Inscribing Authority: Female Title Bearers in Jewish Inscriptions

Carrie Duncan — Wed, 08 Feb 2012 00:00:00 CET

This paper investigates representations of gender in the material culture of the ancient synagogue. The pertinent data are numerous dedicatory and funerary inscriptions linking individual Jews, men and women, with titles seemingly associated with leadership in Late Antique synagogues (ca. 200–600 CE). Bernadette Brooten’s influential 1982 monograph argued against the prevailing tendency to characterize these titles as indications of power, authority, and responsibility when associated with men but as meaningless flattery when applied to women. She suggests that synagogue titles denote power, authority and responsibility on all title bearers equally, both men and women. I question the continued utility of proffering female title-holders as enumerable examples of powerful women rescued from their forgotten place in history. Using theoretical insights developed by historians Elizabeth Clark and Gabrielle Spiegel, this paper will engage a comparative analysis with the work of Riet van Bremen and Saba Mahmood to develop new methods of conceptualizing women’s authority in early Jewish communities. I propose that viewing women’s synagogue titles as culturally constructed representations allows for a fruitful inquiry into how women’s titles were used by male-dominated synagogue communities in their self-articulation and public presentation of Judaism.

Membranes, Vol. 2, Pages 40-69: Thin Hydrogel Films for Optical Biosensor Applications

Anca Mateescu — Wed, 08 Feb 2012 00:00:00 CET

Hydrogel materials consisting of water-swollen polymer networks exhibit a large number of specific properties highly attractive for a variety of optical biosensor applications. This properties profile embraces the aqueous swelling medium as the basis of biocompatibility, non-fouling behavior, and being not cell toxic, while providing high optical quality and transparency. The present review focuses on some of the most interesting aspects of surface-attached hydrogel films as active binding matrices in optical biosensors based on surface plasmon resonance and optical waveguide mode spectroscopy. In particular, the chemical nature, specific properties, and applications of such hydrogel surface architectures for highly sensitive affinity biosensors based on evanescent wave optics are discussed. The specific class of responsive hydrogel systems, which can change their physical state in response to externally applied stimuli, have found large interest as sophisticated materials that provide a complex behavior to hydrogel-based sensing devices.

Micromachines, Vol. 3, Pages 55-61: Surface Plasmon Excitation and Localization by Metal-Coated Axicon Prism

Atsushi Ono — Wed, 08 Feb 2012 00:00:00 CET

Collimated Gaussian beams are efficiently localized at the apex of a metal-coated axicon prism by surface plasmon excitations. We observed the light scattered at the apex and the light reflected by the prism. Intense scattered light was observed with the radial polarization incidence. Further, each incidence of the radial, azimuthal, and linear polarizations provided field distributions of bright and dark intensities in the reflected images according to the surface plasmon excitation. We have demonstrated that surface plasmon waves are excited at the sides of the prism in the Kretschmann configuration and that they converge to its apex.

IJMS, Vol. 13, Pages 1790-1803: Lipid Peroxidative Damage on Cisplatin Exposure and Alterations in Antioxidant Defense System in Rat Kidneys: A Possible Protective Effect of Selenium

Branka I. Ognjanović — Wed, 08 Feb 2012 00:00:00 CET

Cisplatin (Cis-diamminedichloroplatinum II, CP) is an important chemotherapeutic agent, useful in the treatment of several cancers, but with several side effects such as nephrotoxicity. The present study investigated the possible protective effect of selenium (Se) against CP-induced oxidative stress in the rat kidneys. Male Wistar albino rats were injected with a single dose of cisplatin (7 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.), alone or in combination. The obtained results showed that CP increased lipid peroxidation (LPO) and decreased reduced glutathione (GSH) concentrations, suggesting the CP-induced oxidative stress, while Se treatment reversed this change to control values. Acute intoxication of rats with CP was followed by statistically significant decreased activity of antioxidant defense enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and glutathione-S-transferase (GST). Treatment with Se reversed CP-induced alterations of antioxidant defense enzyme activities and significantly prevented the CP-induced kidney damage.

IJMS, Vol. 13, Pages 1778-1789: Cinnamic Acid and Its Derivatives Inhibit Fructose-Mediated Protein Glycation

Sirichai Adisakwattana — Wed, 08 Feb 2012 00:00:00 CET

Cinnamic acid and its derivatives have shown a variety of pharmacologic properties. However, little is known about the antiglycation properties of cinnamic acid and its derivatives. The present study sought to characterize the protein glycation inhibitory activity of cinnamic acid and its derivatives in a bovine serum albumin (BSA)/fructose system. The results demonstrated that cinnamic acid and its derivatives significantly inhibited the formation of advanced glycation end products (AGEs) by approximately 11.96–63.36% at a concentration of 1 mM. The strongest inhibitory activity against the formation of AGEs was shown by cinnamic acid. Furthermore, cinnamic acid and its derivatives reduced the level of fructosamine, the formation of Nε-(carboxymethyl) lysine (CML), and the level of amyloid cross β-structure. Cinnamic acid and its derivatives also prevented oxidative protein damages, including effects on protein carbonyl formation and thiol oxidation of BSA. Our findings may lead to the possibility of using cinnamic acid and its derivatives for preventing AGE-mediated diabetic complications.

IJMS, Vol. 13, Pages 1762-1777: 10-Shogaol, an Antioxidant from Zingiber officinale for Skin Cell Proliferation and Migration Enhancer

Chung-Yi Chen — Wed, 08 Feb 2012 00:00:00 CET

In this work, one of Zingiber officinale components, 10-shogaol, was tested with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, metal chelating ability, and reducing power to show antioxidant activity. 10-Shogaol promoted human normal epidermal keratinocytes and dermal fibroblasts cell growths. 10-Shogaol enhanced growth factor production in transforming growth factor-β (TGF-β), platelet derived growth factor-αβ (PDGF-αβ) and vascular endothelial growth factors (VEGF) of both cells. In the in vitro wound healing assay for 12 or 24 h, with 10-shogaol, the fibroblasts and keratinocytes migrated more rapidly than the vehicle control group. Thus, this study substantiates the target compound, 10-shogaol, as an antioxidant for human skin cell growth and a migration enhancer with potential to be a novel wound repair agent.

Cancers, Vol. 4, Pages 113-129: Medical Treatment of Gastroenteropancreatic Neuroendocrine Tumors

Anja Rinke — Wed, 08 Feb 2012 00:00:00 CET

Treatment of the clinically and prognostically heterogeneous neuroendocrine neoplasms (NEN) should be based on a multidisciplinary approach, including surgical, interventional, medical and nuclear medicine-based therapeutic options. Medical therapies include somatostatin analogues, interferon-a, mTOR inhibitors, multikinase inhibitors and systemic chemotherapy. For the selection of the appropriate medical treatment the hormonal activity, primary tumor localization, tumor grading and growth behaviour as well as the extent of the disease must be considered. Somatostatin analogues are mainly indicated in hormonally active tumors for symptomatic relief, but antiproliferative effects have also been demonstrated, especially in well-differentiated intestinal NET. The efficacy of everolimus and sunitinib in patients with pancreatic neuroendocrine tumors (pNET) has been demonstrated in large placebo-controlled clinical trials. pNETs are also chemosensitive. Streptozocin-based chemotherapeutic regimens are regarded as current standard of care. Temozolomide in combination with capecitabine is an alternative that has shown promising results that need to be confirmed in larger trials. Currently, no comparative studies and no molecular markers are established that predict the response to medical treatment. Therefore the choice of treatment for each pNET patient is based on individual parameters taking into account the patient’s preference, expected side effects and established response criteria such as proliferation rate and tumor load. Platin-based chemotherapy is still the standard treatment for poorly differentiated neuroendocrine carcinomas. Clearly, there is an unmet need for new systemic treatment options in patients with extrapancreatic neuroendocrine tumors.

