UV Treatment Improves the Biocompatibility and Antibacterial Properties of Crystallized Nanostructured Titanium Surface
Abstract
:1. Introduction
2. Results
2.1. Surface Characterization
2.2. Protein Adsorption
2.3. In Vitro Tests Using RBM Cells
2.4. In Vitro Tests Using Human Umbilical Vein Endothelial Cells (HUVECs)
2.5. In Vitro Antibacterial Tests
2.6. In Vivo Tests Using SD Rats
3. Discussion
4. Materials and Methods
4.1. Sample Preparation
4.2. Surface Characterization
4.3. Protein Adsorption Assay
4.4. Culture of RBM Cells
4.5. In Vitro Tests Using RBM Cells
4.5.1. Cell Viability
4.5.2. Cell Morphology
4.5.3. ALP Activity
4.5.4. OCN Secretion
4.5.5. Mineralization Assay
4.5.6. qRT-PCR
4.6. Culture of HUVECs
4.7. In Vitro Tests Using HUVECs
4.7.1. Cell Viability
4.7.2. Cell Morphology
4.7.3. qRT-PCR
4.8. Bacterial Cultivation
4.9. Antibacterial Activity Assay
4.10. Evaluation of Bacterial Cell Viability by Live/Dead Staining
4.11. Biofilm Formation Assay
4.12. Measurement of ROS Level
4.13. Statistical Analysis
4.14. In Vivo Test Using SD Rats
4.14.1. Animal Model and Surgical Procedures
4.14.2. Micro-CT Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ALP | Alkaline phosphatase |
BMP-2 | Bone morphogenetic protein 2 |
BSA | Bovine serum albumin |
DAPI | 4′,6-Diamidino-2-phenylindole |
DCFH-DA | 2′,7′-Dichlorodihydrofluorescin diacetate |
ELISA | Enzyme-linked immunosorbent assay |
HUVEC | Human umbilical vein endothelial cell |
ICAM-1 | Intercellular adhesion molecule 1 |
OCN | Osteocalcin |
OPN | Osteopontin |
PBS | Phosphate-buffered saline |
qRT-PCR | Quantitative real-time polymerase chain reaction |
Ra | Arithmetic mean roughness |
RBM | Rat bone marrow |
ROS | Reactive oxygen species |
Runx2 | Runt-related transcription factor |
SEM | Scanning electron microscopy |
SPM | Scanning probe microscopy |
TF-XRD | Thin-film X-ray powder diffractometry |
Ti | Titanium |
TiO2 | Titanium dioxide |
TNS | Titanium nanosheet |
UV | Ultraviolet |
XPS | X-ray photoelectron spectroscopy |
XPS | X-ray photoelectron spectroscopy |
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Hatoko, M.; Komasa, S.; Zhang, H.; Sekino, T.; Okazaki, J. UV Treatment Improves the Biocompatibility and Antibacterial Properties of Crystallized Nanostructured Titanium Surface. Int. J. Mol. Sci. 2019, 20, 5991. https://doi.org/10.3390/ijms20235991
Hatoko M, Komasa S, Zhang H, Sekino T, Okazaki J. UV Treatment Improves the Biocompatibility and Antibacterial Properties of Crystallized Nanostructured Titanium Surface. International Journal of Molecular Sciences. 2019; 20(23):5991. https://doi.org/10.3390/ijms20235991
Chicago/Turabian StyleHatoko, Mai, Satoshi Komasa, Honghao Zhang, Tohru Sekino, and Joji Okazaki. 2019. "UV Treatment Improves the Biocompatibility and Antibacterial Properties of Crystallized Nanostructured Titanium Surface" International Journal of Molecular Sciences 20, no. 23: 5991. https://doi.org/10.3390/ijms20235991