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High-Throughput 2018, 7(1), 7; https://doi.org/10.3390/ht7010007

When Transcriptomics and Metabolomics Work Hand in Hand: A Case Study Characterizing Plant CDF Transcription Factors

1
Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)—Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28223 Pozuelo de Alarcón (Madrid), Spain
2
Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Received: 28 December 2017 / Revised: 21 February 2018 / Accepted: 21 February 2018 / Published: 28 February 2018
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Abstract

Over the last three decades, novel “omics” platform technologies for the sequencing of DNA and complementary DNA (cDNA) (RNA-Seq), as well as for the analysis of proteins and metabolites by mass spectrometry, have become more and more available and increasingly found their way into general laboratory life. With this, the ability to generate highly multivariate datasets on the biological systems of choice has increased tremendously. However, the processing and, perhaps even more importantly, the integration of “omics” datasets still remains a bottleneck, although considerable computational and algorithmic advances have been made in recent years. In this mini-review, we use a number of recent “multi-omics” approaches realized in our laboratories as a common theme to discuss possible pitfalls of applying “omics” approaches and to highlight some useful tools for data integration and visualization in the form of an exemplified case study. In the selected example, we used a combination of transcriptomics and metabolomics alongside phenotypic analyses to functionally characterize a small number of Cycling Dof Transcription Factors (CDFs). It has to be remarked that, even though this approach is broadly used, the given workflow is only one of plenty possible ways to characterize target proteins. View Full-Text
Keywords: secondary metabolites; metabolomics; systems biology; plant biology secondary metabolites; metabolomics; systems biology; plant biology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Pérez-Alonso, M.-M.; Carrasco-Loba, V.; Medina, J.; Vicente-Carbajosa, J.; Pollmann, S. When Transcriptomics and Metabolomics Work Hand in Hand: A Case Study Characterizing Plant CDF Transcription Factors. High-Throughput 2018, 7, 7.

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