Cancers, Vol. 4, Pages 100-112: PET-Guided Surgery — High Correlation between Positron Emission Tomography with 11C-5-Hydroxytryptophane (5-HTP) and Surgical Findings in Abdominal Neuroendocrine Tumours

Håkan Örlefors — Wed, 08 Feb 2012 00:00:00 CET

Positron emission tomography (PET) with 11C-labeled 5-hydroxytryptophane (5-HTP) is a sensitive technique to visualize neuroendocrine tumours (NETs), due to high intracellular uptake of amine-precursors like L-dihydroxyphenylalanine (L-DOPA) and 5-HTP. NETs are often small and difficult to localize in spite of overt clinical symptoms due to hormonal excess. In our study, 38 consecutive NET patients underwent 11C-5-HTP-PET and morphological imaging by CT within 12 weeks prior to surgery. Surgical, histopathological and 5-HTP PET findings were correlated. 11C-5-HTP-PET corresponded to the surgical findings in 31 cases, was false negative in six, and true negative in one case resulting in 83.8% sensitivity and 100% specificity. Positive predicted value was 100%. In 11 patients 11C-5-HTP-PET was the only imaging method applied to localize the tumour. Thus, we could demonstrate that functional imaging by 11C-5-HTP-PET in many cases adds vital preoperative diagnostic information and in more than every fourth patient was the only imaging method that will guide the surgeon in finding the NET-lesion. Although the present results demonstrates that 11C-5-HTP may be used as an universal NET tracer, the sensitivity to visualize benign insulinomas and non functioning pancreatic NETs was lower.

Energies, Vol. 5, Pages 270-290: Experimental Investigation on NOx Reduction by Primary Measures in Biomass Combustion: Straw, Peat, Sewage Sludge, Forest Residues and Wood Pellets

Ehsan Houshfar — Wed, 08 Feb 2012 00:00:00 CET

An experimental investigation was carried out to study the NOx formation and reduction by primary measures for five types of biomass (straw, peat, sewage sludge, forest residues/Grot, and wood pellets) and their mixtures. To minimize the NOx level in biomass-fired boilers, combustion experiments were performed in a laboratory scale multifuel fixed grate reactor using staged air combustion. Flue gas was extracted to measure final levels of CO, CO2, CxHy, O2, NO, NO2, N2O, and other species. The fuel gas compositions between the first and second stage were also monitored. The experiments showed good combustion quality with very low concentrations of unburnt species in the flue gas. Under optimum conditions, a NOx reduction of 50–80% was achieved, where the highest reduction represents the case with the highest fuel-N content. The NOx emission levels were very sensitive to the primary excess air ratio and an optimum value for primary excess air ratio was seen at about 0.9. Conversion of fuel nitrogen to NOx showed great dependency on the initial fuel-N content, where the blend with the highest nitrogen content had lowest conversion rate. Between 1–25% of the fuel-N content is converted to NOx depending on the fuel blend and excess air ratio. Sewage sludge is suggested as a favorable fuel to be blended with straw. It resulted in a higher NOx reduction and low fuel-N conversion to NOx. Tops and branches did not show desirable NOx reduction and made the combustion also more unstable. N2O emissions were very low, typically below 5 ppm at 11% O2 in the dry flue gas, except for mixtures with high nitrogen content, where values up to 20 ppm were observed. The presented results are part of a larger study on problematic fuels, also considering ash content and corrosive compounds which have been discussed elsewhere.

Sensors, Vol. 12, Pages 1720-1737: Signal Processing of MEMS Gyroscope Arrays to Improve Accuracy Using a 1st Order Markov for Rate Signal Modeling

Chengyu Jiang — Tue, 07 Feb 2012 00:00:00 CET

This paper presents a signal processing technique to improve angular rate accuracy of the gyroscope by combining the outputs of an array of MEMS gyroscope. A mathematical model for the accuracy improvement was described and a Kalman filter (KF) was designed to obtain optimal rate estimates. Especially, the rate signal was modeled by a first-order Markov process instead of a random walk to improve overall performance. The accuracy of the combined rate signal and affecting factors were analyzed using a steady-state covariance. A system comprising a six-gyroscope array was developed to test the presented KF. Experimental tests proved that the presented model was effective at improving the gyroscope accuracy. The experimental results indicated that six identical gyroscopes with an ARW noise of 6.2 °/√h and a bias drift of 54.14 °/h could be combined into a rate signal with an ARW noise of 1.8 °/√h and a bias drift of 16.3 °/h, while the estimated rate signal by the random walk model has an ARW noise of 2.4 °/√h and a bias drift of 20.6 °/h. It revealed that both models could improve the angular rate accuracy and have a similar performance in static condition. In dynamic condition, the test results showed that the first-order Markov process model could reduce the dynamic errors 20% more than the random walk model.

Sensors, Vol. 12, Pages 1702-1719: Social Network Extraction and Analysis Based on Multimodal Dyadic Interaction

Sergio Escalera — Tue, 07 Feb 2012 00:00:00 CET

Social interactions are a very important component in people’s lives. Social network analysis has become a common technique used to model and quantify the properties of social interactions. In this paper, we propose an integrated framework to explore the characteristics of a social network extracted from multimodal dyadic interactions. For our study, we used a set of videos belonging to New York Times’ Blogging Heads opinion blog. The Social Network is represented as an oriented graph, whose directed links are determined by the Influence Model. The links’ weights are a measure of the “influence” a person has over the other. The states of the Influence Model encode automatically extracted audio/visual features from our videos using state-of-the art algorithms. Our results are reported in terms of accuracy of audio/visual data fusion for speaker segmentation and centrality measures used to characterize the extracted social network.

Sensors, Vol. 12, Pages 1688-1701: A Web Service-Based Framework Model for People-Centric Sensing Applications Applied to Social Networking

David Nunes — Tue, 07 Feb 2012 00:00:00 CET

As the Internet evolved, social networks (such as Facebook) have bloomed and brought together an astonishing number of users. Mashing up mobile phones and sensors with these social environments enables the creation of people-centric sensing systems which have great potential for expanding our current social networking usage. However, such systems also have many associated technical challenges, such as privacy concerns, activity detection mechanisms or intermittent connectivity, as well as limitations due to the heterogeneity of sensor nodes and networks. Considering the openness of the Web 2.0, good technical solutions for these cases consist of frameworks that expose sensing data and functionalities as common Web-Services. This paper presents our RESTful Web Service-based model for people-centric sensing frameworks, which uses sensors and mobile phones to detect users’ activities and locations, sharing this information amongst the user’s friends within a social networking site. We also present some screenshot results of our experimental prototype.

Sensors, Vol. 12, Pages 1657-1687: Noble Metal Nanoparticles for Biosensing Applications

Gonçalo Doria — Tue, 07 Feb 2012 00:00:00 CET

In the last decade the use of nanomaterials has been having a great impact in biosensing. In particular, the unique properties of noble metal nanoparticles have allowed for the development of new biosensing platforms with enhanced capabilities in the specific detection of bioanalytes. Noble metal nanoparticles show unique physicochemical properties (such as ease of functionalization via simple chemistry and high surface-to-volume ratios) that allied with their unique spectral and optical properties have prompted the development of a plethora of biosensing platforms. Additionally, they also provide an additional or enhanced layer of application for commonly used techniques, such as fluorescence, infrared and Raman spectroscopy. Herein we review the use of noble metal nanoparticles for biosensing strategies—from synthesis and functionalization to integration in molecular diagnostics platforms, with special focus on those that have made their way into the diagnostics laboratory.

Sensors, Vol. 12, Pages 1648-1656: Protein Reporter Bioassay Systems for the Phenotypic Screening of Candidate Drugs: A Mouse Platform for Anti-Aging Drug Screening

Takuya Chiba — Tue, 07 Feb 2012 00:00:00 CET

Recent drug discovery efforts have utilized high throughput screening (HTS) of large chemical libraries to identify compounds that modify the activity of discrete molecular targets. The molecular target approach to drug screening is widely used in the pharmaceutical and biotechnology industries, because of the amount of knowledge now available regarding protein structure that has been obtained by computer simulation. The molecular target approach requires that the structure of target molecules, and an understanding of their physiological functions, is known. This approach to drug discovery may, however, limit the identification of novel drugs. As an alternative, the phenotypic- or pathway-screening approach to drug discovery is gaining popularity, particularly in the academic sector. This approach not only provides the opportunity to identify promising drug candidates, but also enables novel information regarding biological pathways to be unveiled. Reporter assays are a powerful tool for the phenotypic screening of compound libraries. Of the various reporter genes that can be used in such assays, those encoding secreted proteins enable the screening of hit molecules in both living cells and animals. Cell- and animal-based screens enable simultaneous evaluation of drug metabolism or toxicity with biological activity. Therefore, drug candidates identified in these screens may have increased biological efficacy and a lower risk of side effects in humans. In this article, we review the reporter bioassay systems available for phenotypic drug discovery.

