Special Issue "Analytical Tools to Study Biomarkers"

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (30 November 2017)

Special Issue Editor

Guest Editor
Dr. Sandrine Parrot

NeuroDialyTics Unit—INSERM U1028—CNRS UMR 5292, Lyon Neuroscience Research Center (CRNL), University Claude Bernard Lyon 1, 7 Rue Guillaume Paradin, F-69372 LYON Cedex 08, France
Website | E-Mail
Phone: +33-4-78-77-75-58
Interests: neurochemistry; neurotransmitter monitoring; methodological development; brain sampling; small volume analysis; ultra-high performance liquid chromatography; capillary electrophoresis

Special Issue Information

Dear Colleagues,

Analytical tools for the detection and quantification of biomarkers are, nowadays, very challenging since these compounds, present in accessible body fluids or tissues, can be used to reveal and diagnose alterations or dysfunctions in many physio-pathological states. In this context, sampling methods, pre-analytical treatments, separation and detection approaches are crucial to relevantly study biomarkers in either human diseases or experimental models.

We would like to invite colleagues to contribute with original research articles and reviews to the present Special Issue that aims to show the broad spectrum of analytical possibilities in biomarker monitoring. We are greatly interested in papers describing new methods with a focus on robustness in case of highly original approaches. The development of methods dealing with new potential biomarkers in preclinical or diagnosis studies in any field are also welcome.

Dr. Sandrine Parrot
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 350 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biomarkers
  • separation
  • sampling
  • pretreatment
  • separation
  • detection
  • diagnosis
  • body fluids
  • tissues

Published Papers (1 paper)

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Research

Open AccessArticle Advances and Pitfalls in the Capillary Electrophoresis Analysis of Aggregates of Beta Amyloid Peptides
Received: 20 October 2017 / Revised: 1 December 2017 / Accepted: 14 December 2017 / Published: 22 December 2017
PDF Full-text (1900 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Alzheimer’s disease is characterized by the accumulation of brain amyloid plaques composed of aggregates of amyloid β (Aβ) peptides. The present paper describes a novel and easy-to-run capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method for the specific analysis of fibrillar forms of
[...] Read more.
Alzheimer’s disease is characterized by the accumulation of brain amyloid plaques composed of aggregates of amyloid β (Aβ) peptides. The present paper describes a novel and easy-to-run capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method for the specific analysis of fibrillar forms of Aβ aggregates obtained after in vitro incubation of Aβ 1-40 monomer. For that purpose, an affinity CE-LIF approach in which the ligand thioflavine T was added to the running buffer has been used, leading to the separation and detection of various fibrillar aggregates which migrated as spikes. The procedure has been optimized to get spikes only corresponding to Aβ aggregates, through the careful elimination of interfering factors and the electrophoretic validation of the link between spikes and particulate material. This method exhibited semi-quantification capabilities, led to the separation of Aβ fibrillar aggregates of different sizes and showed that highly concentrated solutions of Aβ peptides led to the formation of aggregates of larger size than lower-concentrated solution did. Advances brought by this method as well as future development needed to overcome its present limitations are discussed. Full article
(This article belongs to the Special Issue Analytical Tools to Study Biomarkers)
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