E-Mail Alert

Add your e-mail address to receive forthcoming issues of this journal:

Journal Browser

Journal Browser

Special Issue "Breakthroughs in Viral Replication"

A special issue of Viruses (ISSN 1999-4915).

Deadline for manuscript submissions: 25 September 2018

Special Issue Editors

Guest Editor
Dr. Eric O. Freed

Director, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Building 535, Room 110, Frederick, MD 21702-1201, USA
Website | E-Mail
Guest Editor
Dr. Albert Bosch

Enteric Virus Laboratory, School of Biology, University of Barcelona, Spain
Website | E-Mail

Special Issue Information

Dear Colleagues,

This Special Issue is related to the conference "Viruses 2018–Breakthroughs in Viral Replication" which will be held at the University of Barcelona, Barcelona, Spain, 7–9 February, 2018.

Viral infections continue to cause substantial morbidity and mortality in humans and all other living organisms. Wave upon wave of viral pandemics are having a major impact on global health. At the same time, great strides are being made in understanding at the molecular, cellular, organismal and epidemiological level how viruses replicate and induce pathology. This increase in knowledge is being translated into new treatments and vaccines for many viral infections.

Symposium participants, as well as all researchers working in the field, are cordially invited to contribute original research papers or reviews to this Special Issue of Viruses, which will focus on the progress being made in understanding virus replication, structure, transmission, pathogenesis, and antiviral immunity.

Dr. Eric O. Freed
Dr. Albert Bosch
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Viruses is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (1 paper)

View options order results:
result details:
Displaying articles 1-1
Export citation of selected articles as:

Research

Open AccessArticle Analyses of Tissue Culture Adaptation of Human Herpesvirus-6A by Whole Genome Deep Sequencing Redefines the Reference Sequence and Identifies Virus Entry Complex Changes
Viruses 2018, 10(1), 16; https://doi.org/10.3390/v10010016
Received: 30 November 2017 / Revised: 23 December 2017 / Accepted: 26 December 2017 / Published: 31 December 2017
PDF Full-text (1338 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Tissue-culture adaptation of viruses can modulate infection. Laboratory passage and bacterial artificial chromosome (BAC)mid cloning of human cytomegalovirus, HCMV, resulted in genomic deletions and rearrangements altering genes encoding the virus entry complex, which affected cellular tropism, virulence, and vaccine development. Here, we analyse
[...] Read more.
Tissue-culture adaptation of viruses can modulate infection. Laboratory passage and bacterial artificial chromosome (BAC)mid cloning of human cytomegalovirus, HCMV, resulted in genomic deletions and rearrangements altering genes encoding the virus entry complex, which affected cellular tropism, virulence, and vaccine development. Here, we analyse these effects on the reference genome for related betaherpesviruses, Roseolovirus, human herpesvirus 6A (HHV-6A) strain U1102. This virus is also naturally “cloned” by germline subtelomeric chromosomal-integration in approximately 1% of human populations, and accurate references are key to understanding pathological relationships between exogenous and endogenous virus. Using whole genome next-generation deep-sequencing Illumina-based methods, we compared the original isolate to tissue-culture passaged and the BACmid-cloned virus. This re-defined the reference genome showing 32 corrections and 5 polymorphisms. Furthermore, minor variant analyses of passaged and BACmid virus identified emerging populations of a further 32 single nucleotide polymorphisms (SNPs) in 10 loci, half non-synonymous indicating cell-culture selection. Analyses of the BAC-virus genome showed deletion of the BAC cassette via loxP recombination removing green fluorescent protein (GFP)-based selection. As shown for HCMV culture effects, select HHV-6A SNPs mapped to genes encoding mediators of virus cellular entry, including virus envelope glycoprotein genes gB and the gH/gL complex. Comparative models suggest stabilisation of the post-fusion conformation. These SNPs are essential to consider in vaccine-design, antimicrobial-resistance, and pathogenesis. Full article
(This article belongs to the Special Issue Breakthroughs in Viral Replication)
Figures

Figure 1

Back to Top