Sensors, Vol. 12, Pages 1625-1647: E-SAP: Efficient-Strong Authentication Protocol for Healthcare Applications Using Wireless Medical Sensor Networks

Pardeep Kumar — Tue, 07 Feb 2012 00:00:00 CET

A wireless medical sensor network (WMSN) can sense humans’ physiological signs without sacrificing patient comfort and transmit patient vital signs to health professionals’ hand-held devices. The patient physiological data are highly sensitive and WMSNs are extremely vulnerable to many attacks. Therefore, it must be ensured that patients’ medical signs are not exposed to unauthorized users. Consequently, strong user authentication is the main concern for the success and large scale deployment of WMSNs. In this regard, this paper presents an efficient, strong authentication protocol, named E-SAP, for healthcare application using WMSNs. The proposed E-SAP includes: (1) a two-factor (i.e., password and smartcard) professional authentication; (2) mutual authentication between the professional and the medical sensor; (3) symmetric encryption/decryption for providing message confidentiality; (4) establishment of a secure session key at the end of authentication; and (5) professionals can change their password. Further, the proposed protocol requires three message exchanges between the professional, medical sensor node and gateway node, and achieves efficiency (i.e., low computation and communication cost). Through the formal analysis, security analysis and performance analysis, we demonstrate that E-SAP is more secure against many practical attacks, and allows a tradeoff between the security and the performance cost for healthcare application using WMSNs.

Sensors, Vol. 12, Pages 1612-1624: Fast Decision Algorithms in Low-Power Embedded Processors for Quality-of-Service Based Connectivity of Mobile Sensors in Heterogeneous Wireless Sensor Networks

María D. Jaraíz-Simón — Tue, 07 Feb 2012 00:00:00 CET

When a mobile wireless sensor is moving along heterogeneous wireless sensor networks, it can be under the coverage of more than one network many times. In these situations, the Vertical Handoff process can happen, where the mobile sensor decides to change its connection from a network to the best network among the available ones according to their quality of service characteristics. A fitness function is used for the handoff decision, being desirable to minimize it. This is an optimization problem which consists of the adjustment of a set of weights for the quality of service. Solving this problem efficiently is relevant to heterogeneous wireless sensor networks in many advanced applications. Numerous works can be found in the literature dealing with the vertical handoff decision, although they all suffer from the same shortfall: a non-comparable efficiency. Therefore, the aim of this work is twofold: first, to develop a fast decision algorithm that explores the entire space of possible combinations of weights, searching that one that minimizes the fitness function; and second, to design and implement a system on chip architecture based on reconfigurable hardware and embedded processors to achieve several goals necessary for competitive mobile terminals: good performance, low power consumption, low economic cost, and small area integration.

Sensors, Vol. 12, Pages 1594-1611: Gait Analysis Using Floor Markers and Inertial Sensors

Tri Nhut Do — Tue, 07 Feb 2012 00:00:00 CET

In this paper, a gait analysis system which estimates step length and foot angles is proposed. A measurement unit, which consists of a camera and inertial sensors, is installed on a shoe. When the foot touches the floor, markers are recognized by the camera to obtain the current position and attitude. A simple planar marker with 4,096 different codes is used. These markers printed on paper are placed on the floor. When the foot is moving off the floor, the position and attitude are estimated using an inertial navigation algorithm. For accurate estimation, a smoother is proposed, where vision information and inertial sensor data are combined. Through experiments, it is shown that the proposed system can both track foot motion and estimate step length.

IJERPH, Vol. 9, Pages 511-520: Mineral Contamination from Cemetery Soils: Case Study of Zandfontein Cemetery, South Africa

Cornelia Jonker — Tue, 07 Feb 2012 00:00:00 CET

The burial of coffins may pose an environmental and health hazard since the metals that are used in coffin-making may corrode or degrade into harmful toxins. These may leach into the surrounding soils and groundwater. Very little research has been conducted world-wide on the mineral contamination potential of cemeteries, and virtually none in South Africa. The aim of the study is to determine whether burial practices affect the mineral content of soils in cemeteries. This was done by comparing the mineral concentrations of soils within the Zandfontein Cemetery in Tshwane (Gauteng, South Africa) to those off-site as well as those in zones with high burial loads with those zones with fewer burials. Twenty three soil samples were collected from various sites on- and off-site and analyzed for 31 minerals using ICP-AES. It was found that mineral concentrations of soils within the Zandfontein Cemetery were considerably higher than those off-site. Soil samples in multiple burials blocks also have elevated metal concentrations. These excess metals are probably of anthropogenic origin associated with burial practices and could pose an environmental and human health hazard. Strict monitoring of water quality in boreholes in the vicinity of the cemetery is recommended.

Sustainability, Vol. 4, Pages 227-243: Employment Effects of Renewable Energy Expansion on a Regional Level—First Results of a Model-Based Approach for Germany

Philip Ulrich — Tue, 07 Feb 2012 00:00:00 CET

National studies have shown that both gross and net effects of the expansion of energy from renewable sources on employment are positive for Germany. These modeling approaches also revealed that this holds true for both present and future perspectives under certain assumptions on the development of exports, fossil fuel prices and national politics. Yet how are employment effects distributed within Germany? What components contribute to growth impacts on a regional level? To answer these questions (new) methods of regionalization were explored and developed for the example “wind energy onshore” for Germany’s federal states. The main goal was to develop a methodology which is applicable to all renewable energy technologies in future research. For the quantification and projection, it was necessary to distinguish between jobs generated by domestic investments and exports on the one hand, and jobs for operation and maintenance of existing plants on the other hand. Further, direct and indirect employment is analyzed. The results show, that gross employment is particularly high in the northwestern regions of Germany. However, especially the indirect effects are spread out over the whole country. Regions in the south not only profit from the delivery of specific components, but also from other industry and service inputs.

Molbank, Vol. 2012, Pages M749: Ethyl 3-{2-[(3-Methyl-1H-indol-2-yl)carbonyl]hydrazinylidene}butanoate

Thoraya A. Farghaly — Tue, 07 Feb 2012 00:00:00 CET

The title compound, ethyl 3-{2-[(3-methyl-1H-indol-2-yl)carbonyl]hydrazinylidene} butanoate (3), was prepared via reaction of 3-methyl-1H-indole-2-carbohydrazide (1) and ethyl 3-oxobutanoate (2) under reflux. The structure of the synthesized compound was assigned on the basis of elemental analysis, IR, 1H-NMR, mass spectral and X-ray data.

Membranes, Vol. 2, Pages 16-39: Stimuli Responsive Ionogels for Sensing Applications—An Overview

Andrew Kavanagh — Tue, 07 Feb 2012 00:00:00 CET

This overview aims to summarize the existing potential of “Ionogels” as a platform to develop stimuli responsive materials. Ionogels are a class of materials that contain an Ionic Liquid (IL) confined within a polymer matrix. Recently defined as “a solid interconnected network spreading throughout a liquid phase”, the ionogel therefore combines the properties of both its solid and liquid components. ILs are low melting salts that exist as liquids composed entirely of cations and anions at or around 100 °C. Important physical properties of these liquids such as viscosity, density, melting point and conductivity can be altered to suit a purpose by choice of the cation/anion. Here we provide an overview to highlight the literature thus far, detailing the encapsulation of IL and responsive materials within these polymeric structures. Exciting applications in the areas of optical and electrochemical sensing, solid state electrolytes and actuating materials shall be discussed.

