Leishmania spp. Detection Using a Surface Plasmon Resonance Biosensor†
AbstractThe detection of Leishmania spp. through a disposable surface plasmon resonance (SPR) biochip, using mono- and polychromatic sources, is presented here. A highly specific and sensitive-constrained synthetic peptide (LC2) derived from a mimotope of Leishmania chagasi antigen was immobilized on the sensing region using a simple protocol. ELISA- and PCR-confirmed negative and positive sera of Leishmania chagasi infected patients were used. Detection of positive IgG showed a fast response with good sensitivity and specificity for the proposed method.
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Ferreira, E.; Lima, J.; Alves-Balvedi, R.P.; Bonan, P.R.; Medeiros, E.; Goulart, L.; Lima, A.; Neff, H.; Oliveira, L.; Castellano, L.; Araújo, A.; Moreira, C. Leishmania spp. Detection Using a Surface Plasmon Resonance Biosensor. Proceedings 2017, 1, 536.
Ferreira E, Lima J, Alves-Balvedi RP, Bonan PR, Medeiros E, Goulart L, Lima A, Neff H, Oliveira L, Castellano L, Araújo A, Moreira C. Leishmania spp. Detection Using a Surface Plasmon Resonance Biosensor. Proceedings. 2017; 1(4):536.Chicago/Turabian Style
Ferreira, Edlaine; Lima, Jefferson; Alves-Balvedi, Renata Pereira; Bonan, Paulo Rogério; Medeiros, Eliton; Goulart, Luiz; Lima, Antonio; Neff, Helmut; Oliveira, Leiva; Castellano, Lucio; Araújo, Artur; Moreira, Cleumar. 2017. "Leishmania spp. Detection Using a Surface Plasmon Resonance Biosensor." Proceedings 1, no. 4: 536.
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