Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9
Abstract
:1. Introduction
2. Materials and Methods
2.1. Regents and Instruments
2.2. Strains, Plasmids, and Media
2.3. Isolation, Phylogenetic Analyses of Tannin-Degrading Yeast Strain
2.4. Tannic Acid Tolerance Analyses by A. melanogenum T9
2.5. Gene Expression Level Analyses with qRT-PCR Assay
2.6. Proteins Expression Level Analyses with Label-Free Technology Mass Spectrometry-Based Label-Free Quantitative Proteomics
2.7. Genes Function Analyses by Construction of Mutant Strains
2.8. GAD and Different Tananses Proteins Analyses with Bioinformatics Method
2.9. Statistical Analyses
3. Result
3.1. A. melanogenum T9 Having the Ability of Tannic Acid Degradation
3.2. Analyses of Tannin Tolerance by A. melanogenum T9
3.3. A. melanogenum T9 Growth Process Analyses with Tannic Acid as the Sole Carbon Course
3.4. Bioinformatics Analyses of Tannases and GAD
3.5. Tannic Acid Induced Related Genes Expression Up-Regulation
3.6. tanA and tanB Having Sililar Function on the Tannic Acid Metabolizing
3.7. Gad Was Crucial for on the Tannic Acid Metabolizing and Growth
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Primers | Sequence 5′-3′ |
---|---|
TA5 | CGATTGGAGCACCTTCAACGAGA |
TA3 | CGACCTGAAAGGGATGATGGGAT |
TB5 | TATTCGTGTTGTAGGTCGGGTCA |
TB3 | AGAACGGCACCATTACTGCTCAA′ |
TC5 | GACCTCGACGTAACCAGACCTGA |
TC3 | CAACTGACGATGTTCCTTGCTCC |
GD5 | CAACAAGTTGGAAGCAAGGCAATA |
GD3 | CACAGCACGAGTGAGGTTGGGAT |
Primers | Sequence 5′-3′ |
---|---|
A5F | CCTGGCAACTCGTCCTACAACAT |
A5R | GATCCCCCGAATTAGACCTGCATCTCCTTCAGTCCTT |
A3F | ATGAGCCAACTGTCGCCGAGCCCTACGATTGGAGCACCTT |
A3R | GGTTGAGTAGCGCCAGCGATGTA |
B5F | GTCGATGGAAGCCTTGTCGTGTA |
B5R | GATCCCCCGAATTACACTTATCCTGACCTGACCACCTT |
B3F | ATGAGCCAACTGTCGAAAGGGAGAAACCACCTGGCAATT |
B3R | TCCAACCAGCCATGAGTCACCTC |
G5F | ATGAAGGTTCGCGAGATCTGTGAGG |
G5R | GATCCCCCGAATTAATGTCGACTTGGGAGCCGATGATGC |
G3F | ATGAGCCAACTGTCGAGATGGACAATGACGCCGACTGTCG |
G3R | GATCATCCTCACCAGTCAAATCAGG |
HPT5 | TAATTCGGGGGATCTGGATTTTAGTACTGGA |
HPT3 | CGACAGTTGGCTCATCATCCGTTACATCA |
Protein | Accession Number | Strain | Residues (aa) | MW (KDa) | Signal Peptide |
---|---|---|---|---|---|
TanAp-Like Proteins | |||||
Tannase | KEQ59156.1 | A. melanogenum CBS 110374 | 528 | 57.23 | Yes |
Tannase | XP_013428471.1 | Aureobasidium namibiae CBS 147.97 | 528 | 57.30 | Yes |
Tannase | KEQ86878.1 | Aureobasidium pullulans EXF-150 | 542 | 55.15 | No |
Tannase | OAK97256.1 | Stagonospora sp. SRC1lsM3a | 538 | 56.57 | No |
Tannase | EJD50919.1 | Auricularia subglabra TFB-10046 SS5 | 537 | 53.72 | No |
Tannase | PWO06677.1 | Pyrenophora triticirepentis | 540 | 55.97 | No |
TanBp-like Proteins | |||||
Tannase | KEQ62357.1 | A. melanogenum CBS 110374 | 587 | 63.39 | No |
Tannase | KEQ81812.1 | A. pullulans EXF-150 | 583 | 62.95 | No |
Tannase | EYE96818.1 | Aspergillus ruber CBS 135680 | 579 | 62.76 | No |
Tannase | KXG49722.1 | Penicillium griseofulvum | 580 | 62.85 | Yes |
Tannase | PQE10574.1 | Rutstroemia sp. NJR-2017a BVV2 | 581 | 62.62 | Yes |
Tannase | CDM29520.1 | Penicillium roqueforti FM164 | 579 | 62.57 | Yes |
Tannase | XP_023456645.1 | Cercospora beticola | 609 | 65.65 | Yes |
Tannase | XP_016598960.1 | Penicillium expansum | 580 | 62.83 | Yes |
Tannase | GAQ07635.1 | Aspergillus lentulus | 588 | 63.45 | Yes |
Tannase | CRL25663.1 | Penicillium camemberti | 580 | 62.86 | Yes |
Tannase | OKP13191.1 | Penicillium subrubescens | 589 | 64.00 | No |
Tannase | RSL58341.1 | Fusarium sp. AF-6 | 581 | 63.20 | Yes |
TanCp-like proteins | |||||
Tannase | KEQ62631.1 | A. melanogenum CBS 110374 | 508 | 54.68 | Yes |
Tannase | KEQ81081.1 | A. pullulans EXF-150 | 496 | 53.94 | No |
Tannase | XP_020125410.1 | Diplodia corticola | 542 | 58.16 | No |
Tannase | PVH72277.1 | Cadophora sp. DSE1049 | 513 | 54.75 | Yes |
Tannase | XP_018037725.1 | Paraphaeosphaeria sporulosa | 508 | 55.17 | Yes |
Tannase | KXH32567.1 | Colletotrichum nymphaeae SA-01 | 468 | 51.07 | No |
Tannase | XP_018070318.1 | Phialocephala scopiformis | 405 | 43.54 | No |
Tannase | ORY60088.1 | Pseudomassariella vexata | 451 | 49.84 | No |
Tannase | POS72090.1 | Diaporthe helianthi | 750 | 81.47 | Yes |
Tannase | KKP04619.1 | Trichoderma harzianum | 466 | 50.71 | Yes |
Tannase | KPA35627.1 | Fusarium langsethiae | 514 | 56.00 | Yes |
Gene | Protein | Gene Transcription Level Change (Fold) | Protein Translation Level Change (Fold) |
---|---|---|---|
tanA | TanAp | 32.00 ± 3.6 | 8.22 |
tanB | TanBp | 64.70 ± 5.2 | 332.00 |
tanC | TanCp | 0.74 ± 0.2 | - |
gad | GAD | 3.21 ± 0.3 | - |
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Zhang, L.-L.; Li, J.; Wang, Y.-L.; Liu, S.; Wang, Z.-P.; Yu, X.-J. Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9. Biomolecules 2019, 9, 439. https://doi.org/10.3390/biom9090439
Zhang L-L, Li J, Wang Y-L, Liu S, Wang Z-P, Yu X-J. Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9. Biomolecules. 2019; 9(9):439. https://doi.org/10.3390/biom9090439
Chicago/Turabian StyleZhang, Lin-Lin, Jie Li, Yi-Lin Wang, Song Liu, Zhi-Peng Wang, and Xin-Jun Yu. 2019. "Integrated Approaches to Reveal Genes Crucial for Tannin Degradation in Aureobasidium melanogenum T9" Biomolecules 9, no. 9: 439. https://doi.org/10.3390/biom9090439