Cancers, Vol. 4, Pages 88-99: Transition of Treatment for Patients with Extra-Abdominal Desmoid Tumors: Nagoya University Modality

Yoshihiro Nishida — Tue, 07 Feb 2012 00:00:00 CET

Treatment modalities for desmoid tumors have been changed because of the high recurrence rate, even after wide resection, and some cases experience spontaneous self-regression during clinical course. The treatment modality in our institutions before 2003 was surgical resection with wide surgical margin, however, meloxicam, which is a NSAID and a selective COX-2 inhibitor has been applied consecutively since 2003. We reviewed the previously reported outcomes of surgical and conservative treatment in our institutions. Among 30 patients receiving surgical treatment, 16 (53%) recurred. Younger age ( p < 0.05) was a significant poor factor. According to RECIST for meloxicam treatment, CR was in one, PR in 10, SD in eight, PD in one evaluated at 2011. Older age ( p < 0.01) was significantly associated with good outcome for meloxicam treatment. Results of the previous study indicated that surgical treatment alone could not control desmoid tumors, even with negative surgical margin. Considering the functional impairment resulting from surgery with negative surgical margin, a conservative and effective treatment modality with fewer complications is desired. Conservative treatment with meloxicam is a promising novel modality for patients with extra-abdominal desmoid tumors.

Nutrients, Vol. 4, Pages 78-90: Antiapoptotic and Antiautophagic Effects of Eicosapentaenoic Acid in Cardiac Myoblasts Exposed to Palmitic Acid

Silvia Cetrullo — Tue, 07 Feb 2012 00:00:00 CET

Apoptosis is a programmed cell death that plays a critical role in cell homeostasis. In particular, apoptosis in cardiomyocytes is involved in several cardiovascular diseases including heart failure. Recently autophagy has emerged as an important modulator of programmed cell death pathway. Recent evidence indicates that saturated fatty acids induce cell death through apoptosis and this effect is specific for palmitate. On the other hand, n-3 polyunsaturated fatty acids (PUFAs) have been implicated in the protection against cardiovascular diseases, cardiac ischemic damage and myocardial dysfunction. In the present study we show that n-3 PUFA eicosapentaenoic acid (EPA) treatment to culture medium of H9c2 rat cardiomyoblasts protects cells against palmitate-induced apoptosis, as well as counteracts palmitate-mediated increase of autophagy. Further investigation is required to establish whether the antiautophagic effect of EPA may be involved in its cytoprotective outcome and to explore the underlying biochemical mechanisms through which palmitate and EPA control the fate of cardiac cells.

Entropy, Vol. 14, Pages 177-212: Disentangling Complexity from Randomness and Chaos

Lena C. Zuchowski — Tue, 07 Feb 2012 00:00:00 CET

This study aims to disentangle complexity from randomness and chaos, and to present a definition of complexity that emphasizes its epistemically distinct qualities. I will review existing attempts at defining complexity and argue that these suffer from two major faults: a tendency to neglect the underlying dynamics and to focus exclusively on the phenomenology of complex systems; and linguistic imprecisions in describing these phenomenologies. I will argue that the tendency to discuss phenomenology removed from the underlying dynamics is the main root of the difficulties in distinguishing complex from chaotic or random systems. In my own definition, I will explicitly try to avoid these pitfalls. The theoretical contemplations in this paper will be tested on a sample of five models: the random Kac ring, the chaotic CA30, the regular CA90, the complex CA110 and the complex Bak-Sneppen model. Although these modelling studies are restricted in scope and can only be seen as preliminary, they still constitute on of the first attempts to investigate complex systems comparatively.

Biomolecules, Vol. 2, Pages 46-75: Factor H: A Complement Regulator in Health and Disease, and a Mediator of Cellular Interactions

Anne Kopp — Tue, 07 Feb 2012 00:00:00 CET

Complement is an essential part of innate immunity as it participates in host defense against infections, disposal of cellular debris and apoptotic cells, inflammatory processes and modulation of adaptive immune responses. Several soluble and membrane-bound regulators protect the host from the potentially deleterious effects of uncontrolled and misdirected complement activation. Factor H is a major soluble regulator of the alternative complement pathway, but it can also bind to host cells and tissues, protecting them from complement attack. Interactions of factor H with various endogenous ligands, such as pentraxins, extracellular matrix proteins and DNA are important in limiting local complement-mediated inflammation. Impaired regulatory as well as ligand and cell recognition functions of factor H, caused by mutations or autoantibodies, are associated with the kidney diseases: atypical hemolytic uremic syndrome and dense deposit disease and the eye disorder: age-related macular degeneration. In addition, factor H binds to receptors on host cells and is involved in adhesion, phagocytosis and modulation of cell activation. In this review we discuss current concepts on the physiological and pathophysiological roles of factor H in light of new data and recent developments in our understanding of the versatile roles of factor H as an inhibitor of complement activation and inflammation, as well as a mediator of cellular interactions. A detailed knowledge of the functions of factor H in health and disease is expected to unravel novel therapeutic intervention possibilities and to facilitate the development or improvement of therapies.

Energies, Vol. 5, Pages 258-269: Field Surveys of Non-Residential Solar Water Heating Systems in Taiwan

Wei-Min Lin — Tue, 07 Feb 2012 00:00:00 CET

To develop indigenous alternative and renewable energy resources, long-term subsidy programs (1986–1991 and 2000–present) for solar water heaters have been enforced in Taiwan. By the end of 2010, the total installed area of solar collectors had exceeded 2 million square meters. However, over 98% of solar water heaters were used in residential systems for hot water production, with the areas of installed solar collector being less than 10 square meters. There were only 98 systems with area of solar collectors installed exceeding 100 square meters put into operation from 2001 to 2010. These systems were mainly installed for water heating in dormitories, swimming pools, restaurants, and manufacturing plants. In the present study, a comprehensive survey of these large-scale solar water heaters was conducted. The objectives of the survey were to assess the system performance and to collect feedback from individual users. It is found that lack of experience in system design and maintenance are the key factors affecting reliable operation of a system. Hourly, daily and long-term field measurements of a dormitory system were also examined to evaluate its thermal efficiencies. Results indicated that thermal efficiency of the system is associated with the daily solar radiation. Hot water use pattern and operation of auxiliary heater should be taken into account in system design.

Viruses, Vol. 4, Pages 276-279: A Plea for Caution: Huge Risks Associated with Lab-bred Flu

Viktor Müller — Tue, 07 Feb 2012 00:00:00 CET

I wish to express concern about the maintenance of laboratory strains of H5N1 influenza viruses that might be adapted for transmission among humans.

IJMS, Vol. 13, Pages 1747-1761: Mitochondrial Protection and Anti-aging Activity of Astragalus Polysaccharides and Their Potential Mechanism

Xing-Tai Li — Tue, 07 Feb 2012 00:00:00 CET

The current study was performed to investigate mitochondrial protection and anti-aging activity of Astragalus polysaccharides (APS) and the potential underlying mechanism. Lipid peroxidation of liver and brain mitochondria was induced by Fe2+–Vit C in vitro. Thiobarbituric acid (TBA) colorimetry was used to measure the content of thiobarbituric acid reactive substances (TBARS). Mouse liver mitochondrial permeability transition (PT) was induced by calcium overload in vitro and spectrophotometry was used to measure it. The scavenging activities of APS on superoxide anion (O2•－) and hydroxyl radical (•OH), which were produced by reduced nicotinamide adenine dinucleotide (NADH)—N-Methylphenazonium methyl sulfate (PMS) and hydrogen peroxide (H2O2)–Fe2+ system respectively, were measured by 4-nitrobluetetrazolium chloride (NBT) reduction and Fenton reaction colorimetry respectively. The Na2S2O3 titration method was used to measure the scavenging activities of APS on H2O2. APS could inhibit TBARS production, protect mitochondria from PT, and scavenge O2•－, •OH and H2O2 significantly in a concentration-dependent manner respectively. The back of the neck of mice was injected subcutaneously with D-galactose to induce aging at a dose of 100 mg/kg/d for seven weeks. Moreover, the activities of catalase (CAT), surperoxide dismutase (SOD) and glutathione peroxidase (GPx) and anti-hydroxyl radical which were assayed by using commercial monitoring kits were increased significantly in vivo by APS. According to this research, APS protects mitochondria by scavenging reactive oxygen species (ROS), inhibiting mitochondrial PT and increasing the activities of antioxidases. Therefore, APS has the effect of promoting health.

