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High-Throughput 2018, 7(3), 26; https://doi.org/10.3390/ht7030026

Transcriptional Identification of Related Proteins in the Immune System of the Crayfish Procambarus clarkii

1
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, C. P. 04510 Ciudad de México, Mexico
2
Laboratorio de Medicina Genómica, Hospital Regional “Primero de Octubre” ISSSTE, Av. Instituto Politécnico Nacional 1669, 07300 Ciudad de México, Mexico
3
Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, San Pedro Zacatenco, 07360 Ciudad de México, Mexico
*
Author to whom correspondence should be addressed.
Received: 4 August 2018 / Revised: 5 September 2018 / Accepted: 5 September 2018 / Published: 12 September 2018
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Abstract

The freshwater crayfish Procambarus clarkii is an animal model employed for physiological and immunological studies and is also of great economic importance in aquaculture. Although it is a species of easy husbandry, a high percentage of its production is lost annually as a result of infectious diseases. Currently, genetic information about the immune system of crustaceans is limited. Therefore, we used the abdominal nerve cord from P. clarkii to obtain its transcriptome using Next Generation Sequencing (NGS) to identify proteins that participate in the immune system. The reads were assembled de novo and consensus sequences with more than 3000 nucleotides were selected for analysis. The transcripts of the sequences of RNA were edited for annotation and sent to the GenBank database of the National Center for Biotechnology Information (NCBI). We made a list of accession numbers of the sequences which were organized by the putative role of the immune system pathway in which they participate. In this work, we report on 80 proteins identified from the transcriptome of crayfish related to the immune system, 74 of them being the first reported for P. clarkii. We hope that the knowledge of these sequences will contribute significantly to the development of future studies of the immune system in crustaceans. View Full-Text
Keywords: crayfish; Procambarus clarkii; transcriptome; immunity; crustacean crayfish; Procambarus clarkii; transcriptome; immunity; crustacean
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Calderón-Rosete, G.; González-Barrios, J.A.; Lara-Lozano, M.; Piña-Leyva, C.; Rodríguez-Sosa, L. Transcriptional Identification of Related Proteins in the Immune System of the Crayfish Procambarus clarkii. High-Throughput 2018, 7, 26.

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