IJMS, Vol. 13, Pages 1733-1746: Characterization of Catalase from Psychrotolerant Psychrobacter piscatorii T-3 Exhibiting High Catalase Activity

Hideyuki Kimoto — Tue, 07 Feb 2012 00:00:00 CET

A psychrotolerant bacterium, strain T-3 (identified as Psychrobacter piscatorii), that exhibited an extraordinarily high catalase activity was isolated from the drain pool of a plant that uses H2O2 as a bleaching agent. Its cell extract exhibited a catalase activity (19,700 U·mg protein−1) that was higher than that of Micrococcus luteus used for industrial catalase production. Catalase was approximately 10% of the total proteins in the cell extract of the strain. The catalase (PktA) was purified homogeneously by only two purification steps, anion exchange and hydrophobic chromatographies. The purified catalase exhibited higher catalytic efficiency and higher sensitivity of activity at high temperatures than M. luteus catalase. The deduced amino acid sequence showed the highest homology with catalase of Psycrobacter cryohalolentis, a psychrotolelant bacterium obtained from Siberian permafrost. These findings suggest that the characteristics of the PktA molecule reflected the taxonomic relationship of the isolate as well as the environmental conditions (low temperatures and high concentrations of H2O2) under which the bacterium survives. Strain T-3 efficiently produces a catalase (PktA) at a higher rate than Exiguobacterium oxidotolerans, which produces a very strong activity of catalase (EktA) at a moderate rate, in order to adapt to high concentration of H2O2.

IJMS, Vol. 13, Pages 1720-1732: The Effect of C-Terminal Helix on the Stability of FF Domain Studied by Molecular Dynamics Simulation

Liling Zhao — Tue, 07 Feb 2012 00:00:00 CET

To investigate the effect of C-terminal helix on the stability of the FF domain, we studied the native domain FF3-71 from human HYPA/FBP11 and the truncated version FF3-60 with C-terminal helix being deleted by molecular dynamics simulations with GROMACS package and GROMOS 43A1 force field. The results indicated that the structures of truncated version FF3-60 were evident different from those of native partner FF3-71. Compared with FF3-71, the FF3-60 lost some native contacts and exhibited some similar structural characters to those of intermediate state. The C-terminal helix played a major role in stabilizing the FF3-71 domain. To a certain degree, the FF domain had a tendency to form an intermediate state without the C-terminal helix. In our knowledge, this was the first study to examine the role of C-terminal helix of FF domain in detail by molecular dynamics simulations, which was useful to understand the three-state folding mechanism of the small FF domain.

Applied Sciences, Vol. 2, Pages 100-113: The Logic-Based Supervisor Control for Sun-Tracking System of 1 MW HCPV Demo Plant: Study Case

Hong-Yih Yeh — Tue, 07 Feb 2012 00:00:00 CET

This paper presents a logic-based supervisor controller designed for trackers for a 1MW HCPV demo plant in Taiwan. A sun position sensor on the tracker is used to detect the sun position, as the sensor is sensitive to the intensity of sun light. The signal output of the sensor is partially affected by the cloud, which has a hard control position with the traditional PID control. Therefore we have used logic-based supervisor (LBS) control which permits switching the PID control to sun trajectory under sunny or cloudy conditions. To verify the stability of the proposed control, an experiment was performed and the results show that the proposed control can efficiently achieve stabilization of the trackers of the 1MW HCPV demo plant.

Molecules, Vol. 17, Pages 1652-1664: Palynological Origin, Phenolic Content, and Antioxidant Properties of Honeybee-Collected Pollen from Bahia, Brazil

Kristerson R. L. Freire — Tue, 07 Feb 2012 00:00:00 CET

The aim of this study was to determine the palynological origin, phenolic and flavonoid content, and antioxidant properties of twenty-five samples of bee pollen harvested during a nine-month period (February–November) from the Canavieiras municipality (northeastern Brazil). Of the 25 samples analyzed, only two (February 01 and 02) were heterofloral. The predominant pollens in the samples analyzed during that month were: Cecropia, Eucalyptus, Elaeis, Mimosa pudica, Eupatorium, and Scoparia. Ethyl acetate fractions were analyzed by HPLC-DAD. The flavonoids isoquercetin, myricetin, tricetin, quercetin, luteolin, selagin, kaempferol, and isorhamnetin were detected. The flavonoid present in all 22 samples was isolated and identified as isorhamnetin 3-O-b-neohesperidoside. The total phenolic contents determined using the Folin-Ciocalteu reagent ranged from 41.5 to 213.2 mg GAE/g. Antioxidant activities based on the 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2-azinobis 3-ethylbenzothiozoline-6-sulfonic acid (ABTS), and Fe2+ ion chelating activity assays were observed for all extracts, and correlated with the total phenolic content.

Molecules, Vol. 17, Pages 1635-1651: Prebiotic Activity of Polysaccharides Extracted from Gigantochloa Levis (Buluh beting) Shoots

Aida Firdaus Muhammad Nurul Azmi — Tue, 07 Feb 2012 00:00:00 CET

Bamboo shoot crude polysaccharides (BSCP) extracted from the shoots of Gigantochloa levis gave about 3.27 ± 0.18% on dry basis and a very minute percentage of protein (0.02 ± 0.01%). The molecular weight of BSCP estimated by gel chromatography was found to be around 7.49 × 103 Da, while the molecular weights of purified fractions (F1 to F5) were around 1550.96, 1471.63, 1685.78, 1691.61 and 1551.67 Da, respectively. The FTIR spectrum of BSCP revealed the possibility that the extract contains β-glucan, which can be considered a valuable compound for the medical and food industries. These relate to the resistance of BSCP towards artificial human gastric juice which is more than 99%. Prebiotic activity tested using BSCP as a carbon source showed significant increase in the growth of B. animalis ATCC 1053, B. longum BB 536 and L. acidophilus ATCC 4356 as compared to the use of FOS. Survivality of S. choleraesuis JCM 6977 was found to be slower in both BSCP and FOS. Study conducted reflects a good sign for the BSCP to be exploited as a promising prebiotic.

Viruses, Vol. 4, Pages 258-275: Filovirus Entry: A Novelty in the Viral Fusion World

Catherine L. Hunt — Tue, 07 Feb 2012 00:00:00 CET

Ebolavirus (EBOV) and Marburgvirus (MARV) that compose the filovirus family of negative strand RNA viruses infect a broad range of mammalian cells. Recent studies indicate that cellular entry of this family of viruses requires a series of cellular protein interactions and molecular mechanisms, some of which are unique to filoviruses and others are commonly used by all viral glycoproteins. Details of this entry pathway are highlighted here. Virus entry into cells is initiated by the interaction of the viral glycoprotein1 subunit (GP1) with both adherence factors and one or more receptors on the surface of host cells. On epithelial cells, we recently demonstrated that TIM-1 serves as a receptor for this family of viruses, but the cell surface receptors in other cell types remain unidentified. Upon receptor binding, the virus is internalized into endosomes primarily via macropinocytosis, but perhaps by other mechanisms as well. Within the acidified endosome, the heavily glycosylated GP1 is cleaved to a smaller form by the low pH-dependent cellular proteases Cathepsin L and B, exposing residues in the receptor binding site (RBS). Details of the molecular events following cathepsin-dependent trimming of GP1 are currently incomplete; however, the processed GP1 specifically interacts with endosomal/lysosomal membranes that contain the Niemann Pick C1 (NPC1) protein and expression of NPC1 is required for productive infection, suggesting that GP/NPC1 interactions may be an important late step in the entry process. Additional events such as further GP1 processing and/or reducing events may also be required to generate a fusion-ready form of the glycoprotein. Once this has been achieved, sequences in the filovirus GP2 subunit mediate viral/cellular membrane fusion via mechanisms similar to those previously described for other enveloped viruses. This multi-step entry pathway highlights the complex and highly orchestrated path of internalization and fusion that appears unique for filoviruses.

Viruses, Vol. 4, Pages 236-257: Emerging Viruses in the Felidae: Shifting Paradigms

Stephen J. O’Brien — Tue, 07 Feb 2012 00:00:00 CET

The domestic cat is afflicted with multiple viruses that serve as powerful models for human disease including cancers, SARS and HIV/AIDS. Cat viruses that cause these diseases have been studied for decades revealing detailed insight concerning transmission, virulence, origins and pathogenesis. Here we review recent genetic advances that have questioned traditional wisdom regarding the origins of virulent Feline infectious peritonitis (FIP) diseases, the pathogenic potential of Feline Immunodeficiency Virus (FIV) in wild non-domestic Felidae species, and the restriction of Feline Leukemia Virus (FeLV) mediated immune impairment to domestic cats rather than other Felidae species. The most recent interpretations indicate important new evolutionary conclusions implicating these deadly infectious agents in domestic and non-domestic felids.

Molecules, Vol. 17, Pages 1617-1634: Enantioselective Synthesis of 2,2-Disubstituted Terminal Epoxides via Catalytic Asymmetric Corey-Chaykovsky Epoxidation of Ketones

Toshihiko Sone — Tue, 07 Feb 2012 00:00:00 CET

Catalytic asymmetric Corey-Chaykovsky epoxidation of various ketones with dimethyloxosulfonium methylide using a heterobimetallic La-Li3-BINOL complex (LLB) is described. The reaction proceeded smoothly at room temperature in the presence of achiral phosphine oxide additives, and 2,2-disubstituted terminal epoxides were obtained in high enantioselectivity (97%–91% ee) and yield ( > 99%–88%) from a broad range of methyl ketones with 1–5 mol% catalyst loading. Enantioselectivity was strongly dependent on the steric hindrance, and other ketones, such as ethyl ketones and propyl ketones resulted in slightly lower enantioselectivity (88%–67% ee).

Molecules, Vol. 17, Pages 1602-1616: Phloxine B as a Probe for Entrapment in Microcrystalline Cellulose

Paulo Duarte — Tue, 07 Feb 2012 00:00:00 CET

The photophysical behaviour of phloxine B adsorbed onto microcrystalline cellulose was evaluated by reflectance spectroscopy and laser induced time-resolved luminescence in the picosecond-nanosecond and microsecond-millisecond ranges. Analysis of the absorption spectral changes with concentration points to a small tendency of the dye to aggregate in the range of concentrations under study. Prompt fluorescence, phosphorescence and delayed fluorescence spectral decays were measured at room temperature and 77 K, without the need of sample degassing because cellulose protects triplet states from oxygen quenching. In all cases, spectral changes with time and lifetime distribution analysis were consistent with the dye coexisting in two different environments: dyes tightly entrapped between polymer chains in crystalline regions of cellulose showed longer fluorescence and phosphorescence lifetimes and more energetic triplet states, while dyes adsorbed in more amorphous regions of the support showed shorter lifetimes and less energetic triplet states. This behaviour is discussed in terms of the different dye-support interactions in both kinds of adsorption sites.

Molecules, Vol. 17, Pages 1571-1601: Anthocyanins and Their Variation in Red Wines I. Monomeric Anthocyanins and Their Color Expression

Fei He — Tue, 07 Feb 2012 00:00:00 CET

Originating in the grapes, monomeric anthocyanins in young red wines contribute the majority of color and the supposed beneficial health effects related to their consumption, and as such they are recognized as one of the most important groups of phenolic metabolites in red wines. In recent years, our increasing knowledge of the chemical complexity of the monomeric anthocyanins, their stability, together with the phenomena such as self-association and copigmentation that can stabilize and enhance their color has helped to explain their color representation in red wine making and aging. A series of new enological practices were developed to improve the anthocyanin extraction, as well as their color expression and maintenance. This paper summarizes the most recent advances in the studies of the monomeric anthocyanins in red wines, emphasizing their origin, occurrence, color enhancing effects, their degradation and the effect of various enological practices on them.

Molecules, Vol. 17, Pages 1548-1570: Benzoxetes and Benzothietes ¾ Heterocyclic Analogues of Benzocyclobutene

Herbert Meier — Tue, 07 Feb 2012 00:00:00 CET

Benzo-condensed four-ring heterocycles, such as benzoxetes 1 and benzothietes 3 represent multi-purpose starting compounds for the preparation of various higher heterocyclic ring systems. The thermal or photochemical valence isomerizations between the benzenoid forms 1,3 and the higher reactive o-quinoid structures 2,4 provide the basis for the synthetic applications. On the other hand, this valence isomerization impedes in particular the generation and storage of 1 because the thermal equilibrium 1 2 is completely on the side of 2. Thus, the number of erroneous or questionable benzoxete structures published to date is surprisingly high. On the contrary, the thermal equilibrium 3 4 is on the side of the benzothietes 3, which makes them easily accessible, especially by different flash vacuum pyrolysis techniques. The present article gives a survey of the preparations of 1 and 2, and tries to stimulate their use in synthetic projects. Naphtho-condensed and higher condensed compounds and compounds with an exocyclic C=O or S=O double bond (lactones, thiolactones, sulfoxides and sulfones) are not covered in this article.

Molecules, Vol. 17, Pages 1535-1547: Carotenoids of Lettuce (Lactuca sativa L.) Grown on Soil Enriched with Spent Coffee Grounds

Rebeca Cruz — Tue, 07 Feb 2012 00:00:00 CET

The impact of spent coffee grounds on carotenoid and chlorophyll content in lettuce (Lactuca sativa L. var. capitata) was evaluated. A greenhouse pot experiment was conducted with spent coffee amounts ranging from 0% to 20% (v/v). All evaluated pigments increased proportionally to spent coffee amounts. Lutein and β-carotene levels increased up to 90% and 72%, respectively, while chlorophylls increased up to 61%. Biomass was also improved in the presence of 2.5% to 10% spent coffee, decreasing for higher amounts. Nevertheless, all plants were characterized by lower organic nitrogen content than the control ones, inversely to the spent coffee amounts, pointing to possible induced stress. Collected data suggests that plants nutritional features, with regards to these bioactive compounds, can be improved by the presence of low amounts of spent coffee grounds (up to 10%). This observation is particularly important because soil amendment with spent coffee grounds is becoming increasingly common within domestic agriculture. Still, further studies on the detailed influence of spent coffee bioactive compounds are mandatory, particularly regarding caffeine.

Molecules, Vol. 17, Pages 1520-1534: Developing Fluorescent Hyaluronan Analogs for Hyaluronan Studies

Wei Wang — Tue, 07 Feb 2012 00:00:00 CET

Two kinds of fluorescent hyaluronan (HA) analogs, one serving as normal imaging agent and the other used as a biosensitive contrast agent, were developed for the investigation of HA uptake and degradation. Our approach of developing HA imaging agents depends on labeling HA with varying molar percentages of a near-infrared (NIR) dye. At low labeling ratios, the hyaluronan uptake can be directly imaged while at high labeling ratios, the fluorescent signal is quenched and signal generation occurs only after degradation. It is found that the conjugate containing 1%–2% NIR dye can be used as a normal optical imaging agent, while bioactivable imaging agents are formed at 6% to 17% dye loading. It was determined that the conjugation of dye to HA with different loading percentages does not impact HA biodegradation by hyaluronidase (Hyal). The feasibility of using these two NIR fluorescent hyaluronan analogs for HA investigation was evaluated in vivo with optical imaging. The data demonstrates that the 1% dye loaded fluorescent HA can be used to monitor the behavior of HA and its fragments, whereas bioactivatable HA imaging agent (17% dye in HA) is more suitable for detecting HA fragments.

Molecules, Vol. 17, Pages 1483-1519: Anthocyanins and Their Variation in Red Wines II. Anthocyanin Derived Pigments and Their Color Evolution

Fei He — Tue, 07 Feb 2012 00:00:00 CET

Originating in the grapes, anthocyanins and their derivatives are the crucial pigments responsible for the red wine color. During wine maturation and aging, the concentration of monomeric anthocyanins declines constantly, while numerous more complex and stable anthocyanin derived pigments are formed, mainly including pyranoanthocyanins, polymeric anthocyanins produced from condensation between anthocyanin and/or flavan-3-ols directly or mediated by aldehydes. Correspondingly, their structural modifications result in a characteristic variation of color, from purple-red color in young red wines to brick-red hue of the aged. Because of the extreme complexity of chemical compounds involved, many investigations have been made using model solutions of know composition rather than wine. Thus, there is a large amount of research still required to obtain an overall perspective of the anthocyanin composition and its change with time in red wines. Future findings may well greatly revise our current interpretation of the color in red wines. This paper summarizes the most recent advances in the studies of the anthocyanins derived pigments in red wines, as well as their color evolution.

Energies, Vol. 5, Pages 243-257: Influence of n-Hexane on in Situ Transesterification of Marine Macroalgae

Angel Sánchez — Mon, 06 Feb 2012 00:00:00 CET

The purpose of this work is to investigate the influence of n-hexane addition on in situ transesterification of a solid raw material for biodiesel production. Extraction and reaction of macroalgae oil has been performed simultaneously in a batch reactor adding n-hexane with the reactants. In order to analyze the influence of n-hexane on the transesterification, the reaction was also carried out with sunflower oil. The results show that the presence of n-hexane does not have an important effect on the transesterification. It was also observed that this method requires large quantities of methanol to carry out the reaction. The best reaction conditions for in situ transesteriﬁcation of marine macroalgae were 300:1 methanol-to-oil molar ratio, 1% catalyst concentration, 60 °C reaction temperature and 11 h reaction time, resulting in a methyl esters yield of 17.1%. Thus, biodiesel production from macroalgae by transesterification in situ could be feasible, using hexane for the extraction and eliminating the previous extraction. This integrated method is thus effective and technically attractive.

Plants, Vol. 1, Pages 1-5: Plants: An International Scientific Open Access Journal to Publish All Facets of Plants, Their Functions and Interactions with the Environment and Other Living Organisms

W.G. Dilantha Fernando — Mon, 06 Feb 2012 00:00:00 CET

Plants are one of the two major groups of living organisms that are an essential entity to the function of the biosphere. Plants can be found in all known parts of the earth, in all shapes and sizes. They include the green algae, mosses, ferns, vines, grasses, bushes, herbs, flowering plants and trees. Although some plants are parasitic, most produce their own food through photosynthesis. Most plants initiate from a seed. The importance of plants in the food chain dates back to ancient times. The first humans gathered wild plants for food. As settlements developed, food crops were cultivated, leading to selection of high-yielding cultivated varieties to feed the growing populations. Unlike plants, humans and other animals are unable to manufacture their own food. Therefore, they are dependent, directly or indirectly, on plants. Plants are found in natural ecosystems such as rain forests, and also in agricultural areas and urbanized settings. They are an essential part of our daily lives providing food, clean air, and important ecosystem functions. The study of plants and their function could be considered the most complex of interactions. From the time a seed germinates, it goes through a myriad of physiological processes that can be closely studied using modern tools and molecular biological methods. An open access journal such as Plants will give millions of readers access to that information around the world.

MDPI Publishing

Diversity, Vol. 4, Pages 59-73: Mosses Like It Rough—Growth Form Specific Responses of Mosses, Herbaceous and Woody Plants to Micro-Relief Heterogeneity

Sensors, Vol. 12, Pages 2005-2017: A Method Based on Multi-Sensor Data Fusion for Fault Detection of Planetary Gearboxes

Sensors, Vol. 12, Pages 1990-2004: Robust Kernel-Based Tracking with Multiple Subtemplates in Vision Guidance System

IJERPH, Vol. 9, Pages 521-530: Age, Gender and Suicidal Ideation Following Voluntary HIV Counseling and Testing

Sensors, Vol. 12, Pages 1967-1989: Secure Cooperation of Autonomous Mobile Sensors Using an Underwater Acoustic Network

Sensors, Vol. 12, Pages 1950-1966: Electrical Model of a Carbon-Polymer Composite (CPC) Collision Detector

Polymers, Vol. 4, Pages 486-500: Enzymatic Synthesis and Crosslinking of Novel High Molecular Weight Polyepoxyricinoleate

Sensors, Vol. 12, Pages 1930-1949: Sunlight Intensity Based Global Positioning System for Near-Surface Underwater Sensors

Polymers, Vol. 4, Pages 463-485: Multiscale Modeling for Host-Guest Chemistry of Dendrimers in Solution

Sensors, Vol. 12, Pages 1919-1929: The Effect of Direction on Cursor Moving Kinematics

Sensors, Vol. 12, Pages 1898-1918: Fiber Bragg Grating Sensors for Harsh Environments

Sensors, Vol. 12, Pages 1885-1897: Gen-2 Hand-Held Optical Imager towards Cancer Imaging: Reflectance and Transillumination Phantom Studies

Sensors, Vol. 12, Pages 1863-1884: Parametric Dense Stereovision Implementation on a System-on Chip (SoC)

Marine Drugs, Vol. 10, Pages 349-357: Two New Tryptamine Derivatives, Leptoclinidamide and (-)-Leptoclinidamine B, from an Indonesian Ascidian Leptoclinides dubius

IJMS, Vol. 13, Pages 1933-1950: Glucose-Modulated Mitochondria Adaptation in Tumor Cells: A Focus on ATP Synthase and Inhibitor Factor 1

IJMS, Vol. 13, Pages 1919-1932: Presence of CP4-EPSPS Component in Roundup Ready Soybean-Derived Food Products

IJMS, Vol. 13, Pages 1886-1918: The mTOR Signalling Pathway in Human Cancer

Molecules, Vol. 17, Pages 1809-1818: Pressurized Hot Ethanol Extraction of Carotenoids from Carrot By-Products

Molecules, Vol. 17, Pages 1797-1808: Phenolic Compounds and Antioxidant Activities of Liriope muscari

Molecules, Vol. 17, Pages 1795-1796: Molecules Best Paper Award 2012

Molecules, Vol. 17, Pages 1764-1794: Applications of Supercritical Fluid Extraction (SFE) of Palm Oil and Oil from Natural Sources

Polymers, Vol. 4, Pages 448-462: In Situ and Ex Situ Syntheses of Magnetic Liquid Crystalline Materials: A Comparison

IJMS, Vol. 13, Pages 1858-1885: Yeast Mitochondrial Interactosome Model: Metabolon Membrane Proteins Complex Involved in the Channeling of ADP/ATP

Entropy, Vol. 14, Pages 233-251: Scientific Élan Vital: Entropy Deficit or Inhomogeneity as a Unified Concept of Driving Forces of Life in Hierarchical Biosphere Driven by Photosynthesis

Molecules, Vol. 17, Pages 1751-1763: Design, Synthesis and Preliminary Pharmacological Evaluation of New Non-Steroidal Anti-Inflammatory Agents Having a 4-(Methylsulfonyl) Aniline Pharmacophore

Entropy, Vol. 14, Pages 213-232: Quantitative Comparison of Conformational Ensembles

Insects, Vol. 3, Pages 161-199: Insect Sex Determination Manipulated by Their Endosymbionts: Incidences, Mechanisms and Implications

Insects, Vol. 3, Pages 141-160: Phylogenetic Analysis of Fusarium solani Associated with the Asian Longhorned Beetle, Anoplophora glabripennis

Insects, Vol. 3, Pages 133-140: Near-Optimal Foraging in the Pacific Cicada Killer Sphecius convallis Patton (Hymenoptera: Crabronidae)

Genes, Vol. 3, Pages 115-137: Genomic Distribution and Divergence of Levansucrase-Coding Genes in Pseudomonas syringae

Agronomy, Vol. 2, Pages 1-13: Wheat Nitrogen Fertilisation Effects on the Performance of the Cereal Aphid Metopolophium dirhodum

Sustainability, Vol. 4, Pages 244-256: Bioenergy Villages and Regions in Germany: An Interview Study with Initiators of Communal Bioenergy Projects on the Success Factors for Restructuring the Energy Supply of the Community

Remote Sensing, Vol. 4, Pages 456-483: How Robust Are Burn Severity Indices When Applied in a New Region? Evaluation of Alternate Field-Based and Remote-Sensing Methods

Sensors, Vol. 12, Pages 1846-1862: Extraction and Analysis of Impervious Surfaces Based on a Spectral Un-Mixing Method Using Pearl River Delta of China Landsat TM/ETM+ Imagery from 1998 to 2008

Sensors, Vol. 12, Pages 1827-1845: AURP: An AUV-Aided Underwater Routing Protocol for Underwater Acoustic Sensor Networks

Sensors, Vol. 12, Pages 1816-1826: Evaluation of Electrical and Optical Plethysmography Sensors for Noninvasive Monitoring of Hemoglobin Concentration

Sensors, Vol. 12, Pages 1800-1815: A Platform for Combined DNA and Protein Microarrays Based on Total Internal Reflection Fluorescence

Sensors, Vol. 12, Pages 1771-1799: Vector Disparity Sensor with Vergence Control for Active Vision Systems

Sensors, Vol. 12, Pages 1758-1770: Comparison between Conduction and Convection Effects on Self-Heating in Doped Microcantilevers

Sensors, Vol. 12, Pages 1738-1757: Finger Vein Recognition Based on a Personalized Best Bit Map

Molecules, Vol. 17, Pages 1744-1750: The Incubation of 13a,17-Dihydroxystemodane with Cephalosporium aphidicola

Molecules, Vol. 17, Pages 1716-1743: Chemisorption and Reactions of Small Molecules on Small Gold Particles

Molecules, Vol. 17, Pages 1698-1715: Ergosterol-Induced Sesquiterpenoid Synthesis in Tobacco Cells

Polymers, Vol. 4, Pages 408-447: Polysilane Dendrimers

IJMS, Vol. 13, Pages 1846-1857: The -308G/A of Tumor Necrosis Factor (TNF)-α and 825C/T of Guanidine Nucleotide Binding Protein 3 (GNB3) are Associated with the Onset of Acute Myocardial Infarction and Obesity in Taiwan

IJMS, Vol. 13, Pages 1832-1845: Isolation and Characterization of Activators of ERK/MAPK from Citrus Plants

IJMS, Vol. 13, Pages 1805-1831: Aggregating Data for Computational Toxicology Applications: The U.S. Environmental Protection Agency (EPA) Aggregated Computational Toxicology Resource (ACToR) System

IJMS, Vol. 13, Pages 1804: Retraction: Zhong Ye; Darya O. Mishchuk; Natasha S. Stephens and Carolyn M. Slupsky. Dextran Sulfate Sodium Inhibits Alanine Synthesis in Caco-2 Cells. Int. J. Mol. Sci. 2011, 12, 2325-2335.

Materials, Vol. 5, Pages 258-277: Ceramic Laser Materials

Pharmaceutics, Vol. 4, Pages 164-178: In Situ Focused Beam Reflectance Measurement (FBRM), Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) and Raman Characterization of the Polymorphic Transformation of Carbamazepine

Symmetry, Vol. 4, Pages 39-115: Knots on a Torus: A Model of the Elementary Particles

Atmosphere, Vol. 3, Pages 132-163: The Impact of Uncertainties in African Biomass Burning Emission Estimates on Modeling Global Air Quality, Long Range Transport and Tropospheric Chemical Lifetimes

Molecules, Vol. 17, Pages 1686-1697: Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal

Molecules, Vol. 17, Pages 1675-1685: Multicomponent Synthesis of 3,6-Dihydro-2H-1,3-thiazine-2-thiones

Molecules, Vol. 17, Pages 1665-1674: Chiral Flavanones from Amygdalus lycioides Spach: Structural Elucidation and Identification of TNFalpha Inhibitors by Bioactivity-guided Fractionation

Minerals, Vol. 2, Pages 1-10: Management of Sulfide-Bearing Waste, a Challenge for the Mining Industry

Religions, Vol. 3, Pages 37-49: Inscribing Authority: Female Title Bearers in Jewish Inscriptions

Membranes, Vol. 2, Pages 40-69: Thin Hydrogel Films for Optical Biosensor Applications

Micromachines, Vol. 3, Pages 55-61: Surface Plasmon Excitation and Localization by Metal-Coated Axicon Prism

IJMS, Vol. 13, Pages 1790-1803: Lipid Peroxidative Damage on Cisplatin Exposure and Alterations in Antioxidant Defense System in Rat Kidneys: A Possible Protective Effect of Selenium

IJMS, Vol. 13, Pages 1778-1789: Cinnamic Acid and Its Derivatives Inhibit Fructose-Mediated Protein Glycation

IJMS, Vol. 13, Pages 1762-1777: 10-Shogaol, an Antioxidant from Zingiber officinale for Skin Cell Proliferation and Migration Enhancer

Cancers, Vol. 4, Pages 113-129: Medical Treatment of Gastroenteropancreatic Neuroendocrine Tumors

Cancers, Vol. 4, Pages 100-112: PET-Guided Surgery — High Correlation between Positron Emission Tomography with 11C-5-Hydroxytryptophane (5-HTP) and Surgical Findings in Abdominal Neuroendocrine Tumours

Energies, Vol. 5, Pages 270-290: Experimental Investigation on NOx Reduction by Primary Measures in Biomass Combustion: Straw, Peat, Sewage Sludge, Forest Residues and Wood Pellets

Sensors, Vol. 12, Pages 1720-1737: Signal Processing of MEMS Gyroscope Arrays to Improve Accuracy Using a 1st Order Markov for Rate Signal Modeling

Sensors, Vol. 12, Pages 1702-1719: Social Network Extraction and Analysis Based on Multimodal Dyadic Interaction

Sensors, Vol. 12, Pages 1688-1701: A Web Service-Based Framework Model for People-Centric Sensing Applications Applied to Social Networking

Sensors, Vol. 12, Pages 1657-1687: Noble Metal Nanoparticles for Biosensing Applications

Sensors, Vol. 12, Pages 1648-1656: Protein Reporter Bioassay Systems for the Phenotypic Screening of Candidate Drugs: A Mouse Platform for Anti-Aging Drug Screening

Sensors, Vol. 12, Pages 1625-1647: E-SAP: Efficient-Strong Authentication Protocol for Healthcare Applications Using Wireless Medical Sensor Networks

Sensors, Vol. 12, Pages 1612-1624: Fast Decision Algorithms in Low-Power Embedded Processors for Quality-of-Service Based Connectivity of Mobile Sensors in Heterogeneous Wireless Sensor Networks

Sensors, Vol. 12, Pages 1594-1611: Gait Analysis Using Floor Markers and Inertial Sensors

IJERPH, Vol. 9, Pages 511-520: Mineral Contamination from Cemetery Soils: Case Study of Zandfontein Cemetery, South Africa

Sustainability, Vol. 4, Pages 227-243: Employment Effects of Renewable Energy Expansion on a Regional Level—First Results of a Model-Based Approach for Germany

Molbank, Vol. 2012, Pages M749: Ethyl 3-{2-[(3-Methyl-1H-indol-2-yl)carbonyl]­hydrazinylidene}­butanoate

Membranes, Vol. 2, Pages 16-39: Stimuli Responsive Ionogels for Sensing Applications—An Overview

Cancers, Vol. 4, Pages 88-99: Transition of Treatment for Patients with Extra-Abdominal Desmoid Tumors: Nagoya University Modality

Nutrients, Vol. 4, Pages 78-90: Antiapoptotic and Antiautophagic Effects of Eicosapentaenoic Acid in Cardiac Myoblasts Exposed to Palmitic Acid

Molbank, Vol. 2012, Pages M749: Ethyl 3-{2-[(3-Methyl-1H-indol-2-yl)carbonyl]hydrazinylidene}butanoate